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- EMDB-13537: S. cerevisiae replisome-SCF(Dia2) complex bound to double-strande... -

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基本情報

登録情報
データベース: EMDB / ID: EMD-13537
タイトルS. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
マップデータComposite cryo-EM density map for the budding yeast CMG-Csm3-Tof1-Mrc1-Ctf4-PolE-SCF(Dia2) complex on double-stranded DNA (conformation I), produced using the Phenix combine_focused_maps progam
試料
  • 複合体: Budding yeast replisome on double-stranded DNA engaged with SCF(Dia2) (conformation I)
    • 複合体: DNA
      • DNA: x 2種
    • 複合体: Replisome
      • タンパク質・ペプチド: x 16種
    • タンパク質・ペプチド: x 2種
  • リガンド: x 3種
キーワードGenome stability / DNA replication / Ubiquitination / termination / replisome / cryo-EM / CMG / SCF(Dia2) / REPLICATION
機能・相同性
機能・相同性情報


: / cell cycle / mitotic DNA replication termination / DNA-templated DNA replication maintenance of fidelity / gene conversion / Unwinding of DNA / maintenance of DNA repeat elements / invasive growth in response to glucose limitation / DNA replication initiation / replication fork arrest ...: / cell cycle / mitotic DNA replication termination / DNA-templated DNA replication maintenance of fidelity / gene conversion / Unwinding of DNA / maintenance of DNA repeat elements / invasive growth in response to glucose limitation / DNA replication initiation / replication fork arrest / meiotic chromosome segregation / protein-containing complex disassembly / epsilon DNA polymerase complex / DNA strand elongation involved in mitotic DNA replication / MCM core complex / Assembly of the pre-replicative complex / Switching of origins to a post-replicative state / GINS complex / MCM complex binding / mitotic DNA replication preinitiation complex assembly / nuclear DNA replication / premeiotic DNA replication / SUMO binding / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / nucleotide-excision repair, DNA gap filling / mitotic DNA replication / Activation of the pre-replicative complex / DNA replication checkpoint signaling / CMG complex / establishment of mitotic sister chromatid cohesion / nuclear pre-replicative complex / DNA replication proofreading / Termination of translesion DNA synthesis / Activation of ATR in response to replication stress / DNA replication preinitiation complex / single-stranded DNA 3'-5' DNA exonuclease activity / MCM complex / replication fork protection complex / mitotic DNA replication checkpoint signaling / double-strand break repair via break-induced replication / single-stranded DNA helicase activity / mitotic DNA replication initiation / mitotic intra-S DNA damage checkpoint signaling / silent mating-type cassette heterochromatin formation / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドデオキシリボヌクレアーゼ / SCF ubiquitin ligase complex / 3'-5' DNA helicase activity / mitotic sister chromatid cohesion / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / DNA strand elongation involved in DNA replication / leading strand elongation / replication fork processing / nuclear replication fork / regulation of DNA replication / cullin family protein binding / DNA replication origin binding / Dual incision in TC-NER / DNA replication initiation / error-prone translesion synthesis / subtelomeric heterochromatin formation / DNA helicase activity / base-excision repair, gap-filling / replication fork / meiotic cell cycle / helicase activity / base-excision repair / double-strand break repair via nonhomologous end joining / DNA-templated DNA replication / double-strand break repair / mitotic cell cycle / single-stranded DNA binding / 4 iron, 4 sulfur cluster binding / double-stranded DNA binding / ubiquitin-dependent protein catabolic process / DNA helicase / DNA-directed DNA polymerase / forked DNA-dependent helicase activity / single-stranded 3'-5' DNA helicase activity / four-way junction helicase activity / double-stranded DNA helicase activity / DNA-directed DNA polymerase activity / chromosome, telomeric region / DNA replication / hydrolase activity / protein ubiquitination / DNA repair / nucleotide binding / mRNA binding / chromatin binding / ATP hydrolysis activity / DNA binding / zinc ion binding / nucleoplasm / ATP binding / metal ion binding / nucleus / cytosol / cytoplasm
類似検索 - 分子機能
F-box domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / DNA polymerase epsilon, subunit B / Timeless, N-terminal / Timeless / Timeless protein / Minichromosome loss protein Mcl1, middle region / Minichromosome loss protein, Mcl1, middle region ...F-box domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / DNA polymerase epsilon, subunit B / Timeless, N-terminal / Timeless / Timeless protein / Minichromosome loss protein Mcl1, middle region / Minichromosome loss protein, Mcl1, middle region / Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / : / : / DNA polymerase epsilon catalytic subunit A, thumb domain / Zinc finger domain of DNA polymerase-epsilon / DNA polymerase epsilon, catalytic subunit A, C-terminal / DNA polymerase epsilon catalytic subunit / Domain of unknown function (DUF1744) / DUF1744 / CDC45 family / DNA replication complex GINS protein Psf2 / CDC45 / A Receptor for Ubiquitination Targets / GINS complex, subunit Psf3 / DNA replication complex GINS protein SLD5, C-terminal / GINS complex, subunit Psf3 superfamily / GINS complex protein Sld5, alpha-helical domain / DNA replication complex GINS protein SLD5 C-terminus / GINS complex, subunit Psf1 / GINS complex subunit Sld5 / : / MCM3 winged helix domain / GINS subunit, domain A / GINS, helical bundle-like domain superfamily / GINS complex protein helical bundle domain / F-box domain profile. / MCM4, winged helix domain / F-box-like domain superfamily / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / DNA polymerase alpha/delta/epsilon, subunit B / DNA polymerase alpha/epsilon subunit B / F-box domain / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / MCM3-like, winged helix domain / DNA replication licensing factor Mcm6 / DNA replication licensing factor Mcm7 / Mcm6, C-terminal winged-helix domain / MCM6 C-terminal winged-helix domain / DNA replication licensing factor Mcm2 / Mini-chromosome maintenance protein 2 / Mini-chromosome maintenance, conserved site / MCM family signature. / MCM N-terminal domain / MCM N-terminal domain / MCM OB domain / MCM OB domain / Mini-chromosome maintenance protein / MCM, AAA-lid domain / MCM P-loop domain / MCM AAA-lid domain / MCM family domain profile. / minichromosome maintenance proteins / MCM domain / DNA polymerase family B, thumb domain / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA polymerase, palm domain superfamily / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / TPR repeat region circular profile. / Leucine-rich repeat profile. / SKP1/BTB/POZ domain superfamily / Leucine-rich repeat / Leucine-rich repeat domain superfamily / Tetratricopeptide-like helical domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / WD40-repeat-containing domain superfamily / Winged helix-like DNA-binding domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Nucleic acid-binding, OB-fold / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
類似検索 - ドメイン・相同性
DNA replication licensing factor MCM5 / DNA replication complex GINS protein PSF2 / DNA replication licensing factor MCM2 / DNA replication complex GINS protein PSF1 / E3 ubiquitin ligase complex SCF subunit / DNA replication licensing factor MCM7 / DNA polymerase alpha-binding protein / DNA replication complex GINS protein PSF3 / DNA polymerase epsilon catalytic subunit A / DNA replication licensing factor MCM3 ...DNA replication licensing factor MCM5 / DNA replication complex GINS protein PSF2 / DNA replication licensing factor MCM2 / DNA replication complex GINS protein PSF1 / E3 ubiquitin ligase complex SCF subunit / DNA replication licensing factor MCM7 / DNA polymerase alpha-binding protein / DNA replication complex GINS protein PSF3 / DNA polymerase epsilon catalytic subunit A / DNA replication licensing factor MCM3 / DNA polymerase epsilon subunit B / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM6 / Topoisomerase 1-associated factor 1 / DNA replication complex GINS protein SLD5 / Chromosome segregation in meiosis protein 3 / Cell division control protein 45 / Protein DIA2
類似検索 - 構成要素
生物種DNA molecule (その他) / Saccharomyces cerevisiae (パン酵母) / Synthetic construct (人工物)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å
データ登録者Jenkyn-Bedford M / Yeeles JTP
資金援助 英国, 3件
OrganizationGrant number国
Medical Research Council (MRC, United Kingdom)MC_UP_1201/12 英国
Medical Research Council (MRC, United Kingdom)MC_UU_12016/13 英国
Wellcome Trust204678/Z/16/Z 英国
引用ジャーナル: Nature / 年: 2021
タイトル: A conserved mechanism for regulating replisome disassembly in eukaryotes.
著者: Michael Jenkyn-Bedford / Morgan L Jones / Yasemin Baris / Karim P M Labib / Giuseppe Cannone / Joseph T P Yeeles / Tom D Deegan /
要旨: Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily ...Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily diverse E3 ubiquitin ligases in different eukaryotes (SCF in budding yeast, CUL2 in metazoa), replisome disassembly is governed by a common regulatory principle, in which ubiquitylation of CMG is suppressed before replication termination, to prevent replication fork collapse. Recent evidence suggests that this suppression is mediated by replication fork DNA. However, it is unknown how SCF and CUL2 discriminate terminated from elongating replisomes, to selectively ubiquitylate CMG only after termination. Here we used cryo-electron microscopy to solve high-resolution structures of budding yeast and human replisome-E3 ligase assemblies. Our structures show that the leucine-rich repeat domains of Dia2 and LRR1 are structurally distinct, but bind to a common site on CMG, including the MCM3 and MCM5 zinc-finger domains. The LRR-MCM interaction is essential for replisome disassembly and, crucially, is occluded by the excluded DNA strand at replication forks, establishing the structural basis for the suppression of CMG ubiquitylation before termination. Our results elucidate a conserved mechanism for the regulation of replisome disassembly in eukaryotes, and reveal a previously unanticipated role for DNA in preserving replisome integrity.
履歴
登録2021年9月2日-
ヘッダ(付随情報) 公開2021年11月10日-
マップ公開2021年11月10日-
更新2024年7月17日-
現状2024年7月17日処理サイト: PDBe / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 7.09
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 7.09
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-7pmk
  • 表面レベル: 7.09
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_13537.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_13537.map.gz / 形式: CCP4 / 大きさ: 202.8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Composite cryo-EM density map for the budding yeast CMG-Csm3-Tof1-Mrc1-Ctf4-PolE-SCF(Dia2) complex on double-stranded DNA (conformation I), produced using the Phenix combine_focused_maps progam
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸X (Sec.)Y (Row.)Z (Col.)
1.06 Å/pix.
x 376 pix.
= 398.56 Å		1.06 Å/pix.
x 376 pix.
= 398.56 Å		1.06 Å/pix.
x 376 pix.
= 398.56 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 1.06 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 7.09 / ムービー #1: 7.09
最小 - 最大-40.891254000000004 - 72.2577
平均 (標準偏差)0.04789695 (±1.6774017)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderZYX
Origin000
サイズ376376376
Spacing376376376
セルA=B=C: 398.55997 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z376376376
origin x/y/z0.0000.0000.000
length x/y/z398.560398.560398.560
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ376376376
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS376376376
D min/max/mean-40.89172.2580.048

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添付データ

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試料の構成要素

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全体 : Budding yeast replisome on double-stranded DNA engaged with SCF(D...

全体名称: Budding yeast replisome on double-stranded DNA engaged with SCF(Dia2) (conformation I)
要素
  • 複合体: Budding yeast replisome on double-stranded DNA engaged with SCF(Dia2) (conformation I)
    • 複合体: DNA
      • DNA: Leading strand template DNA
      • DNA: Lagging strand template DNA
    • 複合体: Replisome
      • タンパク質・ペプチド: DNA replication licensing factor MCM2
      • タンパク質・ペプチド: DNA replication licensing factor MCM3
      • タンパク質・ペプチド: DNA replication licensing factor MCM4
      • タンパク質・ペプチド: DNA helicase
      • タンパク質・ペプチド: DNA replication licensing factor MCM6
      • タンパク質・ペプチド: DNA replication licensing factor MCM7
      • タンパク質・ペプチド: DNA replication complex GINS protein PSF1
      • タンパク質・ペプチド: DNA replication complex GINS protein PSF2
      • タンパク質・ペプチド: DNA replication complex GINS protein PSF3
      • タンパク質・ペプチド: DNA replication complex GINS protein SLD5
      • タンパク質・ペプチド: Cell division control protein 45,Cell division control protein 45
      • タンパク質・ペプチド: DNA polymerase alpha-binding protein
      • タンパク質・ペプチド: DNA polymerase epsilon catalytic subunit A
      • タンパク質・ペプチド: DNA polymerase epsilon subunit B
      • タンパク質・ペプチド: Topoisomerase 1-associated factor 1
      • タンパク質・ペプチド: Chromosome segregation in meiosis protein 3
    • タンパク質・ペプチド: E3 ubiquitin ligase complex SCF subunit
    • タンパク質・ペプチド: Protein DIA2
  • リガンド: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • リガンド: MAGNESIUM ION
  • リガンド: ZINC ION

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超分子 #1: Budding yeast replisome on double-stranded DNA engaged with SCF(D...

超分子名称: Budding yeast replisome on double-stranded DNA engaged with SCF(Dia2) (conformation I)
タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#20
詳細: Complex reconstituted in vitro from purified proteins and DNA

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超分子 #2: DNA

超分子名称: DNA / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #13-#14
由来(天然)生物種: DNA molecule (その他)

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超分子 #3: Replisome

超分子名称: Replisome / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #1-#12, #17-#20
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)

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分子 #1: DNA replication licensing factor MCM2

分子名称: DNA replication licensing factor MCM2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 98.911539 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD IDEVEEQMNE VDLMDDNMYE DYAADHNRD RYDPDQVDDR EQQELSLSER RRIDAQLNER DRLLRNVAYI DDEDEEQEGA AQLDEMGLPV QRRRRRRQYE D LENSDDDL ...文字列:
MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD IDEVEEQMNE VDLMDDNMYE DYAADHNRD RYDPDQVDDR EQQELSLSER RRIDAQLNER DRLLRNVAYI DDEDEEQEGA AQLDEMGLPV QRRRRRRQYE D LENSDDDL LSDMDIDPLR EELTLESLSN VKANSYSEWI TQPNVSRTIA RELKSFLLEY TDETGRSVYG ARIRTLGEMN SE SLEVNYR HLAESKAILA LFLAKCPEEM LKIFDLVAME ATELHYPDYA RIHSEIHVRI SDFPTIYSLR ELRESNLSSL VRV TGVVTR RTGVFPQLKY VKFNCLKCGS ILGPFFQDSN EEIRISFCTN CKSKGPFRVN GEKTVYRNYQ RVTLQEAPGT VPPG RLPRH REVILLADLV DVSKPGEEVE VTGIYKNNYD GNLNAKNGFP VFATIIEANS IKRREGNTAN EGEEGLDVFS WTEEE EREF RKISRDRGII DKIISSMAPS IYGHRDIKTA VACSLFGGVP KNVNGKHSIR GDINVLLLGD PGTAKSQILK YVEKTA HRA VFATGQGASA VGLTASVRKD PITKEWTLEG GALVLADKGV CLIDEFDKMN DQDRTSIHEA MEQQSISISK AGIVTTL QA RCSIIAAANP NGGRYNSTLP LAQNVSLTEP ILSRFDILCV VRDLVDEEAD ERLATFVVDS HVRSHPENDE DREGEELK N NGESAIEQGE DEINEQLNAR QRRLQRQRKK EEEISPIPQE LLMKYIHYAR TKIYPKLHQM DMDKVSRVYA DLRRESIST GSFPITVRHL ESILRIAESF AKMRLSEFVS SYDLDRAIKV VVDSFVDAQK VSVRRQLRRS FAIYTLGH

UniProtKB: DNA replication licensing factor MCM2

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分子 #2: DNA replication licensing factor MCM3

分子名称: DNA replication licensing factor MCM3 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 111.987562 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEAPVME GSTGFDGDAT TFFAPDAVFG DRVRRFQEFL DTFTSYRDSV RSIQVYNSN NAANYNDDQD DADERDLLGD DDGDDLEKEK KAASSTSLNI LPHRIIISLD DLREFDRSFW SGILVEPAYF I PPAEKALT ...文字列:
MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEAPVME GSTGFDGDAT TFFAPDAVFG DRVRRFQEFL DTFTSYRDSV RSIQVYNSN NAANYNDDQD DADERDLLGD DDGDDLEKEK KAASSTSLNI LPHRIIISLD DLREFDRSFW SGILVEPAYF I PPAEKALT DLADSMDDVP HPNASAVSSR HPWKLSFKGS FGAHALSPRT LTAQHLNKLV SVEGIVTKTS LVRPKLIRSV HY AAKTGRF HYRDYTDATT TLTTRIPTPA IYPTEDTEGN KLTTEYGYST FIDHQRITVQ EMPEMAPAGQ LPRSIDVILD DDL VDKTKP GDRVNVVGVF KSLGAGGMNQ SNSNTLIGFK TLILGNTVYP LHARSTGVAA RQMLTDFDIR NINKLSKKKD IFDI LSQSL APSIYGHDHI KKAILLMLMG GVEKNLENGS HLRGDINILM VGDPSTAKSQ LLRFVLNTAS LAIATTGRGS SGVGL TAAV TTDRETGERR LEAGAMVLAD RGVVCIDEFD KMTDVDRVAI HEVMEQQTVT IAKAGIHTTL NARCSVIAAA NPVFGQ YDV NRDPHQNIAL PDSLLSRFDL LFVVTDDINE IRDRSISEHV LRTHRYLPPG YLEGEPVRER LNLSLAVGED ADINPEE HS NSGAGVENEG EDDEDHVFEK FNPLLQAGAK LAKNKGNYNG TEIPKLVTIP FLRKYVQYAK ERVIPQLTQE AINVIVKN Y TDLRNDDNTK KSPITARTLE TLIRLATAHA KVRLSKTVNK VDAKVAANLL RFALLGEDIG NDIDEEESEY EEALSKRSP QKSPKKRQRV RQPASNSGSP IKSTPRRSTA SSVNATPSSA RRILRFQDDE QNAGEDDNDI MSPLPADEEA ELQRRLQLGL RVSPRRREH LHAPEEGSSG PLTEVGTPRL PNVSSAGQDD EQQQSVISFD NVEPGTISTG RLSLISGIIA RLMQTEIFEE E SYPVASLF ERINEELPEE EKFSAQEYLA GLKIMSDRNN LMVADDKVWR V

UniProtKB: DNA replication licensing factor MCM3

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分子 #3: DNA replication licensing factor MCM4

分子名称: DNA replication licensing factor MCM4 / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 105.138375 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQG RQGRIRSSAS ASGRSRYHSD LRSDRALPTS SSSLGRNGQN RVHMRRNDIH TSDLSSPRRI VDFDTRSGVN T LDTSSSSA ...文字列:
MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQG RQGRIRSSAS ASGRSRYHSD LRSDRALPTS SSSLGRNGQN RVHMRRNDIH TSDLSSPRRI VDFDTRSGVN T LDTSSSSA PPSEASEPLR IIWGTNVSIQ ECTTNFRNFL MSFKYKFRKI LDEREEFINN TTDEELYYIK QLNEMRELGT SN LNLDARN LLAYKQTEDL YHQLLNYPQE VISIMDQTIK DCMVSLIVDN NLDYDLDEIE TKFYKVRPYN VGSCKGMREL NPN DIDKLI NLKGLVLRST PVIPDMKVAF FKCNVCDHTM AVEIDRGVIQ EPARCERIDC NEPNSMSLIH NRCSFADKQV IKLQ ETPDF VPDGQTPHSI SLCVYDELVD SCRAGDRIEV TGTFRSIPIR ANSRQRVLKS LYKTYVDVVH VKKVSDKRLD VDTST IEQE LMQNKVDHNE VEEVRQITDQ DLAKIREVAA REDLYSLLAR SIAPSIYELE DVKKGILLQL FGGTNKTFTK GGRYRG DIN ILLCGDPSTS KSQILQYVHK ITPRGVYTSG KGSSAVGLTA YITRDVDTKQ LVLESGALVL SDGGVCCIDE FDKMSDS TR SVLHEVMEQQ TISIAKAGII TTLNARSSIL ASANPIGSRY NPNLPVTENI DLPPPLLSRF DLVYLVLDKV DEKNDREL A KHLTNLYLED KPEHISQDDV LPVEFLTMYI SYAKEHIHPI ITEAAKTELV RAYVGMRKMG DDSRSDEKRI TATTRQLES MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP KTGKIDMNLV QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQ INEHSQDRVE SSDIQEALSR LQQEDKVIVL GEGVRRSVRL NNRV

UniProtKB: DNA replication licensing factor MCM4

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分子 #4: DNA helicase

分子名称: DNA helicase / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 86.505734 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPL FETAITQVAK RISILSRAQS ANNNDKDPEN TSMDTDSLLL NSLPTFQLIL NSNANQIPLR DLDSEHVSKI V RLSGIIIS ...文字列:
MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPL FETAITQVAK RISILSRAQS ANNNDKDPEN TSMDTDSLLL NSLPTFQLIL NSNANQIPLR DLDSEHVSKI V RLSGIIIS TSVLSSRATY LSIMCRNCRH TTSITINNFN SITGNTVSLP RSCLSTIESE SSMANESNIG DESTKKNCGP DP YIIIHES SKFIDQQFLK LQEIPELVPV GEMPRNLTMT CDRYLTNKVI PGTRVTIVGI YSIYNSKNGA GSGRSGGGNG GSG VAIRTP YIKILGIQSD VETSSIWNSV TMFTEEEEEE FLQLSRNPKL YEILTNSIAP SIFGNEDIKK AIVCLLMGGS KKIL PDGMR LRGDINVLLL GDPGTAKSQL LKFVEKVSPI AVYTSGKGSS AAGLTASVQR DPMTREFYLE GGAMVLADGG VVCID EFDK MRDEDRVAIH EAMEQQTISI AKAGITTVLN SRTSVLAAAN PIYGRYDDLK SPGDNIDFQT TILSRFDMIF IVKDDH NEE RDISIANHVI NIHTGNANAM QNQQEENGSE ISIEKMKRYI TYCRLKCAPR LSPQAAEKLS SNFVTIRKQL LINELES TE RSSIPITIRQ LEAIIRITES LAKLELSPIA QERHVDEAIR LFQASTMDAA SQDPIGGLNQ ASGTSLSEIR RFEQELKR R LPIGWSTSYQ TLRREFVDTH RFSQLALDKA LYALEKHETI QLRHQGQNIY RSGV

UniProtKB: DNA replication licensing factor MCM5

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分子 #5: DNA replication licensing factor MCM6

分子名称: DNA replication licensing factor MCM6 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 113.110211 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP FVNDSTQFSS QRLQTDGSAT NDMEGNEPA RSFKSRALNH VKKVDDVTGE KVREAFEQFL EDFSVQSTDT GEVEKVYRAQ IEFMKIYDLN TIYIDYQHLS M RENGALAM ...文字列:
MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP FVNDSTQFSS QRLQTDGSAT NDMEGNEPA RSFKSRALNH VKKVDDVTGE KVREAFEQFL EDFSVQSTDT GEVEKVYRAQ IEFMKIYDLN TIYIDYQHLS M RENGALAM AISEQYYRFL PFLQKGLRRV VRKYAPELLN TSDSLKRSEG DEGQADEDEQ QDDDMNGSSL PRDSGSSAAP GN GTSAMAT RSITTSTSPE QTERVFQISF FNLPTVHRIR DIRSEKIGSL LSISGTVTRT SEVRPELYKA SFTCDMCRAI VDN VEQSFK YTEPTFCPNP SCENRAFWTL NVTRSRFLDW QKVRIQENAN EIPTGSMPRT LDVILRGDSV ERAKPGDRCK FTGV EIVVP DVTQLGLPGV KPSSTLDTRG ISKTTEGLNS GVTGLRSLGV RDLTYKISFL ACHVISIGSN IGASSPDANS NNRET ELQM AANLQANNVY QDNERDQEVF LNSLSSDEIN ELKEMVKDEH IYDKLVRSIA PAVFGHEAVK KGILLQMLGG VHKSTV EGI KLRGDINICV VGDPSTSKSQ FLKYVVGFAP RSVYTSGKAS SAAGLTAAVV RDEEGGDYTI EAGALMLADN GICCIDE FD KMDISDQVAI HEAMEQQTIS IAKAGIHATL NARTSILAAA NPVGGRYNRK LSLRGNLNMT APIMSRFDLF FVILDDCN E KIDTELASHI VDLHMKRDEA IEPPFSAEQL RRYIKYARTF KPILTKEARS YLVEKYKELR KDDAQGFSRS SYRITVRQL ESMIRLSEAI ARANCVDEIT PSFIAEAYDL LRQSIIRVDV DDVEMDEEFD NIESQSHAAS GNNDDNDDGT GSGVITSEPP ADIEEGQSE ATARPGTSEK KKTTVTYDKY VSMMNMIVRK IAEVDREGAE ELTAVDIVDW YLLQKENDLG SLAEYWEERR L AFKVIKRL VKDRILMEIH GTRHNLRDLE NEENENNKTV YVIHPNCEVL DQLEPQDSS

UniProtKB: DNA replication licensing factor MCM6

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分子 #6: DNA replication licensing factor MCM7

分子名称: DNA replication licensing factor MCM7 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 95.049875 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG PKYMAMLQKV ANRELNSVII DLDDILQYQ NEKFLQGTQA DDLVSAIQQN ANHFTELFCR AIDNNMPLPT KEIDYKDDVL DVILNQRRLR NERMLSDRTN E IRSENLMD ...文字列:
MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG PKYMAMLQKV ANRELNSVII DLDDILQYQ NEKFLQGTQA DDLVSAIQQN ANHFTELFCR AIDNNMPLPT KEIDYKDDVL DVILNQRRLR NERMLSDRTN E IRSENLMD TTMDPPSSMN DALREVVEDE TELFPPNLTR RYFLYFKPLS QNCARRYRKK AISSKPLSVR QIKGDFLGQL IT VRGIITR VSDVKPAVEV IAYTCDQCGY EVFQEVNSRT FTPLSECTSE ECSQNQTKGQ LFMSTRASKF SAFQECKIQE LSQ QVPVGH IPRSLNIHVN GTLVRSLSPG DIVDVTGIFL PAPYTGFKAL KAGLLTETYL EAQFVRQHKK KFASFSLTSD VEER VMELI TSGDVYNRLA KSIAPEIYGN LDVKKALLLL LVGGVDKRVG DGMKIRGDIN VCLMGDPGVA KSQLLKAICK ISPRG VYTT GKGSSGVGLT AAVMKDPVTD EMILEGGALV LADNGICCID EFDKMDESDR TAIHEVMEQQ TISISKAGIN TTLNAR TSI LAAANPLYGR YNPRLSPLDN INLPAALLSR FDILFLMLDI PSRDDDEKLA EHVTYVHMHN KQPDLDFTPV EPSKMRE YI AYAKTKRPVM SEAVNDYVVQ AYIRLRQDSK REMDSKFSFG QATPRTLLGI IRLSQALAKL RLADMVDIDD VEEALRLV R VSKESLYQET NKSKEDESPT TKIFTIIKKM LQETGKNTLS YENIVKTVRL RGFTMLQLSN CIQEYSYLNV WHLINEGNT LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI DLQDA

UniProtKB: DNA replication licensing factor MCM7

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分子 #7: DNA replication complex GINS protein PSF1

分子名称: DNA replication complex GINS protein PSF1 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 24.230576 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAY QRLRTDILDS MAWNNNGLDL MSSITFSQQD TNNLSHQEQE YLKEYCDLIT DLKSGDLVDI DLSGSLVPPS D VFIDVRVL ...文字列:
MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAY QRLRTDILDS MAWNNNGLDL MSSITFSQQD TNNLSHQEQE YLKEYCDLIT DLKSGDLVDI DLSGSLVPPS D VFIDVRVL KDAGEIQTEY GVFNLIKDSQ FFVRQSDVER LIQQGYLQKI

UniProtKB: DNA replication complex GINS protein PSF1

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分子 #8: DNA replication complex GINS protein PSF2

分子名称: DNA replication complex GINS protein PSF2 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 25.096807 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL NNMVAMRSTE VVLWIALLLK QQSKCSIVA PQWLTTKELD RKIQYEKTHP DRFSELPWNW LVLARILFNK AKDDFHDPIH ELRGKIQDLR EIRQIKVLKG L KYLNESHL ...文字列:
MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL NNMVAMRSTE VVLWIALLLK QQSKCSIVA PQWLTTKELD RKIQYEKTHP DRFSELPWNW LVLARILFNK AKDDFHDPIH ELRGKIQDLR EIRQIKVLKG L KYLNESHL QLDNLSLLEI NELRPFITEI MDKLREIHTA SLTAGTENDE EEFNI

UniProtKB: DNA replication complex GINS protein PSF2

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分子 #9: DNA replication complex GINS protein PSF3

分子名称: DNA replication complex GINS protein PSF3 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 22.00416 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MGYYDIDDVL ADGTEFPCKF QYDIPGLGYL ENNPGRPITK NTKLNLPLWL ARILAIVGGD EALVDEEPVP FVELLPPDMF STKVMNAIK TDPVALDLHS INSHFFSLAI KWIMLFSEKE LANVVSELLL QRAQELNHHA SSLSIDLNAD STGKNSANTN I ATSTFLLK ...文字列:
MGYYDIDDVL ADGTEFPCKF QYDIPGLGYL ENNPGRPITK NTKLNLPLWL ARILAIVGGD EALVDEEPVP FVELLPPDMF STKVMNAIK TDPVALDLHS INSHFFSLAI KWIMLFSEKE LANVVSELLL QRAQELNHHA SSLSIDLNAD STGKNSANTN I ATSTFLLK LEEMEKEIYK KSHESYKDTK RWMFKK

UniProtKB: DNA replication complex GINS protein PSF3

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分子 #10: DNA replication complex GINS protein SLD5

分子名称: DNA replication complex GINS protein SLD5 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 33.983617 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLN RISMQSQLIE NISMGFLDMQ NASNANPPMP NESKLPLLCM ETELERLKFV IRSYIRCRLS KIDKFSLYLR Q LNEDENSL ...文字列:
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLN RISMQSQLIE NISMGFLDMQ NASNANPPMP NESKLPLLCM ETELERLKFV IRSYIRCRLS KIDKFSLYLR Q LNEDENSL ISLTDLLSKD EIKYHDTHSL IWLKLVNDSI LKYMPEELQA INDTEGSVNM IDEPDWNKFV FIHVNGPPDG KW NEDPLLQ ENEFGKPCYT VTIPDLKEEV ELTIGSIYVM RYEVIRDLLR DDKVALI

UniProtKB: DNA replication complex GINS protein SLD5

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分子 #11: Cell division control protein 45,Cell division control protein 45

分子名称: Cell division control protein 45,Cell division control protein 45
タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 75.154703 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGV IDLEAFLEID PQEYVIDTDE KSGEQSFRRD IYVLDAHRPW NLDNIFGSQI IQCFDDGTVD DTLGEQKEAY Y KLLELDEE ...文字列:
MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGV IDLEAFLEID PQEYVIDTDE KSGEQSFRRD IYVLDAHRPW NLDNIFGSQI IQCFDDGTVD DTLGEQKEAY Y KLLELDEE SGDDELSGDE NDNNGGDDEA TDADEVTDED YKDDDGDYKD DDETISNKRG NSSIGPNDLS KRKQRKKQIH EY EGVLEEY YSQGTTVVNS ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK RLTPSSRNSV KTP DTLTLN IQPDYYLFLL RHSSLYDSFY YSNYVNAKLS LWNENGKKRL HKMFARMGIP LSTAQETWLY MDHSIKRELG IIFD KNLDR YGLQDIIRDG FVRTLGYRGS ISASEFVEAL TALLEVGNST DKDSVKINND NNDDTDGEEE EDNSAQKLTN LRKRW VSNF WLSWDALDDR KVELLNRGIQ LAQDLQRAIF NTGVAILEKK LIKHLRIYRL CVLQDGPDLD LYRNPLTLLR LGNWLI ECC AESEDKQLLP MVLASIDENT DTYLVAGLTP RYPRGLDTIH TKKPILNNFS MAFQQITAET DAKVRIDNFE SSIIEIR RE DLSPFLEKLT LSGLL

UniProtKB: Cell division control protein 45, Cell division control protein 45

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分子 #12: DNA polymerase alpha-binding protein

分子名称: DNA polymerase alpha-binding protein / タイプ: protein_or_peptide / ID: 12 / コピー数: 3 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 108.610148 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEMVSVI DKLVFDFGGK TLVSLAPDNN TLCVANKNGL TKILKTNNPE EEPETLDSS KLVSSIKCYS NSHFLMTTMQ GDALRYNIDS SQEELLARFA LPLRDCCVIH SGKMAVFGGD DLELILLELD D ETHKKHAI ...文字列:
MKRRWKKNFI AVSAANRFKK ISSSGALENL YFQGEMVSVI DKLVFDFGGK TLVSLAPDNN TLCVANKNGL TKILKTNNPE EEPETLDSS KLVSSIKCYS NSHFLMTTMQ GDALRYNIDS SQEELLARFA LPLRDCCVIH SGKMAVFGGD DLELILLELD D ETHKKHAI KIDEQVSQIS YNSQMNILAV SMINGKVQIF SLTSTIPNKV HELNDYIVAN SYDDTHRDKI LSNMMDDIDK DN DNDLSET ADPDENNVAD PEFCAANRIC TRVAWHPKGL HFALPCADDT VKIFSIKGYS LQKTLSTNLS STKAHFIDLQ FDP LRGTYI AAVDLNNKLT VWNWETSEIH YTREFKRKIT NIAWKIQADS KTLDLVLGTW SGSIAIVQNL AESVVSNIPD QSVA ESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI TQEANAEDVF TQTHDGPSGL SEKRKYNFED EEDFIDDDDG AGYIS GKKP HNEHSYSRVH KTHSFPISLA NTGKFRYMPF SPAGTPFGFT DRRYLTMNEV GYVSTVKNSE QYSITVSFFD VGRFRE YHF EDLFGYDLCF LNEKGTLFGQ SKTGQIQYRP HDSIHSNWTK IIPLQAGERI TSVAATPVRV IVGTSLGYFR SFNQFGV PF AVEKTSPIVA LTAQNYRVFS VHYSQFHGLS YSLSELGTSS KRYYKRECPL PMSLPNINSD MKKDANLDYY NFNPMGIK S LFFSSYGDPC IFGSDNTLLL LSKWRSPEES KWLPILDSNM EIWKMSGGKE TTDIHVWPLA LAYDTLNCIL VKGKHIWPE FPLPLPSEME IRMPVFVKSK LLEENKAILN KKNEIGADTE AEEGEEDKEI QIPVSMAAEE EYLRSKVLSE LLTDTLENDG EMYGNENEV LAALNGAYDK ALLRLFASAC SDQNVEKALS LAHELKQDRA LTAAVKISER AELPSLVKKI NNIREARYEQ Q LK

UniProtKB: DNA polymerase alpha-binding protein

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分子 #15: E3 ubiquitin ligase complex SCF subunit

分子名称: E3 ubiquitin ligase complex SCF subunit / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 22.35727 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MVTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQK VIEWAEHHRD SNFPDEDDDD SRKSAPVDSW DREFLKVDQE MLYEIILAAN YLNIKPLLDA GCKVVAEMIR G RSPEEIRR ...文字列:
MVTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQK VIEWAEHHRD SNFPDEDDDD SRKSAPVDSW DREFLKVDQE MLYEIILAAN YLNIKPLLDA GCKVVAEMIR G RSPEEIRR TFNIVNDFTP EEEAAIRREN EWAEDR

UniProtKB: E3 ubiquitin ligase complex SCF subunit

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分子 #16: Protein DIA2

分子名称: Protein DIA2 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 85.368844 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: GAGMSSPGNS GVAIDSTVLK AIELGTRLFK SGEYLQAKRI FTNALRVCDS YSQEQIMRIR NAYQLDTARP DNKRLYHPRY IKILDNICA CYEKLNDLKS CLDVSQRLLK LEPGNIKCYI RCTRTLIKLK DWKRAYKTCS RGLQLCNNDS NHLRQQKQFI K NNMVQKQD ...文字列:
GAGMSSPGNS GVAIDSTVLK AIELGTRLFK SGEYLQAKRI FTNALRVCDS YSQEQIMRIR NAYQLDTARP DNKRLYHPRY IKILDNICA CYEKLNDLKS CLDVSQRLLK LEPGNIKCYI RCTRTLIKLK DWKRAYKTCS RGLQLCNNDS NHLRQQKQFI K NNMVQKQD GKRSYIDPLE ETKIAKKKKN NNVLESLPKK KIKGSTKKTD LVGNLPIEIL PIIFQRFTTK ELVTLSLVCN KW RDKILYH LDCFQEFNLA PINFKNFVKF MDFLQQNFTR TYRKYILSQV KVSSRITSEE LRITQLLFSK MPKCINIERL ILS MPTLTT TQIFKLMVRG GTDFFTRLLE LSLMITYRPD KQHELEILQT CPLLKKIELI FVNSLVPIFD GNNSVGRDGS FNVM ARHTN MQISTADNDE QGIVEEKVIY SELEKITLIC DKKKIKNFPL CRALLRGQFP LLQKLTITGV TFPMNNQDIM NFQWL LNFP DLKELWIEDN DNCELSKFLQ LLKFSNVWKN LEKLTFRENK LYPIVNLDED QPVTNDDEVP SMLFYKENLQ NLEKLD LMG TSISGSALTR LCEQEYLDGR KLRSLNIGNC PNIQFPNNHA HTARMILDVN AVLKRLSKLE EINLSHLSSL NDSTMKS FI INVPFLENLK RLDISHNFEI TGISIYEFLK KFQMDHDNEA GGQPLAYLNI DGCSQVSHIT VNMIRAQNLV TQVDCVYE R DVWRKFGINS YSYS

UniProtKB: Protein DIA2

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分子 #17: DNA polymerase epsilon catalytic subunit A

分子名称: DNA polymerase epsilon catalytic subunit A / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA-directed DNA polymerase
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 255.890422 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY NGRFDAKDID QIPGRVGWLT NMHATLVSQ ETLSSGSNGG GNSNDGERVT TNQGISGVDF YFLDEEGGSF KSTVVYDPYF FIACNDESRV NDVEELVKKY L ESCLKSLQ ...文字列:
MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY NGRFDAKDID QIPGRVGWLT NMHATLVSQ ETLSSGSNGG GNSNDGERVT TNQGISGVDF YFLDEEGGSF KSTVVYDPYF FIACNDESRV NDVEELVKKY L ESCLKSLQ IIRKEDLTMD NHLLGLQKTL IKLSFVNSNQ LFEARKLLRP ILQDNANNNV QRNIYNVAAN GSEKVDAKHL IE DIREYDV PYHVRVSIDK DIRVGKWYKV TQQGFIEDTR KIAFADPVVM AFAIATTKPP LKFPDSAVDQ IMMISYMIDG EGF LITNRE IISEDIEDFE YTPKPEYPGF FTIFNENDEV ALLQRFFEHI RDVRPTVIST FNGDFFDWPF IHNRSKIHGL DMFD EIGFA PDAEGEYKSS YCSHMDCFRW VKRDSYLPQG SQGLKAVTQS KLGYNPIELD PELMTPYAFE KPQHLSEYSV SDAVA TYYL YMKYVHPFIF SLCTIIPLNP DETLRKGTGT LCEMLLMVQA YQHNILLPNK HTDPIERFYD GHLLESETYV GGHVES LEA GVFRSDLKNE FKIDPSAIDE LLQELPEALK FSVEVENKSS VDKVTNFEEI KNQITQKLLE LKENNIRNEL PLIYHVD VA SMYPNIMTTN RLQPDSIKAE RDCASCDFNR PGKTCARKLK WAWRGEFFPS KMDEYNMIKR ALQNETFPNK NKFSKKKV L TFDELSYADQ VIHIKKRLTE YSRKVYHRVK VSEIVEREAI VCQRENPFYV DTVKSFRDRR YEFKGLAKTW KGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPE TYFFTLENGK KLYLSYPCSM LNYRVHQKFT NHQYQELKDP LNYIYETHSE NTIFFEVDGP YKAMILPSSK E EGKGIKKR YAVFNEDGSL AELKGFELKR RGELQLIKNF QSDIFKVFLE GDTLEGCYSA VASVCNRWLD VLDSHGLMLE DE DLVSLIC ENRSMSKTLK EYEGQKSTSI TTARRLGDFL GEDMVKDKGL QCKYIISSKP FNAPVTERAI PVAIFSADIP IKR SFLRRW TLDPSLEDLD IRTIIDWGYY RERLGSAIQK IITIPAALQG VSNPVPRVEH PDWLKRKIAT KEDKFKQTSL TKFF SKTKN VPTMGKIKDI EDLFEPTVEE DNAKIKIART TKKKAVSKRK RNQLTNEEDP LVLPSEIPSM DEDYVGWLNY QKIKW KIQA RDRKRRDQLF GNTNSSRERS ALGSMIRKQA ESYANSTWEV LQYKDSGEPG VLEVFVTING KVQNITFHIP KTIYMK FKS QTMPLQKIKN CLIEKSSASL PNNPKTSNPA GGQLFKITLP ESVFLEEKEN CTSIFNDENV LGVFEGTITP HQRAIMD LG ASVTFRSKAM GALGKGIQQG FEMKDLSMAE NERYLSGFSM DIGYLLHFPT SIGYEFFSLF KSWGDTITIL VLKPSNQA Q EINASSLGQI YKQMFEKKKG KIETYSYLVD IKEDINFEFV YFTDISKLYR RLSQETTKLK EERGLQFLLL LQSPFITKL LGTIRLLNQM PIVKLSLNEV LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL KKENIVLWW NEKAPLPDHG GIQNDFDLNT SWIMNDSEFP KINNSGVYDN VVLDVGVDNL TVNTILTSAL INDAEGSDLV N NNMGIDDK DAVINSPSEF VHDAFSNDAL NVLRGMLKEW WDEALKENST ADLLVNSLAS WVQNPNAKLF DGLLRYHVHN LT KKALLQL VNEFSALGST IVYADRNQIL IKTNKYSPEN CYAYSQYMMK AVRTNPMFSY LDLNIKRYWD LLIWMDKFNF SGL ACIEIE EKENQDYTAV SQWQLKKFLS PIYQPEFEDW MMIILDSMLK TKQSYLKLNS GTQRPTQIVN VKKQDKEDSV ENSL NGFSH LFSKPLMKRV KKLFKNQQEF ILDPQYEADY VIPVLPGSHL NVKNPLLELV KSLCHVMLLS KSTILEIRTL RKELL KIFE LREFAKVAEF KDPSLSLVVP DFLCEYCFFI SDIDFCKAAP ESIFSCVRCH KAFNQVLLQE HLIQKLRSDI ESYLIQ DLR CSRCHKVKRD YMSAHCPCAG AWEGTLPRES IVQKLNVFKQ VAKYYGFDIL LSCIADLTI

UniProtKB: DNA polymerase epsilon catalytic subunit A

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分子 #18: DNA polymerase epsilon subunit B

分子名称: DNA polymerase epsilon subunit B / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 78.425852 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG ANWRQGPATI KFLEQFAAVW KQQERGLFID QSGVKEVIQ EMKEREKVEW SHEHPIQHEE NILGRTDDDE NNSDDEMPIA ADSSLQNVSL SSPMRQPTER DEYKQPFKPE S SKALDWRD ...文字列:
MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG ANWRQGPATI KFLEQFAAVW KQQERGLFID QSGVKEVIQ EMKEREKVEW SHEHPIQHEE NILGRTDDDE NNSDDEMPIA ADSSLQNVSL SSPMRQPTER DEYKQPFKPE S SKALDWRD YFKVINASQQ QRFSYNPHKM QFIFVPNKKQ NGLGGIAGFL PDIEDKVQMF LTRYYLTNDR VMRNENFQNS DM FNPLSSM VSLQNELSNT NRQQQSSSMS ITPIKNLLGR DAQNFLLLGL LNKNFKGNWS LEDPSGSVEI DISQTIPTQG HYY VPGCMV LVEGIYYSVG NKFHVTSMTL PPGERREITL ETIGNLDLLG IHGISNNNFI ARLDKDLKIR LHLLEKELTD HKFV ILGAN LFLDDLKIMT ALSKILQKLN DDPPTLLIWQ GSFTSVPVFA SMSSRNISSS TQFKNNFDAL ATLLSRFDNL TENTT MIFI PGPNDLWGSM VSLGASGTLP QDPIPSAFTK KINKVCKNVV WSSNPTRIAY LSQEIVIFRD DLSGRFKRHR LEFPFN ESE DVYTENDNMM SKDTDIVPID ELVKEPDQLP QKVQETRKLV KTILDQGHLS PFLDSLRPIS WDLDHTLTLC PIPSTMV LC DTTSAQFDLT YNGCKVINPG SFIHNRRARY MEYVPSSKKT IQEEIYI

UniProtKB: DNA polymerase epsilon subunit B

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分子 #19: Topoisomerase 1-associated factor 1

分子名称: Topoisomerase 1-associated factor 1 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 141.296875 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLL PILIDWENKC SLAAKLAKNN PDHEEFRNKA YYDKIALNCL QLLVLMTWPL IVTEQSSSNQ ITLYGELKKH Q LVYKKTIL ...文字列:
MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLL PILIDWENKC SLAAKLAKNN PDHEEFRNKA YYDKIALNCL QLLVLMTWPL IVTEQSSSNQ ITLYGELKKH Q LVYKKTIL SMESGKVLRA AIRLALDVIK IDRLSRTPRD NMVLKLVLNF FRNVIAIEPG EFTINTKKSM PKKGITSIDT LP PNVSMDD ISLNTVISSF HKNKVFGFLL TLTSSLSKEF DQDFINIPLL EIMFYFTKDV NQELLFPRQF ETGTHSKVVN KNE SSSANN IVTSAGFELS KLLQKEHQMR KNVIKHTSAR HSRFGGLLSI QTPDKTRLTV SGSQALVDEK IALQKLDDSK KWNK RIIKK HQSVAAEGLP NSLLNSQTGK AIFFTESNGK HFKEFINNFI DSGFNILLHS VTNYFTTEQD RMVTLEQVEY LLFFA WFVK YQLLRSKIDN SADIKQVSEA LKEVTFILVS SLLRSAYDLK NWTVTHAGMI AFNELLNLVS RTKAAQEEDS TDIEFI VSR LFSDERIQLL SNLPKIGSKY SLQFMKSCIE LTHSVLKVLE QYSDDKTLVI EGKSRRQKKF NISEGDITKL IEEENVD RD EALDILTSSL RSIEVNFQKV QANYMTEPVI ETYINFLERF RELEDDSIKK VFSFFHRVFV QAKEQALLFR FDLIILLR E MLSPDGLDRM SRSRKYVSQF SDYFLARLKK RLKKSPAWFV GLLFPPLHNS EVGFYQRYGE YNVLNNESMY AAPASQFKP IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRAL LLLIGYSIPR KINEPCFLPG TVEVSDLTVS CELVKKYLST PFETPNGLPS SSYLLRVRSE KDSFSHNEQD G WEGDDDYD YNDPYIVPDD QILSKSDAAY FKDLDNNASD KLKGTKFSKG IARSKKKDKR KRRKGEAKTN LPMFGDQDDE RP QTVRERH GVFSKEFISD SEDDEDLMNP IFFENETYMR WLLDKNNGQL TEDRYIQFAK FAAERMNNGG VVTGDYTSLF GGS IPSIES IRATESSSFA PDKSLISLAS HVASEMSIFD VNNNNNNQLS DDDVNSESRN SLGSSQPSNS QNMFQSEVYS RKES TKRSL EASAADESDE DEEAIRLFGK KSRVVLSQGD SDD

UniProtKB: Topoisomerase 1-associated factor 1

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分子 #20: Chromosome segregation in meiosis protein 3

分子名称: Chromosome segregation in meiosis protein 3 / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 36.588758 KDa
組換発現生物種: Saccharomyces cerevisiae (パン酵母)
配列文字列: GEMDQDFDSL LLGFNDSDSV QKDPTVPNGL DGSVVDPTIA DPTAITARKR RPQVKLTAEK LLSDKGLPYV LKNAHKRIRI SSKKNSYDN LSNIIQFYQL WAHELFPKAK FKDFMKICQT VGKTDPVLRE YRVSLFRDEM GMSFDVGTRE TGQDLERQSP M VEEHVTSA ...文字列:
GEMDQDFDSL LLGFNDSDSV QKDPTVPNGL DGSVVDPTIA DPTAITARKR RPQVKLTAEK LLSDKGLPYV LKNAHKRIRI SSKKNSYDN LSNIIQFYQL WAHELFPKAK FKDFMKICQT VGKTDPVLRE YRVSLFRDEM GMSFDVGTRE TGQDLERQSP M VEEHVTSA EERPIVADSF AQDKRNVNNV DYDNDEDDDI YHLSYRNRRG RVLDERGNNE TVLNNVVPPK EDLDALLKTF RV QGPVGLE ENEKKLLLGW LDAHRKMEKG SMTEEDVQLI QSLEEWEMND IEGQHTHYDL LPGGDEFGVD QDELDAMKEM GF

UniProtKB: Chromosome segregation in meiosis protein 3

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分子 #13: Leading strand template DNA

分子名称: Leading strand template DNA / タイプ: dna / ID: 13 / コピー数: 1 / 分類: DNA
由来(天然)生物種: Synthetic construct (人工物)
分子量理論値: 37.688645 KDa
配列文字列: (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG) (DG)(DG)(DG)(DG)(DG) ...文字列:
(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG) (DT)(DT)(DT)(DT)(DT) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)

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分子 #14: Lagging strand template DNA

分子名称: Lagging strand template DNA / タイプ: dna / ID: 14 / コピー数: 1 / 分類: DNA
由来(天然)生物種: Synthetic construct (人工物)
分子量理論値: 35.259238 KDa
配列文字列: (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DA)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC) (DC)(DC)(DC)(DC)(DC) ...文字列:
(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DA)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC) (DC)(DC)

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分子 #21: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

分子名称: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / タイプ: ligand / ID: 21 / コピー数: 3 / 式: ANP
分子量理論値: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM

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分子 #22: MAGNESIUM ION

分子名称: MAGNESIUM ION / タイプ: ligand / ID: 22 / コピー数: 3 / 式: MG
分子量理論値: 24.305 Da

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分子 #23: ZINC ION

分子名称: ZINC ION / タイプ: ligand / ID: 23 / コピー数: 7 / 式: ZN
分子量理論値: 65.409 Da

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

緩衝液pH: 7.6
グリッドモデル: Quantifoil R2/2 / 材質: COPPER / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 5 sec. / 詳細: 15 mA
凍結凍結剤: ETHANE / 詳細: Manual plunger.

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
特殊光学系エネルギーフィルター - 名称: GIF Bioquantum / エネルギーフィルター - スリット幅: 20 eV
撮影フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k)
実像数: 12730 / 平均露光時間: 4.0 sec. / 平均電子線量: 38.8 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2.2 µm / 最小 デフォーカス（公称値）: 0.4 µm / 倍率（公称値）: 81000
試料ステージ試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
ホルダー冷却材: NITROGEN
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

粒子像選択選択した数: 2160000
初期モデルモデルのタイプ: OTHER
最終 再構成解像度のタイプ: BY AUTHOR / 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3)
詳細: Composite map produced from individual cryo-EM density maps (resolutions 3.2 - 4.0 A) using Phenix combine_focused_maps. See publication for details.
使用した粒子像数: 369254
初期 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 3)
最終 角度割当タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: RELION (ver. 3)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る