|Entry||Database: EMDB / ID: 1212|
|Title||Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit.|
|Map data||map of 40S ribosome|
|Source||Saccharomyces cerevisiae (baker's yeast)|
|Method||single particle reconstruction / cryo EM / 32 Å resolution|
|Authors||Schafer T / Maco B / Petfalski E / Tollervey D / Bottcher B / Aebi U / Hurt E|
|Citation||Journal: Nature / Year: 2006|
Title: Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit.
Authors: Thorsten Schäfer / Bohumil Maco / Elisabeth Petfalski / David Tollervey / Bettina Böttcher / Ueli Aebi / Ed Hurt
|Date||Deposition: Mar 29, 2006 / Header (metadata) release: Apr 3, 2006 / Map release: Jun 1, 2006 / Last update: Oct 17, 2012|
|Structure viewer||EM map: |
Downloads & links
|File||emd_1212.map.gz (map file in CCP4 format, 2001 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 5.72 Å|
CCP4 map header:
-Entire 40S Ribosome
|Entire||Name: 40S Ribosome / Details: sample was purified from S. cervisiae / Oligomeric State: monomer / Number of components: 1|
-Component #1: ribosome-eukaryote, 40S-ribosome
|Ribosome-eukaryote||Name: 40S-ribosome / Eukaryote: SSU 40S / Recombinant expression: No|
|Source||Species: Saccharomyces cerevisiae (baker's yeast)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||Buffer solution: 50mM Tris-HCl, 100mM NaCl, 10mM MgCl2, 0.5mM DTT|
|Support film||400 mesh copper rhodium grids (Maxtaform)|
|Vitrification||Instrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Temperature: 295 K / Humidity: 90 % / Method: Blot for 15 s with Whatman No 1|
Details: Vitrification instrument: Plunger with environmental chamber
-Electron microscopy imaging
|Imaging||Microscope: FEI/PHILIPS CM200FEG|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 50000 X (nominal) / Astigmatism: bjective lens astigmatism was corrected at / Cs: 2 mm / Imaging mode: BRIGHT FIELD / Defocus: 3200 - 5000 nm|
|Specimen Holder||Holder: Side entry liquid nitrogen-cooled cryo specimen holder|
Model: GATAN LIQUID NITROGEN / Temperature: 94 K
|Camera||Detector: GENERIC CCD|
|Image acquisition||Number of digital images: 69 / Sampling size: 14 microns / Bit depth: 12|
|Processing||Method: single particle reconstruction / Number of class averages: 236 / Number of projections: 4827|
Details: 236 out of 400 classes were used for final reconstruction
Applied symmetry: C1 (asymmetric)
|3D reconstruction||Algorithm: weighted back projection / Software: IMAGIC 5 / CTF correction: Each Particle / Resolution: 32 Å / Resolution method: FSC 0.5 / Euler angles: IMAGIC|
Details: reconstructions were calculated from half of the class averages. Euler angles of class averages were determined by projection matching
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