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- EMDB-20648: CryoEM Structure of Pyocin R2 - postcontracted - baseplate -

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Basic information

Entry
Database: EMDB / ID: EMD-20648
TitleCryoEM Structure of Pyocin R2 - postcontracted - baseplate
Map dataStructure of Pyocin R2 - postcontracted - baseplate
Sample
  • Complex: Pyocin R2 - Postcontracted
    • Protein or peptide: Sheath PA0622
    • Protein or peptide: Glue PA0627
    • Protein or peptide: Tri1a PA0618
    • Protein or peptide: Tri2 PA0619
    • Protein or peptide: Sheath Initiator PA0617
Keywordsbacteriocin / pyocin / ANTIMICROBIAL PROTEIN
Function / homology
Function and homology information


Phage Tail Protein X-like / Baseplate assembly protein J, predicted / Phage Tail Protein X / Tail protein I / Phage tail protein (Tail_P2_I) / : / : / Tail sheath protein Gp18 domain III N-terminal region / IraD/Gp25-like / Baseplate wedge protein gp25 ...Phage Tail Protein X-like / Baseplate assembly protein J, predicted / Phage Tail Protein X / Tail protein I / Phage tail protein (Tail_P2_I) / : / : / Tail sheath protein Gp18 domain III N-terminal region / IraD/Gp25-like / Baseplate wedge protein gp25 / Baseplate protein J-like / Baseplate J-like protein barrel domain / : / Tail sheath protein, subtilisin-like domain / Phage tail sheath protein subtilisin-like domain / Tail sheath protein, C-terminal domain / Phage tail sheath C-terminal domain
Similarity search - Domain/homology
Probable bacteriophage protein / Probable bacteriophage protein / Probable bacteriophage protein / Phage tail protein / Probable bacteriophage protein
Similarity search - Component
Biological speciesPseudomonas aeruginosa PAO1 (bacteria) / Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsGe P / Avaylon J
Funding support United States, Switzerland, 4 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM071940 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R21AI085318 United States
Swiss National Science Foundation31003A_146284 Switzerland
National Science Foundation (NSF, United States)XSEDE MCB140140 United States
CitationJournal: Nature / Year: 2020
Title: Action of a minimal contractile bactericidal nanomachine.
Authors: Peng Ge / Dean Scholl / Nikolai S Prokhorov / Jaycob Avaylon / Mikhail M Shneider / Christopher Browning / Sergey A Buth / Michel Plattner / Urmi Chakraborty / Ke Ding / Petr G Leiman / Jeff ...Authors: Peng Ge / Dean Scholl / Nikolai S Prokhorov / Jaycob Avaylon / Mikhail M Shneider / Christopher Browning / Sergey A Buth / Michel Plattner / Urmi Chakraborty / Ke Ding / Petr G Leiman / Jeff F Miller / Z Hong Zhou /
Abstract: R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded ...R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
History
DepositionAug 27, 2019-
Header (metadata) releaseOct 2, 2019-
Map releaseApr 15, 2020-
UpdateMar 20, 2024-
Current statusMar 20, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6u5k
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_20648.png

Downloads & links

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Map

FileDownload / File: emd_20648.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationStructure of Pyocin R2 - postcontracted - baseplate
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.04 Å/pix.
x 420 pix.
= 437.22 Å		1.04 Å/pix.
x 420 pix.
= 437.22 Å		1.04 Å/pix.
x 420 pix.
= 437.22 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.041 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.107300244 - 0.18900001
Average (Standard dev.)0.000013670184 (±0.010422632)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-210-210-210
Dimensions420420420
Spacing420420420
CellA=B=C: 437.22 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0411.0411.041
M x/y/z420420420
origin x/y/z0.0000.0000.000
length x/y/z437.220437.220437.220
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ460460460
MAP C/R/S123
start NC/NR/NS-210-210-210
NC/NR/NS420420420
D min/max/mean-0.1070.1890.000

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Supplemental data

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Sample components

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Entire : Pyocin R2 - Postcontracted

EntireName: Pyocin R2 - Postcontracted
Components
  • Complex: Pyocin R2 - Postcontracted
    • Protein or peptide: Sheath PA0622
    • Protein or peptide: Glue PA0627
    • Protein or peptide: Tri1a PA0618
    • Protein or peptide: Tri2 PA0619
    • Protein or peptide: Sheath Initiator PA0617

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Supramolecule #1: Pyocin R2 - Postcontracted

SupramoleculeName: Pyocin R2 - Postcontracted / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Pseudomonas aeruginosa PAO1 (bacteria)

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Macromolecule #1: Sheath PA0622

MacromoleculeName: Sheath PA0622 / type: protein_or_peptide / ID: 1 / Number of copies: 24 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 41.247332 KDa
SequenceString: MSFFHGVTVT NVDIGARTIA LPASSVIGLC DVFTPGAQAS AKPNVPVLLT SKKDAAAAFG IGSSIYLACE AIYNRAQAVI VAVGVETAE TPEAQASAVI GGISAAGERT GLQALLDGKS RFNAQPRLLV APGHSAQQAV ATAMDGLAEK LRAIAILDGP N STDEAAVA ...String:
MSFFHGVTVT NVDIGARTIA LPASSVIGLC DVFTPGAQAS AKPNVPVLLT SKKDAAAAFG IGSSIYLACE AIYNRAQAVI VAVGVETAE TPEAQASAVI GGISAAGERT GLQALLDGKS RFNAQPRLLV APGHSAQQAV ATAMDGLAEK LRAIAILDGP N STDEAAVA YAKNFGSKRL FMVDPGVQVW DSATNAARNA PASAYAAGLF AWTDAEYGFW SSPSNKEIKG VTGTSRPVEF LD GDETCRA NLLNNANIAT IIRDDGYRLW GNRTLSSDSK WAFVTRVRTM DLVMDAILAG HKWAVDRGIT KTYVKDVTEG LRA FMRDLK NQGAVINFEV YADPDLNSAS QLAQGKVYWN IRFTDVPPAE NPNFRVEVTD QWLTEVLDVA

UniProtKB: Probable bacteriophage protein

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Macromolecule #2: Glue PA0627

MacromoleculeName: Glue PA0627 / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 7.486475 KDa
SequenceString:
MATTCRTADG DMLDSLCYHV YGHLLGCVEA TLDANPGLAD EQQPFRAGLL ISFPDMPVVN VEQVRLWD

UniProtKB: Phage tail protein

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Macromolecule #3: Tri1a PA0618

MacromoleculeName: Tri1a PA0618 / type: protein_or_peptide / ID: 3 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 31.986117 KDa
SequenceString: MIIDLSQLPE PEVIENLDFE TIYQELLGDF REAMAGEWTA EVESDPVLKL LQLAAYRELL LRARINDAAR AVMLAYASGA DLDQIGAGF NVQRLLIRPA QPEAVPPVEA QYESDKSLRN RIQLAFEQLS VAGPRNAYIA HALGADGRVA DASATSPAPC E VLISVLGV ...String:
MIIDLSQLPE PEVIENLDFE TIYQELLGDF REAMAGEWTA EVESDPVLKL LQLAAYRELL LRARINDAAR AVMLAYASGA DLDQIGAGF NVQRLLIRPA QPEAVPPVEA QYESDKSLRN RIQLAFEQLS VAGPRNAYIA HALGADGRVA DASATSPAPC E VLISVLGV EGNGQAPEAV LQAVRLALNA EDVRPVADRV TVRSAGIVPY QVKAQLYLFP GPEAELIRAA AEASLRDYIS AQ RRLGRDI RRSALFATLH VEGVQRVELQ EPAADVVLDE TQAAYCTGYA ITLGGVDE

UniProtKB: Probable bacteriophage protein

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Macromolecule #4: Tri2 PA0619

MacromoleculeName: Tri2 PA0619 / type: protein_or_peptide / ID: 4 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 20.013682 KDa
SequenceString:
MSSRLLPPNR SSLERSLGDV LPAELPVPLR ELHDPARCEA ALLPYLAWTR SVDRWDPDWS DEAKRNAVAT SFVLHQRKGT LTALRQVVE PIGALSEVTE WWQRSPTGVP GTFEITVDVS DRGIDEGTVL ELERLLDDVR PVSRHLTRLD LRITPVIRSR H GLAVTDGD TLEIFPWKQ

UniProtKB: Probable bacteriophage protein

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Macromolecule #5: Sheath Initiator PA0617

MacromoleculeName: Sheath Initiator PA0617 / type: protein_or_peptide / ID: 5 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria)
Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
Molecular weightTheoretical: 11.862759 KDa
SequenceString:
MIGMDRRSGL PLSGLAHLKQ SVEDILTTPL GSRRMRPEYG SKLRRMVDMP VSEGWKSAVQ AEVARSLGRW EPRIGLSAVR VVAVVDGRV DLLLSGVFEG ENINMEVSA

UniProtKB: Probable bacteriophage protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
Component:
ConcentrationNameFormula
10.0 mMTris
130.0 mMSaltNaCl
GridSupport film - Material: CARBON / Support film - topology: HOLEY ARRAY / Details: unspecified
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
TemperatureMin: 80.0 K / Max: 81.0 K
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Lower energy threshold: -10 eV / Energy filter - Upper energy threshold: 10 eV
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 3-20 / Number grids imaged: 1 / Number real images: 7331 / Average exposure time: 10.0 sec. / Average electron dose: 80.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated defocus max: 3.4 µm / Calibrated defocus min: 1.1 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.16 µm / Nominal defocus min: 2.16 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 36116
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C6 (6 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 15581
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 1.4)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-6u5k:
CryoEM Structure of Pyocin R2 - postcontracted - baseplate

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