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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-21000 | ||||||||||||||||||
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タイトル | Lipophilic Envelope-spanning Tunnel B (LetB), Map 8 | ||||||||||||||||||
![]() | Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8 (rings 1 to 3 in open state, after masking and signal subtraction in Relion) | ||||||||||||||||||
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![]() | conformational dynamics / lipid transport / bacterial cell envelope / LetB / YebT / MCE | ||||||||||||||||||
機能・相同性 | : / Mce/MlaD / MlaD protein / intermembrane lipid transfer / outer membrane-bounded periplasmic space / identical protein binding / plasma membrane / Intermembrane transport protein YebT![]() | ||||||||||||||||||
生物種 | ![]() ![]() ![]() ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
![]() | Isom GL / Coudray N | ||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope. 著者: Georgia L Isom / Nicolas Coudray / Mark R MacRae / Collin T McManus / Damian C Ekiert / Gira Bhabha / ![]() 要旨: Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate ...Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 Å cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel sufficiently long to span the periplasm. Lipids bind inside the tunnel, suggesting that it functions as a pathway for lipid transport. Cryo-EM structures in the open and closed states reveal a dynamic tunnel lining, with implications for gating or substrate translocation. Our results support a model in which LetB establishes a physical link between the two membranes and creates a hydrophobic pathway for the translocation of lipids across the periplasm. | ||||||||||||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 49.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 13.6 KB 13.6 KB | 表示 表示 | ![]() |
画像 | ![]() | 101.4 KB | ||
Filedesc metadata | ![]() | 6 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 6v0jMC ![]() 6v0cC ![]() 6v0dC ![]() 6v0eC ![]() 6v0fC ![]() 6v0gC ![]() 6v0hC ![]() 6v0iC ![]() 6vciC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data size: 928.6 Data #1: Aligned, dose-weighted and non-dose-weighted micrographs [micrographs - single frame] Data #2: Particle stacks for Model 1 (EMD-20993) [picked particles - single frame - processed] Data #3: Particle stacks for Model 2 (EMD-20994) [picked particles - single frame - processed] Data #4: Particle stacks for Model 3 (EMD-20995) [picked particles - single frame - processed] Data #5: Particle stacks for Model 4 (EMD-20996) [picked particles - single frame - processed] Data #6: Particle stacks for Model 5 (EMD-20997) [picked particles - single frame - processed] Data #7: Particle stacks for Model 6 (EMD-20998) [picked particles - single frame - processed] Data #8: Particle stacks for Model 7 (EMD-20999) [picked particles - single frame - processed] Data #9: Particle stacks for Model 8 (EMD-21000) [picked particles - single frame - processed]) |
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リンク
EMDBのページ | ![]() ![]() |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8 (rings 1 to 3 in open state, after masking and signal subtraction in Relion) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.31 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8
全体 | 名称: Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8 |
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要素 |
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-超分子 #1: Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8
超分子 | 名称: Lipophilic Envelope-spanning Tunnel B (LetB), Map 8, Model 8 タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all / 詳細: Cryo-EM reconstruction of rings 1 to 3, class 1.1.3 |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 560 KDa |
-分子 #1: Intermembrane transport protein YebT
分子 | 名称: Intermembrane transport protein YebT / タイプ: protein_or_peptide / ID: 1 / コピー数: 6 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 89.744031 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: GNTVTIDFMS ADGIVPGRTP VRYQGVEVGT VQDISLSDDL RKIEVKVSIK SDMKDALREE TQFWLVTPKA SLAGVSGLDA LVGGNYIGM MPGKGKEQDH FVALDTQPKY RLDNGDLMIH LQAPDLGSLN SGSLVYFRKI PVGKVYDYAI NPNKQGVVID V LIERRFTD ...文字列: GNTVTIDFMS ADGIVPGRTP VRYQGVEVGT VQDISLSDDL RKIEVKVSIK SDMKDALREE TQFWLVTPKA SLAGVSGLDA LVGGNYIGM MPGKGKEQDH FVALDTQPKY RLDNGDLMIH LQAPDLGSLN SGSLVYFRKI PVGKVYDYAI NPNKQGVVID V LIERRFTD LVKKGSRFWN VSGVDANVSI SGAKVKLESL AALVNGAIAF DSPEESKPAE AEDTFGLYED LAHSQRGVII KL ELPSGAG LTADSTPLMY QGLEVGQLTK LDLNPGGKVT GEMTVDPSVV TLLRENTRIE LRNPKLSLSD ANLSALLTGK TFE LVPGDG EPRKEFVVVP GEKALLHEPD VLTLTLTAPE SYGIDAGQPL ILHGVQVGQV IDRKLTSKGV TFTVAIEPQH RELV KGDSK FVVNSRVDVK VGLDGVEFLG ASASEWINGG IRILPGDKGE MKASYPLYAN LEKALENSLS DLPTTTVSLS AETLP DVQA GSVVLYRKFE VGEVITVRPR ANAFDIDLHI KPEYRNLLTS NSVFWAEGGA KVQLNGSGLT VQASPLSRAL KGAISF DNL SGASASQRKG DKRILYASET AARAVGGQIT LHAFDAGKLA VGMPIRYLGI DIGQIQTLDL ITARNEVQAK AVLYPEY VQ TFARGGTRFS VVTPQISAAG VEHLDTILQP YINVEPGRGN PRRDFELQEA TITDSRYLDG LSIIVEAPEA GSLGIGTP V LFRGLEVGTV TGMTLGTLSD RVMIAMRISK RYQHLVRNNS VFWLASGYSL DFGLTGGVVK TGTFNQFIRG GIAFATPPG TPLAPKAQEG KHFLLQESEP KEWREWGTAL PK UniProtKB: Intermembrane transport protein YebT |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.5 mg/mL |
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緩衝液 | pH: 8 |
凍結 | 凍結剤: ETHANE / 装置: FEI VITROBOT MARK III |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) デジタル化 - 画像ごとのフレーム数: 1-50 / 撮影したグリッド数: 1 / 実像数: 4906 / 平均電子線量: 80.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |