EMDB-18332: B. subtilis ApdA-stalled ribosomal complex

Basic information

Entry

Database: EMDB / ID: EMD-18332

• Title: B. subtilis ApdA-stalled ribosomal complex
• Map data: Postprocessed final map

Sample

• Complex: B. subtilis ApdA-stalled ribosomal complex
  • Protein or peptide: x 39 types
  • RNA: x 4 types
• Protein or peptide: x 5 types
• RNA: x 1 types
• Ligand: x 5 types

• Keywords: Stalling / Nascent chain / elongation arrest / regulation / RIBOSOME

Function / homology

Function:

positive regulation of rRNA processing / nucleoid / rRNA processing / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosomal small subunit assembly / large ribosomal subunit rRNA binding / 5S rRNA binding / transferase activity / small ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / response to antibiotic / mRNA binding / DNA binding / RNA binding / zinc ion binding / metal ion binding / cytosol / cytoplasm

Domain/homology:

: / Ribosomal protein S14, type Z / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein L20 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / : / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L21 / Ribosomal protein S15, bacterial-type / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein S6 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein S6 / Ribosomal L32p protein family / Ribosomal protein S6 superfamily / Ribosomal protein L32p / Ribosomal protein S12, bacterial-type / Ribosomal protein L24 / Ribosomal protein L34 / Ribosomal protein L34

Component:

Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein bL31

• Biological species: Amycolatopsis japonica (bacteria) / Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 2.3 Å
• Authors: Morici M / Wilson DN

Funding support

Germany, 1 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany

• Citation: Journal: Nat Commun / Year: 2024; Title: RAPP-containing arrest peptides induce translational stalling by short circuiting the ribosomal peptidyltransferase activity.; Authors: Martino Morici / Sara Gabrielli / Keigo Fujiwara / Helge Paternoga / Bertrand Beckert / Lars V Bock / Shinobu Chiba / Daniel N Wilson / ; Abstract: Arrest peptides containing RAPP (ArgAlaProPro) motifs have been discovered in both Gram-positive and Gram-negative bacteria, where they are thought to regulate expression of important protein localization machinery components. Here we determine cryo-EM structures of ribosomes stalled on RAPP arrest motifs in both Bacillus subtilis and Escherichia coli. Together with molecular dynamics simulations, our structures reveal that the RAPP motifs allow full accommodation of the A-site tRNA, but prevent the subsequent peptide bond from forming. Our data support a model where the RAP in the P-site interacts and stabilizes a single hydrogen atom on the Pro-tRNA in the A-site, thereby preventing an optimal geometry for the nucleophilic attack required for peptide bond formation to occur. This mechanism to short circuit the ribosomal peptidyltransferase activity is likely to operate for the majority of other RAPP-like arrest peptides found across diverse bacterial phylogenies.

History

Deposition	Aug 28, 2023	-
Header (metadata) release	Mar 20, 2024	-
Map release	Mar 20, 2024	-
Update	Nov 13, 2024	-
Current status	Nov 13, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_18332.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Postprocessed final map
• Voxel size: X=Y=Z: 0.82 Å

Density

Contour Level	By AUTHOR: 0.052
Minimum - Maximum	-0.13928296 - 0.34229735
Average (Standard dev.)	0.0008228579 (±0.00951122)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 344.4 Å α=β=γ: 90.0 °

Supplemental data

Additional map: Preprocessed map

• File: emd_18332_additional_1.map
• Annotation: Preprocessed map

Additional map: Filtered 3D-refined map based on local resolution as...

• File: emd_18332_additional_2.map
• Annotation: Filtered 3D-refined map based on local resolution as calculated by Bsoft

