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- EMDB-13434: 70S ribosome with A/P- and P/E-site tRNAs in spectinomycin-treate... -

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Basic information

Entry
Database: EMDB / ID: EMD-13434
Title70S ribosome with A/P- and P/E-site tRNAs in spectinomycin-treated Mycoplasma pneumoniae cells
Map data
Sample
  • Cell: Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneumoniae cells
    • Protein or peptide: x 49 types
    • RNA: x 4 types
Function / homology
Function and homology information


large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation ...large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / response to antibiotic / mRNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / Ribosomal protein S14, type Z / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 ...Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / Ribosomal protein S14, type Z / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L10-like domain superfamily / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L11, N-terminal / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal protein S14/S29 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature.
Similarity search - Domain/homology
Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL13 ...Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein uL11 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL33A / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesMycoplasma pneumoniae M129 (bacteria) / Mycoplasma pneumoniae (strain ATCC 29342 / M129) (bacteria)
Methodsubtomogram averaging / cryo EM / Resolution: 13.6 Å
AuthorsXue L / Lenz S / Rappsilber J / Mahamid J
Funding support United Kingdom, 4 items
OrganizationGrant numberCountry
European Research Council (ERC)760067 United Kingdom
German Research Foundation (DFG)426290502 United Kingdom
Wellcome Trust103139 United Kingdom
Wellcome Trust203149 United Kingdom
Citation
Journal: Nature / Year: 2022
Title: Visualizing translation dynamics at atomic detail inside a bacterial cell.
Authors: Liang Xue / Swantje Lenz / Maria Zimmermann-Kogadeeva / Dimitry Tegunov / Patrick Cramer / Peer Bork / Juri Rappsilber / Julia Mahamid /
Abstract: Translation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram analysis to ...Translation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram analysis to visualize the structural dynamics of translation inside the bacterium Mycoplasma pneumoniae. To interpret the functional states in detail, we first obtain a high-resolution in-cell average map of all translating ribosomes and build an atomic model for the M. pneumoniae ribosome that reveals distinct extensions of ribosomal proteins. Classification then resolves 13 ribosome states that differ in their conformation and composition. These recapitulate major states that were previously resolved in vitro, and reflect intermediates during active translation. On the basis of these states, we animate translation elongation inside native cells and show how antibiotics reshape the cellular translation landscapes. During translation elongation, ribosomes often assemble in defined three-dimensional arrangements to form polysomes. By mapping the intracellular organization of translating ribosomes, we show that their association into polysomes involves a local coordination mechanism that is mediated by the ribosomal protein L9. We propose that an extended conformation of L9 within polysomes mitigates collisions to facilitate translation fidelity. Our work thus demonstrates the feasibility of visualizing molecular processes at atomic detail inside cells.
#1: Journal: Biorxiv / Year: 2021
Title: Visualizing translation dynamics at atomic detail inside a bacterial cell
Authors: Xue L / Lenz S / Zimmermann-Kogadeeva M / Tegunov D / Cramer P / Bork P / Rappsilber J / Mahamid J
History
DepositionAug 19, 2021-
Header (metadata) releaseMay 25, 2022-
Map releaseMay 25, 2022-
UpdateOct 19, 2022-
Current statusOct 19, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13434.png

Downloads & links

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Map

FileDownload / File: emd_13434.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.7005 Å
Density
Contour LevelBy AUTHOR: 0.22
Minimum - Maximum-0.2778617 - 0.74296427
Average (Standard dev.)0.0154275345 (±0.06939922)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 435.328 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_13434_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_13434_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_13434_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneum...

EntireName: Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneumoniae cells
Components
  • Cell: Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneumoniae cells
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L10
    • Protein or peptide: 50S ribosomal protein L11
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 1Ribosome
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 16S ribosomal RNA
    • RNA: tRNA-Phe

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Supramolecule #1: Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneum...

SupramoleculeName: Cryo-electron tomograms of spectinomycin-treated Mycoplasma pneumoniae cells
type: cell / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Ribosome sub-tomograms extracted in silico from tomograms of intact cell, refinement with a 70S mask
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)

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Macromolecule #1: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 5.616773 KDa
SequenceString:
MKRTYQPSKL KRAKTHGFLA RMATASGRKV LKLRRKKQRA QLTVSSER

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Macromolecule #2: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 6.793135 KDa
SequenceString:
MKVKSAAKKR FKLTKSGQIK RKHAYTSHLA PHKTTKQKRH LRKQGTVSAS DFKRIGNLI

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Macromolecule #3: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 4.400488 KDa
SequenceString:
MKVRASVKPI CKDCKIIKRH QIVRVICKTQ KHKQRQG

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Macromolecule #4: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 33.468629 KDa
SequenceString: MSELITTPVE TTAKAELVSL AKLGEMRTHV GMVKRYWNPK MGFFIEPERK HNNDHFVLEL QRQSLQTAYN YVKEVAQNNG QILFVGTKN DYVKKLVNNI AKRVDVAFIT QRWLGGTLTN FKTLSISINK LNKLVEKQAE NAADLTKKEN LMLSREIERL E KFFGGVKS ...String:
MSELITTPVE TTAKAELVSL AKLGEMRTHV GMVKRYWNPK MGFFIEPERK HNNDHFVLEL QRQSLQTAYN YVKEVAQNNG QILFVGTKN DYVKKLVNNI AKRVDVAFIT QRWLGGTLTN FKTLSISINK LNKLVEKQAE NAADLTKKEN LMLSREIERL E KFFGGVKS LKRLPNLLIV DDPVYEKNAV AEANILRIPV VALCNTNTNP ELVDFIIPAN NHQPQSTCLL MNLLADAVAE AK AMPTMFA YKPDEEIQIE IPQKQEAPRQ VVNRANSKQI TSQRLNITRN PEVLTRE

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Macromolecule #5: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 30.657205 KDa
SequenceString: MGQKVNSNGL RFGINKNWIS RWTANSHAQT AKWLIEDEKI RNLFFVNYRN AQVSNVEIER TQATVDVFVY AAQPAFLIGS ENKNIQKIT KQIKQIIGRT TNLDLTINEI GSPMLSARII ARDLANAIEA RVPLRTAMRQ SLIKVLKAGA NGIKVLVSGR L NGAEIARD ...String:
MGQKVNSNGL RFGINKNWIS RWTANSHAQT AKWLIEDEKI RNLFFVNYRN AQVSNVEIER TQATVDVFVY AAQPAFLIGS ENKNIQKIT KQIKQIIGRT TNLDLTINEI GSPMLSARII ARDLANAIEA RVPLRTAMRQ SLIKVLKAGA NGIKVLVSGR L NGAEIARD KMYIEGNMPL STLRADIDYA LEKAQTTYGV IGVKVWINRG MIYTKGLNRT PAHILHPQKK QPNRQNQQPR HF NQGQVLS ANKLTGSDVE TSSIQALTKP NKEDKQ

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Macromolecule #6: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 23.817645 KDa
SequenceString: MKYTGSIFKR SRRLGFSLLE NNKEFSKGKK RKTIPGQHGN RFRSSTMSGY AQQLQEKQRM QYMYGITDKQ FRRLFRLVLK QRGNLAVNL FRVLESRLDN IVYRMGFAPT RRSARQLVNH GHVLLNDRTV DTPSIILNPG DKVRLKAKTI KIPIVKAASE S GVVSPFVE ...String:
MKYTGSIFKR SRRLGFSLLE NNKEFSKGKK RKTIPGQHGN RFRSSTMSGY AQQLQEKQRM QYMYGITDKQ FRRLFRLVLK QRGNLAVNL FRVLESRLDN IVYRMGFAPT RRSARQLVNH GHVLLNDRTV DTPSIILNPG DKVRLKAKTI KIPIVKAASE S GVVSPFVE TNNKTFEGTY VRFPERSELP AGINESYVVE WYKRLVK

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Macromolecule #7: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 24.138008 KDa
SequenceString: MTDQNQKANQ GNGLQTTNLQ AHAQRKHNLR PSSEGIKKAV SKKEGGGHNR NNQNRRFQKP AFKSEFEERI VKLKRISKTT KGGRNMRFS VLVVVGNRKG KIGYGIAKAL EVPNAIKKAI KAAHNSLHTI EIHKGSIYHE VIGRSGASRV LLKPAPQGTG I IAGGAIRA ...String:
MTDQNQKANQ GNGLQTTNLQ AHAQRKHNLR PSSEGIKKAV SKKEGGGHNR NNQNRRFQKP AFKSEFEERI VKLKRISKTT KGGRNMRFS VLVVVGNRKG KIGYGIAKAL EVPNAIKKAI KAAHNSLHTI EIHKGSIYHE VIGRSGASRV LLKPAPQGTG I IAGGAIRA IIELAGYSDI YTKNLGRNTP INMIHATMDG ILKQLSPRRV AILRNKNLNE L

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Macromolecule #8: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 25.430465 KDa
SequenceString: MQYNIILLVD GSLSLEQANQ VNEKQQQTLT NVEGLQTEYL GLKELAYPIK KQLSAHYYRW KFSGDNQSTK DFKRTANINK QVLRELIIN LEREYGYLAS INPKKQQLAL QKRAKYDEII ARENNPENPD VPVTSGLAST QPRLSRTEKA QKPKEELWDV V QKMGNFDS ...String:
MQYNIILLVD GSLSLEQANQ VNEKQQQTLT NVEGLQTEYL GLKELAYPIK KQLSAHYYRW KFSGDNQSTK DFKRTANINK QVLRELIIN LEREYGYLAS INPKKQQLAL QKRAKYDEII ARENNPENPD VPVTSGLAST QPRLSRTEKA QKPKEELWDV V QKMGNFDS VQANPYRPRF KRFNAEHVNQ RQNQQNNNNN RFDRNRNRQH NRFKDKQ

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Macromolecule #9: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 17.897029 KDa
SequenceString:
MRKNRAPKRT VLPDPVFNNT LVTRIINVIM EDGKKGLAQR ILYGAFDLIE QRTKEKPLTV FERAVGNVMP RLELRVRRIA GSNYQVPTE VPQDRKIALA LRWIAMFARK RHEKTMLEKI ANEIIDASNN TGAAIKKKDD THKMAEANKA FAHMRW

