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- EMDB-13375: Core human replisome -

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Basic information

Entry
Database: EMDB / ID: EMD-13375
TitleCore human replisome
Map dataConsensus refinement core human replisome
Sample
  • Complex: Core human replisome
    • Protein or peptide: x 17 types
    • DNA: x 2 types
  • Ligand: x 4 types
KeywordsDNA replication / helicase / CMG / TIMELESS / TIPIN / CLASPIN / AND-1 / EPSILON / POLYMERASE / REPLISOME / REPLICATION
Function / homology
Function and homology information


cellular response to bleomycin / DNA secondary structure binding / Switching of origins to a post-replicative state / detection of abiotic stimulus / replication fork arrest / regulation of nuclear cell cycle DNA replication / Unwinding of DNA / cell cycle phase transition / cellular response to cisplatin / DNA replication initiation ...cellular response to bleomycin / DNA secondary structure binding / Switching of origins to a post-replicative state / detection of abiotic stimulus / replication fork arrest / regulation of nuclear cell cycle DNA replication / Unwinding of DNA / cell cycle phase transition / cellular response to cisplatin / DNA replication initiation / epsilon DNA polymerase complex / GINS complex / DNA strand elongation involved in mitotic DNA replication / mitotic DNA replication preinitiation complex assembly / nuclear origin of replication recognition complex / cellular response to hydroxyurea / alpha DNA polymerase:primase complex / mitotic DNA replication / anaphase-promoting complex binding / CMG complex / DNA replication checkpoint signaling / single-stranded DNA 3'-5' DNA exonuclease activity / MCM complex / DNA replication preinitiation complex / regulation of phosphorylation / mitotic DNA replication checkpoint signaling / replication fork protection complex / double-strand break repair via break-induced replication / mitotic DNA replication initiation / mitotic intra-S DNA damage checkpoint signaling / regulation of DNA-templated DNA replication initiation / positive regulation of double-strand break repair / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / inner cell mass cell proliferation / nucleotide-excision repair, DNA gap filling / DNA replication proofreading / DNA strand elongation involved in DNA replication / branching morphogenesis of an epithelial tube / cochlea development / DNA synthesis involved in DNA repair / G1/S-Specific Transcription / activation of protein kinase activity / DNA unwinding involved in DNA replication / replication fork processing / leading strand elongation / Apoptotic cleavage of cellular proteins / nuclear replication fork / mitotic G2 DNA damage checkpoint signaling / DNA replication origin binding / positive regulation of double-strand break repair via homologous recombination / Activation of the pre-replicative complex / DNA replication initiation / PCNA-Dependent Long Patch Base Excision Repair / cellular response to interleukin-4 / embryonic organ development / error-prone translesion synthesis / Activation of ATR in response to replication stress / response to UV / DNA helicase activity / base-excision repair, gap-filling / Gap-filling DNA repair synthesis and ligation in GG-NER / ciliary basal body / DNA damage checkpoint signaling / helicase activity / Assembly of the pre-replicative complex / morphogenesis of an epithelium / Recognition of DNA damage by PCNA-containing replication complex / Termination of translesion DNA synthesis / lung development / HDR through Homologous Recombination (HRR) / regulation of circadian rhythm / Dual Incision in GG-NER / DNA-templated DNA replication / Dual incision in TC-NER / Orc1 removal from chromatin / Gap-filling DNA repair synthesis and ligation in TC-NER / circadian rhythm / G1/S transition of mitotic cell cycle / cellular response to xenobiotic stimulus / nucleosome assembly / mitotic cell cycle / site of double-strand break / single-stranded DNA binding / Processing of DNA double-strand break ends / 4 iron, 4 sulfur cluster binding / histone binding / peptidyl-serine phosphorylation / DNA helicase / DNA replication / cell population proliferation / chromosome, telomeric region / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nuclear body / Ub-specific processing proteases / cell division / intracellular membrane-bounded organelle / DNA repair / nucleotide binding / negative regulation of DNA-templated transcription
Similarity search - Function
DNA polymerase epsilon subunit B, N-terminal / DNA polymerases epsilon N terminal / Claspin / Timeless, C-terminal / Timeless PAB domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / Timeless / Timeless, N-terminal ...DNA polymerase epsilon subunit B, N-terminal / DNA polymerases epsilon N terminal / Claspin / Timeless, C-terminal / Timeless PAB domain / Chromosome segregation in meiosis protein 3 / TIPIN/Csm3/Swi3 / Replication Fork Protection Component Swi3 / Timeless / Timeless, N-terminal / Timeless protein / DNA polymerase epsilon, subunit B / : / DNA polymerase alpha-binding protein Ctf4, C-terminal domain / Minichromosome loss protein Mcl1, middle region / Minichromosome loss protein, Mcl1, middle region / DNA polymerase epsilon, catalytic subunit A, C-terminal / DNA polymerase epsilon catalytic subunit / Domain of unknown function (DUF1744) / DUF1744 / CDC45 family / DNA replication complex GINS protein Psf2 / CDC45-like protein / GINS complex, subunit Psf1 / GINS complex, subunit Psf3 / GINS complex, subunit Psf3 superfamily / DNA replication complex GINS protein SLD5, C-terminal / GINS, helical bundle-like domain superfamily / GINS complex protein Sld5, alpha-helical domain / DNA replication complex GINS protein SLD5 C-terminus / GINS complex subunit Sld5 / GINS subunit, domain A / GINS complex protein helical bundle domain / MCM4, winged helix domain / DNA polymerase alpha/delta/epsilon, subunit B / DNA replication licensing factor Mcm5 / DNA polymerase alpha/epsilon subunit B / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm6 / DNA replication licensing factor Mcm7 / Mcm6, C-terminal winged-helix domain / MCM6 C-terminal winged-helix domain / DNA replication licensing factor Mcm2 / Mini-chromosome maintenance protein 2 / Mini-chromosome maintenance, conserved site / MCM family signature. / MCM N-terminal domain / MCM N-terminal domain / MCM OB domain / MCM OB domain / Mini-chromosome maintenance protein / MCM, AAA-lid domain / MCM P-loop domain / MCM AAA-lid domain / MCM family domain profile. / minichromosome maintenance proteins / MCM domain / Anaphase-promoting complex subunit 4, WD40 domain / Anaphase-promoting complex subunit 4 WD40 domain / DNA polymerase family B, thumb domain / HMG boxes A and B DNA-binding domains profile. / high mobility group / High mobility group box domain / High mobility group box domain superfamily / DNA polymerase family B / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA-directed DNA polymerase, family B, multifunctional domain / DNA polymerase, palm domain superfamily / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / Ribonuclease H superfamily / Ribonuclease H-like superfamily / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / DNA/RNA polymerase superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA polymerase epsilon subunit 2 / DNA polymerase epsilon catalytic subunit A / DNA replication licensing factor MCM6 ...Cell division control protein 45 homolog / WD repeat and HMG-box DNA-binding protein 1 / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM5 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM2 / DNA polymerase epsilon subunit 2 / DNA polymerase epsilon catalytic subunit A / DNA replication licensing factor MCM6 / DNA replication complex GINS protein PSF1 / DNA replication complex GINS protein SLD5 / DNA replication complex GINS protein PSF3 / TIMELESS-interacting protein / Claspin / Protein timeless homolog / DNA replication complex GINS protein PSF2
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsJones MJ / Yeeles JTP
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_1201/12 United Kingdom
CitationJournal: Nature / Year: 2021
Title: A conserved mechanism for regulating replisome disassembly in eukaryotes.
Authors: Michael Jenkyn-Bedford / Morgan L Jones / Yasemin Baris / Karim P M Labib / Giuseppe Cannone / Joseph T P Yeeles / Tom D Deegan /
Abstract: Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily ...Replisome disassembly is the final step of eukaryotic DNA replication and is triggered by ubiquitylation of the CDC45-MCM-GINS (CMG) replicative helicase. Despite being driven by evolutionarily diverse E3 ubiquitin ligases in different eukaryotes (SCF in budding yeast, CUL2 in metazoa), replisome disassembly is governed by a common regulatory principle, in which ubiquitylation of CMG is suppressed before replication termination, to prevent replication fork collapse. Recent evidence suggests that this suppression is mediated by replication fork DNA. However, it is unknown how SCF and CUL2 discriminate terminated from elongating replisomes, to selectively ubiquitylate CMG only after termination. Here we used cryo-electron microscopy to solve high-resolution structures of budding yeast and human replisome-E3 ligase assemblies. Our structures show that the leucine-rich repeat domains of Dia2 and LRR1 are structurally distinct, but bind to a common site on CMG, including the MCM3 and MCM5 zinc-finger domains. The LRR-MCM interaction is essential for replisome disassembly and, crucially, is occluded by the excluded DNA strand at replication forks, establishing the structural basis for the suppression of CMG ubiquitylation before termination. Our results elucidate a conserved mechanism for the regulation of replisome disassembly in eukaryotes, and reveal a previously unanticipated role for DNA in preserving replisome integrity.
History
DepositionAug 11, 2021-
Header (metadata) releaseNov 10, 2021-
Map releaseNov 10, 2021-
UpdateOct 18, 2023-
Current statusOct 18, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0113
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.0113
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7pfo
  • Surface level: 0.0113
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_13375.png

Downloads & links

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Map

FileDownload / File: emd_13375.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationConsensus refinement core human replisome
Voxel sizeX=Y=Z: 1.07 Å
Density
Contour LevelBy AUTHOR: 0.0113 / Movie #1: 0.0113
Minimum - Maximum-0.016569365 - 0.061714876
Average (Standard dev.)-0.00024775672 (±0.0022362303)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 470.80002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z440440440
origin x/y/z0.0000.0000.000
length x/y/z470.800470.800470.800
α/β/γ90.00090.00090.000
start NX/NY/NZ7810892
NX/NY/NZ127111124
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS440440440
D min/max/mean-0.0170.062-0.000

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Supplemental data

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Mask #1

Fileemd_13375_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Local resolution filtered consensus refinement core human replisome

Fileemd_13375_additional_1.map
AnnotationLocal resolution filtered consensus refinement core human replisome
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Additional map: Composite map generate using PHENIX combine focussed maps. Combining consensus...

Fileemd_13375_additional_2.map
AnnotationComposite map generate using PHENIX combine_focussed_maps. Combining consensus refinement, AND1/CDC45/GINS and POLE/CDC45/GINS multibodies.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Consensus refinement core human replisome half map 2

Fileemd_13375_half_map_1.map
AnnotationConsensus refinement core human replisome half map 2
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: Consensus refinement core human replisome half map 1

Fileemd_13375_half_map_2.map
AnnotationConsensus refinement core human replisome half map 1
Projections & Slices
AxesZYX

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Sample components

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Entire : Core human replisome

EntireName: Core human replisome
Components
  • Complex: Core human replisome
    • Protein or peptide: DNA polymerase epsilon catalytic subunit A
    • Protein or peptide: DNA polymerase epsilon subunit 2
    • Protein or peptide: DNA replication licensing factor MCM4
    • Protein or peptide: DNA replication licensing factor MCM6
    • Protein or peptide: DNA replication licensing factor MCM7
    • Protein or peptide: DNA replication licensing factor MCM3
    • Protein or peptide: DNA replication licensing factor MCM2
    • Protein or peptide: DNA replication licensing factor MCM5
    • Protein or peptide: DNA replication complex GINS protein PSF1
    • Protein or peptide: DNA replication complex GINS protein PSF2
    • Protein or peptide: DNA replication complex GINS protein PSF3
    • Protein or peptide: DNA replication complex GINS protein SLD5
    • Protein or peptide: Cell division control protein 45 homolog
    • Protein or peptide: Protein timeless homolog
    • Protein or peptide: TIMELESS-interacting protein
    • DNA: Leading strand DNA
    • Protein or peptide: WD repeat and HMG-box DNA-binding protein 1
    • DNA: Lagging Strand DNA
    • Protein or peptide: Claspin
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: SULFATE ION

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Supramolecule #1: Core human replisome

SupramoleculeName: Core human replisome / type: complex / ID: 1 / Parent: 0
Macromolecule list: #8, #7, #3, #5-#6, #2, #1, #4, #10-#13, #9, #15-#17, #14, #18-#19
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: DNA replication licensing factor MCM2

MacromoleculeName: DNA replication licensing factor MCM2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 102.034102 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ...String:
MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDA YEAEGLALDD EDVEELTASQ REAAERAMRQ RDREAGRGLG RMRRGLLYDS DEEDEERPAR KRRQVERATE D GEEDEEMI ESIENLEDLK GHSVREWVSM AGPRLEIHHR FKNFLRTHVD SHGHNVFKER ISDMCKENRE SLVVNYEDLA AR EHVLAYF LPEAPAELLQ IFDEAALEVV LAMYPKYDRI TNHIHVRISH LPLVEELRSL RQLHLNQLIR TSGVVTSCTG VLP QLSMVK YNCNKCNFVL GPFCQSQNQE VKPGSCPECQ SAGPFEVNME ETIYQNYQRI RIQESPGKVA AGRLPRSKDA ILLA DLVDS CKPGDEIELT GIYHNNYDGS LNTANGFPVF ATVILANHVA KKDNKVAVGE LTDEDVKMIT SLSKDQQIGE KIFAS IAPS IYGHEDIKRG LALALFGGEP KNPGGKHKVR GDINVLLCGD PGTAKSQFLK YIEKVSSRAI FTTGQGASAV GLTAYV QRH PVSREWTLEA GALVLADRGV CLIDEFDKMN DQDRTSIHEA MEQQSISISK AGIVTSLQAR CTVIAAANPI GGRYDPS LT FSENVDLTEP IISRFDILCV VRDTVDPVQD EMLARFVVGS HVRHHPSNKE EEGLANGSAA EPAMPNTYGV EPLPQEVL K KYIIYAKERV HPKLNQMDQD KVAKMYSDLR KESMATGSIP ITVRHIESMI RMAEAHARIH LRDYVIEDDV NMAIRVMLE SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV AEQVTYQRNR FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMN KFSHDLKRKM ILQQF

UniProtKB: DNA replication licensing factor MCM2

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Macromolecule #2: DNA replication licensing factor MCM3

MacromoleculeName: DNA replication licensing factor MCM3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 91.110852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF ...String:
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDA TYAKQYEEFY VGLEGSFGSK HVSPRTLTSC FLSCVVCVEG IVTKCSLVRP KVVRSVHYCP ATKKTIERRY S DLTTLVAF PSSSVYPTKD EENNPLETEY GLSVYKDHQT ITIQEMPEKA PAGQLPRSVD VILDDDLVDK AKPGDRVQVV GT YRCLPGK KGGYTSGTFR TVLIACNVKQ MSKDAQPSFS AEDIAKIKKF SKTRSKDIFD QLAKSLAPSI HGHDYVKKAI LCL LLGGVE RDLENGSHIR GDINILLIGD PSVAKSQLLR YVLCTAPRAI PTTGRGSSGV GLTAAVTTDQ ETGERRLEAG AMVL ADRGV VCIDEFDKMS DMDRTAIHEV MEQGRVTIAK AGIHARLNAR CSVLAAANPV YGRYDQYKTP MENIGLQDSL LSRFD LLFI MLDQMDPEQD REISDHVLRM HRYRAPGEQD GDAMPLGSAV DILATDDPNF SQEDQQDTQI YEKHDNLLHG TKKKKE KMV SAAFMKKYIH VAKIIKPVLT QESATYIAEE YSRLRSQDSM SSDTARTSPV TARTLETLIR LATAHAKARM SKTVDLQ DA EEAVELVQYA YFKKVLEKEK KRKKRSEDES ETEDEEEKSQ EDQEQKRKRR KTRQPDAKDG DSYDPYDFSD TEEEMPQV H TPKTADSQET KESQKVELSE SRLKAFKVAL LDVFREAHAQ SIGMNRLTES INRDSEEPFS SVEIQAALSK MQDDNQVMV SEGIIFLI

UniProtKB: DNA replication licensing factor MCM3

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Macromolecule #3: DNA replication licensing factor MCM4

MacromoleculeName: DNA replication licensing factor MCM4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 96.684852 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR ...String:
MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSP LTYGTPSSRV EGTPRSGVRG TPVRQRPDLG SAQKGLQVDL QSDGAAAEDI VASEQSLGQK LVIWGTDVNV A ACKENFQR FLQRFIDPLA KEEENVGIDI TEPLYMQRLG EINVIGEPFL NVNCEHIKSF DKNLYRQLIS YPQEVIPTFD MA VNEIFFD RYPDSILEHQ IQVRPFNALK TKNMRNLNPE DIDQLITISG MVIRTSQLIP EMQEAFFQCQ VCAHTTRVEM DRG RIAEPS VCGRCHTTHS MALIHNRSLF SDKQMIKLQE SPEDMPAGQT PHTVILFAHN DLVDKVQPGD RVNVTGIYRA VPIR VNPRV SNVKSVYKTH IDVIHYRKTD AKRLHGLDEE AEQKLFSEKR VELLKELSRK PDIYERLASA LAPSIYEHED IKKGI LLQL FGGTRKDFSH TGRGKFRAEI NILLCGDPGT SKSQLLQYVY NLVPRGQYTS GKGSSAVGLT AYVMKDPETR QLVLQT GAL VLSDNGICCI DEFDKMNEST RSVLHEVMEQ QTLSIAKAGI ICQLNARTSV LAAANPIESQ WNPKKTTIEN IQLPHTL LS RFDLIFLLLD PQDEAYDRRL AHHLVALYYQ SEEQAEEELL DMAVLKDYIA YAHSTIMPRL SEEASQALIE AYVDMRKI G SSRGMVSAYP RQLESLIRLA EAHAKVRLSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATSRKRKEE LAEALKKLIL SKGKTPALKY QQLFEDIRGQ SDIAITKDMF EEALRALADD DFLTVTGKTV RLL

UniProtKB: DNA replication licensing factor MCM4

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Macromolecule #4: DNA replication licensing factor MCM5

MacromoleculeName: DNA replication licensing factor MCM5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 82.406633 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI ...String:
MSGFDDPGIF YSDSFGGDAQ ADEGQARKSQ LQRRFKEFLR QYRVGTDRTG FTFKYRDELK RHYNLGEYWI EVEMEDLASF DEDLADYLY KQPAEHLQLL EEAAKEVADE VTRPRPSGEE VLQDIQVMLK SDASPSSIRS LKSDMMSHLV KIPGIIIAAS A VRAKATRI SIQCRSCRNT LTNIAMRPGL EGYALPRKCN TDQAGRPKCP LDPYFIMPDK CKCVDFQTLK LQELPDAVPH GE MPRHMQL YCDRYLCDKV VPGNRVTIMG IYSIKKFGLT TSRGRDRVGV GIRSSYIRVL GIQVDTDGSG RSFAGAVSPQ EEE EFRRLA ALPNVYEVIS KSIAPSIFGG TDMKKAIACL LFGGSRKRLP DGLTRRGDIN LLMLGDPGTA KSQLLKFVEK CSPI GVYTS GKGSSAAGLT ASVMRDPSSR NFIMEGGAMV LADGGVVCID EFDKMREDDR VAIHEAMEQQ TISIAKAGIT TTLNS RCSV LAAANSVFGR WDETKGEDNI DFMPTILSRF DMIFIVKDEH NEERDVMLAK HVITLHVSAL TQTQAVEGEI DLAKLK KFI AYCRVKCGPR LSAEAAEKLK NRYIIMRSGA RQHERDSDRR SSIPITVRQL EAIVRIAEAL SKMKLQPFAT EADVEEA LR LFQVSTLDAA LSGTLSGVEG FTSQEDQEML SRIEKQLKRR FAIGSQVSEH SIIKDFTKQK YPEHAIHKVL QLMLRRGE I QHRMQRKVLY RLK

UniProtKB: DNA replication licensing factor MCM5

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Macromolecule #5: DNA replication licensing factor MCM6

MacromoleculeName: DNA replication licensing factor MCM6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 93.010273 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV ...String:
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPY LCRALKTFVK DRKEIPLAKD FYVAFQDLPT RHKIRELTSS RIGLLTRISG QVVRTHPVHP ELVSGTFLCL D CQTVIRDV EQQFKYTQPN ICRNPVCANR RRFLLDTNKS RFVDFQKVRI QETQAELPRG SIPRSLEVIL RAEAVESAQA GD KCDFTGT LIVVPDVSKL STPGARAETN SRVSGVDGYE TEGIRGLRAL GVRDLSYRLV FLACCVAPTN PRFGGKELRD EEQ TAESIK NQMTVKEWEK VFEMSQDKNL YHNLCTSLFP TIHGNDEVKR GVLLMLFGGV PKTTGEGTSL RGDINVCIVG DPST AKSQF LKHVEEFSPR AVYTSGKASS AAGLTAAVVR DEESHEFVIE AGALMLADNG VCCIDEFDKM DVRDQVAIHE AMEQQ TISI TKAGVKATLN ARTSILAAAN PISGHYDRSK SLKQNINLSA PIMSRFDLFF ILVDECNEVT DYAIARRIVD LHSRIE ESI DRVYSLDDIR RYLLFARQFK PKISKESEDF IVEQYKHLRQ RDGSGVTKSS WRITVRQLES MIRLSEAMAR MHCCDEV QP KHVKEAFRLL NKSIIRVETP DVNLDQEEEI QMEVDEGAGG INGHADSPAP VNGINGYNED INQESAPKAS LRLGFSEY C RISNLIVLHL RKVEEEEDES ALKRSELVNW YLKEIESEID SEEELINKKR IIEKVIHRLT HYDHVLIELT QAGLKGSTE GSESYEEDPY LVVNPNYLLE D

UniProtKB: DNA replication licensing factor MCM6

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Macromolecule #6: DNA replication licensing factor MCM7

MacromoleculeName: DNA replication licensing factor MCM7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 81.411875 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT ...String:
MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYK EREVVNKDVL DVYIEHRLMM EQRSRDPGMV RSPQNQYPAE LMRRFELYFQ GPSSNKPRVI REVRADSVGK L VTVRGIVT RVSEVKPKMV VATYTCDQCG AETYQPIQSP TFMPLIMCPS QECQTNRSGG RLYLQTRGSR FIKFQEMKMQ EH SDQVPVG NIPRSITVLV EGENTRIAQP GDHVSVTGIF LPILRTGFRQ VVQGLLSETY LEAHRIVKMN KSEDDESGAG ELT REELRQ IAEEDFYEKL AASIAPEIYG HEDVKKALLL LLVGGVDQSP RGMKIRGNIN ICLMGDPGVA KSQLLSYIDR LAPR SQYTT GRGSSGVGLT AAVLRDSVSG ELTLEGGALV LADQGVCCID EFDKMAEADR TAIHEVMEQQ TISIAKAGIL TTLNA RCSI LAAANPAYGR YNPRRSLEQN IQLPAALLSR FDLLWLIQDR PDRDNDLRLA QHITYVHQHS RQPPSQFEPL DMKLMR RYI AMCREKQPMV PESLADYITA AYVEMRREAW ASKDATYTSA RTLLAILRLS TALARLRMVD VVEKEDVNEA IRLMEMS KD SLLGDKGQTA RTQRPADVIF ATVRELVSGG RSVRFSEAEQ RCVSRGFTPA QFQAALDEYE ELNVWQVNAS RTRITFV

UniProtKB: DNA replication licensing factor MCM7

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Macromolecule #7: DNA polymerase epsilon subunit 2

MacromoleculeName: DNA polymerase epsilon subunit 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 59.600887 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES G SKFQLKTI ...String:
MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES G SKFQLKTI ETLLGSTTKI GDAIVLGMIT QLKEGKFFLE DPTGTVQLDL SKAQFHSGLY TEACFVLAEG WFEDQVFHVN AF GFPPTEP SSTTRAYYGN INFFGGPSNT SVKTSAKLKQ LEEENKDAMF VFLSDVWLDQ VEVLEKLRIM FAGYSPAPPT CFI LCGNFS SAPYGKNQVQ ALKDSLKTLA DIICEYPDIH QSSRFVFVPG PEDPGFGSIL PRPPLAESIT NEFRQRVPFS VFTT NPCRI QYCTQEITVF REDLVNKMCR NCVRFPSSNL AIPNHFVKTI LSQGHLTPLP LYVCPVYWAY DYALRVYPVP DLLVI ADKY DPFTTTNTEC LCINPGSFPR SGFSFKVFYP SNKTVEDSKL QGF

UniProtKB: DNA polymerase epsilon subunit 2

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Macromolecule #8: DNA polymerase epsilon catalytic subunit A

MacromoleculeName: DNA polymerase epsilon catalytic subunit A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed DNA polymerase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 261.855266 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSLRSGGRRR ADPGADGEAS RDDGATSSVS ALKRLERSQW TDKMDLRFGF ERLKEPGEKT GWLINMHPTE ILDEDKRLGS AVDYYFIQD DGSRFKVALP YKPYFYIATR KGCEREVSSF LSKKFQGKIA KVETVPKEDL DLPNHLVGLK RNYIRLSFHT V EDLVKVRK ...String:
MSLRSGGRRR ADPGADGEAS RDDGATSSVS ALKRLERSQW TDKMDLRFGF ERLKEPGEKT GWLINMHPTE ILDEDKRLGS AVDYYFIQD DGSRFKVALP YKPYFYIATR KGCEREVSSF LSKKFQGKIA KVETVPKEDL DLPNHLVGLK RNYIRLSFHT V EDLVKVRK EISPAVKKNR EQDHASDAYT ALLSSVLQRG GVITDEEETS KKIADQLDNI VDMREYDVPY HIRLSIDLKI HV AHWYNVR YRGNAFPVEI TRRDDLVERP DPVVLAFDIE TTKLPLKFPD AETDQIMMIS YMIDGQGYLI TNREIVSEDI EDF EFTPKP EYEGPFCVFN EPDEAHLIQR WFEHVQETKP TIMVTYNGDF FDWPFVEARA AVHGLSMQQE IGFQKDSQGE YKAP QCIHM DCLRWVKRDS YLPVGSHNLK AAAKAKLGYD PVELDPEDMC RMATEQPQTL ATYSVSDAVA TYYLYMKYVH PFIFA LCTI IPMEPDEVLR KGSGTLCEAL LMVQAFHANI IFPNKQEQEF NKLTDDGHVL DSETYVGGHV EALESGVFRS DIPCRF RMN PAAFDFLLQR VEKTLRHALE EEEKVPVEQV TNFEEVCDEI KSKLASLKDV PSRIECPLIY HLDVGAMYPN IILTNRL QP SAMVDEATCA ACDFNKPGAN CQRKMAWQWR GEFMPASRSE YHRIQHQLES EKFPPLFPEG PARAFHELSR EEQAKYEK R RLADYCRKAY KKIHITKVEE RLTTICQREN SFYVDTVRAF RDRRYEFKGL HKVWKKKLSA AVEVGDAAEV KRCKNMEVL YDSLQLAHKC ILNSFYGYVM RKGARWYSME MAGIVCFTGA NIITQARELI EQIGRPLELD TDGIWCVLPN SFPENFVFKT TNVKKPKVT ISYPGAMLNI MVKEGFTNDQ YQELAEPSSL TYVTRSENSI FFEVDGPYLA MILPASKEEG KKLKKRYAVF N EDGSLAEL KGFEVKRRGE LQLIKIFQSS VFEAFLKGST LEEVYGSVAK VADYWLDVLY SKAANMPDSE LFELISENRS MS RKLEDYG EQKSTSISTA KRLAEFLGDQ MVKDAGLSCR YIISRKPEGS PVTERAIPLA IFQAEPTVRK HFLRKWLKSS SLQ DFDIRA ILDWDYYIER LGSAIQKIIT IPAALQQVKN PVPRVKHPDW LHKKLLEKND VYKQKKISEL FTLEGRRQVT MAEA SEDSP RPSAPDMEDF GLVKLPHPAA PVTVKRKRVL WESQEESQDL TPTVPWQEIL GQPPALGTSQ EEWLVWLRFH KKKWQ LQAR QRLARRKRQR LESAEGVLRP GAIRDGPATG LGSFLRRTAR SILDLPWQIV QISETSQAGL FRLWALVGSD LHCIRL SIP RVFYVNQRVA KAEEGASYRK VNRVLPRSNM VYNLYEYSVP EDMYQEHINE INAELSAPDI EGVYETQVPL LFRALVH LG CVCVVNKQLV RHLSGWEAET FALEHLEMRS LAQFSYLEPG SIRHIYLYHH AQAHKALFGI FIPSQRRASV FVLDTVRS N QMPSLGALYS AEHGLLLEKV GPELLPPPKH TFEVRAETDL KTICRAIQRF LLAYKEERRG PTLIAVQSSW ELKRLASEI PVLEEFPLVP ICVADKINYG VLDWQRHGAR RMIRHYLNLD TCLSQAFEMS RYFHIPIGNL PEDISTFGSD LFFARHLQRH NHLLWLSPT ARPDLGGKEA DDNCLVMEFD DQATVEINSS GCYSTVCVEL DLQNLAVNTI LQSHHVNDME GADSMGISFD V IQQASLED MITGGQAASA PASYDETALC SNTFRILKSM VVGWVKEITQ YHNIYADNQV MHFYRWLRSP SSLLHDPALH RT LHNMMKK LFLQLIAEFK RLGSSVIYAN FNRIILCTKK RRVEDAIAYV EYITSSIHSK ETFHSLTISF SRCWEFLLWM DPS NYGGIK GKVSSRIHCG LQDSQKAGGA EDEQENEDDE EERDGEEEEE AEESNVEDLL ENNWNILQFL PQAASCQNYF LMIV SAYIV AVYHCMKDGL RRSAPGSTPV RRRGASQLSQ EAEGAVGALP GMITFSQDYV ANELTQSFFT ITQKIQKKVT GSRNS TELS EMFPVLPGSH LLLNNPALEF IKYVCKVLSL DTNITNQVNK LNRDLLRLVD VGEFSEEAQF RDPCRSYVLP EVICRS CNF CRDLDLCKDS SFSEDGAVLP QWLCSNCQAP YDSSAIEMTL VEVLQKKLMA FTLQDLVCLK CRGVKETSMP VYCSCAG DF ALTIHTQVFM EQIGIFRNIA QHYGMSYLLE TLEWLLQKNP QLGH

UniProtKB: DNA polymerase epsilon catalytic subunit A

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Macromolecule #9: Cell division control protein 45 homolog

MacromoleculeName: Cell division control protein 45 homolog / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 66.016891 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV ...String:
MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQP DEDTIFFVCD THRPVNVVNV YNDTQIKLLI KQDDDLEVPA YEDIFRDEEE DEEHSGNDSD GSEPSEKRTR L DYKDDDIV EQTMRRRQRR EWEARRRDIL FDYEQYEYHG TSSAMVMFEL AWMLSKDLND MLWWAIVGLT DQWVQDKITQ MK YVTDVGV LQRHVSRHNH RNEDEENTLS VDCTRISFEY DLRLVLYQHW SLHDSLCNTS YTAARFKLWS VHGQKRLQEF LAD MGLPLK QVKQKFQAMD ISLKENLREM IEESANKFGM KDMRVQTFSI HFGFKHKFLA SDVVFATMSL MESPEKDGSG TDHF IQALD SLSRSNLDKL YHGLELAKKQ LRATQQTIAS CLCTNLVISQ GPFLYCSLME GTPDVMLFSR PASLSLLSKH LLKSF VCST KNRRCKLLPL VMAAPLSMEH GTVTVVGIPP ETDSSDRKNF FGRAFEKAAE STSSRMLHNH FDLSVIELKA EDRSKF LDA LISLLS

UniProtKB: Cell division control protein 45 homolog, Cell division control protein 45 homolog

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Macromolecule #10: DNA replication complex GINS protein PSF1

MacromoleculeName: DNA replication complex GINS protein PSF1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.022469 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL ...String:
MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRA LRWEYGSVLP NALRFHMAAE EMEWFNNYKR SLATYMRSLG GDEGLDITQD MKPPKSLYIE VRCLKDYGEF E VDDGTSVL LKKNSQHFLP RWKCEQLIRQ GVLEHILS

UniProtKB: DNA replication complex GINS protein PSF1

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Macromolecule #11: DNA replication complex GINS protein PSF2

MacromoleculeName: DNA replication complex GINS protein PSF2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.453713 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString:
MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI NLKQRQKCRL LPPEWMDVEK LEKMRDHERK EETFTPMPS PYYMELTKLL LNHASDNIPK ADEIRTLVKD MWDTRIAKLR VSADSFVRQQ EAHAKLDNLT LMEINTSGTF L TQALNHMY KLRTNLQPLE STQSQDF

UniProtKB: DNA replication complex GINS protein PSF2

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Macromolecule #12: DNA replication complex GINS protein PSF3

MacromoleculeName: DNA replication complex GINS protein PSF3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.562611 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL ...String:
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELP KIYQEGWRTV FSADPNVVDL HKMGPHFYGF GSQLLHFDSP ENADISQSLL QTFIGRFRRI MDSSQNAYNE D TSALVARL DEMERGLFQT GQKGLNDFQC WEKGQASQIT ASNLVQNYKK RKFTDMED

UniProtKB: DNA replication complex GINS protein PSF3

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Macromolecule #13: DNA replication complex GINS protein SLD5

MacromoleculeName: DNA replication complex GINS protein SLD5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.114236 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MWSHPQFEKG GGSGGGSGGS AWSHPQFEKS GLEVLFQGPM TEEVDFLGQD SDGGSEEVVL TPAELIERLE QAWMNEKFAP ELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS S LSPEELAF ...String:
MWSHPQFEKG GGSGGGSGGS AWSHPQFEKS GLEVLFQGPM TEEVDFLGQD SDGGSEEVVL TPAELIERLE QAWMNEKFAP ELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS S LSPEELAF AREFMANTES YLKNVALKHM PPNLQKVDLF RAVPKPDLDS YVFLRVRERQ ENILVEPDTD EQRDYVIDLE KG SQHLIRY KTIAPLVASG AVQLI

UniProtKB: DNA replication complex GINS protein SLD5

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Macromolecule #14: WD repeat and HMG-box DNA-binding protein 1

MacromoleculeName: WD repeat and HMG-box DNA-binding protein 1 / type: protein_or_peptide / ID: 14 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 130.098148 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MDYKDDDDKD YKDDDDKDYK DDDDKENLYF QGMPATRKPM RYGHTEGHTE VCFDDSGSFI VTCGSDGDVR IWEDLDDDDP KFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVPD GILTRFTTNA NHVVFNGDGT KIAAGSSDFL VKIVDVMDSS Q QKTFRGHD ...String:
MDYKDDDDKD YKDDDDKDYK DDDDKENLYF QGMPATRKPM RYGHTEGHTE VCFDDSGSFI VTCGSDGDVR IWEDLDDDDP KFINVGEKA YSCALKSGKL VTAVSNNTIQ VHTFPEGVPD GILTRFTTNA NHVVFNGDGT KIAAGSSDFL VKIVDVMDSS Q QKTFRGHD APVLSLSFDP KDIFLASASC DGSVRVWQIS DQTCAISWPL LQKCNDVINA KSICRLAWQP KSGKLLAIPV EK SVKLYRR ESWSHQFDLS DNFISQTLNI VTWSPCGQYL AAGSINGLII VWNVETKDCM ERVKHEKGYA ICGLAWHPTC GRI SYTDAE GNLGLLENVC DPSGKTSSSK VSSRVEKDYN DLFDGDDMSN AGDFLNDNAV EIPSFSKGII NDDEDDEDLM MASG RPRQR SHILEDDENS VDISMLKTGS SLLKEEEEDG QEGSIHNLPL VTSQRPFYDG PMPTPRQKPF QSGSTPLHLT HRFMV WNSI GIIRCYNDEQ DNAIDVEFHD TSIHHATHLS NTLNYTIADL SHEAILLACE STDELASKLH CLHFSSWDSS KEWIID LPQ NEDIEAICLG QGWAAAATSA LLLRLFTIGG VQKEVFSLAG PVVSMAGHGE QLFIVYHRGT GFDGDQCLGV QLLELGK KK KQILHGDPLP LTRKSYLAWI GFSAEGTPCY VDSEGIVRML NRGLGNTWTP ICNTREHCKG KSDHYWVVGI HENPQQLR C IPCKGSRFPP TLPRPAVAIL SFKLPYCQIA TEKGQMEEQF WRSVIFHNHL DYLAKNGYEY EESTKNQATK EQQELLMKM LALSCKLERE FRCVELADLM TQNAVNLAIK YASRSRKLIL AQKLSELAVE KAAELTATQV EEEEEEEDFR KKLNAGYSNT ATEWSQPRF RNQVEEDAED SGEADDEEKP EIHKPGQNSF SKSTNSSDVS AKSGAVTFSS QGRVNPFKVS ASSKEPAMSM N SARSTNIL DNMGKSSKKS TALSRTTNNE KSPIIKPLIP KPKPKQASAA SYFQKRNSQT NKTEEVKEEN LKNVLSETPA IC PPQNTEN QRPKTGFQMW LEENRSNILS DNPDFSDEAD IIKEGMIRFR VLSTEERKVW ANKAKGETAS EGTEAKKRKR VVD ESDETE NQEEKAKENL NLSKKQKPLD FSTNQKLSAF AFKQE

UniProtKB: WD repeat and HMG-box DNA-binding protein 1

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Macromolecule #15: Protein timeless homolog

MacromoleculeName: Protein timeless homolog / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 138.903031 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: GMDLHMMNCE LLATCSALGY LEGDTYHKEP DCLESVKDLI RYLRHEDETR DVRQQLGAAQ ILQSDLLPIL TQHHQDKPLF DAVIRLMVN LTQPALLCFG NLPKEPSFRH HFLQVLTYLQ AYKEAFASEK AFGVLSETLY ELLQLGWEER QEEDNLLIER I LLLVRNIL ...String:
GMDLHMMNCE LLATCSALGY LEGDTYHKEP DCLESVKDLI RYLRHEDETR DVRQQLGAAQ ILQSDLLPIL TQHHQDKPLF DAVIRLMVN LTQPALLCFG NLPKEPSFRH HFLQVLTYLQ AYKEAFASEK AFGVLSETLY ELLQLGWEER QEEDNLLIER I LLLVRNIL HVPADLDQEK KIDDDASAHD QLLWAIHLSG LDDLLLFLAS SSAEEQWSLH VLEIVSLMFR DQNPEQLAGV GQ GRLAQER SADFAELEVL RQREMAEKKT RALQRGNRHS RFGGSYIVQG LKSIGERDLI FHKGLHNLRN YSSDLGKQPK KVP KRRQAA RELSIQRRSA LNVRLFLRDF CSEFLENCYN RLMGSVKDHL LREKAQQHDE TYYMWALAFF MAFNRAASFR PGLV SETLS VRTFHFIEQN LTNYYEMMLT DRKEAASWAR RMHLALKAYQ ELLATVNEMD ISPDEAVRES SRIIKNNIFY VMEYR ELFL ALFRKFDERC QPRSFLRDLV ETTHLFLKML ERFCRSRGNL VVQNKQKKRR KKKKKVLDQA IVSGNVPSSP EEVEAV WPA LAEQLQCCAQ NSELSMDSVV PFDAASEVPV EEQRAEAMVR IQDCLLAGQA PQALTLLRSA REVWPEGDVF GSQDISP EE EIQLLKQILS APLPRQQGPE ERGAEEEEEE EEEEEEELQV VQVSEKEFNF LDYLKRFACS TVVRAYVLLL RSYQQNSA H TNHCIVKMLH RLAHDLKMEA LLFQLSVFCL FNRLLSDPAA GAYKELVTFA KYILGKFFAL AAVNQKAFVE LLFWKNTAV VREMTEGYGS LDDRSSSRRA PTWSPEEEAH LRELYLANKD VEGQDVVEAI LAHLNTVPRT RKQIIHHLVQ MGLADSVKDF QRKGTHIVL WTGDQELELQ RLFEEFRDSD DVLGHIMKNI TAKRSRARIV DKLLALGLVA ERRELYKKRQ KKLASSILPN G AESLKDFC QEDLEEEENL PEEDSEEEEE GGSEAEQVQG SLVLSNENLG QSLHQEGFSI PLLWLQNCLI RAADDREEDG CS QAVPLVP LTEENEEAME NEQFQQLLRK LGVRPPASGQ ETFWRIPAKL SPTQLRRAAA SLSQPEEEQK LQPELQPKVP GEQ GSDEEH CKEHRAQALR ALLLAHKKKA GLASPEEEDA VGKEPLKAAP KKRQLLDSDE EQEEDEGRNR APELGAPGIQ KKKR YQIED DEDD

UniProtKB: Protein timeless homolog

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Macromolecule #16: TIMELESS-interacting protein

MacromoleculeName: TIMELESS-interacting protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.600223 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD ...String:
MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAK FKGKGHEAED LKMLIRHMEH WAHRLFPKLQ FEDFIDRVEY LGSKKEVQTC LKRIRLDLPI LHEDFVSNND E VAENNEHD VTSTELDPFL TNLSESEMFA SELSRSLTEE QQQRIERNKQ LALERRQAKL LSNSQTLGND MLMNTPRAHT VE EVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR

UniProtKB: TIMELESS-interacting protein

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Macromolecule #19: Claspin

MacromoleculeName: Claspin / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 155.184703 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK ...String:
MTGEVGSEVH LEINDPNVIS QEEADSPSDS GQGSYETIGP LSEGDSDEEI FVSKKLKNRK VLQDSDSETE DTNASPEKTT YDSAEEENK ENLYAGKNTK IKRIYKTVAD SDESYMEKSL YQENLEAQVK PCLELSLQSG NSTDFTTDRK SSKKHIHDKE G TAGKAKVK SKRRLEKEER KMEKIRQLKK KETKNQEDDV EQPFNDSGCL LVDKDLFETG LEDENNSPLE DEESLESIRA AV KNKVKKH KKKEPSLESG VHSFEEGSEL SKGTTRKERK AARLSKEALK QLHSETQRLI RESALNLPYH MPENKTIHDF FKR KPRPTC HGNAMALLKS SKYQSSHHKE IIDTANTTEM NSDHHSKGSE QTTGAENEVE TNALPVVSKE TQIITGSDES CRKD LVKNE ELEIQEKQKQ SDIRPSPGDS SVLQQESNFL GNNHSEECQV GGLVAFEPHA LEGEGPQNPE ETDEKVEEPE QQNKS SAVG PPEKVRRFTL DRLKQLGVDV SIKPRLGADE DSFVILEPET NRELEALKQR FWKHANPAAK PRAGQTVNVN VIVKDM GTD GKEELKADVV PVTLAPKKLD GASHTKPGEK LQVLKAKLQE AMKLRRFEER QKRQALFKLD NEDGFEEEEE EEEEMTD ES EEDGEEKVEK EEKEEELEEE EEKEEEEEEE GNQETAEFLL SSEEIETKDE KEMDKENNDG SSEIGKAVGF LSVPKSLS S DSTLLLFKDS SSKMGYFPTE EKSETDENSG KQPSKLDEDD SCSLLTKESS HNSSFELIGS TIPSYQPCNR QTGRGTSFF PTAGGFRSPS PGLFRASLVS SASKSSGKLS EPSLPIEDSQ DLYNASPEPK TLFLGAGDFQ FCLEDDTQSQ LLDADGFLNV RNHRNQYQA LKPRLPLASM DENAMDANMD ELLDLCTGKF TSQAEKHLPR KSDKKENMEE LLNLCSGKFT SQDASTPASS E LNKQEKES SMGDPMEEAL ALCSGSFPTD KEEEDEEEEF GDFRLVSNDN EFDSDEDEHS DSGNDLALED HEDDDEEELL KR SEKLKRQ MRLRKYLEDE AEVSGSDVGS EDEYDGEEID EYEEDVIDEV LPSDEELQSQ IKKIHMKTML DDDKRQLRLY QER YLADGD LHSDGPGRMR KFRWKNIDDA SQMDLFHRDS DDDQTEEQLD ESEARWRKER IEREQWLRDM AQQGKITAEE EEEI GEDSQ FMILAKKVTA KALQKNASRP MVIQESKSLL RNPFEAIRPG SAQQVKTGSL LNQPKAVLQK LAALSDHNPS APRNS RNFV FHTLSPVKAE AAKESSKSQV KKRGPSFMTS PSPKHLKTDD STSGLTRSIF KYLESLEVLF QGPDYKDDDD KDYKDD DDK DYKDDDDK

UniProtKB: Claspin

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Macromolecule #17: Leading strand DNA

MacromoleculeName: Leading strand DNA / type: dna / ID: 17 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.396836 KDa
SequenceString: (DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DG)(DG)(DT)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG) (DA)(DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG) (DA) (DG)(DT)(DG)(DT)(DG)(DT) ...String:
(DT)(DA)(DG)(DA)(DG)(DT)(DA)(DG)(DG)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DG)(DG)(DT)(DA) (DA)(DG)(DT)(DG)(DA)(DT)(DT)(DA)(DG) (DA)(DG)(DA)(DA)(DT)(DT)(DG)(DG)(DA)(DG) (DA) (DG)(DT)(DG)(DT)(DG)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)

+
Macromolecule #18: Lagging Strand DNA

MacromoleculeName: Lagging Strand DNA / type: dna / ID: 18 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.407533 KDa
SequenceString: (DG)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DG)(DG) (DC)(DA)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA) (DC)(DT)(DC)(DT)(DC)(DC)(DA)(DA)(DT) (DT)(DC)(DT)(DC)(DT)(DA)(DA)(DT)(DC)(DA) (DC) (DT)(DT)(DA)(DC)(DC)(DA) ...String:
(DG)(DG)(DC)(DA)(DG)(DG)(DC)(DA)(DG)(DG) (DC)(DA)(DG)(DG)(DC)(DA)(DC)(DA)(DC)(DA) (DC)(DT)(DC)(DT)(DC)(DC)(DA)(DA)(DT) (DT)(DC)(DT)(DC)(DT)(DA)(DA)(DT)(DC)(DA) (DC) (DT)(DT)(DA)(DC)(DC)(DA)(DT)(DC) (DA)(DC)(DT)(DT)(DC)(DC)

+
Macromolecule #20: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 20 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #21: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 21 / Number of copies: 3 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #22: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 22 / Number of copies: 3 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

+
Macromolecule #23: SULFATE ION

MacromoleculeName: SULFATE ION / type: ligand / ID: 23 / Number of copies: 1 / Formula: SO4
Molecular weightTheoretical: 96.063 Da
Chemical component information

ChemComp-SO4:
SULFATE ION

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 39.8 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.1 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 490000
Startup modelType of model: INSILICO MODEL
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 110000
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-7pfo:
Core human replisome

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