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- EMDB-21514: map of the mammalian Mediator complex -

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IDまたはキーワード:

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基本情報

登録情報
データベース: EMDB / ID: EMD-21514
タイトルmap of the mammalian Mediator complex
マップデータMammalian Mediator complex
試料
  • 複合体: Mouse mediator complex
    • タンパク質・ペプチド: x 25種
キーワードmouse mediator / complex / GENE REGULATION
機能・相同性
機能・相同性情報


positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / core mediator complex / nuclear retinoic acid receptor binding / mediator complex / nuclear thyroid hormone receptor binding / limb development / cortical actin cytoskeleton / skeletal muscle cell differentiation / somatic stem cell population maintenance ...positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / core mediator complex / nuclear retinoic acid receptor binding / mediator complex / nuclear thyroid hormone receptor binding / limb development / cortical actin cytoskeleton / skeletal muscle cell differentiation / somatic stem cell population maintenance / blastocyst development / positive regulation of transcription initiation by RNA polymerase II / ubiquitin ligase complex / nuclear retinoid X receptor binding / RNA polymerase II preinitiation complex assembly / negative regulation of fibroblast proliferation / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / transcription coactivator binding / ubiquitin protein ligase activity / actin binding / transcription regulator complex / transcription by RNA polymerase II / cytoskeleton / transcription coactivator activity / protein ubiquitination / nuclear body / positive regulation of gene expression / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / nucleolus / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / membrane / cytosol / cytoplasm
類似検索 - 分子機能
Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa ...Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / : / : / : / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 14, RM2 / Mediator of RNA polymerase II transcription subunit 14, RM8 / Mediator of RNA polymerase II transcription subunit 14, RM6 / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / : / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
類似検索 - ドメイン・相同性
Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 ...Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 20
類似検索 - 構成要素
生物種Mus musculus (ハツカネズミ)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.02 Å
データ登録者Zhao H / Young N / Asturias F
資金援助 米国, 2件
OrganizationGrant number国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-67167 米国
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS) 米国
引用ジャーナル: Cell / 年: 2019
タイトル: A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers.
著者: Laila El Khattabi / Haiyan Zhao / Jens Kalchschmidt / Natalie Young / Seolkyoung Jung / Peter Van Blerkom / Philippe Kieffer-Kwon / Kyong-Rim Kieffer-Kwon / Solji Park / Xiang Wang / Jordan ...著者: Laila El Khattabi / Haiyan Zhao / Jens Kalchschmidt / Natalie Young / Seolkyoung Jung / Peter Van Blerkom / Philippe Kieffer-Kwon / Kyong-Rim Kieffer-Kwon / Solji Park / Xiang Wang / Jordan Krebs / Subhash Tripathi / Noboru Sakabe / Débora R Sobreira / Su-Chen Huang / Suhas S P Rao / Nathanael Pruett / Daniel Chauss / Erica Sadler / Andrea Lopez / Marcelo A Nóbrega / Erez Lieberman Aiden / Francisco J Asturias / Rafael Casellas /
要旨: While Mediator plays a key role in eukaryotic transcription, little is known about its mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays, Hi-C, and cryoelectron ...While Mediator plays a key role in eukaryotic transcription, little is known about its mechanism of action. This study combines CRISPR-Cas9 genetic screens, degron assays, Hi-C, and cryoelectron microscopy (cryo-EM) to dissect the function and structure of mammalian Mediator (mMED). Deletion analyses in B, T, and embryonic stem cells (ESC) identified a core of essential subunits required for Pol II recruitment genome-wide. Conversely, loss of non-essential subunits mostly affects promoters linked to multiple enhancers. Contrary to current models, however, mMED and Pol II are dispensable to physically tether regulatory DNA, a topological activity requiring architectural proteins. Cryo-EM analysis revealed a conserved core, with non-essential subunits increasing structural complexity of the tail module, a primary transcription factor target. Changes in tail structure markedly increase Pol II and kinase module interactions. We propose that Mediator's structural pliability enables it to integrate and transmit regulatory signals and act as a functional, rather than an architectural bridge, between promoters and enhancers.
履歴
登録2020年3月4日-
ヘッダ(付随情報) 公開2021年3月10日-
マップ公開2021年3月10日-
更新2024年10月23日-
現状2024年10月23日処理サイト: RCSB / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.1
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.1
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-6w1s
  • 表面レベル: 0.1
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_21514.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_21514.map.gz / 形式: CCP4 / 大きさ: 669.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Mammalian Mediator complex
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 560 pix.
= 621.6 Å		1.11 Å/pix.
x 560 pix.
= 621.6 Å		1.11 Å/pix.
x 560 pix.
= 621.6 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 1.11 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 0.1 / ムービー #1: 0.1
最小 - 最大-0.39771584 - 0.66880524
平均 (標準偏差)0.00015942252 (±0.021543505)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ560560560
Spacing560560560
セルA=B=C: 621.60004 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.111.111.11
M x/y/z560560560
origin x/y/z0.0000.0000.000
length x/y/z621.600621.600621.600
α/β/γ90.00090.00090.000
start NX/NY/NZ-170-170-170
NX/NY/NZ340340340
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS560560560
D min/max/mean-0.3980.6690.000

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添付データ

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追加マップ: Mammalian Mediator complex

ファイルemd_21514_additional_1.map
注釈Mammalian Mediator complex
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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追加マップ: Mammalian Mediator complex

ファイルemd_21514_additional_2.map
注釈Mammalian Mediator complex
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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試料の構成要素

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全体 : Mouse mediator complex

全体名称: Mouse mediator complex
要素
  • 複合体: Mouse mediator complex
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 1
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 4
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 6
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 7
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 8
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 9
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 10
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 11
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 14
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 15
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 16
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 17
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 18
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 19
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 20
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 21
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 22
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 23
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 24
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 25
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 27
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 28
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 29
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 30
    • タンパク質・ペプチド: Mediator of RNA polymerase II transcription subunit 31

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超分子 #1: Mouse mediator complex

超分子名称: Mouse mediator complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 1.0 MDa

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分子 #1: Mediator of RNA polymerase II transcription subunit 1

分子名称: Mediator of RNA polymerase II transcription subunit 1
タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 32.527998 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #2: Mediator of RNA polymerase II transcription subunit 4

分子名称: Mediator of RNA polymerase II transcription subunit 4
タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 11.647339 KDa
配列文字列:
LLSALEDLEV LSRELIEMLA ISRNQKLLQL EEENQVLELL IHRDGDFQEL MKLALNQGKV HHEMQALEKE VEKRDSDIQQ LQKQLKEAE QILATAVYQA K

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

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分子 #3: Mediator of RNA polymerase II transcription subunit 6

分子名称: Mediator of RNA polymerase II transcription subunit 6
タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 28.473553 KDa
配列文字列: MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP ...文字列:
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP SSIFQRQRVD ALLIDLRQKF PPRFVQQKSG EKPVPVDQAK KEAEPLPETV KSEEKESTKN IQQTVSTKGP PE KRMRLQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

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分子 #4: Mediator of RNA polymerase II transcription subunit 7

分子名称: Mediator of RNA polymerase II transcription subunit 7
タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 18.836996 KDa
配列文字列:
PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER VIEMIQNC

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

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分子 #5: Mediator of RNA polymerase II transcription subunit 8

分子名称: Mediator of RNA polymerase II transcription subunit 8
タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 20.156684 KDa
配列文字列:
QLEASLDALL NQVADLKNSL GSFIYKLENE YDRLTWPSVL DSFALLSGQL NTLNKVLKHE KTPLFRNQVI IPLVLSPDRD EDLMRQTEG RVPVFSHEVV PDHLRTKPDP EVEEQEKQLT TDAARIGADA AQKQIQSLNK MCSNLLEKIS KEERESESGG L RPNKQTFN PGDTNALVAA VA

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

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分子 #6: Mediator of RNA polymerase II transcription subunit 9

分子名称: Mediator of RNA polymerase II transcription subunit 9
タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 8.924443 KDa
配列文字列:
FLPLVHNVIK CMDKDSPDLH QDLNALKTKF QELRKLIGTM PGIHVSPEQQ QQQLHSLREQ VRTKNELLQK YKSLCM

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

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分子 #7: Mediator of RNA polymerase II transcription subunit 10

分子名称: Mediator of RNA polymerase II transcription subunit 10
タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 15.951878 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)IDKCRQ QLHDITVPLE VFEYIDQGRN PQLYTKECLE RALAKNEQVK GKID TMKKF KSLLIQELSK VFPEDMAKYR SIRGEDHPPS

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

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分子 #8: Mediator of RNA polymerase II transcription subunit 11

分子名称: Mediator of RNA polymerase II transcription subunit 11
タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 12.166723 KDa
配列文字列:
NERLRALEDI EREIGAILQN AGTAILELSK EKTNERLLDR QAAAFTTSVQ HVEAELSAQI RYLTQVATGQ PHEGSSYSSR KDCQMALKR VDYARLKISD VARTCEQML

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

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分子 #9: Mediator of RNA polymerase II transcription subunit 14

分子名称: Mediator of RNA polymerase II transcription subunit 14
タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 168.719531 KDa
配列文字列: MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT ...文字列:
MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT TGSYPRLPTC IRDKIIPPDP ITKIEKQATL HQLNQILRHR LVTTDLPPQL ANLTVANGRV KFRVEGEFEA TL TVMGDDP EVPWRLLKLE ILVEDKETGD GRALVHSMQI DFIHQLVQSR LFADEKPLQD MYNCLHCFCL SLQLEVLHSQ TLM LIRERW GDLVQVERYH AGKSLSLSVW NQQVLGRKTG TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDH LSIEK LLIDSVHARA HQRLQELKAI LRSFNANESS SIETALPALI VPILEPCGNS ECLHIFVDLH SGMFQLMLYG LDPAT LEDM EKSLNDDMKR IIPWIQQLKF WLGQQRCKQS IKHLPTITTE TLQLANYSTH PIGSLSKNKL FIKLTRLPQY YIVVEM LEV PNKPTQLSYN YYFMSVSTAD REDSPVMALL LQQFKDNIQD LMSYTKTGKQ TRTGTKHKLS DDPCPIDSKK AKRSGEM CA FNKVLAHFVA MCDTNMPFVG LRLELSNLEI PHQGVQVEGD GFNHAIRLLK IPPCKGISEE TQKALDRSLL DCTFRLQG R NNRTWVAELV FANCPLNGTS TREQGPSRHV YLTYENLLSE PVGGRKVVEM FLNDWSSIAR LYECVLEFAR SLPEIPAHL NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAIN KLPTVPMLGL TQRTNTAYQC FSILPQSSTH IRLAFRNMYC IDIYCRSRGV VAIRDGAYSL FDNSKLVEGF Y PAPGLKTF LNMFVDSNQD ARRRSVNEDD NPPSPIGGDM MDSLISQLQP PQQQPFPKQP GTSGAYPLTS PPTSYHSTVN QS PSMMHTQ SPGNLHAASS PSGALRAPSP ASFVPTPPPS SHGISIGPGA SFASPHGTLD PSSPYTMVSP SGRAGNWPGS PQV SGPSPA TRLPGMSPAN PSLHSPVPDV SHSPRAGTSS QTMPTNMPPP RKLPQRSWAA SIPTILTHSA LNILLLPSPT PGLV PGLAG SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR VALSPKTNQT LQLKVTPENA GQWKP DELQ VLEKFFETRV AGPPFKANTL IAFTKLLGAP THILRDCVHI MKLELFPDQA TQLKWNVQFC LTIPPSAPPI APPGTP AVV LKSKMLFFLQ LTQKTSVPPQ EPVSIIVPII YDMASGTTQQ ADIPRQQNSS VAAPMMVSNI LKRFAEMNPP RQGECTI FA AVRDLMANLT LPPGGRP(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

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分子 #10: Mediator of RNA polymerase II transcription subunit 15

分子名称: Mediator of RNA polymerase II transcription subunit 15
タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 18.904717 KDa
配列文字列:
QPLLDAVLAN IRSPVFNHSL YRTFVPAMMA IHGPPIVSPV VCSRKRRFEE DERQSIPNVL QGEVARLDPK FLVNLDPSHC SNNGTVHLI CKLDDKDLPS VPPLELSVPA DYPAQSPMWI DRQWQYDANP FLQSVHRCMT SRLLQLPDKH SVTALLNTWA Q SIHQACL

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

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分子 #11: Mediator of RNA polymerase II transcription subunit 16

分子名称: Mediator of RNA polymerase II transcription subunit 16
タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 91.890484 KDa
配列文字列: MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI ...文字列:
MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAA ADGSSASPVK FYKVCVSVVS EK CRIDTEI LPSLFMRCTT DPNRKDRFPA ITHLKFLARD MSEQVLLCAS SQTSSLVECW SLRKEGLPVN NIFQQISPVV GDK QPMILK WRILSATNDL DRVSAVALPK LPISLTNTDL KVASDTQFYP GLGLALAFQD GSVHMVHRLS LQTMAVFYSS APRS LDEPA LKRPRTTCPA VHFKAMQLSW TSLALVGIDN HGKLSMLRIS PSLGHPLEPK LALQHLLFLL EYCMVTGYDW WDILL HVQP GMVQSLVERL HEEYTRQKPA LQQVLSTRIL AMKASLCKLS PCTVARVCDY HTKLFLMAIT STLKSLLRPH FLNTPD KSP GDRLAEICAK ITDVDIDKVM INLKTEEFVL DMNTLQALQQ LLQWVGDFVL YLLVSLPNQG SPLRPGHSFL RDGTSLG ML RELMVVIRIW GLLKPSCLPV YTATSDTQDS MSLLFRLLTK LWICCRDEGA ASEPDEGLVD ECCLLPSQLL VPNLDWLP A SDGLVSRLQP KQPLRLRFGR APTLPSSTST LQLDGLTRAP GQPKIDHLRR LHLGAYPTEE CKACTRCGCV TMLKSPNKT TAVTQWEQRW IKNCLCGGLW RRVPLSCS

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

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分子 #12: Mediator of RNA polymerase II transcription subunit 17

分子名称: Mediator of RNA polymerase II transcription subunit 17
タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 70.66932 KDa
配列文字列: CEKQVQEVGL DGTETYLQPL SMSQNLARLA QRIDFSQGSG SEEEEAAGPD GDAPDWGGAG ADQDDEEGLV KFQPSLWPWD SVRNNLRSA LTEMCVLYDV LSIVRDKKFM TLDPVSQDAL PPKQSPQTLQ LISKKKSLAG AAQILLKGAE RLTKSVAENQ E NKLQRDFN ...文字列:
CEKQVQEVGL DGTETYLQPL SMSQNLARLA QRIDFSQGSG SEEEEAAGPD GDAPDWGGAG ADQDDEEGLV KFQPSLWPWD SVRNNLRSA LTEMCVLYDV LSIVRDKKFM TLDPVSQDAL PPKQSPQTLQ LISKKKSLAG AAQILLKGAE RLTKSVAENQ E NKLQRDFN SELLRLRQHW KLRKVGDKIL GDLSYRSAGS LFPHHGTFEV IKNTDIDLDK KIPEDYCPLD VQIPSDLEGS AY IKVSIQK QAPDIGDLGT VNLFKRPLPK SKPGSPHWQT KLEAAQNVLL CKEIFAQLSR EAVQIKSQIP HIVVKNQIIS QPF PSLQLS ISLCHSSDDK KSQKCAAEKP GQEDHLYVLE HNLHLLIREF HKQTLSSIVM PHPASAPFGH KRMRLSGPQA FDKN EINSI QSTEGLLEKI IKQAKHIFLR SRTAATIDSL ASRIEDPQIQ AHWSNINDVY ESSVKVLITS QGYEQICKSI QLQLN IGVE QVRVVHRDGR VIMLSHQEQE LQDFLLSQMS QHQVHAVQQL AKVMGWQVLS FSNHVGLGPI ESIGNASAIT VASPSG DYA ISVRNGPESG SKIMVQFPRN QCKDLPKSDV LQDSKWSHLR GPFKEVQWNK MEGRNFVYKM ELLMSALS

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

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分子 #13: Mediator of RNA polymerase II transcription subunit 18

分子名称: Mediator of RNA polymerase II transcription subunit 18
タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 21.43385 KDa
配列文字列:
NMMEYLLQGS VLDHSLESLI HRLRGLCDNM EPETFLDHEM VFLLKGQQAS PFVLRARRSM DRAGAPWHLR YLGQPEMGDK NRHALVRNC VDIATSENLT DFLMEMGFRM DHEFVAKGHL FRKGIMKVVV YKIFRILVPG NTDSTEALSL SYLVELSVVA P AGQDMVSD DMRNFAEQLK PLVHLEKID

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

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分子 #14: Mediator of RNA polymerase II transcription subunit 19

分子名称: Mediator of RNA polymerase II transcription subunit 19
タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 24.684615 KDa
配列文字列: MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTEL (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTEL (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)L GGSFNPITGT MLSGFRLHTG PLPEQCRLM HIQPPKKKNK HKHKQSRTQD PVPPETPSDS DHKKKKKKKE EDPERKRKKK EKKKKKNRHS PDHPGMGSSQ ASSSSSLR

UniProtKB: Mediator of RNA polymerase II transcription subunit 19, Mediator of RNA polymerase II transcription subunit 19

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分子 #15: Mediator of RNA polymerase II transcription subunit 20

分子名称: Mediator of RNA polymerase II transcription subunit 20
タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 21.660906 KDa
配列文字列: VTCVSQMPVA EGKSLQQTVE LLTKKLEMLG AEKQGTFCVD CETYHTAAST LGSQGQAGKL MYVMHNSEYP LSCFALFENG PCLIADTNF DVLMVKLKGF FQSAKASKIE TRGTRYQYCD FLVKVGTVTM GPSARGISVE VEYGPCVVAS DCWSLLLEFL Q SFLGSHAP ...文字列:
VTCVSQMPVA EGKSLQQTVE LLTKKLEMLG AEKQGTFCVD CETYHTAAST LGSQGQAGKL MYVMHNSEYP LSCFALFENG PCLIADTNF DVLMVKLKGF FQSAKASKIE TRGTRYQYCD FLVKVGTVTM GPSARGISVE VEYGPCVVAS DCWSLLLEFL Q SFLGSHAP GAPTVFGNRH DAVYGPADTM IQYMELFNKI

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

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分子 #16: Mediator of RNA polymerase II transcription subunit 21

分子名称: Mediator of RNA polymerase II transcription subunit 21
タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 13.619062 KDa
配列文字列:
DRLTQLQDAV NSLADQFCNA IGVLQQCGPP ASFSNIQTAI NKDQPANPTE EYAQLFAALI ARTAKDIDVL IDSLPSEEST AALQAASLY KLEEENHEAA TCLEDVVYRG DMLLEKIQSA LADIAQS

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

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分子 #17: Mediator of RNA polymerase II transcription subunit 22

分子名称: Mediator of RNA polymerase II transcription subunit 22
タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 15.284022 KDa
配列文字列:
QSKETLLQSY NKRLKDDIKS IMDNFTEIIK TAKIEDETQV SRATQGEQDN YEMHVRAANI VRAGESLMKL VSDLKQFLIL NDFPSVNEA IDQRNQQLRA LQEECDRKLI TLRDEVSIDL YELEEEYYSS SS

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

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分子 #18: Mediator of RNA polymerase II transcription subunit 23

分子名称: Mediator of RNA polymerase II transcription subunit 23
タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 156.265656 KDa
配列文字列: METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA ...文字列:
METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KECIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNGDSTLQI PIPHSLKLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRVPLPGTSC LASASVTPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHTK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSHRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLNDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM N FTPHNWASHT LSCFPAPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMT DENEIITQFS VQGFPPLFLC LLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQSDWHT KHMSYHKKYP EKLYFEGLAE QVDPPVPIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMCLRN RDHLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKHAMNARE DNPWVPEDSY YCKLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV PGK DVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVNVISSSS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEAL LPVVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAVPP QALNSGSPAP QSNQVP ASL PVTQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 23

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分子 #19: Mediator of RNA polymerase II transcription subunit 24

分子名称: Mediator of RNA polymerase II transcription subunit 24
タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 109.563117 KDa
配列文字列: VNLKQAILQA WKERWSDYQW AINMKKFFPK GATWDILNLA EALLEQAMIG PSPNPLILSY LKYAISSQMV SCSSVLTAIS KFDDFSRDL CVQALLDIMD MFCDRLSCHG KAEECIGLCR ALLSALHWLL RCTAASAERL QEGLEAGTPA PGEKQLALCL Q CLEKTLSS ...文字列:
VNLKQAILQA WKERWSDYQW AINMKKFFPK GATWDILNLA EALLEQAMIG PSPNPLILSY LKYAISSQMV SCSSVLTAIS KFDDFSRDL CVQALLDIMD MFCDRLSCHG KAEECIGLCR ALLSALHWLL RCTAASAERL QEGLEAGTPA PGEKQLALCL Q CLEKTLSS TKNRALLHIA KLEEASSWTA IEHSLLKLGE ILANLSNPQL RSQAERCGTL IRSIPSMLSV HSEQLHKTGF PT IHALILL EGTMNLTGEM QPLVEQLMMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT QELKWTAFTY LKIPQVLVKL KKY FHGEKD FTEDVNCAFE FLLKLTPLLD KADQRCNCDC TNFLLQECNK QGLLSEVNFA SLVGKRTADR DPQLKSSENA NIQP NPGLI LRAEPTVTNI LKTMDADHSK SPEGLLGVLG HMLSGKSLDL LLAAAAATGK LKSFARKFIN LNEFTTHGSG ESTKT ASVR ALLFDISFLM LCHVAQTYGS EVILSESSSG EEVPFFETWM QTCMPEEGKI LNPDHPCFRP DSTKVESLVA LLNNSS EMK LVQMKWHEAC LSISAAILEI LNAWENGVLA FESIQKITDN IKGKVCSLAV CAVAWLVAHV RMLGLDEREK SLQMIRQ LA GPLYSENTLQ FYNERVVIMN SILEHMCADV LQQTATQIKF PSTGVDTMPY WNLLPPKRPI KEVLTDIFAK VLEKGWVD S RSIHILDTLL HMGGVYWFCN NLIKELLKET RKEHTLRAVQ LLYSIFCLDM QQVTLVLLGH ILPGLLTDSS KWHSLMDPP GTALAKLAVW CALSSYSSHK GQASSRQKKR HREDIEDYVS LFPVEDMQPS KLMRLLSSSD DDANILSSPT DRSMNSSLSA SQLHTVNMR DPLNRVLANL FLLISSILGS RTAGPHTQFV QWFMEECVGC LEQDSRGSIL QFMPFTTVSE LVKVSAMSSP K VVLAITDL SLPLGRQVAA KAIA

UniProtKB: Mediator of RNA polymerase II transcription subunit 24

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分子 #20: Mediator of RNA polymerase II transcription subunit 25

分子名称: Mediator of RNA polymerase II transcription subunit 25
タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 22.032941 KDa
配列文字列: VADVVFVIEG TANLGPYFEE LRKHYLLPAI EYFNGGPPAE TDFGGDYGGT QYSLVVFNTV DCAPESYVQC HAPTSSAYEF VTWLDGIKF MGGGGESCSL IAEGLSTALQ LFDDFKKMRE QIGQTHRVCL LICNSPPYLL PAVESTTYSG CTTESLVQKI G ERGIHFSI ...文字列:
VADVVFVIEG TANLGPYFEE LRKHYLLPAI EYFNGGPPAE TDFGGDYGGT QYSLVVFNTV DCAPESYVQC HAPTSSAYEF VTWLDGIKF MGGGGESCSL IAEGLSTALQ LFDDFKKMRE QIGQTHRVCL LICNSPPYLL PAVESTTYSG CTTESLVQKI G ERGIHFSI VSPRKLPALR LLFEKAAPPA LLEPLQQPAD VSQD

UniProtKB: Mediator of RNA polymerase II transcription subunit 25

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分子 #21: Mediator of RNA polymerase II transcription subunit 27