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Macromolecule #10: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 15.903083 KDa
SequenceString:
MITTTKPIKA HFDPVADLLT KINNARKAKL MTVTTIASKL KIAILEILVK EGYLANFQVL ENKSKTKRIV TFNLKYTQRR IPSINGVKQ ISKPGLRIYR PFEKLPLVLN GLGIAIISTS DGVMTDKVAR LKKIGGEILA YVW

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Macromolecule #11: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 15.149735 KDa
SequenceString:
MEKQSYYGLG RRKSSSAKVY LTPTQDKGKI TVNRRDPSEY FPNKLVIQDM EQPLDLTDLK KNFDINVVVK GGGFTGQAGA IRLGIVRAL LQFNPELKKI LKSKKLTTRD KRVKERKKFG LYGARRAPQF TKR

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Macromolecule #12: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 12.226571 KDa
SequenceString:
MNAANAVKYP ELKIKLESYD STLLDLTTKK IVEVVKGVDV KIKGPLPLPT KKEVITIIRS PHVDKASREQ FEKNRHKRLM ILVDVNQGA IDSLKRIKIP VGVTLRFSK

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Macromolecule #13: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 12.709851 KDa
SequenceString:
MAKKKKINVS SGIIHVSCSP NNTIVSASDP GGNVLCWASS GTMGFKGSRK KTPYSAGIAA DKVAKTVKEM GMATVKLFVK GTGRGKDTA IRSFANAGLS ITEINEKTPI PHNGCKPPKR PR

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Macromolecule #14: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 15.66658 KDa
SequenceString:
MATIAQLIRK PRKKKKVKSK SPALHYNLNL LNKKVTNVYS PLKRGVCTRV GTMTPKKPNS ALRKYAKVRL TNGFEVLTYI PGEGHNLQE HSVTLLRGGR VKDLPGVRYH IVRGTLDTVG VEKRRQQRSA YGAKKPKAKS

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Macromolecule #15: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 14.209607 KDa
SequenceString:
MARILGIDIP NQKRIEIALT YIFGIGLSRS QAILKQANIN PDKRVKDLTE EEFVAIRNVA SAYKIEGDLR REIALNIKHL SEIGAWRGL RHRKNLPVRG QRTRTNARTR KGPRKTVANK KIESK

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Macromolecule #16: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 6.901364 KDa
SequenceString:
MAKKSLKVKQ TRIPKFAVRA YTRCQRCGRA RAVLSHFGVC RLCFRELAYA GAIPGVKKAS W

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Macromolecule #17: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 9.921687 KDa
SequenceString:
MQIDKNGIIK SAQLHDKDVG SIQVQVSLLT SQIKQLTDHL LANKKDFISK RGLYAKVSKR KRLLKYLKHN DLEAYRNLVK TLNLRG

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Macromolecule #18: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)
Molecular weightTheoretical: 10.806921 KDa
SequenceString:
MVKIRLMRMG RVHYPTYRIV AVDSRVKRDG KYIALIGHLN PALKENKCKI DEAVALEWLN KGAKPTDTVR SLFSQTGLWK KFVESKKKP VAKSK

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Macromolecule #19: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 9.859643 KDa
SequenceString:
MKRNQRKVLI GIVKSTKNAK TATVQVESRF KHPLYHKSVV RHKKYQAHNE GEVLAKDGDK VQIVETRPLS ATKRFRIAKI IERAK

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Macromolecule #20: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 12.411522 KDa
SequenceString:
MMNNEHDNFQ KEVETTTETT FNREEGKRMV RPLFKRSKKY CRFCAIGQLR IDLIDDLEAL KRFLSPYAKI NPRRITGNCQ MHQRHVAKA LKRARYLALV PFVKD

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Macromolecule #21: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 10.057626 KDa
SequenceString:
MSRSAKKGAF VDAHLLKKVI DMNKQEKKRP IKTWSRRSTI FPEFVGNTFA VHNGKTFINV YVTDDMVGHK LGEFSPTRNF KQHTANR

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Macromolecule #22: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 9.993599 KDa
SequenceString:
MANIKSNEKR LRQNIKRNLN NKGQKTKLKT NVKNFHKEIN LDNLGNVYSQ ADRLARKGII STNRARRLKS RNVAVLNKTQ VTAVEGK

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Macromolecule #23: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 7.539182 KDa
SequenceString:
MPKIEVKNDD LELALKKFKR VSLEIRRLAQ RHEYHLRKGM RLREKRKIAQ KKRRKFRNMV

