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- EMDB-1467: Human Parvovirus B19 (empty wildtype particle) -

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Basic information

Entry
Database: EMDB / ID: 1467
TitleHuman Parvovirus B19 (empty wildtype particle)
Map dataHuman Parvovirus B19 (eB19, empty wildtype particle)
SampleHuman Parvovirus B19 (empty wildtype particle):
virus
Keywordsicosahedral / empty viral particle / B19
SourceHuman parvovirus B19 (Human Parvovirus B19)
Methodsingle particle reconstruction / cryo EM / 11.3 Å resolution
AuthorsKaufmann B / Chipman PR / Modrow S / Rossmann MG
CitationJournal: J. Virol. / Year: 2008
Title: Visualization of the externalized VP2 N termini of infectious human parvovirus B19.
Authors: Bärbel Kaufmann / Paul R Chipman / Victor A Kostyuchenko / Susanne Modrow / Michael G Rossmann
DateDeposition: Feb 5, 2008 / Header (metadata) release: Feb 7, 2008 / Map release: Mar 31, 2009 / Last update: Mar 31, 2009

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 18
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 18
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_1467.map.gz (map file in CCP4 format, 5344 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)X (Row.)Y (Col.)
111 pix
2.82 Å/pix.
= 312.63 Å
111 pix
2.82 Å/pix.
= 312.63 Å
111 pix
2.82 Å/pix.
= 312.63 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.81649 Å
Density
Contour Level:11, 18 (movie #1):
Minimum - Maximum-10.31 - 34.561
Average (Standard dev.)2.24292 (8.7631)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderYXZ
Dimensions111111111
Origin-55-55-55
Limit555555
Spacing111111111
CellA=B=C: 312.63 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.81648648648652.81648648648652.8164864864865
M x/y/z111111111
origin x/y/z0.0000.0000.000
length x/y/z312.630312.630312.630
α/β/γ90.00090.00090.000
start NX/NY/NZ-55-55-55
NX/NY/NZ111111111
MAP C/R/S213
start NC/NR/NS-55-55-55
NC/NR/NS111111111
D min/max/mean-10.31034.5612.243

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Supplemental data

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Sample components

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Entire Human Parvovirus B19 (empty wildtype particle)

EntireName: Human Parvovirus B19 (empty wildtype particle)
Details: icosahedral, empty viral particle, non-enveloped protein shell
Number of components: 1
Oligomeric State: 60 VP subunits form icosahedral protein shell
MassTheoretical: 3.6 MDa

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Component #1: virus, Human parvovirus B19

VirusName: Human parvovirus B19 / a.k.a: Human Parvovirus B19 / Class: OTHER
Details: empty wildtype particles purified from human plasma, no ssDNA genome encapsidated
Empty: Yes / Enveloped: No / Isolate: SPECIES
MassTheoretical: 3.6 MDa
SpeciesSpecies: Human parvovirus B19 (Human Parvovirus B19)
Source (natural)Host Species: Homo sapiens (human) / Host category: VERTEBRATES

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionSpecimen conc.: 1 mg/ml / Buffer solution: 25mM Tris-HCl / pH: 7.5
Support film400 mesh copper
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: ETHANE
Method: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.
Details: Vitrification instrument: guillotine-style plunge freezing device

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Electron microscopy imaging

ImagingMicroscope: FEI/PHILIPS CM300FEG/T / Details: low dose
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 22 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 45000 X (nominal), 47000 X (calibrated) / Astigmatism: live FFT at 200K / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 1250 - 3660 nm
Specimen HolderHolder: Eucentric / Model: GATAN LIQUID NITROGEN / Temperature: 98 K
CameraDetector: KODAK SO-163 FILM

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Image acquisition

Image acquisitionNumber of digital images: 31 / Scanner: ZEISS SCAI / Sampling size: 14 microns / Bit depth: 8

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Image processing

ProcessingMethod: single particle reconstruction / Details: manual particle selection / Number of projections: 1959 / Applied symmetry: I (icosahedral)
3D reconstructionAlgorithm: model-based approach using Fourier methods / Software: EMPFT, POR, P3DR / CTF correction: each particle
Details: final map includes data to 10.5 Ang resolution (fsc 0.3 cut-off), magnification of final map standardized to a map calculated from B19 VP2 VLP model coordinates (PDB accession no 1S58) resulting in final pixel separation of 2.8165Ang
Resolution: 11.3 Å / Resolution method: FSC 0.5

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