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基本情報
登録情報 | データベース: EMDB / ID: EMD-23121 | |||||||||
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タイトル | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | |||||||||
![]() | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | |||||||||
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![]() | Cryo-EM / Mammalian / mito-ribosome / mtEFg2 / mtRRF / RIBOSOME | |||||||||
機能・相同性 | ![]() mitochondrial transcription / mitochondrial translational termination / mitochondrial translational elongation / translation release factor activity, codon nonspecific / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / translation release factor activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase ...mitochondrial transcription / mitochondrial translational termination / mitochondrial translational elongation / translation release factor activity, codon nonspecific / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / translation release factor activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / mitochondrial ribosome / ribosome disassembly / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドリボヌクレアーゼ / mitochondrial small ribosomal subunit / peptidyl-tRNA hydrolase activity / mitochondrial translation / ribosomal large subunit binding / anatomical structure morphogenesis / RNA processing / rescue of stalled ribosome / Mitochondrial protein degradation / cellular response to leukemia inhibitory factor / fibrillar center / cell junction / large ribosomal subunit / double-stranded RNA binding / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / endonuclease activity / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / mitochondrial inner membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / structural constituent of ribosome / mitochondrial matrix / protein domain specific binding / translation / ribonucleoprotein complex / nucleotide binding / GTPase activity / mRNA binding / apoptotic process / GTP binding / positive regulation of DNA-templated transcription / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.15 Å | |||||||||
![]() | Agrawal E / Koripella R | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Distinct mechanisms of the human mitoribosome recycling and antibiotic resistance. 著者: Ravi Kiran Koripella / Ayush Deep / Ekansh K Agrawal / Pooja Keshavan / Nilesh K Banavali / Rajendra K Agrawal / ![]() 要旨: Ribosomes are recycled for a new round of translation initiation by dissociation of ribosomal subunits, messenger RNA and transfer RNA from their translational post-termination complex. Here we ...Ribosomes are recycled for a new round of translation initiation by dissociation of ribosomal subunits, messenger RNA and transfer RNA from their translational post-termination complex. Here we present cryo-EM structures of the human 55S mitochondrial ribosome (mitoribosome) and the mitoribosomal large 39S subunit in complex with mitoribosome recycling factor (RRF) and a recycling-specific homolog of elongation factor G (EF-G2). These structures clarify an unusual role of a mitochondria-specific segment of RRF, identify the structural distinctions that confer functional specificity to EF-G2, and show that the deacylated tRNA remains with the dissociated 39S subunit, suggesting a distinct sequence of events in mitoribosome recycling. Furthermore, biochemical and structural analyses reveal that the molecular mechanism of antibiotic fusidic acid resistance for EF-G2 is markedly different from that of mitochondrial elongation factor EF-G1, suggesting that the two human EF-Gs have evolved diversely to negate the effect of a bacterial antibiotic. | |||||||||
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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-EMDBアーカイブ
マップデータ | ![]() | 227 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 70.3 KB 70.3 KB | 表示 表示 | ![]() |
画像 | ![]() | 171.9 KB | ||
Filedesc metadata | ![]() | 15.6 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7l20MC ![]() 7l08C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data size: 2.3 TB Data #1: Aligned single-frame particles of human mitochondrial 55S-EF-Gmt complex [picked particles - single frame - processed]) |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM structure of the human 39S mitoribosomal subunit in complex with RRFmt and EF-G2mt. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.07325 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
+全体 : Cryo-EM structure of Mammalian Mitochondrial ribosome complex wit...
+超分子 #1: Cryo-EM structure of Mammalian Mitochondrial ribosome complex wit...
+分子 #1: 16S rRNA mitochondrial
+分子 #2: tRNAval
+分子 #3: 39S ribosomal protein L2, mitochondrial
+分子 #4: 39S ribosomal protein L4, mitochondrial
+分子 #5: 39S ribosomal protein L9, mitochondrial
+分子 #6: 39S ribosomal protein L13, mitochondrial
+分子 #7: 39S ribosomal protein L14, mitochondrial
+分子 #8: 39S ribosomal protein L15, mitochondrial
+分子 #9: 39S ribosomal protein L17, mitochondrial
+分子 #10: 39S ribosomal protein L20, mitochondrial
+分子 #11: 39S ribosomal protein L21, mitochondrial
+分子 #12: 39S ribosomal protein L22, mitochondrial
+分子 #13: 39S ribosomal protein L27, mitochondrial
+分子 #14: 39S ribosomal protein L28, mitochondrial
+分子 #15: 39S ribosomal protein L47, mitochondrial
+分子 #16: 39S ribosomal protein L30, mitochondrial
+分子 #17: 39S ribosomal protein L32, mitochondrial
+分子 #18: 39S ribosomal protein L33, mitochondrial
+分子 #19: 39S ribosomal protein L34, mitochondrial
+分子 #20: 39S ribosomal protein L35, mitochondrial
+分子 #21: 39S ribosomal protein L36, mitochondrial
+分子 #22: 39S ribosomal protein L40, mitochondrial
+分子 #23: 39S ribosomal protein L43, mitochondrial
+分子 #24: 39S ribosomal protein L46, mitochondrial
+分子 #25: 39S ribosomal protein L49, mitochondrial
+分子 #26: 39S ribosomal protein L51, mitochondrial
+分子 #27: cDNA FLJ76418, highly similar to Homo sapiens mitochondrial ribos...
+分子 #28: 39S ribosomal protein L55, mitochondrial
+分子 #29: Ribosomal protein 63, mitochondrial
+分子 #30: Growth arrest and DNA damage-inducible proteins-interacting protein 1
+分子 #31: 39S ribosomal protein S18a, mitochondrial
+分子 #32: 39S ribosomal protein L11, mitochondrial
+分子 #33: 39S ribosomal protein L10, mitochondrial
+分子 #34: 39S ribosomal protein L16, mitochondrial
+分子 #35: Mitochondrial ribosomal protein L18, isoform CRA_b
+分子 #36: 39S ribosomal protein L23, mitochondrial
+分子 #37: 39S ribosomal protein L24, mitochondrial
+分子 #38: 39S ribosomal protein L3, mitochondrial
+分子 #39: 39S ribosomal protein L37, mitochondrial
+分子 #40: 39S ribosomal protein L38, mitochondrial
+分子 #41: 39S ribosomal protein L39, mitochondrial
+分子 #42: 39S ribosomal protein L41, mitochondrial
+分子 #43: 39S ribosomal protein L42, mitochondrial
+分子 #44: 39S ribosomal protein L44, mitochondrial
+分子 #45: 39S ribosomal protein L45, mitochondrial
+分子 #46: 39S ribosomal protein L48, mitochondrial
+分子 #47: 39S ribosomal protein L50, mitochondrial
+分子 #48: 39S ribosomal protein L53, mitochondrial
+分子 #49: 39S ribosomal protein L54, mitochondrial
+分子 #50: Peptidyl-tRNA hydrolase ICT1, mitochondrial
+分子 #51: 39S ribosomal protein S30, mitochondrial
+分子 #52: 39S ribosomal protein L19, mitochondrial
+分子 #53: 39 S P-site finger
+分子 #54: 39S ribosomal protein L12, mitochondrial
+分子 #55: Ribosome-recycling factor, mitochondrial
+分子 #56: Ribosome-releasing factor 2, mitochondrial
+分子 #57: MAGNESIUM ION
+分子 #58: ZINC ION
+分子 #59: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: DIRECT ELECTRON DE-16 (4k x 4k) 平均電子線量: 71.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: OTHER |
電子光学系 | 照射モード: OTHER / 撮影モード: OTHER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: PDB ENTRY PDBモデル - PDB ID: |
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最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.15 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 132008 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
-原子モデル構築 1
精密化 | プロトコル: OTHER |
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得られたモデル | ![]() PDB-7l20: |