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Macromolecule #24: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 31.966871 KDa
SequenceString: MPIKKIISRS NSGIHHSTVI DYKKLLTTNK NKPEKSLLVT LKKHGGRNNQ GKITVRHQGG RNKRKYRIID FKRTHYDNIE ATVKSIEYD PNRSCFVSLI TYANGAKSYI ISPDGIKVGD KILASEHPID IKPGFSMPLA FIPEGTQVHN IELHPKGGGQ I ARSAGSYA ...String:
MPIKKIISRS NSGIHHSTVI DYKKLLTTNK NKPEKSLLVT LKKHGGRNNQ GKITVRHQGG RNKRKYRIID FKRTHYDNIE ATVKSIEYD PNRSCFVSLI TYANGAKSYI ISPDGIKVGD KILASEHPID IKPGFSMPLA FIPEGTQVHN IELHPKGGGQ I ARSAGSYA RILGQDETGK YVILQLLSGE TRKFLKECRA TVGVVSNLDH NLVVIGKAGR NRHRGIRPTV RGSAMNPNDH PH GGGEGRS PVGRDAPRTP WGKRHMGVKT RNMKKASTNL IIRNRKGEQY

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Macromolecule #25: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 31.298172 KDa
SequenceString: MEIRGIFGVK VGMSQVFTTN NERLPITVIY CEPNQVAGVK TEAKDKYSAT LLSFDTVENK KLNKPQQGFF EKNNLKPTKH LQEIRNMTG FEMGQQITPQ NLFQVGEYVD VSAISKGRGF TGAIKRWNFK IGPLGHGAGY PHRFQGSVQA GRGGASAQRV F KGKKMSGH ...String:
MEIRGIFGVK VGMSQVFTTN NERLPITVIY CEPNQVAGVK TEAKDKYSAT LLSFDTVENK KLNKPQQGFF EKNNLKPTKH LQEIRNMTG FEMGQQITPQ NLFQVGEYVD VSAISKGRGF TGAIKRWNFK IGPLGHGAGY PHRFQGSVQA GRGGASAQRV F KGKKMSGH YGHEKVTVQN LRIVGFDEAN MLVLVSGAIA GPEGGVVLIR TAKKKPGVVK PIELAVQTEK APEAKPAKLS KK KQAKELA KAQAANQQTV EAKVDTPVVE PKPTEVKKAA PVVEKKGEDK

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Macromolecule #26: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 23.632572 KDa
SequenceString: MAKLKLIKID GSFETEPVKL SPGLIAKELK QQPVFDAVLV EQASWRQGTH SILTKGEVRG GGKKPYKQKH TGKARQGSTR NPHFVGGGI VFGPKPNRNY SLKLNKKAHT AALHTVWSEK LASDNTHLVD QNLFNKTEGK TKVMMQFLKS AKLLDKNVLF V VNTLNTNL ...String:
MAKLKLIKID GSFETEPVKL SPGLIAKELK QQPVFDAVLV EQASWRQGTH SILTKGEVRG GGKKPYKQKH TGKARQGSTR NPHFVGGGI VFGPKPNRNY SLKLNKKAHT AALHTVWSEK LASDNTHLVD QNLFNKTEGK TKVMMQFLKS AKLLDKNVLF V VNTLNTNL EQSTSNIKNV QVKHLDKVSV RDLMLANALL VEKEVLKALE GKFK

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Macromolecule #27: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 20.270834 KDa
SequenceString:
MNNLKAHYQK TIAKELQKSF AFSSIMQVPR LEKIVINMGV GDAIRDSKFL ESALNELHLI SGQKPVATKA KNAISTYKLR AGQLIGCKV TLRGERMWAF LEKLIYVALP RVRDFRGLSL KSFDGRGNYT IGIKEQIIFP EIVYDDIKRI RGFDVTLVTS T NKDSEALA LLRALNLPLV KG

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Macromolecule #28: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 20.624062 KDa
SequenceString:
MSKIGNRTIT LDPAKVNLNF QKDHIAVKGP LGQIELKLPP NLPLKFELKD NNLQITRNNE LKQSKIFHGT YNALITNAII GVTQGFEKK LRLVGVGYRA NVEGETLNLQ LGYSHPIKEK IPKGLTVKVE KNTEITISGI SKELVGQFAT EVRKWRKPEP Y KGKGVLYF DEVIVRKAGK TAEGKK

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Macromolecule #29: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae (strain ATCC 29342 / M129) (bacteria)
Strain: ATCC 29342 / M129
Molecular weightTheoretical: 17.185037 KDa
SequenceString:
MKVILKQDVS NLGKRFDVVD VKDGYAIHFL FPKKLAAPLT KKSLQDRDLF LKKQQEHYEI NKALSHKLKE VIEQTELHFS LKEHNGRPY GSIITKQIIN QAHTKGMALQ KFMFKDNVRL GFGDHEITLH IFEDTTAVLK VKVTPDNGVK

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Macromolecule #30: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 17.645719 KDa
SequenceString:
MEAKKDKAQQ VADVSHLLST SAGFVIFDYT SMSAIEATSI RKKLFKNGSK IKVIKNNILR RALKAGKFEG IDETAIKGKL AVAVGVNEI VETLKAVDGV VKAKEAMNFV CGYFDNRAFN SADLEKIAKL PGRNELYGMF LSVLQAPLRK FLYALEAVKA A K

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Macromolecule #31: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 14.80361 KDa
SequenceString:
MAKKTITRIA KINLLGGQAK PGPALASVGI NMGEFTKQFN EKTKDKQGEM IPCVITAYND KSFDFILKTT PVSILLKQAA KLEKGAKNA KTIVGKITMA KAKEIAQYKL VDLNANTVEA ALKMVLGTAK QMGIEVIE

