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- EMDB-19968: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 b... -

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Basic information

Entry
Database: EMDB / ID: EMD-19968
TitleCryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete
Map dataPhage phi812 baseplate in the pre-contraction state - complete; postprocessed map
Sample
  • Virus: Staphylococcus phage 812 (virus)
    • Complex: Tail
      • Complex: Tail tube
        • Protein or peptide: x 1 types
      • Complex: Tail sheath
        • Protein or peptide: x 1 types
      • Complex: Baseplate
        • Complex: Baseplate protein
          • Protein or peptide: x 1 types
        • Complex: Baseplate
          • Protein or peptide: x 11 types
Keywordsbacteriophage / phage / contractile / phi812 / baseplate / VIRUS
Function / homology
Function and homology information


symbiont-mediated cytolysis of host cell / hydrolase activity, acting on glycosyl bonds / phosphoric diester hydrolase activity / cysteine-type peptidase activity / lipid metabolic process
Similarity search - Function
: / ORF68-like, C-terminal domain / : / Putative phage tail tube initiator protein / Domain of unknown function DUF4815 / Domain of unknown function (DUF4815) / Protein of unknown function DUF2634 / Contractile injection system sheath initiator / CHAP domain profile. / CHAP domain ...: / ORF68-like, C-terminal domain / : / Putative phage tail tube initiator protein / Domain of unknown function DUF4815 / Domain of unknown function (DUF4815) / Protein of unknown function DUF2634 / Contractile injection system sheath initiator / CHAP domain profile. / CHAP domain / CHAP domain / Glycerophosphodiester phosphodiesterase domain / Glycerophosphoryl diester phosphodiesterase family / GP-PDE domain profile. / Baseplate protein J-like / Baseplate J-like protein barrel domain / NlpC/P60 domain profile. / Endopeptidase, NLPC/P60 domain / Carbohydrate-binding, CenC-like / Carbohydrate binding domain / PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily / LysM domain superfamily / LysM domain / Papain-like cysteine peptidase superfamily
Similarity search - Domain/homology
Baseplate component / Baseplate wedge subunit / NlpC/P60 domain-containing protein / CBM-cenC domain-containing protein / Baseplate component / GP-PDE domain-containing protein / TmpF / Baseplate protein / Receptor binding protein / Major tail sheath protein ...Baseplate component / Baseplate wedge subunit / NlpC/P60 domain-containing protein / CBM-cenC domain-containing protein / Baseplate component / GP-PDE domain-containing protein / TmpF / Baseplate protein / Receptor binding protein / Major tail sheath protein / Peptidase C51 domain-containing protein / Putative baseplate component / DUF4815 domain-containing protein / Capsid protein
Similarity search - Component
Biological speciesStaphylococcus phage 812 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.3 Å
AuthorsBinovsky J / Siborova M / Baska R / Pichel-Beleiro A / Skubnik K / Novacek J / Benesik M / van Raaij MJ / Plevka P
Funding supportEuropean Union, Czech Republic, 2 items
OrganizationGrant numberCountry
European Research Council (ERC)101043452European Union
Other governmentLX22NP05103 Czech Republic
CitationJournal: To Be Published
Title: Cell attachment and tail contraction of S. aureus phage phi812
Authors: Binovsky J / Siborova M / Baska R / Pichel-Beleiro A / Skubnik K / Novacek J / Benesik M / van Raaij MJ / Plevka P
History
DepositionMar 27, 2024-
Header (metadata) releaseApr 9, 2025-
Map releaseApr 9, 2025-
UpdateApr 9, 2025-
Current statusApr 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19968.png

Downloads & links

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Map

FileDownload / File: emd_19968.map.gz / Format: CCP4 / Size: 2.2 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPhage phi812 baseplate in the pre-contraction state - complete; postprocessed map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 840 pix.
= 903. Å		1.08 Å/pix.
x 840 pix.
= 903. Å		1.08 Å/pix.
x 840 pix.
= 903. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.075 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.048402026 - 0.074789435
Average (Standard dev.)0.00029117643 (±0.0030989163)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions840840840
Spacing840840840
CellA=B=C: 903.00006 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_19968_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Phage phi812 baseplate in the pre-contraction state -...

Fileemd_19968_half_map_1.map
AnnotationPhage phi812 baseplate in the pre-contraction state - complete; half2 map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Phage phi812 baseplate in the pre-contraction state -...

Fileemd_19968_half_map_2.map
AnnotationPhage phi812 baseplate in the pre-contraction state - complete; half1 map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Staphylococcus phage 812

EntireName: Staphylococcus phage 812 (virus)
Components
  • Virus: Staphylococcus phage 812 (virus)
    • Complex: Tail
      • Complex: Tail tube
        • Protein or peptide: Capsid protein
      • Complex: Tail sheath
        • Protein or peptide: Major tail sheath protein
      • Complex: Baseplate
        • Complex: Baseplate protein
          • Protein or peptide: Baseplate protein
        • Complex: Baseplate
          • Protein or peptide: DUF4815 domain-containing protein
          • Protein or peptide: Receptor binding protein
          • Protein or peptide: CBM-cenC domain-containing protein
          • Protein or peptide: NlpC/P60 domain-containing protein
          • Protein or peptide: GP-PDE domain-containing protein
          • Protein or peptide: Baseplate component
          • Protein or peptide: Peptidase C51 domain-containing protein
          • Protein or peptide: Baseplate component
          • Protein or peptide: TmpF
          • Protein or peptide: Baseplate wedge subunit
          • Protein or peptide: Putative baseplate component

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Supramolecule #1: Staphylococcus phage 812

SupramoleculeName: Staphylococcus phage 812 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No
Host (natural)Organism: SA 812

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Supramolecule #2: Tail

SupramoleculeName: Tail / type: complex / ID: 2 / Parent: 1 / Macromolecule list: all
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Supramolecule #3: Tail tube

SupramoleculeName: Tail tube / type: complex / ID: 3 / Parent: 2 / Macromolecule list: #13
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Supramolecule #4: Tail sheath

SupramoleculeName: Tail sheath / type: complex / ID: 4 / Parent: 2 / Macromolecule list: #12
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Supramolecule #5: Baseplate

SupramoleculeName: Baseplate / type: complex / ID: 5 / Parent: 2 / Macromolecule list: #1-#11, #14
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Supramolecule #6: Baseplate protein

SupramoleculeName: Baseplate protein / type: complex / ID: 6 / Parent: 5 / Macromolecule list: #14
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Supramolecule #7: Baseplate

SupramoleculeName: Baseplate / type: complex / ID: 7 / Parent: 5 / Macromolecule list: #1-#11
Source (natural)Organism: Staphylococcus phage 812 (virus)

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Macromolecule #1: DUF4815 domain-containing protein

MacromoleculeName: DUF4815 domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 129.262961 KDa
SequenceString: MAINFKGSPY LDRFDPSKDR TKVLFNPDRP LQQAELNEMQ SIDQYYLKNL GDAIFKDGDK QSGLGFTLSE DNVLTVNPGY VYINGKIRY YDNDDSVKIT GVGKETIGIK LTERIVTPDE DASLLDQTSG VPSYFSKGAD RLEEKMSLTV NDPTSATIYT F MDGDLYIQ ...String:
MAINFKGSPY LDRFDPSKDR TKVLFNPDRP LQQAELNEMQ SIDQYYLKNL GDAIFKDGDK QSGLGFTLSE DNVLTVNPGY VYINGKIRY YDNDDSVKIT GVGKETIGIK LTERIVTPDE DASLLDQTSG VPSYFSKGAD RLEEKMSLTV NDPTSATIYT F MDGDLYIQ STNAEMDKIN KVLAERTYDE SGSYKVNGFE LFSEGNAEDD DHVSVVVDAG KAYVKGFKVD KPVSTRISVP KS YDLGTAE NESTIFNKSN NSISLANSPV KEIRRVTGQV LIEKERVTRG AQGDGQDFLS NNTAFEIVKV WTETSPGVTT KEY KQGEDF RLTDGQTIDW SPQGQEPSGG TSYYVSYKYN KRMEAGKDYE VTTQGEGLSK KWYINFTPSN GAKPIDQTVV LVDY TYYLA RKDSVFINKY GDIAILPGEP NIMRLVTPPL NTDPENLQLG TVTVLPDSDE AVCISFAITR LSMEDLQKVK TRVDN LEYN QAVNALDDGA MEGQNPLTLR SVFSEGFISL DKADITHPDF GIVFSFEDAE ATLAYTEAVN QPKIIPGDTT AHIWGR LIS APFTEERTIY QGQASETLNV NPYNIPNKQG VLKLTPSEDN WIDTENVTIT EQKTKKVTMK RFWRHNESYY GETEHYL YS NLQLDAGQKW KGETYAYDRE HGRTGTLLES GGQRTLEEMI EFIRIRDVSF EVKGLNPNDN NLYLLFDGVR CAITPATG Y RKGSEDGTIM TDAKGTAKGK FTIPAGIRCG NREVTLKNAN STSATTYTAQ GRKKTAQDII IRTRVTVNLV DPLAQSFQY DENRTISSLG LYFASKGDKQ SNVVIQIRGM GDQGYPNKTI YAETVMNADD IKVSNNASAE TRVYFDDPMM AEGGKEYAIV IITENSDYT MWVGTRTKPK IDKPNEVISG NPYLQGVLFS SSNASTWTPH QNSDLKFGIY TSKFNETATI EFEPIKDVSA D RIVLMSTY LTPERTGCTW EMKLILDDMA SSTTFDQLKW EPIGNYQDLD VLGLARQVKL RATFESNRYI SPLMSSSDLT FT TFLTELT GSYVGRAIDM TEAPYNTVRF SYEAFLPKGT KVVPKYSADD GKTWKTFTKS PTTTRANNEF TRYVIDEKVK SSG TNTKLQ VRLDLSTENS FLRPRVRRLM VTTRDE

UniProtKB: DUF4815 domain-containing protein

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Macromolecule #2: Receptor binding protein

MacromoleculeName: Receptor binding protein / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 50.474078 KDa
SequenceString: MALNFTTITE NNVIRDLTTQ VNNIGEELTK ERNIFDITDD LVYNFNKSQK IKLTDDKGLT KSYGNITALR DIKEPGYYYI GARTLATLL DRPDMESLDV VLHVVPLDTS SKVVQHLYTL STNNNQIKML YRFVSGNSSS EWQFIQGLPS NKNAVISGTN I LDIASPGV ...String:
MALNFTTITE NNVIRDLTTQ VNNIGEELTK ERNIFDITDD LVYNFNKSQK IKLTDDKGLT KSYGNITALR DIKEPGYYYI GARTLATLL DRPDMESLDV VLHVVPLDTS SKVVQHLYTL STNNNQIKML YRFVSGNSSS EWQFIQGLPS NKNAVISGTN I LDIASPGV YFVMGMTGGM PSGVSSGFLD LSVDANDNRL ARLTDAETGK EYTSIKKPTG TYTAWKKEFE PKDMEKYLLS SI RDDGSAS FPLLVYTSDS KTFQQAIIDH IDRTGQTTFT FYVQGGVSGS PMSNSCRGLF MSDTPNTSSL HGVYNAIGTD GRN VTGSVV GSNWTSPKTS PSHKELWTGA QSFLSTGTTK NLSDDISNYS YVEVYTTHKT TEKTKGNDNT GTICHKFYLD GSGT YVCSG TFVSGDRTDT KPPITEFYRV GVSFKGSTWT LVDSAVQNSK TQYVTRIIGI NMP

UniProtKB: Receptor binding protein

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Macromolecule #3: CBM-cenC domain-containing protein

MacromoleculeName: CBM-cenC domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 72.654742 KDa
SequenceString: MAFNYTPLTE TQKLKDMYPK VNDIGNFLKT EVNLSDVKQI SQPDFNNILA SIPDSGNYYV TNSKGAPSGE ATAGFVRLDK RNVNYYKIY YSPYSSNKMY IKTYANGTVY DWISFKLDEG SLYNEGNTLN VKELTESTTQ YATLVNPPKE NLNTGWVNYK E SKNGVSSL ...String:
MAFNYTPLTE TQKLKDMYPK VNDIGNFLKT EVNLSDVKQI SQPDFNNILA SIPDSGNYYV TNSKGAPSGE ATAGFVRLDK RNVNYYKIY YSPYSSNKMY IKTYANGTVY DWISFKLDEG SLYNEGNTLN VKELTESTTQ YATLVNPPKE NLNTGWVNYK E SKNGVSSL VEFNPVNSTS TFKMIRKLPV QEQKPNLLKD SLFVYPETSY SNIKTDNWDT PPFWGYSSNS GRSGVRFRGE NT VQIDDGS DTYPSVVSNR FKMGKELSVG DTVTVSVYAK INDPALLKDN LVYFELAGYD TVDDTSKNPY TGGRREITAS EIT TEWKKY SFTFTIPENT IGASGVKVNY VSLLLRMNCS SSKGNGAVVY YALPKLEKSS KVTPFITHEN DVRKYDEIWS NWQE VISKD ELKGHSPVDI EYNDYFKYQW WKSEVNEKSL KDLAMTVPQG YHTFYCQGSI AGTPKGRSIR GTIQVDYDKC DPYRA NKFV KLLFTDTEGI PYTLYYGGYN QGWKPLKQSE TSTLLWKGTL DFGSTEAVNL NDSLDNYDLI EVTYWTRSAG HFSTKR LDI KNTSNLLYIR DFNISNDSTG SSVDFFEGYC TFPTRTSVQP GMVKSITLDG STNTTKVASW NEKERIKVYN IMGINRG

UniProtKB: CBM-cenC domain-containing protein

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Macromolecule #4: NlpC/P60 domain-containing protein

MacromoleculeName: NlpC/P60 domain-containing protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 34.645887 KDa
SequenceString: MATDKEAKDV IDKFIDNVFN FDVLTKERIK EKDEEIKKIT TDDMYEKVVY IRPYVGVIQS LNPQHVQYES FSNNGYDIEA ELSFRKVSY LVDKGSIPTD SLSTLTVHLV ERNQELLIDY FDEIQDVLYG EYMEEEYVFD EDVPLSTILA LDLNDNLKSL S NIKYMFKG ...String:
MATDKEAKDV IDKFIDNVFN FDVLTKERIK EKDEEIKKIT TDDMYEKVVY IRPYVGVIQS LNPQHVQYES FSNNGYDIEA ELSFRKVSY LVDKGSIPTD SLSTLTVHLV ERNQELLIDY FDEIQDVLYG EYMEEEYVFD EDVPLSTILA LDLNDNLKSL S NIKYMFKG APKENPFGTD KDVYIDTYNL LYWLYLGEDE ELAYPMNINY FFTEGRFFTI FGKGHKYKVD VSKFIVGDIL FF GRSDTNI GIYVGDGEFI SMMGKFPKDE TPIGKYKLDD YWNEFNGRVM RFDEEVYI

UniProtKB: NlpC/P60 domain-containing protein

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Macromolecule #5: GP-PDE domain-containing protein

MacromoleculeName: GP-PDE domain-containing protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 96.199547 KDa
SequenceString: MVVRFQSSMG RSLKRVDSDD LNVKGLVLAT VSKINYKYQS VEVKVNNLTL GSRIGDDGSL AVPYPKSFIG RTPEGSVFGT KPLITEGSV VLIGFLNDDI NSPIILSVYG DNEQNKMINT NPLDGGKFDT ESVYKYSSSL YEILPSLNYK YDDGEGTSIR T YNGKSFFS ...String:
MVVRFQSSMG RSLKRVDSDD LNVKGLVLAT VSKINYKYQS VEVKVNNLTL GSRIGDDGSL AVPYPKSFIG RTPEGSVFGT KPLITEGSV VLIGFLNDDI NSPIILSVYG DNEQNKMINT NPLDGGKFDT ESVYKYSSSL YEILPSLNYK YDDGEGTSIR T YNGKSFFS MTSGEEEKPQ ATDFYTGTEY QDLFTSYYGN KTLIEPRIQK APNMLFKHQG VFYDDGTPDN HITTLFISER GD IRASVLN TETQKRTTQE MSSDGSYRVI KQDDDLMLDE AQVWIEYGIS EDNKFYIKND KHKFEFTDEG IYIDDKPMLE NLD ESIAEA MKNLNEIQKE LDDINYLLKG VGKDNLEELI ESTKESIEAS KKATSDVNRL TTQIAEVSGR TEGIITQFQK FRDE TFKDF YEDASTVINE VNQNFPTMKT DVKTLKTKVD NLEKTEIPNI KTRLTELENN NNNADKIISD RGEHIGAMIQ LEENV TVPM RKYMPIPWSK VTYNNAEFWD SNNPTRLVVP KGITKVRVAG NVLWDSNATG QRMLRILKNG TYSIGLPYTR DVAIST APQ NGTSGVIPVK EGDYFEFEAF QDSEGDRQFR ADPYTWFSIE AIELETETME KDFMLIGHRG ATGYTDEHTI KGYQMAL DK GADYIELDLQ LTKDNKLLCM HDSTIDRTTT GTGKVGDMTL SYIQTNFTSL NGEPIPSLDD VLNHFGTKVK YYIETKRP F DANMDRELLT QLKAKGLIGI GSERFQVIIQ SFARESLINI HNQFSNIPLA YLTSTFSESE MDDCLSYGFY AIAPKYTTI TKELVDLAHS KGLKVHAWTV NTKEEMQSLI QMGVDGFFTN YLDEYKKI

UniProtKB: GP-PDE domain-containing protein

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Macromolecule #6: Baseplate component

MacromoleculeName: Baseplate component / type: protein_or_peptide / ID: 6 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 39.248859 KDa
SequenceString: MKTRKLTNIL SKLIDKTMAG TSKITDFTPG SASRSLLEAV SLEIEQFYIL TKENIDWGIQ EGIIEAFDFQ KRQSKRAYGD VTIQFYQPL DMRMYIPAGT TFTSTRQEYP QQFETLVDYY AEPDSTEIVV EVYCKETGVA GNVPEGTINT IASGSSLIRS V NNEYSFNT ...String:
MKTRKLTNIL SKLIDKTMAG TSKITDFTPG SASRSLLEAV SLEIEQFYIL TKENIDWGIQ EGIIEAFDFQ KRQSKRAYGD VTIQFYQPL DMRMYIPAGT TFTSTRQEYP QQFETLVDYY AEPDSTEIVV EVYCKETGVA GNVPEGTINT IASGSSLIRS V NNEYSFNT GTKEESQEDF KRRFHSFVES RGRATNKSVR YGALQIPDVE GVYVYEETGH ITVFAHDRNG NLSDTLKEDI ID ALQDYRP SGIMLDVTGV EKEEVNVSAT VTISNKSRIG DTLQKHIESV IRSYLNNLKT SDDLIITDLI QAIMNIDDVL IYD VSFDNL DENIIVPPQG IIRAGEIKVE LK

UniProtKB: Baseplate component

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Macromolecule #7: Peptidase C51 domain-containing protein

MacromoleculeName: Peptidase C51 domain-containing protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 91.364289 KDa
SequenceString: MRRIRRPKVR IEIVTDDNTF TLRFEDTRDY NGDEFGAKLL GFQTKNSMED DSSVFQINMA GDTYWDKLVM ANDIIRIFIT PNDDPNDKE GKQERLIQVG MVSQVSKVGS YGNDQTQFRI TGQSFVKPFM KFGLGVIQEV QAVLPEVGWL IDGDGDNEVK F TGSSAHEV ...String:
MRRIRRPKVR IEIVTDDNTF TLRFEDTRDY NGDEFGAKLL GFQTKNSMED DSSVFQINMA GDTYWDKLVM ANDIIRIFIT PNDDPNDKE GKQERLIQVG MVSQVSKVGS YGNDQTQFRI TGQSFVKPFM KFGLGVIQEV QAVLPEVGWL IDGDGDNEVK F TGSSAHEV MTGIIRRFIP YMKYNYTEKT YNTIDNYLDY DDLSSWDEFE KLTEVSAFTN FDGSLKQLMD MVTARPFNEL FF KNSEKTP GKAQLVLRKT PFNPTEWRAL DMIKVPTEDF IEEDVGKSDV ETYSIFTATP AGMLKELNGD VFSKPQFHPE LTD RYGYTK FEVENIYLST KSGSATEDSD SSGDDNGTER GTYSKIMKDL SNYGRDNISK GIDKYTSKLS SKYKNLKKAQ AKKI IEKFV KEGKVTEKEY EKITGNKVDD ELTSDNRPKL TKDKLKSILK EKFKTQDDFN NSKKKKKAKT DALKELTTKY RFGNK THAT TLLDEYIKYK GEPPNDEAFD KYLKAIEGVS NVATDTGSDA SDSPLVMFSR MLFNWYHGNP NFYAGDIIVL GDPKYD LGK RLFIEDKQRG DTWEFYIESV EHKFDYKQGY YTTVGVTRGL KDAILEDGKG SPHRFAGLWN QSSDFMGGLM GEDTSKE LK EKGVAEKQSS GDKDGGSDSG GAQDGGSLDS LKKYNGKLPK HDPSFVQPGN RHYKYQCTWY AYNRRGQLGI PVPLWGDA A DWIGGAKGAG YGVGRTPKQG ACVIWQRGVQ GGSPQYGHVA FVEKVLDGGK KIFISEHNYA TPNGYGTRTI DMSSAIGKN AQFIYDKK

UniProtKB: Peptidase C51 domain-containing protein

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Macromolecule #8: Baseplate component

MacromoleculeName: Baseplate component / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 19.998748 KDa
SequenceString:
MNNFIPQPQG LLRFLNTLDT DLTSSHMNLL DEEVSFVSKF YTPQLQLSEL AKKVLTNIKT DDIPVLEREF NDNTIIHKAN DTLLKVQAP RMYMILQSIV LEAYAIVNCF VENPSSLKYL TEEDVSITRE NLNYVADYLG NYDDYNSVVL DLRDLDLCFS A IELQLPLI KKEANV

UniProtKB: Baseplate component

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Macromolecule #9: TmpF

MacromoleculeName: TmpF / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 116.389945 KDa
SequenceString: MANFLKNLHP LLRRDRNKKD NQDPNFALID ALNEEMNQVE KDAIESKLQS SLKTSTSEYL DKFGDWFGVY RKTDEKDDVY RARIIKYLL LKRGTNNAII DAIKDYLGRD DIDVSVYEPF TNIFYTNKSH LNGEDHLMGY YYRFAVINVS IGDYFPVEII D VINEFKPA ...String:
MANFLKNLHP LLRRDRNKKD NQDPNFALID ALNEEMNQVE KDAIESKLQS SLKTSTSEYL DKFGDWFGVY RKTDEKDDVY RARIIKYLL LKRGTNNAII DAIKDYLGRD DIDVSVYEPF TNIFYTNKSH LNGEDHLMGY YYRFAVINVS IGDYFPVEII D VINEFKPA GVTLYVTYDG ASTIRGGAII KWLDGLPKIE TYQEFDRFTG YDDTFYGHIN MNQSKDTDNS SSDIFKTNHS LI NSLDVLT GSSSVGRQYI NYGYVTSYVY NPGMTSSVNQ ISASTEGRGQ EVPTDYYMYT STKNNNTVEL SMQTTSGVSY LYN NFNFRD YMSKYRPQVD LQSDEARRIV SDYIKELSID YYLSAVIPPD ESIEIKLQVY DFSINRWLTV SINNLSFYEK NIGS NIGYI KDYLNSELNM FTRLEINAGK RDSVDIKVNY LDLMFYYYER GIYTIKPYKA LIENYLDISR ETYVEAFKIA SLSNG DIIT KTGFQPIGYL KLVGNYENTI PSTINIVAKD TDNNPIESNE LDVYNTVENR NLLQSYKGVN TIAREITSTK EFTVSG WAK EIYSTNYLSK VLKPGKVYTL SFDMEITGND PTLKSYSDNH GIYLYSNTKG IVVNGVKSME RTIGNKVSVT QTFTAPT IT DHRLLIYTGR YTSDGKASTP PVFFNTVKIT ELKLTEGSSK LEYSPAPEDK PNVIEKGIKF NNILTNIQTL SINSDTIL K NVTLYYSYYG DSWVELKTLG NISTGETTET NNLIDLYGLQ TVDYSNINPM SKVSLRSIWN VKLGELNNQE GSLSNMPND YFNAVWQDID KLSDIELGSM RMVKDTEGGV FDGATGEIIK ATLFNVGAYT DLDMLAYTLT NYTEPLTLGS SRLISELKEE LLTSESFNV DNRIKVIDSI YEELPNTSII KNGFVEREVT GSKYLDYGLY EPIEDGTRYK LIVEGEFKDN IEFISLYNSN P NFNETFIY PSEIINGVAE KEFIAKPSTE DKPRLNTDVR IYIRPYDSTI SKVRRVELRK V

UniProtKB: TmpF

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Macromolecule #10: Baseplate wedge subunit

MacromoleculeName: Baseplate wedge subunit / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 26.611752 KDa
SequenceString: MRFKKHVVQH EETMQAIAQR YYGDVSYWID LVEHNNLKYP YLVETDEEKM KDPERLASTG DTLIIPIESD LTDVSAKEIN SRDKDVLVE LALGRDLNIT ADEKYFNEHG TSDNILAFST NGNGDLDTVK GIDNMKQQLQ ARLLTPRGSL MLHPNYGSDL H NLFGLNIP ...String:
MRFKKHVVQH EETMQAIAQR YYGDVSYWID LVEHNNLKYP YLVETDEEKM KDPERLASTG DTLIIPIESD LTDVSAKEIN SRDKDVLVE LALGRDLNIT ADEKYFNEHG TSDNILAFST NGNGDLDTVK GIDNMKQQLQ ARLLTPRGSL MLHPNYGSDL H NLFGLNIP EQATLIEMEV LRTLTSDNRV KSANLIDWKI QGNVYSGQFS VEIKSVEESI NFVLGQDEEG IFALFE

UniProtKB: Baseplate wedge subunit

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Macromolecule #11: Putative baseplate component

MacromoleculeName: Putative baseplate component / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 29.381604 KDa
SequenceString: MPQSDGISNL HRIALRFPKE GGGYDMYRFK VNPENYTIDS PQRTTAIKTK SDIVIEDYGK DIEVINFTGT TGFRPVREAD GLKTGKQKM EELQSRVSEY AMQGGSGNVS GSYLQFFNFT DDSYYKVHLA PQGLKITRSK DEPLLFRYEI TLVVIGSLTE A DRSAVTTE ...String:
MPQSDGISNL HRIALRFPKE GGGYDMYRFK VNPENYTIDS PQRTTAIKTK SDIVIEDYGK DIEVINFTGT TGFRPVREAD GLKTGKQKM EELQSRVSEY AMQGGSGNVS GSYLQFFNFT DDSYYKVHLA PQGLKITRSK DEPLLFRYEI TLVVIGSLTE A DRSAVTTE EFGNVKPNAS QRVDEGIKEL DKNARKTRDR NNQEISRREN TIPKSTGDNT NEGNRLKQSF PSSSIYNPRQ ST NGLKGNI DNMALIIGYG DGGVSS

UniProtKB: Putative baseplate component

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Macromolecule #12: Major tail sheath protein

MacromoleculeName: Major tail sheath protein / type: protein_or_peptide / ID: 12 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 64.559008 KDa
SequenceString: MAVEPFPRRP ITRPHASIEV DTSGIGGSAG SSEKVFCLIG QAEGGEPNTV YELRNYSQAK RLFRSGELLD AIELAWGSNP NYTAGRILA MRIEDAKPAS AEIGGLKITS KIYGNVANNI QVGLEKNTLS DSLRLRVIFQ DDRFNEVYDN IGNIFTIKYK G EEANATFS ...String:
MAVEPFPRRP ITRPHASIEV DTSGIGGSAG SSEKVFCLIG QAEGGEPNTV YELRNYSQAK RLFRSGELLD AIELAWGSNP NYTAGRILA MRIEDAKPAS AEIGGLKITS KIYGNVANNI QVGLEKNTLS DSLRLRVIFQ DDRFNEVYDN IGNIFTIKYK G EEANATFS VEHDEETQKA SRLVLKVGDQ EVKSYDLTGG AYDYTNAIIT DINQLPDFEA KLSPFGDKNL ESSKLDKIEN AN IKDKAVY VKAVFGDLEK QTAYNGIVSF EQLNAEGEVP SNVEVEAGEE SATVTATSPI KTIEPFELTK LKGGTNGEPP ATW ADKLDK FAHEGGYYIV PLSSKQSVHA EVASFVKERS DAGEPMRAIV GGGFNESKEQ LFGRQASLSN PRVSLVANSG TFVM DDGRK NHVPAYMVAV ALGGLASGLE IGESITFKPL RVSSLDQIYE SIDLDELNEN GIISIEFVRN RTNTFFRIVD DVTTF NDKS DPVKAEMAVG EANDFLVSEL KVQLEDQFIG TRTINTSASI IKDFIQSYLG RKKRDNEIQD FPAEDVQVIV EGNEAR ISM TVYPIRSFKK ISVSLVYKQQ TLQA

UniProtKB: Major tail sheath protein

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Macromolecule #13: Capsid protein

MacromoleculeName: Capsid protein / type: protein_or_peptide / ID: 13 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 15.94297 KDa
SequenceString:
MASEAKQTVH TGNTVLLMIK GKPVGRAQSA SGQREYGTTG VYEIGSIMPQ EHVYLRYEGT ITVERLRMKK ENFADLGYAS LGEEILKKD IIDILVVDNL TKQVIISYHG CSANNYNETW QTNEIVTEEI EFSYLTASDK ART

UniProtKB: Capsid protein

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Macromolecule #14: Baseplate protein

MacromoleculeName: Baseplate protein / type: protein_or_peptide / ID: 14
Details: recombinantly expressed structure; protein-linker-His6tag; linker+His6tag: DPNSSSVDKLAAALEHHHHHH
Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Staphylococcus phage 812 (virus)
Molecular weightTheoretical: 21.588104 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MAIATYNSHV ELAKYLVSKA DSVYLTIGKS TPWSNETNPP QPDENATVLQ EVIGYKKATK VTLVRPSKSP EDDNKNLISY GNKSWVEVT PENAKAEGAK WVYLESSIVG DELPLGTYRQ VGFVMDLVAK SGISKFNLVP SEVESTGTLL FFDNKQFQNR S EQTTAKER ...String:
MAIATYNSHV ELAKYLVSKA DSVYLTIGKS TPWSNETNPP QPDENATVLQ EVIGYKKATK VTLVRPSKSP EDDNKNLISY GNKSWVEVT PENAKAEGAK WVYLESSIVG DELPLGTYRQ VGFVMDLVAK SGISKFNLVP SEVESTGTLL FFDNKQFQNR S EQTTAKER FIVEVDPNSS SVDKLAAALE HHHHHH

UniProtKB: Baseplate protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8 / Details: 50mM Tris, 10mM NaCl, 10mM CaCl2
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Average exposure time: 1.0 sec. / Average electron dose: 48.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 75000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 23421
Startup modelType of model: EMDB MAP
EMDB ID:

8210

Final reconstructionApplied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 7.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 9705
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION / Details: C3 symmetry imposed
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-9euf:
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete

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