- PDB-4ro0: Crystal structure of MthK gating ring in a ligand-free form -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4ro0
Title
Crystal structure of MthK gating ring in a ligand-free form
Components
Calcium-gated potassium channel MthK
Keywords
TRANSPORT PROTEIN / Rossmann fold / octamer / gating ring of a Calcium-gated potassium channel
Function / homology
Function and homology information
monoatomic cation transmembrane transporter activity / potassium ion transport / identical protein binding / metal ion binding / plasma membrane Similarity search - Function
Mass: 25447.984 Da / Num. of mol.: 40 / Fragment: MthK RCK, UNP residues 107-336 / Source method: isolated from a natural source Source: (natural) Methanothermobacter thermautotrophicus (archaea) Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H References: UniProt: O27564
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.74 Å3/Da / Density % sol: 67.07 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.4 M KCl, 0.1 M Tris and 20-25% PEGMME 350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K