Journal: PLoS Biol / Year: 2011 Title: The Cryo-EM structure of a complete 30S translation initiation complex from Escherichia coli. Authors: Patricia Julián / Pohl Milon / Xabier Agirrezabala / Gorka Lasso / David Gil / Marina V Rodnina / Mikel Valle / Abstract: Formation of the 30S initiation complex (30S IC) is an important checkpoint in regulation of gene expression. The selection of mRNA, correct start codon, and the initiator fMet-tRNA(fMet) requires ...Formation of the 30S initiation complex (30S IC) is an important checkpoint in regulation of gene expression. The selection of mRNA, correct start codon, and the initiator fMet-tRNA(fMet) requires the presence of three initiation factors (IF1, IF2, IF3) of which IF3 and IF1 control the fidelity of the process, while IF2 recruits fMet-tRNA(fMet). Here we present a cryo-EM reconstruction of the complete 30S IC, containing mRNA, fMet-tRNA(fMet), IF1, IF2, and IF3. In the 30S IC, IF2 contacts IF1, the 30S subunit shoulder, and the CCA end of fMet-tRNA(fMet), which occupies a novel P/I position (P/I1). The N-terminal domain of IF3 contacts the tRNA, whereas the C-terminal domain is bound to the platform of the 30S subunit. Binding of initiation factors and fMet-tRNA(fMet) induces a rotation of the head relative to the body of the 30S subunit, which is likely to prevail through 50S subunit joining until GTP hydrolysis and dissociation of IF2 take place. The structure provides insights into the mechanism of mRNA selection during translation initiation.
History
Deposition
Aug 3, 2010
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Header (metadata) release
Sep 16, 2011
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Map release
Sep 16, 2011
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Update
Dec 11, 2013
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Current status
Dec 11, 2013
Processing site: PDBe / Status: Released
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