Half map: Half-map 1

• File: emd_18332_half_map_1.map
• Annotation: Half-map 1

Half map: Half-map 2

• File: emd_18332_half_map_2.map
• Annotation: Half-map 2

Sample components

Entire : B. subtilis ApdA-stalled ribosomal complex

• Entire: Name: B. subtilis ApdA-stalled ribosomal complex

Components

• Complex: B. subtilis ApdA-stalled ribosomal complex
  • Protein or peptide: 50S ribosomal protein L34
  • Protein or peptide: 50S ribosomal protein L35
  • RNA: 5S rRNA
  • Protein or peptide: 50S ribosomal protein L2
  • Protein or peptide: 50S ribosomal protein L3
  • Protein or peptide: 50S ribosomal protein L4
  • Protein or peptide: 50S ribosomal protein L5
  • Protein or peptide: Large ribosomal subunit protein uL6
  • Protein or peptide: 50S ribosomal protein L13
  • Protein or peptide: 50S ribosomal protein L14
  • Protein or peptide: 50S ribosomal protein L15
  • Protein or peptide: 50S ribosomal protein L16
  • Protein or peptide: 50S ribosomal protein L17
  • Protein or peptide: 50S ribosomal protein L18
  • Protein or peptide: 50S ribosomal protein L19
  • Protein or peptide: Large ribosomal subunit protein bL20
  • Protein or peptide: 50S ribosomal protein L21
  • Protein or peptide: 50S ribosomal protein L22
  • Protein or peptide: Large ribosomal subunit protein uL23
  • Protein or peptide: 50S ribosomal protein L24
  • Protein or peptide: Large ribosomal subunit protein bL27
  • Protein or peptide: 50S ribosomal protein L28
  • Protein or peptide: 50S ribosomal protein L29
  • Protein or peptide: Large ribosomal subunit protein uL30
  • RNA: 16S rRNA
  • Protein or peptide: 30S ribosomal protein S5
  • Protein or peptide: 30S ribosomal protein S11
  • Protein or peptide: 30S ribosomal protein S12
  • Protein or peptide: 30S ribosomal protein S15
  • Protein or peptide: 30S ribosomal protein S17
  • Protein or peptide: 30S ribosomal protein S20
  • RNA: Pro-tRNA
  • RNA: mRNA
  • Protein or peptide: Small ribosomal subunit protein uS3
  • Protein or peptide: Small ribosomal subunit protein uS7
  • Protein or peptide: 30S ribosomal protein S9
  • Protein or peptide: 30S ribosomal protein S10
  • Protein or peptide: 30S ribosomal protein S13
  • Protein or peptide: 30S ribosomal protein S19
  • Protein or peptide: 30S ribosomal protein S8
  • Protein or peptide: 30S ribosomal protein S18
  • Protein or peptide: 30S ribosomal protein S6
  • Protein or peptide: ApdA nascent chain
• Protein or peptide: 50S ribosomal protein L32
• Protein or peptide: 50S ribosomal protein L33 1
• Protein or peptide: 50S ribosomal protein L36
• Protein or peptide: Large ribosomal subunit protein bL31-A
• Protein or peptide: 30S ribosomal protein S14
• RNA: 23S rRNA
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM ION
• Ligand: PROLINE
• Ligand: water

Supramolecule #1: B. subtilis ApdA-stalled ribosomal complex

• Supramolecule: Name: B. subtilis ApdA-stalled ribosomal complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #3-#4, #8-#43, #45-#49
• Source (natural): Organism: Amycolatopsis japonica (bacteria)

Macromolecule #1: 50S ribosomal protein L32

• Macromolecule: Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.745073 KDa

Sequence

String:

MAVPFRRTSK MKKRLRRTHF KLNVPGMTEC PSCGEMKLSH RVCKACGSYN GKDINVKSN

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #2: 50S ribosomal protein L33 1

• Macromolecule: Name: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 5.915875 KDa

Sequence

String:

MRVNITLACT ECGERNYISK KNKRNNPDRV EFKKYCPRDK KSTLHRETK

UniProtKB: Large ribosomal subunit protein bL33A

Macromolecule #3: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 5.271332 KDa

Sequence

String:

MKRTFQPNNR KRSKVHGFRS RMSSKNGRLV LARRRRKGRK VLSA

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #4: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.581128 KDa

Sequence

String:

MPKMKTHRGS AKRFKKTGSG KLKRSHAYTS HLFANKSQKQ KRKLRKSAVV SAGDFKRIKQ MLANIK

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #5: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 4.318422 KDa

Sequence

String:

MKVRPSVKPI CEKCKVIRRK GKVMVICENP KHKQKQG

UniProtKB: Large ribosomal subunit protein bL36

Macromolecule #6: Large ribosomal subunit protein bL31-A

• Macromolecule: Name: Large ribosomal subunit protein bL31-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.330452 KDa

Sequence

String:

MKAGIHPNFK KATVKCACGN EFETGSVKEE VRVEICSECH PFYTGRKFAS ADGRVDRFNK KYGLK

UniProtKB: Large ribosomal subunit protein bL31

Macromolecule #9: 50S ribosomal protein L2

• Macromolecule: Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 30.335125 KDa

Sequence

String:

MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT VRHQGGGHKR QYRVIDFKRD KDGIPGRVAT VEYDPNRSA NIALINYADG EKRYILAPKG IQVGTEIMSG PEADIKVGNA LPLINIPVGT VVHNIELKPG KGGQLVRSAG T SAQVLGKE GKYVLVRLNS GEVRMILSAC RASIGQVGNE QHELINIGKA GRSRWKGIRP TVRGSVMNPN DHPHGGGEGR AP IGRKSPM SPWGKPTLGF KTRKKKNKSD KFIVRRRKNK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #10: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 22.723348 KDa

Sequence

String:

MTKGILGRKI GMTQVFAENG DLIPVTVIEA APNVVLQKKT AENDGYEAIQ LGFDDKREKL SNKPEKGHVA KAETAPKRFV KELRGVEMD AYEVGQEVKV EIFSAGEIVD VTGVSKGKGF QGAIKRHGQS RGPMSHGSRY HRRPGSMGPV DPNRVFKGKL L PGRMGGEQ ITVQNLEIVK VDAERNLLLI KGNVPGAKKS LITVKSAVKS K

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #11: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 22.424951 KDa

Sequence

String:

MPKVALYNQN GSTAGDIELN ASVFGIEPNE SVVFDAILMQ RASLRQGTHK VKNRSEVRGG GRKPWRQKGT GRARQGSIRS PQWRGGGVV FGPTPRSYSY KLPKKVRRLA IKSVLSSKVI DNNIIVLEDL TLDTAKTKEM AAILKGLSVE KKALIVTADA N EAVALSAR NIPGVTVVEA NGINVLDVVN HEKLLITKAA VEKVEEVLA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #12: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 20.177564 KDa

Sequence

String:

MNRLKEKYNK EIAPALMTKF NYDSVMQVPK IEKIVINMGV GDAVQNAKAI DSAVEELTFI AGQKPVVTRA KKSIAGFRLR EGMPIGAKV TLRGERMYDF LDKLISVSLP RVRDFRGVSK KSFDGRGNYT LGIKEQLIFP EIDYDKVTKV RGMDIVIVTT A NTDEEARE LLTQVGMPFQ K

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #13: Large ribosomal subunit protein uL6

• Macromolecule: Name: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 19.543389 KDa

Sequence

String:

MSRVGKKLLE IPSDVTVTLN DNNTVAVKGP KGELTRTFHP DMEIKVEDNV LTVARPSDQK EHRALHGTTR SLLGNMVEGV SKGFERGLE LVGVGYRASK SGNKLVLNVG YSHPVEIVPE EGIEIEVPSQ TKVVVKGTDK ERVGAIAANI RAVRSPEPYK G KGIRYEGE VVRRKEGKSA K

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #14: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 16.407104 KDa

Sequence

String:

MRTTPMANAS TIERKWLVVD AAGKTLGRLS SEVAAILRGK HKPTYTPHVD TGDHVIIINA EKIELTGKKL TDKIYYRHTQ HPGGLKSRT ALEMRTNYPE KMLELAIKGM LPKGSLGRQM FKKLNVYRGS EHPHEAQKPE VYELRG

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #15: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.175288 KDa

Sequence

String:

MIQQETRLKV ADNSGAREVL TIKVLGGSGR KTANIGDVIV CTVKQATPGG VVKKGEVVKA VIVRTKSGAR RSDGSYISFD ENACVIIRD DKSPRGTRIF GPVARELREN NFMKIVSLAP EVI

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #16: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 15.410694 KDa

Sequence

String:

MKLHELKPSE GSRKTRNRVG RGIGSGNGKT AGKGHKGQNA RSGGGVRPGF EGGQMPLFQR LPKRGFTNIN RKEYAVVNLD KLNGFAEGT EVTPELLLET GVISKLNAGV KILGNGKLEK KLTVKANKFS ASAKEAVEAA GGTAEVI

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #17: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 16.223049 KDa

Sequence

String:

MLLPKRVKYR REHRGKMRGR AKGGTEVHFG EFGIQALEAS WITNRQIEAA RIAMTRYMKR GGKVWIKIFP SKPYTAKPLE VRMGSGKGA PEGWVAVVKP GKVLFEISGV SEEVAREALR LASHKLPIKT KFVKREEIGG ESNES

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #18: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.774806 KDa

Sequence

String:

MSYRKLGRTS AQRKAMLRDL TTDLIINERI ETTETRAKEL RSVVEKMITL GKRGDLHARR QAAAYIRNEV ANEENNQDAL QKLFSDIAT RYEERQGGYT RIMKLGPRRG DGAPMAIIEL V

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #19: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 12.993829 KDa

Sequence

String:

MITKTSKNAA RLKRHARVRA KLSGTAERPR LNVFRSNKHI YAQIIDDVNG VTLASASTLD KDLNVESTGD TSAATKVGEL VAKRAAEKG ISDVVFDRGG YLYHGRVKAL ADAAREAGLK F

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #20: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.416853 KDa

Sequence

String:

MQKLIEDITK EQLRTDLPAF RPGDTLRVHV KVVEGNRERI QIFEGVVIKR RGGGISETFT VRKISYGVGV ERTFPVHTPK IAKIEVVRY GKVRRAKLYY LRELRGKAAR IKEIRR

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #21: Large ribosomal subunit protein bL20

• Macromolecule: Name: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.669189 KDa

Sequence

String:

MPRVKGGTVT RKRRKKVLKL AKGYFGSKHT LYKVANQQVM KSGNYAFRDR RQKKRDFRKL WITRINAAAR MNGLSYSRLM HGLKLSGIE VNRKMLADLA VNDLTAFNQL ADAAKAQLNK

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #22: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.296081 KDa

Sequence

String:

MYAIIKTGGK QIKVEEGQTV YIEKLAAEAG ETVTFEDVLF VGGDNVKVGN PTVEGATVTA KVEKQGRAKK ITVFRYKPKK NVHKKQGHR QPYTKVTIEK INA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #23: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 12.481608 KDa

Sequence

String:

MQAKAVARTV RIAPRKARLV MDLIRGKQVG EAVSILNLTP RAASPIIEKV LKSAIANAEH NYEMDANNLV ISQAFVDEGP TLKRFRPRA MGRASQINKR TSHITIVVSE KKEG

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #24: Large ribosomal subunit protein uL23

• Macromolecule: Name: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.978813 KDa

Sequence

String:

MKDPRDVLKR PVITERSADL MTEKKYTFEV DVRANKTEVK DAVESIFGVK VDKVNIMNYK GKSKRVGRYT GMTSRRRKAI VKLTADSKE IEIFEA

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #25: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.16612 KDa

Sequence

String:

MHVKKGDKVM VISGKDKGKQ GTILAAFPKK DRVLVEGVNM VKKHSKPTQA NPQGGISNQE APIHVSNVMP LDPKTGEVTR VGYKVEDGK KVRVAKKSGQ VLDK

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #26: Large ribosomal subunit protein bL27

• Macromolecule: Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.391855 KDa

Sequence

String:

MLRLDLQFFA SKKGVGSTKN GRDSEAKRLG AKRADGQFVT GGSILYRQRG TKIYPGENVG RGGDDTLFAK IDGTVKFERF GRDRKKVSV YPVAQ

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #27: 50S ribosomal protein L28

• Macromolecule: Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.826144 KDa

Sequence

String:

MARKCVITGK KTTAGNNRSH AMNASKRTWG ANLQKVRILV NGKPKKVYVS ARALKSGKVE RV

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #28: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.728029 KDa

Sequence

String:

MKANEIRDLT TAEIEQKVKS LKEELFNLRF QLATGQLENT ARIREVRKAI ARMKTVIRER EIAANK

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #29: Large ribosomal subunit protein uL30

• Macromolecule: Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 6.650795 KDa

Sequence

String:

MAKLEITLKR SVIGRPEDQR VTVRTLGLKK TNQTVVHEDN AAIRGMINKV SHLVSVKEQ

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #31: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 17.650625 KDa

Sequence

String:

MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI RKAVEDAKKN LIEVPMVGTT IPHEIIGRF GAGNILLKPA SEGTGVIAGG PVRAVLELAG VADILSKSLG SNTPINMIRA TLQGLSELKR AEDVAKLRGK S VEELLG

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #32: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.952 KDa

Sequence

String:

MAAARKSNTR KRRVKKNIES GIAHIRSTFN NTIVTITDTH GNAISWSSAG ALGFRGSRKS TPFAAQMAAE TAAKGSIEHG LKTLEVTVK GPGSGREAAI RALQAAGLEV TAIRDVTPVP HNGCRPPKRR RV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #33: 30S ribosomal protein S12

• Macromolecule: Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 15.248736 KDa

Sequence

String:

MPTINQLIRK GRVSKVENSK SPALNKGYNS FKKEHTNVSS PQKRGVCTRV GTMTPKKPNS ALRKYARVRL TNGIEVTAYI PGIGHNLQE HSVVLIRGGR VKDLPGVRYH IVRGALDTAG VENRAQGRSK YGTKKPKAK

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #34: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.597224 KDa

Sequence

String:

MAITQERKNQ LINEFKTHES DTGSPEVQIA ILTDSINNLN EHLRTHKKDH HSRRGLLKMV GKRRNLLTYL RNKDVTRYRE LINKLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #35: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.220979 KDa

Sequence

String:

MSERNQRKVY QGRVVSDKMD KTITVVVETY KKHTLYGKRV KYSKKFKAHD ENNQAKIGDI VKIMETRPLS ATKRFRLVEV VEEAVII

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #36: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 9.622217 KDa

Sequence

String:

MPNIKSAIKR TKTNNERRVH NATIKSAMRT AIKQVEASVA NNEADKAKTA LTEAAKRIDK AVKTGLVHKN TAARYKSRLA KKVNGLSA

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #39: Small ribosomal subunit protein uS3

• Macromolecule: Name: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 23.050357 KDa

Sequence

String:

MGQKVNPVGL RIGVIRDWES KWYAGKDYAD FLHEDLKIRE YISKRLSDAS VSKVEIERAA NRVNITIHTA KPGMVIGKGG SEVEALRKA LNSLTGKRVH INILEIKRAD LDAQLVADNI ARQLENRVSF RRAQKQQIQR TMRAGAQGVK TMVSGRLGGA D IARSEYYS EGTVPLHTLR ADIDYATSEA DTTYGKLGVK VWIYRGEV

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #40: Small ribosomal subunit protein uS7

• Macromolecule: Name: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 17.915879 KDa

Sequence

String:

MPRKGPVAKR DVLPDPIYNS KLVSRLINKM MIDGKKGKSQ TILYKSFDII KERTGNDAME VFEQALKNIM PVLEVKARRV GGANYQVPV EVRPERRTTL GLRWLVNYAR LRGEKTMEER LANEILDAAN NTGAAVKKRE DTHKMAEANK AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #41: 30S ribosomal protein S9

• Macromolecule: Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 14.335504 KDa

Sequence

String:

MAQVQYYGTG RRKSSVARVR LVPGEGRIVV NNREISEHIP SAALIEDIKQ PLTLTETAGT YDVLVNVHGG GLSGQAGAIR HGIARALLE ADPEYRTTLK RAGLLTRDAR MKERKKYGLK GARRAPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #42: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.687661 KDa

Sequence

String:

MAKQKIRIRL KAYDHRILDQ SAEKIVETAK RSGASVSGPI PLPTEKSVYT ILRAVHKYKD SREQFEMRTH KRLIDIVNPT PQTVDALMR LDLPSGVDIE IKL

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #43: 30S ribosomal protein S13

• Macromolecule: Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 13.818085 KDa

Sequence

String:

MARIAGVDIP RDKRVVISLT YIFGIGRTTA QQVLKEAGVS EDTRVRDLTE EELGKIRDII DKLKVEGDLR REVSLNIKRL IEIGSYRGI RHRRGLPVRG QNSKNNARTR KGPRRTVANK KK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #44: 30S ribosomal protein S14

• Macromolecule: Name: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 7.263803 KDa

Sequence

String:

MAKKSMIAKQ QRTPKFKVQE YTRCERCGRP HSVIRKFKLC RICFRELAYK GQIPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14B

Macromolecule #45: 30S ribosomal protein S19

• Macromolecule: Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 10.607309 KDa

Sequence

String:

MARSLKKGPF VDGHLMTKIE KLNETDKKQV VKTWSRRSTI FPQFIGHTIA VYDGRKHVPV FISEDMVGHK LGEFAPTRTY KGHASDDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #46: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 14.901427 KDa

Sequence

String:

MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII RVFLKYGQNN ERVITGLKRI SKPGLRVYA KSNEVPRVLN GLGIAIISTS QGVLTDKEAR AKQAGGEVLA YVW

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #47: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 8.990613 KDa

Sequence

String:

MAGGRRGGRA KRRKVCYFTS NGITHIDYKD VDLLKKFVSE RGKILPRRVT GTNAKYQRKL TAAIKRARQM ALLPYVSGE

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #48: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 11.140548 KDa

Sequence

String:

MRKYEVMYII RPNIDEESKK AVIERFNNVL TSNGAEITGT KDWGKRRLAY EINDFRDGFY QIVNVQSDAA AVQEFDRLAK ISDDIIRHI VVKEEE

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #49: ApdA nascent chain

• Macromolecule: Name: ApdA nascent chain / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Amycolatopsis japonica (bacteria)
• Molecular weight: Theoretical: 970.128 Da
• Recombinant expression: Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)

Sequence

String:

RLALGDRAP

Macromolecule #7: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 948.73375 KDa

Sequence

String:

GGUUAAGUUA GAAAGGGCGC ACGGUGGAUG CCUUGGCACU AGGAGCCGAU GAAGGACGGG ACGAACACCG AUAUGCUUCG GGGAGCUGU AAGCAAGCUU UGAUCCGGAG AUUUCCGAAU GGGGAAACCC ACCACUCGUA AUGGAGUGGU AUCCAUAUCU G AAUUCAUA GGAUAUGAGA AGGCAGACCC GGGGAACUGA AACAUCUAAG UACCCGGAGG AAGAGAAAGC AAAUGCGAUU CC CUGAGUA GCGGCGAGCG AAACGGGAUC AGCCCAAACC AAGAGGCUUG CCUCUUGGGG UUGUAGGACA CUCUGUACGG AGU UACAAA GGAACGAGGU AGAUGAAGAG GUCUGGAAAG GCCCGCCAUA GGAGGUAACA GCCCUGUAGU CAAAACUUCG UUCU CUCCU GAGUGGAUCC UGAGUACGGC GGAACACGUG AAAUUCCGUC GGAAUCCGGG AGGACCAUCU CCCAAGGCUA AAUAC UCCC UAGUGACCGA UAGUGAACCA GUACCGUGAG GGAAAGGUGA AAAGCACCCC GGAAGGGGAG UGAAAGAGAU CCUGAA ACC GUGUGCCUAC AAGUAGUCAG AGCCCGUUAA CGGGUGAUGG CGUGCCUUUU GUAGAAUGAA CCGGCGAGUU ACGAUCU CG UGCAAGGUUA AGCAGAAGAU GCGGAGCCGC AGCGAAAGCG AGUCUGAAUA GGGCGCAUGA GUACGUGGUC GUAGACCC G AAACCAGGUG AUCUACCCAU GUCCAGGGUG AAGUUCAGGU AACACUGAAU GGAGGCCCGA ACCCACGCAC GUUGAAAAG UGCGGGGAUG AGGUGUGGGU AGGGGUGAAA UGCCAAUCGA ACCUGGAGAU AGCUGGUUCU CUCCGAAAUA GCUUUAGGGC UAGCCUCAA GGUAAGAGUC UUGGAGGUAG AGCACUGAUU GGACUAGGGG CCCCUACCGG GUUACCGAAU UCAGUCAAAC U CCGAAUGC CAAUGACUUA UCCUUGGGAG UCAGACUGCG AGUGAUAAGA UCCGUAGUCG AAAGGGAAAC AGCCCAGACC GC CAGCUAA GGUCCCAAAG UAUACGUUAA GUGGAAAAGG AUGUGGAGUU GCUUAGACAA CCAGGAUGUU GGCUUAGAAG CAG CCACCA UUUAAAGAGU GCGUAAUAGC UCACUGGUCG AGUGACUCUG CGCCGAAAAU GUACCGGGGC UAAACGUAUC ACCG AAGCU GCGGACUGUU