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Macromolecule #32: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 16.821477 KDa
SequenceString:
MQKTSMLTKE QANKRRQWYI VDAAGLVLGK LAVKAADLIR GKNKVDFTPN QDCGDYLIII NSDQVVLTGN KKENEFWYHH SQYIGGIKK VSGRDMLKKQ SDKLVYNAVK GMLPDNRLSR RWITKVHVFK GDKHNMEAQK PTTLNWS

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Macromolecule #33: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 13.478906 KDa
SequenceString:
MVSFMTRLNV ADNTGAKQVG IIKVLGSTRK RYAFLGDVVV VSVKDAIPSG MVKKGQVLRA VIVRTKKGQQ RKDGTHLKFD DNACVLIKE DKSPRGTRIF GPVARELRER GYNKILSLAV EVV

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Macromolecule #34: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 16.768812 KDa
SequenceString:
MELNQLKSVP KARNHKTKTL GRGHGSGLGK TSGRGQKGQK ARKSGLTRPG FEGGQTPLYR RLPKFGNARK GFLKQEWVVL NLNKIAKLK LDKINRASLI EKQVISAKSQ LPIKLIGHTK LEKPLHFEVH KVSKQALKAV ENANGSVKLL EK

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Macromolecule #35: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 15.646479 KDa
SequenceString:
MLQPKRTKYR KPHNVSYEGK AKGNSYVAFG EYGLVATKGN WIDARAIESA RIAISKCLGK TGKMWIRIFP HMSKTKKPLE VRMGSGKGN PEFWVAVVKQ GTVMFEVANI PESQMIKALT RAGHKLPVTW KILKREEVSA

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Macromolecule #36: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 14.270727 KDa
SequenceString:
MSYINKPGKT SAWRVMTVRQ QVSAVLAYGK IETTLKKAKN TQKRLDKLIT LAKVDNFNNR RQVKKWLLNT NLFDVDQLMD HLFSKVAPK YEKTPGGYSR VLKLGPRRGD ATEMAILQLT DAKYK

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Macromolecule #37: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 13.073256 KDa
SequenceString:
MKTRTEQRRL RHKRIVKKIR ATNHDNRVVL MVIKSLNHIS VQAWDFSQNI VLASSSSLAL KLKNGNKDNA KLVGQDIADK LVKLKLTNV VFDTGGSKYH GRIAALAEAA RERGLNF

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Macromolecule #38: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae (strain ATCC 29342 / M129) (bacteria)
Strain: ATCC 29342 / M129
Molecular weightTheoretical: 13.870327 KDa
SequenceString:
MKKINKQALI DLVEQKQLKA YVPEFSAGDE VNVAIKLKEK EKVRIQNFTG TVLRRRGKGI SETFIVRKTT DGIPIEKNFQ IHNPNISIE LKRRGKVRRA YISYMRERSG KAAKIKERKQ

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Macromolecule #39: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 14.742431 KDa
SequenceString:
MRIKGGKQTR VRRKKWLKQA SGSFGTRHAS YKVAKQTVIQ AAKYAYRDRR NKKRDFRSLW ILRLNAALRE QGMTYSVFIN LLKKHNIEI NRKVLSELAI KEPSKFNLIV QKVKSEQPKA AKPAALGN

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Macromolecule #40: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 11.664824 KDa
SequenceString:
MHAIVVCGSK QYLVHENDTF FVEKLEAPVG KEIQLDKVLM LDEKIGAPYL EKARVVCVVE KHGLQRKVNV IKHISQKHHL KKYGHRQPY TKLKVVRFVH D

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Macromolecule #41: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 17.355402 KDa
SequenceString:
MIAFAKQFRV RISPQKARLV CQLIVGKKTA DAQNILSNTP KKAATLIAKL LNSAIANATN NHGMNGDALY VFECVANQGP SMKRTIPRA KGSSNMITKR SSNLVVKLSD NPNERQELIK QQKALVKKRV EGQQKAKMAR QKAVTSVVKA PSKTQGGVQK

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Macromolecule #42: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 25.89274 KDa
SequenceString: MDVTNVLLKP VLTEKVYFNQ MGETKKYVFV VNPKASKTRV KLAFELVYGI KPLKVNTLIR KPTTIRGGSR FPGLSKLEKL AVITLPKGI AISVTGEAPE KTDKPADKTT LKESTVKEIK DTKNSPEAVV KTAVEALQIK PTAAPVTTAP LQTVAVKVAK E VKEVKVEK ...String:
MDVTNVLLKP VLTEKVYFNQ MGETKKYVFV VNPKASKTRV KLAFELVYGI KPLKVNTLIR KPTTIRGGSR FPGLSKLEKL AVITLPKGI AISVTGEAPE KTDKPADKTT LKESTVKEIK DTKNSPEAVV KTAVEALQIK PTAAPVTTAP LQTVAVKVAK E VKEVKVEK PVKVEKPTKP AKVAKEAKTT KVAKETKAEK SVQTTKVAKE TKTEKSAKTT KTTATKTTKT KTTKKEVKK

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Macromolecule #43: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 12.461865 KDa
SequenceString:
MQRIKKGDKV VVITGKNKGG SGIVLKIMPA RQQAIVEGLN KVTRHKKKDQ TTKRAAKQST GKVQQEAPIF LSKLALFDQK AKQQTIGKI KYVMDPKTNK KTRVFKKSNN TL

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Macromolecule #44: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 11.527338 KDa
SequenceString:
MNNKYFLTKI DLQFFASKKG VGSTKNGRDS HAKRLGAKKA DGQMIRTGQI IYRQRGTRVY PGVNVGLGSD DTLFALSDGL VKYQKFGPK QGKTRVSVVK HKLDA

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Macromolecule #45: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 7.549902 KDa
SequenceString:
MAKKDQLTLR GPLYGNNRSH SKTITRRKWN VNLQPCKVKT ADGKTTRILV STRTLRTLKK HNRLS

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Macromolecule #46: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 13.003328 KDa
SequenceString:
MTVAKELRQK SSEELVKLVI KLKGELLEYR FKLAHGELDK PHLINQTRRL LATILTILTE RKLNWQEEQA KYKLLTKKTN EAAVNAWKQ HLEANKAKLL KSRAKREDAS KK

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Macromolecule #47: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 10.980657 KDa
SequenceString:
MKKDFHFPSQ SVSFKCASCS NSFTIESTLK QKEITIDICG KCHPFYIGEL TKQTVHGRAE KLSGKFNAGK AFLENKTPKK AKGKTEEYT KHRSLNEL

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Macromolecule #48: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 6.641897 KDa
SequenceString:
MAVQQRRSSK HRRDKRRSHD ALTAQALSVC QKCGKKKLFH RVCSCGMYGD LRVKKAY

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Macromolecule #49: 50S ribosomal protein L33 1

MacromoleculeName: 50S ribosomal protein L33 1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria) / Strain: ATCC 29342 / M129
Molecular weightTheoretical: 6.298612 KDa
SequenceString:
MAVKRSTRLG CNDCREINYL TFKNVKKNPE KLALNKFCSR CRKVVVHKEV KRK

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Macromolecule #50: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 50 / Number of copies: 1
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)
Molecular weightTheoretical: 940.9115 KDa
SequenceString: UACAAUAAGU UACUAAGGGC UUAUGGUGGA UGCCUUGGCA CUAAUAGGCG AUGAAGGACG UGUUAACCUG CGAUAAGCUU CGGGUAGGU GGUAAGAACC UCAGAUCCGG AGAUUUCCGA AUGGAGCAAU CCGGUAGUUG GAAACAGCUA UCAUUAAUUG A UGAAUAAA ...String:
UACAAUAAGU UACUAAGGGC UUAUGGUGGA UGCCUUGGCA CUAAUAGGCG AUGAAGGACG UGUUAACCUG CGAUAAGCUU CGGGUAGGU GGUAAGAACC UCAGAUCCGG AGAUUUCCGA AUGGAGCAAU CCGGUAGUUG GAAACAGCUA UCAUUAAUUG A UGAAUAAA UAGUCAAUUA AAGCAAUACG UGGUGAAGUG AAACAUCUCA GUAGCCACAG GAAAAGAAAA CGAAUGUGAU UC CGUGUGU AGUGGCGAGC GAAAGCGGAA CAGGCCAAAC UUAUCAUUAG AUAGGGGUUG UAGGGCUUGC AAUGUGGACU UGA AAACGA UAGAAGAAGC UGUUGGAAAG CAGCGCGCAA AAGGGUGAUA GCCCCGUAUU UGAAAUUGUU UUCAUACCUA GCGA GAUCC CUGAGUAGCU CGGAAAACGU UAUUUUGAGU GAAUCUGCCC AGACCAUUGG GUAAGCCUAA AUACUAAUUA GUGAC CGAU AGCGAAACAG UACCGUGAGG GAAAGGUGAA AAGAACCCAG AGAUGGGAGU GAAAUAGAUU CUGAAACCAU AUGCCU ACA ACGUGUCAGA GCACAUUAAU GUGUGAUGGC GUGCGUUUUG AAGUAUGAGC CGGCGAGUUA UGAUAGCAAG CGUUAGU UA ACCAGGAGAU GGGGAGCUGU AGCGAAAGCG AGUUUUAAAA GAGCGUUUGU UUGUUAUUAU AGACCCGAAA CGGGUUGA G CUAGUCAUGA GCAGGUUGAA GGUUGAGUAA CAUCAACUGG AGGACCGAAC CGACUCUCGU UGAAACGAUA GCGGAUGAC UUGUGAUUAG GGGUGAAAUU CCAAUCGAAA UCCGUGAUAG CUGGUUCUCG UCGAAAUAGC UUUAAGGCUA GCGUGAGAUC ACAAAUAAG UGGAGGUAAA GCUACUGAAU GUAUGAUGGC GCCACCUAGG CGUACUGAAU ACAAUUAAAC UCUGAAUGCC A UUUAUUUU AUUCUCGCAG UCAGACAGUG GGGGAUAAGC UUCAUUGUCA AGAGGGGAAG AGCCCAGAUC AUUAAAUAAG GU CCCCAAA AUAUACUAAG UGGAAAAGGA UGUGAAAGUG CUAAAACAGC AAGGAUGUUG GCUUAGAAGC AGCCAUCGUU UAA AGAGUG CGUAACAGCU CACUUGUCGA GUGUUUUUGC GCCGAAGAUG UAACGGGGCU AAGUAUAUUA CCGAAUUUAU GGAU AAGAU UUAUAUCUUG UGGUAGACGA GCGUUGUAUU GGAGUUGAAG UCAAAGCGUG AGCAUUGGUG GAUCCAAUAC AAGUG AGAA UGCCGGCAUG AGUAACGCUU GGGAGUGAGA AUCUCCCAAA CCGAUUGACU AAGGUUUCCU GGACCAGGGU CGUCCU UCC AGGGUUAGUC UGGACCUAAG CUGAGGCUGA AAAGCGUAGG CGAUGGACAA CAGGUUAAUA UUCCUGUACU UACAGUU AG ACUGAUGGAG UGACAAAGAA GGUUUUCCAC CCCCAUAAUU GGAUUUGGGG AUAAAUCAUA AGGUGGUACA AUAGGCAA A UCCGUUGUGC AUAACAUUGA GUGAUGAUGU CGAGUGAAUG AGUGAUCAAG UAGCGAAGGU GGUAUUAAUC AUGCUUUCA AGAAAAGCUU CUAGGGUUAA UCUAGCUGUA ACCAGUACCG AGAACGAACA CACGUAGUCA AGGAGAGGAU CCUAAGGUUA GCGAGUGAA CUAUAGCCAA GGAACUCUGC AAAUUAACCC CGUAAGUUAG CGAGAAGGGG UGCUUAUGUA AAAGUAAGCC G CAGUGAAG AACGAGGGGG GACUGUUUAA CUAAAACACA ACUCUAUGCC AAACCGUAAG GUGAUGUAUA UGGGGUGACA CC UGCCCAG UGCUGGAAGG UUAAAGAAGG AGGUUAGCGC AAGCGAAGCU UUUAACUGAA GCCCCAGUGA ACGGCGGCCG UAA CUAUAA CGGUCCUAAG GUAGCGAAAU UCCUAGUCGG GUAAAUUCCG UCCCGCUUGA AUGGUGUAAC CAUCUCUUGA CUGU CUCGG CUAUAGACUC GGUGAAAUCC AGGUACGGGU GAAGACACCC GUUAGGCGCA ACGGGACGGA AAGACCCCGU GAAGC UUUA CUGUAGCUUA AUAUUGAUCA GGACAUUAUC AUGUAGAGAA UAGGUAGGAG CAAUCGAUGC AAGUUCGCUA GGACUU GUU GAUGCGAAAG GUGGAAUACU ACCCUUGGUU GUGUGCUGUU CUAAUUGGUA ACUGUUAUCC AGUUUCAAGA CAGUGUU AG GUGGGCAGUU UGACUGGGGC GGUCGCCUCC UAAAAGGUAA CGGAGGCGUA CAAAGGUACC UUCAGUACGG UUGGAAAU C GUAUGUAGAG UGUAAUGGUG UAAGGGUGCU UGACUGUGAG ACAUACAGGU CGAACAGGUG AGAAAUCAGG UCAUAGUGA UCCGGUGGUC CAGUAUGGAA UGGCCAUCGC UCAACGGAUA AAAGCUACUC CGGGGAUAAC AGGCUGAUAC UGCCCAAGAG UUCAUAUCG ACGGCAGUGU UUGGCACCUC GAUGUCGACU CAUCUCAUCC UCGAGCUGAA GCAGGUUCGA AGGGUUCGGC U GUUCGCCG AUUAAAGAGA UACGUGAGUU GGGUUCAAAC CGUCGUGAGA CAGGUUGGUC CCUAUCUAUU GUGCCCGUAG GA AGAUUGA AGAGUGUUGC UUCUAGUACG AGAGGACCGA AGCGAGGACA CCUCUUAUGC UCCAGUUGUA GCGCCAGCUG CAC CGCUGG GUAGUAACGU GUCUAUUAGA UAAACGCUGA AAGCAUCUAA GUGUGAAACU AUCUCAAAGA UUAAUCUUCC CAUU UCGCA AGAAAGUAAG AGCCGUCAAA GACGAUGACG UUGAUAGGUU ACAGGUGUAA GCAUAGUGAU AUGUUGAGCU GAGUA AUAC UAAUUGCUCG AGGACUUAUU GGA

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Macromolecule #51: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 51 / Number of copies: 1
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)
Molecular weightTheoretical: 34.796676 KDa
SequenceString:
UUUGGUGCCC AUGUCGCUGU GGAAACACCU GGUUCCAUUU CGAACCCAGC AGUUAAGCAC AGUGGAGCCG AAUGUAGCUG UUUCAGUGA GAAUAGGAAA GCACCAAGC

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Macromolecule #52: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)
Molecular weightTheoretical: 491.243625 KDa
SequenceString: UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA ...String:
UUUUUCUGAG AGUUUGAUCC UGGCUCAGGA UUAACGCUGG CGGCAUGCCU AAUACAUGCA AGUCGAUCGA AAGUAGUAAU ACUUUAGAG GCGAACGGGU GAGUAACACG UAUCCAAUCU ACCUUAUAAU GGGGGAUAAC UAGUUGAAAG ACUAGCUAAU A CCGCAUAA GAACUUUGGU UCGCAUGAAU CAAAGUUGAA AGGACCUGCA AGGGUUCGUU AUUUGAUGAG GGUGCGCCAU AU CAGCUAG UUGGUGGGGU AACGGCCUAC CAAGGCAAUG ACGUGUAGCU AUGCUGAGAA GUAGAAUAGC CACAAUGGGA CUG AGACAC GGCCCAUACU CCUACGGGAG GCAGCAGUAG GGAAUUUUUC ACAAUGAGCG AAAGCUUGAU GGAGCAAUGC CGCG UGAAC GAUGAAGGUC UUUAAGAUUG UAAAGUUCUU UUAUUUGGGA AGAAUGACUU UAGCAGGUAA UGGCUAGAGU UUGAC UGUA CCAUUUUGAA UAAGUGACGA CUAACUAUGU GCCAGCAGUC GCGGUAAUAC AUAGGUCGCA AGCGUUAUCC GGAUUU AUU GGGCGUAAAG CAAGCGCAGG CGGAUUGAAA AGUCUGGUGU UAAAGGCAGC UGCUUAACAG UUGUAUGCAU UGGAAAC UA UUAAUCUAGA GUGUGGUAGG GAGUUUUGGA AUUUCAUGUG GAGCGGUGAA AUGCGUAGAU AUAUGAAGGA ACACCAGU G GCGAAGGCGA AAACUUAGGC CAUUACUGAC GCUUAGGCUU GAAAGUGUGG GGAGCAAAUA GGAUUAGAUA CCCUAGUAG UCCACACCGU AAACGAUAGA UACUAGCUGU CGGGGCGAUC CCCUCGGUAG UGAAGUUAAC ACAUUAAGUA UCUCGCCUGG GUAGUACAU UCGCAAGAAU GAAACUCAAA CGGAAUUGAC GGGGACCCGC ACAAGUGGUG GAGCAUGUUG CUUAAUUCGA C GGUACACG AAAAACCUUA CCUAGACUUG ACAUCCUUGG CAAAGUUAUG GAAACAUAAU GGAGGUUAAC CGAGUGACAG GU GGUGCAU GGUUGUCGUC AGCUCGUGUC GUGAGAUGUU GGGUUAAGUC CCGCAACGAG CGCAACCCUU AUCGUUAGUU ACA UUGUCU AGCGAGACUG CUAAUGCAAA UUGGAGGAAG GAAGGGAUGA CGUCAAAUCA UCAUGCCCCU UAUGUCUAGG GCUG CAAAC GUGCUACAAU GGCCAAUACA AACAGUCGCC AGCUUGUAAA AGUGAGCAAA UCUGUAAAGU UGGUCUCAGU UCGGA UUGA GGGCUGCAAU UCGUCCUCAU GAAGUCGGAA UCACUAGUAA UCGCGAAUCA GCUAUGUCGC GGUGAAUACG UUCUCG GGU CUUGUACACA CCGCCCGUCA AACUAUGAAA GCUGGUAAUA UUUAAAAACG UGUUGCUAAC CAUUAGGAAG CGCAUGU CA AGGAUAGCAC CGGUGAUUGG AGUUAAGUCG UAACAAGGUA CCCCUACGAG AACGUGGGGG UGGAUCACCU CCUUUCUA

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Macromolecule #53: tRNA-Phe

MacromoleculeName: tRNA-Phe / type: rna / ID: 53 / Number of copies: 2
Source (natural)Organism: Mycoplasma pneumoniae M129 (bacteria)
Molecular weightTheoretical: 24.470521 KDa
SequenceString:
GGUCUUGUAG CUCAGUCGGU AGAGCAACGG UCUGAAGAAC CGUGUGUCGG CAGUUCGAUU CUGCCCGAGA CCACCA

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE-PROPANE / Instrument: HOMEMADE PLUNGER
Details: back-side blotting for 2-3 seconds before plunging using a manual plunger without an environmental chamber.
DetailsMycoplasma pneumoniae M129 cells were grown on gold Quantifoil grids at 37 Celsius. Treatment with spectinomycin was performed for 15 to 20 minutes before plunge freezing.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.75 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Detector mode: COUNTING / Average electron dose: 3.2 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

ExtractionNumber tomograms: 70 / Number images used: 13418 / Software: (Name: PyTom, Warp (ver. 3.0.7 beta or 3.0.9))
CTF correctionSoftware - Name: RELION (ver. 3.0.8)
Final 3D classificationSoftware - Name: RELION (ver. 3.0.8)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0.8)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 13.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0.8) / Number subtomograms used: 1119
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChain

7ooc


7ood


4v7c

chain_id: 7

4v7c

chain_id: 8
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-7pia:
70S ribosome with A/P- and P/E-site tRNAs in spectinomycin-treated Mycoplasma pneumoniae cells

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