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Structure paper

TitleAction of a minimal contractile bactericidal nanomachine.
Journal, issue, pagesNature, Vol. 580, Issue 7805, Page 658-662, Year 2020
Publish dateApr 15, 2020
AuthorsPeng Ge / Dean Scholl / Nikolai S Prokhorov / Jaycob Avaylon / Mikhail M Shneider / Christopher Browning / Sergey A Buth / Michel Plattner / Urmi Chakraborty / Ke Ding / Petr G Leiman / Jeff F Miller / Z Hong Zhou /
PubMed AbstractR-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded ...R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
External linksNature / PubMed:32350467 / PubMed Central
MethodsEM (helical sym.) / EM (single particle) / X-ray diffraction
Resolution2.102 - 4.0 Å
Structure data

20526

6pyt

EMDB-20526, PDB-6pyt:
CryoEM Structure of Pyocin R2 - precontracted - trunk
Method: EM (helical sym.) / Resolution: 2.9 Å

20643

6u5b

EMDB-20643, PDB-6u5b:
CryoEM Structure of Pyocin R2 - precontracted - baseplate
Method: EM (single particle) / Resolution: 3.5 Å

20644

6u5f

EMDB-20644, PDB-6u5f:
CryoEM Structure of Pyocin R2 - precontracted - collar
Method: EM (single particle) / Resolution: 3.8 Å

20646

6u5h

EMDB-20646, PDB-6u5h:
CryoEM Structure of Pyocin R2 - precontracted - hub
Method: EM (single particle) / Resolution: 4.0 Å

20647

6u5j

EMDB-20647, PDB-6u5j:
CryoEM Structure of Pyocin R2 - postcontracted - collar
Method: EM (single particle) / Resolution: 3.5 Å

20648

6u5k

EMDB-20648, PDB-6u5k:
CryoEM Structure of Pyocin R2 - postcontracted - baseplate
Method: EM (single particle) / Resolution: 3.5 Å

PDB-5ces:
C-terminal domain of the R-type pyocin baseplate protein PA0618
Method: X-RAY DIFFRACTION / Resolution: 2.102 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • pseudomonas aeruginosa pao1 (bacteria)
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
KeywordsSTRUCTURAL PROTEIN / gpJ / gp6 / ANTIMICROBIAL PROTEIN / bacteriocin / pyocin / helix / VIRAL PROTEIN / UNKNOWN FUNCTION

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