National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
75N93019C00051
米国
引用
ジャーナル: Cell Rep / 年: 2021 タイトル: Polyclonal epitope mapping reveals temporal dynamics and diversity of human antibody responses to H5N1 vaccination. 著者: Julianna Han / Aaron J Schmitz / Sara T Richey / Ya-Nan Dai / Hannah L Turner / Bassem M Mohammed / Daved H Fremont / Ali H Ellebedy / Andrew B Ward / 要旨: Novel influenza A virus (IAV) strains elicit recall immune responses to conserved epitopes, making them favorable antigenic choices for universal influenza virus vaccines. Evaluating these immunogens ...Novel influenza A virus (IAV) strains elicit recall immune responses to conserved epitopes, making them favorable antigenic choices for universal influenza virus vaccines. Evaluating these immunogens requires a thorough understanding of the antigenic sites targeted by the polyclonal antibody (pAb) response, which single-particle electron microscopy (EM) can sensitively detect. In this study, we employ EM polyclonal epitope mapping (EMPEM) to extensively characterize the pAb response to hemagglutinin (HA) after H5N1 immunization in humans. Cross-reactive pAbs originating from memory B cells immediately bound the stem of HA and persisted for more than a year after vaccination. In contrast, de novo pAb responses to multiple sites on the head of HA, targeting previously determined key neutralizing sites on H5 HA, expanded after the second immunization and waned quickly. Thus, EMPEM provides a robust tool for comprehensively tracking the specificity and durability of immune responses elicited by novel universal influenza vaccine candidates.
ダウンロード / ファイル: emd_22560.map.gz / 形式: CCP4 / 大きさ: 15.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Polyclonal immune complex of Fab binding the receptor binding site region of H5 HA from serum of subject 43 at day 100
ボクセルのサイズ
X=Y=Z: 1.98 Å
密度
表面レベル
登録者による: 0.0188 / ムービー #1: 0.0188
最小 - 最大
-0.040136993 - 0.11461521
平均 (標準偏差)
0.00014542796 (±0.0054220925)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
160
160
160
Spacing
160
160
160
セル
A=B=C: 316.8 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.98
1.98
1.98
M x/y/z
160
160
160
origin x/y/z
0.000
0.000
0.000
length x/y/z
316.800
316.800
316.800
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
160
160
160
D min/max/mean
-0.040
0.115
0.000
-
添付データ
-
試料の構成要素
-
全体 : Polyclonal immune complex of Fab binding the receptor binding sit...
全体
名称: Polyclonal immune complex of Fab binding the receptor binding site region of H5 HA from serum of subject 43 at day 100
要素
複合体: Polyclonal immune complex of Fab binding the receptor binding site region of H5 HA from serum of subject 43 at day 100
-
超分子 #1: Polyclonal immune complex of Fab binding the receptor binding sit...
超分子
名称: Polyclonal immune complex of Fab binding the receptor binding site region of H5 HA from serum of subject 43 at day 100 タイプ: complex / ID: 1 / 親要素: 0
由来(天然)
生物種: Homo sapiens (ヒト) / 細胞中の位置: PBMC
-
実験情報
-
構造解析
手法
ネガティブ染色法
解析
単粒子再構成法
試料の集合状態
particle
-
試料調製
緩衝液
pH: 7.4
染色
タイプ: NEGATIVE / 材質: 2% w/v uranyl formate
-
電子顕微鏡法
顕微鏡
FEI TALOS ARCTICA
撮影
フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 25.0 e/Å2