CUUCGAACAG UGGUAGGAGA GCGUUCUAAG GGCUGUGAAG CCAGACCGGA AGGACUGGUG GAGCG CUUA GAAGUGAGAA UGCCGGUAUG AGUAGCGAAA GAGGGGUGAG AAUCCCCUCC ACCGAAUGCC UAAGGUUUCC UGAGGA AGG CUCGUCCGCU CAGGGUUAGU CGGGACCUAA GCCGAGGCCG AAAGGCGUAG GCGAUGGACA ACAGGUUGAU AUUCCUG UA CCACCUCCUC ACCAUUUGAG CAAUGGGGGG ACGCAGGAGG AUAGGGUAAG CGCGGUAUUG GAUAUCCGCG UCCAAGCA G UUAGGCUGGG AAAUAGGCAA AUCCGUUUCC CAUAAGGCUG AGCUGUGAUG GCGAGCGAAA UAUAGUAGCG AAGUUCCUG AUUCCACACU GCCAAGAAAA GCCUCUAGCG AGGUGAGAGG UGCCCGUACC GCAAACCGAC ACAGGUAGGC GAGGAGAGAA UCCUAAGGU GAUCGAGAGA ACUCUCGUUA AGGAACUCGG CAAAAUGACC CCGUAACUUC GGGAGAAGGG GUGCUCUGUU A GGGUGCAA GCCCGAGAGA GCCGCAGUGA AUAGGCCCAG GCGACUGUUU AGCAAAAACA CAGGUCUCUG CGAAGCCGUA AG GCGAAGU AUAGGGGCUG ACGCCUGCCC GGUGCUGGAA GGUUAAGAGG AGCGCUUAGC GUAAGCGAAG GUGCGAAUUG AAG CCCCAG UAAACGGCGG CCGUAACUAU AACGGUCCUA AGGUAGCGAA AUUCCUUGUC GGGUAAGUUC CGACCCGCAC GAAA GGCGC AACGAUCUGG GCACUGUCUC AACGAGAGAC UCGGUGAAAU UAUAGUACCU GUGAAGAUGC AGGUUACCCG CGACA GGAC GGAAAGACCC CGUGGAGCUU UACUGCAGCC UGAUAUUGAA UGUUGGUACA GCUUGUACAG GAUAGGUAGG AGCCUU GGA AACCGGAGCG CCAGCUUCGG UGGAGGCAUC GGUGGGAUAC UACCCUGGCU GUAUUGACCU UCUAACCCGC CGCCCUU AU CGGGCGGGGA GACAGUGUCA GGUGGGCAGU UUGACUG(OMG)GG CGGUCGCCUC CUAAAAGGUA ACGGAGGCGC CCAA AGGUU CCCUCAGAAU GGUUGGAAAU CAUUCGCAGA GUGUAAAGGC ACAAGGGAGC UUGACUGCGA GACCUACAAG UCGAG CAGG GACGAAAGUC GGGCUUAGUG AUCCGGUGGU UCCGCAUGGA AGGGCCAUCG CUCAACGGAU AAAAGCUACC CCGGGG AUA ACAGGCUUAU CUCCCCCAAG AGUCCACAUC GACGGGGAGG UUUGGCACCU CG(2MA)UGUCGGC UCAUCGCAUC CUG GGGCUG UAGUCGGUCC CAAGGGUUGG GCUGUUCGCC CAUUAAAGCG GUACGCGAGC UGGGUUCAGA ACGUCGUGAG ACAG UUCGG UCCCUAUCCG UCGCGGGCGC AGGAAAUUUG AGAGGAGCUG UCCUUAGUAC GAGAGGACCG GGAUGGACGC ACCGC UGGU G(PSU)ACCAGUUG UUCUGCCAAG GGCAUCGCUG GGUAGCUAUG UGCGGACGGG AUAAGUGCUG AAAGCAUCUA AG CAUGAAG CCCCCCUCAA GAUGAGAUUU CCCAUUCCGC AAGGAAGUAA GAUCCCUGAA AGAUGAUCAG GUUGAUAGGU CUG AGGUGG AAGUGUGGCG ACACAUGGAG CUGACAGAUA CUAAUCGAUC GAGGACUUAA C

