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Open data
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Basic information
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Title | RPA tetrameric supercomplex from Pyrococcus abyssi | |||||||||
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![]() | DNA replication / single strand DNA-binding protein / RPA / DNA BINDING PROTEIN | |||||||||
Function / homology | ![]() response to ionizing radiation / double-strand break repair via homologous recombination / chromosome, telomeric region / nucleic acid binding / DNA binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.35 Å | |||||||||
![]() | Madru C / Martinez-Carranza M / Legrand P / Sauguet L | |||||||||
Funding support | ![]()
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![]() | ![]() Title: DNA-binding mechanism and evolution of replication protein A. Authors: Clément Madru / Markel Martínez-Carranza / Sébastien Laurent / Alessandra C Alberti / Maelenn Chevreuil / Bertrand Raynal / Ahmed Haouz / Rémy A Le Meur / Marc Delarue / Ghislaine ...Authors: Clément Madru / Markel Martínez-Carranza / Sébastien Laurent / Alessandra C Alberti / Maelenn Chevreuil / Bertrand Raynal / Ahmed Haouz / Rémy A Le Meur / Marc Delarue / Ghislaine Henneke / Didier Flament / Mart Krupovic / Pierre Legrand / Ludovic Sauguet / ![]() Abstract: Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in ...Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integrative structural, biochemical and biophysical approach, we extensively characterize RPA from Pyrococcus abyssi in the presence and absence of DNA. The obtained X-ray and cryo-EM structures reveal that the trimerization core and interactions promoting RPA clustering on ssDNA are shared between archaea and eukaryotes. However, we also identified a helical domain named AROD (Acidic Rpa1 OB-binding Domain), and showed that, in Archaea, RPA forms an unanticipated tetrameric supercomplex in the absence of DNA. The four RPA molecules clustered within the tetramer could efficiently coat and protect stretches of ssDNA created by the advancing replisome. Finally, our results provide insights into the evolution of this primordial replication factor in eukaryotes. | |||||||||
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | 203.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.3 KB 18.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.7 KB | Display | ![]() |
Images | ![]() | 83 KB | ||
Masks | ![]() | 216 MB | ![]() | |
Filedesc metadata | ![]() | 5.8 KB | ||
Others | ![]() ![]() | 200 MB 200 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8c5yMC ![]() 8aa9C ![]() 8aajC ![]() 8aasC ![]() 8c5zC ![]() 8oejC ![]() 8oelC ![]() 15300 M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.86 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Half map: #1
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-Half map: #2
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Sample components
-Entire : RPA tetrameric supercomplex
Entire | Name: RPA tetrameric supercomplex |
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Components |
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-Supramolecule #1: RPA tetrameric supercomplex
Supramolecule | Name: RPA tetrameric supercomplex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 345 KDa |
-Macromolecule #1: Replication factor A
Macromolecule | Name: Replication factor A / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 20.234057 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: VATYTRKKIK DIEAGDRFVE VRGTIAKVYR VLTYDACPEC KKKVDYDEGL GVWICPEHGE VQPIKMTILD FGLDDGTGYI RVTLFGDDA EELLGVSPEE IAEKIKELEE SGLTTKEAAR KLAEDEFYNI IGREIVVRGN VIEDRFLGLI LRASSWEDVD Y RREIERIK EELEKLGVM UniProtKB: Replication factor A |
-Macromolecule #2: RPA32 subunit of the hetero-oligomeric complex involved in homolo...
Macromolecule | Name: RPA32 subunit of the hetero-oligomeric complex involved in homologous recombination type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 20.930475 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: KKRMPATRLY IKDILEGYFV KSEGDFEPNY LITKYARKVY RAKIVGTVVR EPLIAEDETY GKFQVDDGTG VIWVLGFRDD TKFAKLVRK GDLVQVIGKI AEWRDDKQIL VEGVSKVHPN MWILHRYETL KEKIEHIKKA KIALEIYNQY GITAKSKVIA K NKGIEEEL LEVIDELYGI MM UniProtKB: RPA32 subunit of the hetero-oligomeric complex involved in homologous recombination |
-Macromolecule #3: RPA14 subunit of the hetero-oligomeric complex involved in homolo...
Macromolecule | Name: RPA14 subunit of the hetero-oligomeric complex involved in homologous recombination type: protein_or_peptide / ID: 3 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 13.078994 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: RRRKPAVERK ISEIREEDTR VSLIGRVIKV DKMDYMFWLD DGTGVAIIES ESDLPKVGQV VRVIGRIIRN EEGIHIYAEV IQDFSDADL EALEEIRELE RKLLPRLEGE IVW UniProtKB: RPA14 subunit of the hetero-oligomeric complex involved in homologous recombination |
-Macromolecule #4: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 4 / Number of copies: 4 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |