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- EMDB-15468: In situ subtomogram average of floating microtubule inner protein... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | In situ subtomogram average of floating microtubule inner protein 16 (fMIP16) | |||||||||
![]() | In situ subtomogram average of floating microtubule inner protein 16 | |||||||||
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![]() | microtubule associated protein / STRUCTURAL PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 27.0 Å | |||||||||
![]() | Chakraborty S / Mahamid J / Martinez-Sanchez A / Baumeister W | |||||||||
Funding support | 1 items
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![]() | ![]() Title: In situ subtomogram average of floating microtubule inner protein 16 (fMIP16) Authors: Chakraborty S / Mahamid J / Martinez-Sanchez A / Baumeister W | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 2.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.5 KB 14.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 4.7 KB | Display | ![]() |
Images | ![]() | 9.3 KB | ||
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 6 MB 6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 836 KB | Display | ![]() |
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Full document | ![]() | 835.6 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 13.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 16 | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.129 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: In situ subtomogram average of floating microtubule inner protein 16
File | emd_15468_half_map_1.map | ||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 16 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: In situ subtomogram average of floating microtubule inner protein 16
File | emd_15468_half_map_2.map | ||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 16 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : fMIP16 from human induced pluripotent stem cells derived neuronal...
Entire | Name: fMIP16 from human induced pluripotent stem cells derived neuronal microtubules |
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Components |
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-Supramolecule #1: fMIP16 from human induced pluripotent stem cells derived neuronal...
Supramolecule | Name: fMIP16 from human induced pluripotent stem cells derived neuronal microtubules type: cell / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.2 |
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Grid | Model: Quantifoil / Material: GOLD / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. |
Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 70 % / Chamber temperature: 310 K / Instrument: LEICA EM GP |
Details | Frozen hydrated human induced pluripotent stem cells derived neurons |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 77.0 K |
Specialist optics | Phase plate: VOLTA PHASE PLATE / Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 2.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 2.5 µm / Nominal magnification: 64000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |