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- EMDB-9624: The Cryo-EM Structure of Human Pre-catalytic Spliceosome (B compl... -

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Entry
Database: EMDB / ID: 9624
TitleThe Cryo-EM Structure of Human Pre-catalytic Spliceosome (B complex) at 3.8 angstrom resolution
Map dataThe 3.8 Angstrom map of the human B complex
SampleHuman Pre-catalytic spliceosome (B complex)
  • Pre-mRNA-processing-splicing factor 8
  • (nucleic-acidNucleic acid) x 5
  • Thioredoxin-like protein 4A
  • 116 kDa U5 small nuclear ribonucleoprotein component
  • Pre-mRNA-processing factor 6
  • NHP2-like protein 1
  • (U4/U6 small nuclear ribonucleoprotein ...) x 3
  • U4/U6.U5 tri-snRNP-associated protein 1
  • SmB
  • (Small nuclear ribonucleoprotein Sm ...) x 3
  • SmE
  • SmF
  • Small nuclear ribonucleoprotein G
  • U5 small nuclear ribonucleoprotein 40 kDa protein
  • WW domain-binding protein 4
  • Peptidyl-prolyl cis-trans isomerase H
  • Ubiquitin-like protein 5
  • Microfibrillar-associated protein 1
  • Pre-mRNA-splicing factor 38A
  • Zinc finger matrin-type protein 2
  • WD40 repeat-containing protein SMU1
  • (U2 small nuclear ribonucleoprotein ...) x 2
  • (Splicing factor 3A subunit ...) x 3
  • (U6 snRNA-associated Sm-like protein ...) x 7
  • (Splicing factor 3B subunit ...) x 2
  • SF3b14a, Splicing factor 3B subunit 6
  • PHD finger-like domain-containing protein 5A
  • SF3b5, Splicing factor 3B subunit 5
  • SF3b145, Splicing factor 3B subunit 2
  • SF3b49, Splicing factor 3B subunit 4
  • Brr2, U5 small nuclear ribonucleoprotein 200 kDa helicase
  • (ligand) x 3
Function / homologyU1-C, C2H2-type zinc finger / Splicing factor 3B, subunit 5 / RNA recognition motif, spliceosomal PrP8 / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding / Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding / Prp31 C-terminal / Ribosomal protein L7Ae/L8/Nhp2 family / WD40-repeat-containing domain / Sm-like protein Lsm7 / U6 snRNA-associated Sm-like protein LSm2 ...U1-C, C2H2-type zinc finger / Splicing factor 3B, subunit 5 / RNA recognition motif, spliceosomal PrP8 / Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding / Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding / Prp31 C-terminal / Ribosomal protein L7Ae/L8/Nhp2 family / WD40-repeat-containing domain / Sm-like protein Lsm7 / U6 snRNA-associated Sm-like protein LSm2 / WD40 repeat, conserved site / Sm-like protein Lsm6/SmF / Armadillo-type fold / WD40/YVTN repeat-like-containing domain superfamily / Splicing factor 3B subunit 1 / Pre-mRNA processing factor 4 (PRP4)-like / Immunoglobulin E-set / Ribosomal protein S5 domain 2-type fold, subgroup / Tetratricopeptide repeat / G-protein beta WD-40 repeat / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp31 / Cyclophilin-like domain superfamily / Pre-mRNA-processing-splicing factor 8 / P-loop containing nucleoside triphosphate hydrolase / Small nuclear ribonucleoprotein Sm D2 / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Pre-mRNA-processing factor 6/Prp1/STA1 / U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6 small nuclear ribonucleoprotein Prp3 / Ribosomal protein S5 domain 2-type fold / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / Pre-mRNA-splicing factor 38, C-terminal / Domain of unknown function DUF3449 / Zinc finger, double-stranded RNA binding / Splicing factor 3A subunit 1 / PRP8 domain IV core / Splicing factor SF3a60 binding domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site / Helicase superfamily 1/2, ATP-binding domain / Tetratricopeptide repeat-containing domain / 50S ribosomal protein L30e-like / Ribosomal protein L7Ae conserved site / Pre-mRNA-splicing factor 38 / SNU66/SART1 family / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / Sec63 domain / Translation elongation factor EFTu-like, domain 2 / Dim1 family / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Matrin/U1-C-like, C2H2-type zinc finger / PHF5-like / U2A'/phosphoprotein 32 family A, C-terminal / HAT (Half-A-TPR) repeat / SAP domain / Nop domain / PWI domain / H/ACA ribonucleoprotein complex, subunit Nhp2, eukaryote / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Small GTP-binding protein domain / Translation elongation factor EFG/EF2, domain IV / PROCT domain / DEAD/DEAH box helicase domain / NOSIC / Nucleotide-binding alpha-beta plait domain superfamily / PROCN domain / PRO8NT domain / Ribonuclease H-like superfamily / Tetratricopeptide-like helical domain superfamily / Armadillo-like helical / LSM domain superfamily / PSP, proline-rich / Domain of unknown function DUF1115 / PRP1 splicing factor, N-terminal / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Micro-fibrillar-associated protein 1, C-terminal / Translation protein, beta-barrel domain superfamily / Domain of unknown function DUF382 / CTLH, C-terminal LisH motif / LIS1 homology motif / Helix hairpin bin domain superfamily / Ubiquitin-like domain superfamily / Helicase, C-terminal / PWI domain / CPSF A subunit region / Elongation factor Tu domain 2 / Mitosis protein DIM1 / Sec63 Brl domain / SAP domain / Surp module / snoRNA binding domain, fibrillarin / LSM domain / PRP38 family / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / Elongation factor G C-terminus / WD domain, G-beta repeat / WW domain / Helicase conserved C-terminal domain / DEAD/DEAH box helicase
Function and homology information
SourceHomo sapiens (human) / Human (human)
Methodsingle particle reconstruction / cryo EM / 3.8 Å resolution
AuthorsZhan X / Yan C / Zhang X / Shi Y
CitationJournal: Cell Res. / Year: 2018
Title: Structures of the human pre-catalytic spliceosome and its precursor spliceosome.
Authors: Xiechao Zhan / Chuangye Yan / Xiaofeng Zhang / Jianlin Lei / Yigong Shi
Validation ReportPDB-ID: 6ahd

SummaryFull reportAbout validation report
DateDeposition: Aug 17, 2018 / Header (metadata) release: Nov 14, 2018 / Map release: Nov 14, 2018 / Last update: Dec 19, 2018

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.035
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.035
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-6ahd
  • Surface level: 0.035
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_9624.map.gz (map file in CCP4 format, 256001 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
400 pix
1.34 Å/pix.
= 535.2 Å
400 pix
1.34 Å/pix.
= 535.2 Å
400 pix
1.34 Å/pix.
= 535.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.338 Å
Density
Contour Level:0.03 (by author), 0.035 (movie #1):
Minimum - Maximum-0.12918533 - 0.25120035
Average (Standard dev.)0.00051532657 (0.0070919623)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions400400400
Origin0.00.00.0
Limit399.0399.0399.0
Spacing400400400
CellA=B=C: 535.2 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.3381.3381.338
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z535.200535.200535.200
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.1290.2510.001

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Supplemental data

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Sample components

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Entire Human Pre-catalytic spliceosome (B complex)

EntireName: Human Pre-catalytic spliceosome (B complex) / Number of components: 53

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Component #1: protein, Human Pre-catalytic spliceosome (B complex)

ProteinName: Human Pre-catalytic spliceosome (B complex) / Recombinant expression: No
SourceSpecies: Homo sapiens (human)

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Component #2: protein, Pre-mRNA-processing-splicing factor 8

ProteinName: Pre-mRNA-processing-splicing factor 8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 273.97425 kDa
SourceSpecies: Human (human)

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Component #3: nucleic-acid, U4snRNA

Nucleic-acidName: U4snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AGCUUUGCGC AGUGGCAGUA UCGUAGCCAA UGAGGUCUAU CCGAGGCGCG AUUAUUGCUA AUUGAAAACU UUUCCCAAUA CCCCGCCGUG ACGACUUGCA AUAUAGUCGG CACUGGCAAU UUUUGACAGU CUCUACGGAG ACUG
MassTheoretical: 46.181289 kDa
SourceSpecies: Human (human)

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Component #4: nucleic-acid, U5snRNA

Nucleic-acidName: U5snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAUU UUUUGAGGCC UUGCUUUGGC AAGGCUA
MassTheoretical: 37.254855 kDa
SourceSpecies: Human (human)

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Component #5: nucleic-acid, U6snRNA

Nucleic-acidName: U6snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGUG AAGCGUUCCA UAUUUUU
MassTheoretical: 34.404438 kDa
SourceSpecies: Human (human)

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Component #6: nucleic-acid, pre-mRNA

Nucleic-acidName: pre-mRNAPrimary transcript / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GGGAAUACAC GGAAUUCGAG CUCGCCCACU CUUGGAUCGG AAACCCGUCG GCCUCCGAAC GGUAAGAGCC UAGCAUGUAG AACUGGUUAC CUGCAGCCCA AGCUUGCGUA CACCAUCAGG GUACGUACUA GUACGUACAC CAUCAGGGUA CGGCUGCACG UCUAGGGCGC AGUAGUCCAG GGUUUCCUUG AUGAUGUCAU ACUUAUCCUG UCCCUUUUUU UUCCACGGCU CGCGGUUGAG GACAAACUCU UCGCGGUCUU UCCAGUGGGG AUCC
MassTheoretical: 87.892727 kDa
SourceSpecies: Human (human)

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Component #7: protein, Thioredoxin-like protein 4A

ProteinName: Thioredoxin-like protein 4A / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.807346 kDa
SourceSpecies: Human (human)

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Component #8: protein, 116 kDa U5 small nuclear ribonucleoprotein component

ProteinName: 116 kDa U5 small nuclear ribonucleoprotein component / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 109.560625 kDa
SourceSpecies: Human (human)

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Component #9: protein, Pre-mRNA-processing factor 6

ProteinName: Pre-mRNA-processing factor 6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 107.092242 kDa
SourceSpecies: Human (human)

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Component #10: protein, NHP2-like protein 1

ProteinName: NHP2-like protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.191524 kDa
SourceSpecies: Human (human)

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Component #11: protein, U4/U6 small nuclear ribonucleoprotein Prp31

ProteinName: U4/U6 small nuclear ribonucleoprotein Prp31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 55.528969 kDa
SourceSpecies: Human (human)

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Component #12: protein, U4/U6.U5 tri-snRNP-associated protein 1

ProteinName: U4/U6.U5 tri-snRNP-associated protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 90.414117 kDa
SourceSpecies: Human (human)

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Component #13: protein, U4/U6 small nuclear ribonucleoprotein Prp3

ProteinName: U4/U6 small nuclear ribonucleoprotein Prp3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 77.669188 kDa
SourceSpecies: Human (human)

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Component #14: protein, SmB

ProteinName: SmB / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 23.686004 kDa
SourceSpecies: Human (human)

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Component #15: protein, Small nuclear ribonucleoprotein Sm D1

ProteinName: Small nuclear ribonucleoprotein Sm D1 / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 13.310653 kDa
SourceSpecies: Human (human)

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Component #16: protein, Small nuclear ribonucleoprotein Sm D2

ProteinName: Small nuclear ribonucleoprotein Sm D2 / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 13.551928 kDa
SourceSpecies: Human (human)

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Component #17: protein, SmE

ProteinName: SmE / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 9.734171 kDa
SourceSpecies: Human (human)

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Component #18: protein, SmF

ProteinName: SmF / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 10.817601 kDa
SourceSpecies: Human (human)

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Component #19: protein, Small nuclear ribonucleoprotein G

ProteinName: Small nuclear ribonucleoprotein G / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 8.508084 kDa
SourceSpecies: Human (human)

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Component #20: protein, Small nuclear ribonucleoprotein Sm D3

ProteinName: Small nuclear ribonucleoprotein Sm D3 / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 13.940308 kDa
SourceSpecies: Human (human)

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Component #21: protein, U5 small nuclear ribonucleoprotein 40 kDa protein

ProteinName: U5 small nuclear ribonucleoprotein 40 kDa protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.359492 kDa
SourceSpecies: Human (human)

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Component #22: protein, WW domain-binding protein 4

ProteinName: WW domain-binding protein 4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 42.575801 kDa
SourceSpecies: Human (human)

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Component #23: protein, Peptidyl-prolyl cis-trans isomerase H

ProteinName: Peptidyl-prolyl cis-trans isomerase H / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.230117 kDa
SourceSpecies: Human (human)

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Component #24: protein, Ubiquitin-like protein 5

ProteinName: Ubiquitin-like protein 5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.560945 kDa
SourceSpecies: Human (human)

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Component #25: protein, Microfibrillar-associated protein 1

ProteinName: Microfibrillar-associated protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 52.050527 kDa
SourceSpecies: Human (human)

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Component #26: protein, Pre-mRNA-splicing factor 38A

ProteinName: Pre-mRNA-splicing factor 38A / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 37.563863 kDa
SourceSpecies: Human (human)

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Component #27: protein, Zinc finger matrin-type protein 2

ProteinName: Zinc finger matrin-type protein 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.664047 kDa
SourceSpecies: Human (human)

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Component #28: protein, WD40 repeat-containing protein SMU1

ProteinName: WD40 repeat-containing protein SMU1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 57.616742 kDa
SourceSpecies: Human (human)

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Component #29: nucleic-acid, U2snRNA

Nucleic-acidName: U2snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAUA UUAAAUGGAU UUUUGGAGCA GGGAGAUGGA AUAGGAGCUU GCUCCGUCCA CUCCACGCAU CGACCUGGUA UUGCAGUACC UCCAGGAACG GUGCACCC
MassTheoretical: 60.186445 kDa
SourceSpecies: Human (human)

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Component #30: protein, U2 small nuclear ribonucleoprotein A'

ProteinName: U2 small nuclear ribonucleoprotein A' / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.456584 kDa
SourceSpecies: Human (human)

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Component #31: protein, U2 small nuclear ribonucleoprotein B''

ProteinName: U2 small nuclear ribonucleoprotein B'' / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.524367 kDa
SourceSpecies: Human (human)

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Component #32: protein, Splicing factor 3A subunit 1

ProteinName: Splicing factor 3A subunit 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 88.991094 kDa
SourceSpecies: Human (human)

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Component #33: protein, Splicing factor 3A subunit 2

ProteinName: Splicing factor 3A subunit 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 49.327355 kDa
SourceSpecies: Human (human)

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Component #34: protein, Splicing factor 3A subunit 3

ProteinName: Splicing factor 3A subunit 3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 58.934844 kDa
SourceSpecies: Human (human)

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Component #35: protein, U6 snRNA-associated Sm-like protein LSm2

ProteinName: U6 snRNA-associated Sm-like protein LSm2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.847495 kDa
SourceSpecies: Human (human)

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Component #36: protein, U6 snRNA-associated Sm-like protein LSm3

ProteinName: U6 snRNA-associated Sm-like protein LSm3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.85939 kDa
SourceSpecies: Human (human)

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Component #37: protein, U6 snRNA-associated Sm-like protein LSm4

ProteinName: U6 snRNA-associated Sm-like protein LSm4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.375775 kDa
SourceSpecies: Human (human)

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Component #38: protein, U6 snRNA-associated Sm-like protein LSm5

ProteinName: U6 snRNA-associated Sm-like protein LSm5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.945448 kDa
SourceSpecies: Human (human)

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Component #39: protein, U6 snRNA-associated Sm-like protein LSm6

ProteinName: U6 snRNA-associated Sm-like protein LSm6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.139571 kDa
SourceSpecies: Human (human)

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Component #40: protein, U6 snRNA-associated Sm-like protein LSm7

ProteinName: U6 snRNA-associated Sm-like protein LSm7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.617363 kDa
SourceSpecies: Human (human)

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Component #41: protein, U6 snRNA-associated Sm-like protein LSm8

ProteinName: U6 snRNA-associated Sm-like protein LSm8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.410589 kDa
SourceSpecies: Human (human)

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Component #42: protein, U4/U6 small nuclear ribonucleoprotein Prp4

ProteinName: U4/U6 small nuclear ribonucleoprotein Prp4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 58.536105 kDa
SourceSpecies: Human (human)

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Component #43: protein, Splicing factor 3B subunit 1

ProteinName: Splicing factor 3B subunit 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 146.024938 kDa
SourceSpecies: Human (human)

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Component #44: protein, Splicing factor 3B subunit 3

ProteinName: Splicing factor 3B subunit 3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 135.718844 kDa
SourceSpecies: Human (human)

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Component #45: protein, SF3b14a, Splicing factor 3B subunit 6

ProteinName: SF3b14a, Splicing factor 3B subunit 6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.6069 kDa
SourceSpecies: Human (human)

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Component #46: protein, PHD finger-like domain-containing protein 5A

ProteinName: PHD finger-like domain-containing protein 5A / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.427524 kDa
SourceSpecies: Human (human)

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Component #47: protein, SF3b5, Splicing factor 3B subunit 5

ProteinName: SF3b5, Splicing factor 3B subunit 5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.149369 kDa
SourceSpecies: Human (human)

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Component #48: protein, SF3b145, Splicing factor 3B subunit 2

ProteinName: SF3b145, Splicing factor 3B subunit 2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 100.377812 kDa
SourceSpecies: Human (human)

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Component #49: protein, SF3b49, Splicing factor 3B subunit 4

ProteinName: SF3b49, Splicing factor 3B subunit 4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 44.43657 kDa
SourceSpecies: Human (human)

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Component #50: protein, Brr2, U5 small nuclear ribonucleoprotein 200 kDa helicase

ProteinName: Brr2, U5 small nuclear ribonucleoprotein 200 kDa helicase
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 244.823422 kDa
SourceSpecies: Human (human)

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Component #51: ligand, INOSITOL HEXAKISPHOSPHATE

LigandName: INOSITOL HEXAKISPHOSPHATEPhytic acid / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.660035 kDa
SourceSpecies: Human (human)

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Component #52: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa
SourceSpecies: Human (human)

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Component #53: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.430505 MDa
SourceSpecies: Human (human)

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionpH: 7.9
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 45 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 137853
3D reconstructionResolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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