分子名称: Mediator of RNA polymerase II transcription subunit 27
タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 33.737773 KDa
配列文字列: GVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIANFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDP VQDKTPLYSQ LLQAYKWSNK LQYHAGLASG LLNQQSLKRS ANQMGVSAKR RPKAQPTTLV LPPQYVDDVI S RIDRMFPE ...文字列:
GVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIANFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDP VQDKTPLYSQ LLQAYKWSNK LQYHAGLASG LLNQQSLKRS ANQMGVSAKR RPKAQPTTLV LPPQYVDDVI S RIDRMFPE MSIHLSRPNG TSAMLLVTLG KVLKVIVVMR SLFIDRTIVK GYNESVYTED GKLDIWSKSS YQVFQKVTDH AT TALLHYQ LPQMPDVVVR SFMTWLRSYI KLFQAPCQRC GKFLQDGLPP TWRDFRTLEA

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

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分子 #22: Mediator of RNA polymerase II transcription subunit 28

分子名称: Mediator of RNA polymerase II transcription subunit 28
タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 13.531168 KDa
配列文字列:
AAPGAPRPSN STLVDELESS FEACFASLVS QDYVNGTDQE EIRTGVDQCI QKFLDIARQT ECFFLQKRLQ LSVQKPDQVI KEDVSELRS ELQRKDALVQ KHLTKLRHWQ QVLEDINVQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 28

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分子 #23: Mediator of RNA polymerase II transcription subunit 29

分子名称: Mediator of RNA polymerase II transcription subunit 29
タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 14.965154 KDa
配列文字列:
DFDPVQRYKM LIPQLKESLQ TLMKVAAQNL IQNTNIDNGQ KSSDAPLQRF DKCLEEFYAL CDQLELCLRL AHECLSQSCD SAKHSPTLV PTATKPDAVQ PDSLPYPQYL AVIKAQITCA KDIHTALLDC ANKVT

UniProtKB: Mediator of RNA polymerase II transcription subunit 29

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分子 #24: Mediator of RNA polymerase II transcription subunit 30

分子名称: Mediator of RNA polymerase II transcription subunit 30
タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 17.873512 KDa
配列文字列:
EVNTATLCRI GQETVQDIVY RTMEIFQLLR NMQLPNGVTY HTGTYQDRLT KLQDHLRQLS ILFRKLRLVY DKCNENCGGM DPIPVEQLI PYVDEDGSKN DDRAGPPRFA SEERREIVEV NKKLKQKNQQ LKQIMDQLRN LIWDINAMLA MRN

UniProtKB: Mediator of RNA polymerase II transcription subunit 30

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分子 #25: Mediator of RNA polymerase II transcription subunit 31

分子名称: Mediator of RNA polymerase II transcription subunit 31
タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Mus musculus (ハツカネズミ)
分子量理論値: 11.54537 KDa
配列文字列:
GNRLRFQLEL EFVQCLANPN YLNFLAQRGY FKDKAFVNYL KYLLYWKEPE YAKYLKYPQC LHMLELLQYE HFRKELVNAQ CAKFIDEQQ ILHW

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

濃度0.1 mg/mL
緩衝液pH: 7.9
糖包埋材質: ice
グリッドモデル: PELCO Ultrathin Carbon with Lacey Carbon / 材質: COPPER / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 30 / 前処理 - タイプ: PLASMA CLEANING / 前処理 - 時間: 6 sec.
凍結凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: HOMEMADE PLUNGER

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電子顕微鏡法

顕微鏡FEI TALOS ARCTICA
撮影フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 22.5 e/Å2
電子線加速電圧: 200 kV / 電子線源: FIELD EMISSION GUN
電子光学系C2レンズ絞り径: 50.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm
実験機器
モデル: Talos Arctica / 画像提供: FEI Company

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画像解析

初期モデルモデルのタイプ: INSILICO MODEL
最終 再構成解像度のタイプ: BY AUTHOR / 解像度: 4.02 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 217557
初期 角度割当タイプ: MAXIMUM LIKELIHOOD
最終 角度割当タイプ: MAXIMUM LIKELIHOOD
FSC曲線 (解像度の算出)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る