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- EMDB-22260: Structure of P5A-ATPase Spf1, Apo form -

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Basic information

Entry
Database: EMDB / ID: EMD-22260
TitleStructure of P5A-ATPase Spf1, Apo form
Map dataP5A-ATPase Spf1, Apo form
Sample
  • Complex: P5A-ATPase Spf1 Apo form
    • Protein or peptide: P5A-type ATPase
  • Ligand: DODECYL-BETA-D-MALTOSIDE
KeywordsP-type ATPase / transmembrane helix dislocase / protein quality control / endoplasmic reticulum / TRANSPORT PROTEIN
Function / homology
Function and homology information


extraction of mislocalized protein from ER membrane / membrane protein dislocase activity / Ion transport by P-type ATPases / intracellular manganese ion homeostasis / sterol homeostasis / Translocases; Catalysing the translocation of amino acids and peptides; Linked to the hydrolysis of a nucleoside triphosphate / P-type ion transporter activity / ATPase-coupled monoatomic cation transmembrane transporter activity / cis-Golgi network / phosphatidylinositol-4-phosphate binding ...extraction of mislocalized protein from ER membrane / membrane protein dislocase activity / Ion transport by P-type ATPases / intracellular manganese ion homeostasis / sterol homeostasis / Translocases; Catalysing the translocation of amino acids and peptides; Linked to the hydrolysis of a nucleoside triphosphate / P-type ion transporter activity / ATPase-coupled monoatomic cation transmembrane transporter activity / cis-Golgi network / phosphatidylinositol-4-phosphate binding / protein hexamerization / protein unfolding / transmembrane transport / intracellular calcium ion homeostasis / protein transport / endoplasmic reticulum membrane / endoplasmic reticulum / ATP hydrolysis activity / mitochondrion / ATP binding / membrane / metal ion binding
Similarity search - Function
: / P-type ATPase, subfamily V / Cation transport ATPase (P-type) / E1-E2 ATPase / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase ...: / P-type ATPase, subfamily V / Cation transport ATPase (P-type) / E1-E2 ATPase / P-type ATPase, haloacid dehalogenase domain / P-type ATPase, phosphorylation site / P-type ATPase, cytoplasmic domain N / E1-E2 ATPases phosphorylation site. / P-type ATPase, A domain superfamily / P-type ATPase / P-type ATPase, transmembrane domain superfamily / HAD superfamily / HAD-like superfamily
Similarity search - Domain/homology
Endoplasmic reticulum transmembrane helix translocase
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsPark E / Sim SI
Funding support United States, 1 items
OrganizationGrant numberCountry
Other private United States
CitationJournal: Science / Year: 2020
Title: The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.
Authors: Michael J McKenna / Sue Im Sim / Alban Ordureau / Lianjie Wei / J Wade Harper / Sichen Shao / Eunyong Park /
Abstract: Organelle identity depends on protein composition. How mistargeted proteins are selectively recognized and removed from organelles is incompletely understood. Here, we found that the orphan P5A- ...Organelle identity depends on protein composition. How mistargeted proteins are selectively recognized and removed from organelles is incompletely understood. Here, we found that the orphan P5A-adenosine triphosphatase (ATPase) transporter ATP13A1 (Spf1 in yeast) directly interacted with the transmembrane segment (TM) of mitochondrial tail-anchored proteins. P5A-ATPase activity mediated the extraction of mistargeted proteins from the endoplasmic reticulum (ER). Cryo-electron microscopy structures of Spf1 revealed a large, membrane-accessible substrate-binding pocket that alternately faced the ER lumen and cytosol and an endogenous substrate resembling an α-helical TM. Our results indicate that the P5A-ATPase could dislocate misinserted hydrophobic helices flanked by short basic segments from the ER. TM dislocation by the P5A-ATPase establishes an additional class of P-type ATPase substrates and may correct mistakes in protein targeting or topogenesis.
History
DepositionJun 30, 2020-
Header (metadata) releaseSep 23, 2020-
Map releaseSep 23, 2020-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.44
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.44
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6xmp
  • Surface level: 0.44
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22260.png

Downloads & links

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Map

FileDownload / File: emd_22260.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationP5A-ATPase Spf1, Apo form
Voxel sizeX=Y=Z: 1.14 Å
Density
Contour LevelBy AUTHOR: 0.44 / Movie #1: 0.44
Minimum - Maximum-0.8040904 - 1.8895591
Average (Standard dev.)0.0014352299 (±0.059952214)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 291.84 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.141.141.14
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z291.840291.840291.840
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ400400400
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.8041.8900.001

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Supplemental data

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Mask #1

Fileemd_22260_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: P5A-ATPase Spf1, Apo form

Fileemd_22260_additional.map
AnnotationP5A-ATPase Spf1, Apo form
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: P5A-ATPase Spf1, Apo form

Fileemd_22260_half_map_1.map
AnnotationP5A-ATPase Spf1, Apo form
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: P5A-ATPase Spf1, Apo form

Fileemd_22260_half_map_2.map
AnnotationP5A-ATPase Spf1, Apo form
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : P5A-ATPase Spf1 Apo form

EntireName: P5A-ATPase Spf1 Apo form
Components
  • Complex: P5A-ATPase Spf1 Apo form
    • Protein or peptide: P5A-type ATPase
  • Ligand: DODECYL-BETA-D-MALTOSIDE

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Supramolecule #1: P5A-ATPase Spf1 Apo form

SupramoleculeName: P5A-ATPase Spf1 Apo form / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: BY4741

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Macromolecule #1: P5A-type ATPase

MacromoleculeName: P5A-type ATPase / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
EC number: Translocases; Catalysing the translocation of inorganic cations; Linked to the hydrolysis of a nucleoside triphosphate
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 137.573156 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae BY4741 (yeast)
SequenceString: MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYST TKNVNEATHI LIYTTPNNGS DGIVEIQRVT EAGSLQTFFQ FQKKRFLWHE NEQVFSSPKF LVDESPKIGD F QKCKGHSG ...String:
MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYST TKNVNEATHI LIYTTPNNGS DGIVEIQRVT EAGSLQTFFQ FQKKRFLWHE NEQVFSSPKF LVDESPKIGD F QKCKGHSG DLTHLKRLYG ENSFDIPIPT FMELFKEHAV APLFVFQVFC VALWLLDEFW YYSLFNLFMI ISMEAAAVFQ RL TALKEFR TMGIKPYTIN VFRNKKWVAL QTNELLPMDL VSITRTAEES AIPCDLILLD GSAIVNEAML SGESTPLLKE SIK LRPSED NLQLDGVDKI AVLHGGTKAL QVTPPEHKSD IPPPPDGGAL AIVTKTGFET SQGSLVRVMI YSAERVSVDN KEAL MFILF LLIFAVIASW YVWVEGTKMG RIQSKLILDC ILIITSVVPP ELPMELTMAV NSSLAALAKF YVYCTEPFRI PFAGR IDVC CFDKTGTLTG EDLVFEGLAG ISADSENIRH LYSAAEAPES TILVIGAAHA LVKLEDGDIV GDPMEKATLK AVGWAV ERK NSNYREGTGK LDIIRRFQFS SALKRSASIA SHNDALFAAV KGAPETIRER LSDIPKNYDE IYKSFTRSGS RVLALAS KS LPKMSQSKID DLNRDDVESE LTFNGFLIFH CPLKDDAIET IKMLNESSHR SIMITGDNPL TAVHVAKEVG IVFGETLI L DRAGKSDDNQ LLFRDVEETV SIPFDPSKDT FDHSKLFDRY DIAVTGYALN ALEGHSQLRD LLRHTWVYAR VSPSQKEFL LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNP HYLKALESKG TVITPEIRKA VEEANSKPVE VIKPNGLSEK KPADLASLLL NSAGDAQGDE APALKLGDAS C AAPFTSKL ANVSAVTNII RQGRCALVNT IQMYKILALN CLISAYSLSI IYMAGVKFGD GQATVSGLLL SVCFLSISRG KP LEKLSKQ RPQSGIFNVY IMGSILSQFA VHIATLVYIT TEIYKLEPRE PQVDLEKEFA PSLLNTGIFI IQLVQQVSTF AVN YQGEPF RENIRSNKGM YYGLLGVTGL ALASATEFLP ELNEAMKFVP MTDDFKIKLT LTLLLDFFGS WGVEHFFKFF FMDD KPSDI SVQQVKIASK GATGGSTAGG ATTASGTGEN LYFQ

UniProtKB: Endoplasmic reticulum transmembrane helix translocase

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Macromolecule #2: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 2 / Number of copies: 12 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 35 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 776554
Startup modelType of model: OTHER / Details: Ab initio reconstruction in cryoSPARC
Initial angle assignmentType: NOT APPLICABLE
Final 3D classificationSoftware - Name: cryoSPARC (ver. 2.12.4)
Final angle assignmentType: OTHER / Software - Name: cryoSPARC (ver. 2.12.4) / Details: Non-uniform refinement in cryoSPARC
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 2.12.4) / Number images used: 143694
FSC plot (resolution estimation)

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