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- EMDB-16408: Human mitochondrial ribosome in complex with LRPPRC-SLIRP, A-site... -
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Open data
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Basic information
Entry | ![]() | ||||||||||||||||||
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Title | Human mitochondrial ribosome in complex with LRPPRC-SLIRP, A-site, P-site, E-site tRNAs and mRNA (focussed on SSU body) | ||||||||||||||||||
![]() | Local resolution filtered map masked refined on SSU body, fitted | ||||||||||||||||||
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![]() | mitochondrial translation / mRNA delivery / RIBOSOME | ||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.89 Å | ||||||||||||||||||
![]() | Singh V / Itoh Y / Amunts A | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of LRPPRC-SLIRP-dependent translation by the mitoribosome Authors: Singh V / Conor J / Itoh Y / Soto T / Fontanesi F / Couvillian M / Huynen M / Churchman S / Barrientos A / Amunts A | ||||||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 559 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.4 KB 19.4 KB | Display Display | ![]() |
Images | ![]() | 69.8 KB | ||
Filedesc metadata | ![]() | 4.1 KB | ||
Others | ![]() ![]() ![]() ![]() | 907.5 MB 816.3 MB 816.9 MB 816.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Local resolution filtered map masked refined on SSU body, fitted | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Unsharpened map masked refined on SSU body, fitted
File | emd_16408_additional_1.map | ||||||||||||
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Annotation | Unsharpened map masked refined on SSU body, fitted | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Raw unsharpened map masked refined on SSU body
File | emd_16408_additional_2.map | ||||||||||||
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Annotation | Raw unsharpened map masked refined on SSU body | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Raw half map masked refined on SSU body
File | emd_16408_half_map_1.map | ||||||||||||
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Annotation | Raw half map masked refined on SSU body | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Raw half map masked refined on SSU body
File | emd_16408_half_map_2.map | ||||||||||||
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Annotation | Raw half map masked refined on SSU body | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Human mitochondrial ribosome bound to LRPPRC-SLIRP, mRNA and tRNA...
Entire | Name: Human mitochondrial ribosome bound to LRPPRC-SLIRP, mRNA and tRNAs in the A-site, P-site and E-site |
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Components |
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-Supramolecule #1: Human mitochondrial ribosome bound to LRPPRC-SLIRP, mRNA and tRNA...
Supramolecule | Name: Human mitochondrial ribosome bound to LRPPRC-SLIRP, mRNA and tRNAs in the A-site, P-site and E-site type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.6 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 41815 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |