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- EMDB-15443: In situ cryo-electron tomogram of a Taxol treated intact pluripot... -
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Open data
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Basic information
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Title | In situ cryo-electron tomogram of a Taxol treated intact pluripotent P19 cell. | |||||||||
![]() | In situ cryo-electron tomogram of a Taxol treated intact pluripotent P19 cell. | |||||||||
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![]() | Microtubules / Scaffold / protein transport | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Chakraborty S / Martinez-Sanchez A / Baumeister W / Mahamid J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-electron tomography suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules Authors: Chakraborty S / Martinez-Sanchez A / Beck F / Toro-Nahuelpan M / Hwang IY / Noh KM / Baumeister W / Mahamid J | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 34.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.2 KB 9.2 KB | Display Display | ![]() |
Images | ![]() | 260.4 KB | ||
Filedesc metadata | ![]() | 3.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 606.9 KB | Display | ![]() |
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Full document | ![]() | 606.5 KB | Display | |
Data in XML | ![]() | 4 KB | Display | |
Data in CIF | ![]() | 4.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||
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Annotation | In situ cryo-electron tomogram of a Taxol treated intact pluripotent P19 cell. | ||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. generated in cubic-lattice coordinate | ||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 27.36 Å | ||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : In situ cryo-electron tomogram of a Taxol treated intact pluripot...
Entire | Name: In situ cryo-electron tomogram of a Taxol treated intact pluripotent P19 cell. |
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Components |
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-Supramolecule #1: In situ cryo-electron tomogram of a Taxol treated intact pluripot...
Supramolecule | Name: In situ cryo-electron tomogram of a Taxol treated intact pluripotent P19 cell. type: cell / ID: 1 / Parent: 0 Details: The cells were treated with Taxol for 30 minutes prior to vitrification |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.2 |
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Grid | Model: Quantifoil R2/1 / Material: GOLD / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. |
Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 95 % / Chamber temperature: 310 K / Instrument: FEI VITROBOT MARK IV Details: blot force 10, blot time 10 seconds from backside.. |
Details | Cells are cultured on EM grid |
Sectioning | Other: NO SECTIONING |
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Electron microscopy
Microscope | TFS KRIOS |
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Temperature | Min: 77.0 K |
Specialist optics | Phase plate: VOLTA PHASE PLATE / Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 0.5 µm / Nominal defocus min: 0.2 µm / Nominal magnification: 42000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Algorithm: BACK PROJECTION / Software - Name: ![]() |
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