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- EMDB-15436: In situ subtomogram average of floating microtubule inner protein... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | In situ subtomogram average of floating microtubule inner protein 3 (fMIP3) | |||||||||
![]() | In situ subtomogram average of floating microtubule inner protein 3 | |||||||||
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![]() | Microtubule-associated protein / STRUCTURAL PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 27.0 Å | |||||||||
![]() | Chakraborty S / Martinez-Sanchez A / Baumeister W / Mahamid J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: In situ subtomogram average of floating microtubule inner protein 3 (fMIP3) Authors: Chakraborty S / Martinez-Sanchez A / Baumeister W / Mahamid J | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 450.6 KB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.6 KB 15.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 4.7 KB | Display | ![]() |
Images | ![]() | 8 KB | ||
Filedesc metadata | ![]() | 4.2 KB | ||
Others | ![]() ![]() | 6 MB 6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 777.3 KB | Display | ![]() |
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Full document | ![]() | 776.8 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 14 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 3 | ||||||||||||||||||||
Voxel size | X=Y=Z: 3.42 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: In situ subtomogram average of floating microtubule inner protein 3
File | emd_15436_half_map_1.map | ||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 3 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: In situ subtomogram average of floating microtubule inner protein 3
File | emd_15436_half_map_2.map | ||||||||||||
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Annotation | In situ subtomogram average of floating microtubule inner protein 3 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Floating microtubule inner protein 3 (fMIP3)
Entire | Name: Floating microtubule inner protein 3 (fMIP3) |
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Components |
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-Supramolecule #1: Floating microtubule inner protein 3 (fMIP3)
Supramolecule | Name: Floating microtubule inner protein 3 (fMIP3) / type: cell / ID: 1 / Parent: 0 / Details: Pluripotent P19 cells cultured on EM grid |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.2 |
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Grid | Model: Quantifoil R2/1 / Material: GOLD / Mesh: 200 / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. |
Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 95 % / Chamber temperature: 310 K / Instrument: FEI VITROBOT MARK IV Details: blotted from backside with blot force 10, blot time 10 seconds.. |
Details | frozen hydrated pluripotent P19 cells |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 195.0 K |
Specialist optics | Phase plate: VOLTA PHASE PLATE / Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.4 e/Å2 / Details: bidirectional tilting scheme |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 0.5 µm / Nominal defocus min: 0.2 µm / Nominal magnification: 42000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |