axonemal central pair / axonemal doublet microtubule / positive regulation of cilium-dependent cell motility / outer dynein arm / outer dynein arm assembly / cilium-dependent cell motility / establishment of protein localization to organelle / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / cilium movement ...axonemal central pair / axonemal doublet microtubule / positive regulation of cilium-dependent cell motility / outer dynein arm / outer dynein arm assembly / cilium-dependent cell motility / establishment of protein localization to organelle / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / cilium movement / axoneme assembly / axonemal microtubule / negative regulation of microtubule depolymerization / nucleoside-diphosphate kinase / UTP biosynthetic process / CTP biosynthetic process / microtubule associated complex / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / mitotic cytokinesis / axoneme / cilium assembly / alpha-tubulin binding / microtubule-based process / Hsp70 protein binding / mitotic spindle organization / meiotic cell cycle / Hsp90 protein binding / ATP-dependent protein folding chaperone / structural constituent of cytoskeleton / mitotic spindle / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / microtubule / cytoskeleton / hydrolase activity / calmodulin binding / ciliary basal body / cilium / GTPase activity / calcium ion binding / GTP binding / ATP binding / metal ion binding / nucleus / cytoplasm 類似検索 - 分子機能
: / WDR90, 4th beta-propeller / : / : / ODAD1 central coiled coil region / Protein Flattop / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 ...: / WDR90, 4th beta-propeller / : / : / ODAD1 central coiled coil region / Protein Flattop / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 161-like domain / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / : / Parkin co-regulated protein / Parkin co-regulated protein / EF-hand domain / Nucleoside diphosphate kinase, active site / Nucleoside diphosphate kinase (NDPK) active site signature. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Heat shock protein 70kD, peptide-binding domain superfamily / Heat shock protein 70 family / Hsp70 protein / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Nucleotide-diphospho-sugar transferases / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD domain, G-beta repeat / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily 類似検索 - ドメイン・相同性
Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 52 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Trichohyalin-plectin-homology domain-containing protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein ...Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 52 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Trichohyalin-plectin-homology domain-containing protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / EF-hand domain-containing protein / Cilia- and flagella-associated protein 45 / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 20 / Protein Flattop homolog / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Outer dynein arm protein 1 / Nucleoside diphosphate kinase / Uncharacterized protein / Parkin-co-regulated gene product / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Outer dynein arm-docking complex protein DC3 / RIB72 protein / Flagellar protofilament ribbon protein 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM032843
米国
引用
ジャーナル: Cell / 年: 2019 タイトル: Structure of the Decorated Ciliary Doublet Microtubule. 著者: Meisheng Ma / Mihaela Stoyanova / Griffin Rademacher / Susan K Dutcher / Alan Brown / Rui Zhang / 要旨: The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility ...The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.
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Chlamydomonas reinhardtii (クラミドモナス)
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米国, 1件 
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emd_20631.png
http://ftp.pdbj.org/pub/emdb/structures/EMD-20631
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