GENBANK: GENBANK: CP010052.1

Macromolecule #8: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 8 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 38.423863 KDa

Sequence

String:

UUUGGUGGCG AUAGCGAAGA GGUCACACCC GUUCCCAUAC CGAACACGGA AGUUAAGCUC UUCAGCGCCG AUGGUAGUCG GGGGUUUCC CCCUGUGAGA GUAGGACGCC GCCAAGCAAG

GENBANK: GENBANK: CP030925.1

Macromolecule #30: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 30 / Number of copies: 1
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 503.369125 KDa

Sequence

String:

UUUAUCGGAG AGUUUGAUCC UGGCUCAGGA CGAACGCUGG CGGCGUGCCU AAUACAUGCA AGUCGAGCGG ACAGAUGGGA GCUUGCUCC CUGAUGUUAG CGGCGGACGG GUGAGUAACA CGUGGGUAAC CUGCCUGUAA GACUGGGAUA ACUCCGGGAA A CCGGGGCU AAUACCGGAU GGUUGUUUGA ACCGCAUGGU UCAAACAUAA AAGGUGGCUU CGGCUACCAC UUACAGAUGG AC CCGCGGC GCAUUAGCUA GUUGGUGAGG UAACGGCUCA CCAAGGCGAC GAUGCGUAGC CGACCUGAGA GGGUGAUCGG CCA CACUGG GACUGAGACA CGGCCCAGAC UCCUACGGGA GGCAGCAGUA GGGAAUCUUC CGCAAUGGAC GAAAGUCUGA CGGA GCAAC GCCGCGUGAG UGAUGAAGGU UUUCGGAUCG UAAAGCUCUG UUGUUAGGGA AGAACAAGUG CCGUUCGAAU AGGGC GGUA CCUUGACGGU ACCUAACCAG AAAGCCACGG CUAACUACGU GCCAGCAGCC GCGGUAAUAC GUAGGUGGCA AGCGUU GUC CGGAAUUAUU GGGCGUAAAG GGCUCGCAGG CGGUUUCUUA AGUCUGAUGU GAAAGCCCCC GGCUCAACCG GGGAGGG UC AUUGGAAACU GGGGAACUUG AGUGCAGAAG AGGAGAGUGG AAUUCCACGU GUAGCGGUGA AAUGCGUAGA GAUGUGGA G GAACACCAGU GGCGAAGGCG ACUCUCUGGU CUGUAACUGA CGCUGAGGAG CGAAAGCGUG GGGAGCGAAC AGGAUUAGA UACCCUGGUA GUCCACGCCG UAAACGAUGA GUGCUAAGUG UUAGGGGGUU UCCGCCCCUU AGUGCUGCAG CUAACGCAUU AAGCACUCC GCCUGGGGAG UACGGUCGCA AGACUGAAAC UCAAAGGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU G UGGUUUAA UUCGAAGCAA CGCGAAGAAC CUUACCAGGU CUUGACAUCC UCUGACAAUC CUAGAGAUAG GACGUCCCCU UC GGGGGCA GAGUGACAGG UGGUGCAUGG UUGUCGUCAG CUCGUGUCGU GAGAUGUUGG GUUAAGUCCC GCAACGAGCG CAA CCCUUG AUCUUAGUUG CCAGCAUUCA GUUGGGCACU CUAAGGUGAC UGCCGGUGAC AAACCGGAGG AAGGUGGGGA UGAC GUCAA AUCAUCAUGC CCCUUAUGAC CUGGGCUACA CACGUGCUAC AAUGGACAGA ACAAAGGGCA GCGAAACCGC GAGGU UAAG CCAAUCCCAC AAAUCUGUUC UCAGUUCGGA UCGCAGUCUG CAACUCGACU GCGUGAAGCU GGAAUCGCUA GUAAUC GCG GAUCAGCAUG CCGCGGUGAA UACGUUCCCG GGCCUUGUAC ACACCGCCCG UCACACCACG AGAGUUUGUA ACACCCG AA GUCGGUGAGG UAACCUUUUA GGAGCCAGCC GCCGAAGGUG GGACAGAUGA UUGGGGUGAA GUCGUAACAA GGUAGCCG U AUCGGAAGGU GCGGCUGGAU CACCUCCUUU CUA

GENBANK: GENBANK: AL009126.3

Macromolecule #37: Pro-tRNA

• Macromolecule: Name: Pro-tRNA / type: rna / ID: 37 / Number of copies: 2
• Source (natural): Organism: Bacillus subtilis subsp. subtilis str. 168 (bacteria)
• Molecular weight: Theoretical: 24.832715 KDa

Sequence

String:

CGGGAAGUAG CUCAGCUUGG UAGAGCACAU GGUUUGGGAC CAUGGGGUCG CAGGUUCGAA UCCUGUCUUC CCGACCA

GENBANK: GENBANK: CP052842.1

Macromolecule #38: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Amycolatopsis japonica (bacteria)
• Molecular weight: Theoretical: 1.788101 KDa

Sequence

String:

CCUCCU

Macromolecule #50: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 50 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #51: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 51 / Number of copies: 199 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #52: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 52 / Number of copies: 43 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #53: PROLINE

• Macromolecule: Name: PROLINE / type: ligand / ID: 53 / Number of copies: 1 / Formula: PRO
• Molecular weight: Theoretical: 115.13 Da

Chemical component information

ChemComp-PRO:
PROLINE

Macromolecule #54: water

• Macromolecule: Name: water / type: ligand / ID: 54 / Number of copies: 1022 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 75.6 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: INSILICO MODEL; In silico model: An unpublished map from our group was initially low-pass filtered and used as model, since the biology was analogous
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 142978
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD