+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-11439 | |||||||||
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タイトル | Structure of a yeast ABCE1-bound 48S initiation complex | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | Translation / Initiation / Ribosome Recycling / ABC Proteins / RIBOSOME | |||||||||
機能・相同性 | 機能・相同性情報 formation of translation initiation ternary complex / eukaryotic initiation factor eIF2 binding / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / incipient cellular bud site / translation reinitiation / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / multi-eIF complex / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) ...formation of translation initiation ternary complex / eukaryotic initiation factor eIF2 binding / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / incipient cellular bud site / translation reinitiation / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / multi-eIF complex / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / cytoplasmic translational initiation / eukaryotic 43S preinitiation complex / formation of translation preinitiation complex / ribosome disassembly / eukaryotic 48S preinitiation complex / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / RMTs methylate histone arginines / positive regulation of translational fidelity / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / regulation of translational initiation / nonfunctional rRNA decay / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / preribosome, small subunit precursor / Ribosomal scanning and start codon recognition / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / regulation of amino acid metabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal small subunit binding / 90S preribosome / G-protein alpha-subunit binding / positive regulation of protein kinase activity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / Ub-specific processing proteases / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / translation regulator activity / translational termination / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / negative regulation of translational initiation / DNA-(apurinic or apyrimidinic site) endonuclease activity / translation initiation factor binding / translation initiation factor activity / cellular response to amino acid starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / GTPase activator activity / rescue of stalled ribosome / ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / cytosolic ribosome assembly / positive regulation of translation / small-subunit processome / translational initiation / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / cytoplasmic stress granule / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / double-stranded RNA binding / small ribosomal subunit rRNA binding / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / iron ion binding / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / translation / negative regulation of gene expression / mRNA binding / ubiquitin protein ligase binding / GTP binding / nucleolus / protein kinase binding / ATP hydrolysis activity 類似検索 - 分子機能 | |||||||||
生物種 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 6.2 Å | |||||||||
データ登録者 | Kratzat H / Mackens-Kiani T | |||||||||
資金援助 | ドイツ, フランス, 2件
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引用 | ジャーナル: EMBO J / 年: 2021 タイトル: A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes. 著者: Hanna Kratzat / Timur Mackens-Kiani / Michael Ameismeier / Mia Potocnjak / Jingdong Cheng / Estelle Dacheux / Abdelkader Namane / Otto Berninghausen / Franz Herzog / Micheline Fromont-Racine ...著者: Hanna Kratzat / Timur Mackens-Kiani / Michael Ameismeier / Mia Potocnjak / Jingdong Cheng / Estelle Dacheux / Abdelkader Namane / Otto Berninghausen / Franz Herzog / Micheline Fromont-Racine / Thomas Becker / Roland Beckmann / 要旨: In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. ...In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. These comprise/include the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of the next 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S, but also with later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide-binding domains, while interacting with the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus providing a structural explanation for its antagonistic role with respect to mRNA binding. Overall, the native human samples provide a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and the eIF2 ternary complex containing the initiator tRNA. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_11439.map.gz | 140.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-11439-v30.xml emd-11439.xml | 66 KB 66 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_11439_fsc.xml | 12.9 KB | 表示 | FSCデータファイル |
画像 | emd_11439.png | 58.1 KB | ||
Filedesc metadata | emd-11439.cif.gz | 14.5 KB | ||
その他 | emd_11439_half_map_1.map.gz emd_11439_half_map_2.map.gz | 141.1 MB 140.8 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-11439 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11439 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_11439_validation.pdf.gz | 1012.5 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_11439_full_validation.pdf.gz | 1012.1 KB | 表示 | |
XML形式データ | emd_11439_validation.xml.gz | 20.4 KB | 表示 | |
CIF形式データ | emd_11439_validation.cif.gz | 26.7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11439 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11439 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_11439.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.084 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-ハーフマップ: #2
ファイル | emd_11439_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_11439_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : ABCE1-bound 48S initiation complex
+超分子 #1: ABCE1-bound 48S initiation complex
+分子 #1: 18S ribosomal RNA
+分子 #37: tRNA
+分子 #42: mRNA
+分子 #2: 40S ribosomal protein S0-A
+分子 #3: 40S ribosomal protein S1-A
+分子 #4: 40S ribosomal protein S2
+分子 #5: 40S ribosomal protein S4-A
+分子 #6: 40S ribosomal protein S6-A
+分子 #7: 40S ribosomal protein S7-A
+分子 #8: 40S ribosomal protein S8-A
+分子 #9: 40S ribosomal protein S9-A
+分子 #10: 40S ribosomal protein S11-A
+分子 #11: 40S ribosomal protein S13
+分子 #12: 40S ribosomal protein S14-A
+分子 #13: 40S ribosomal protein S21-A
+分子 #14: 40S ribosomal protein S22-A
+分子 #15: 40S ribosomal protein S23-A
+分子 #16: 40S ribosomal protein S24-A
+分子 #17: 40S ribosomal protein S26-A
+分子 #18: 40S ribosomal protein S27-A
+分子 #19: 40S ribosomal protein S30-A
+分子 #20: 40S ribosomal protein S15
+分子 #21: 40S ribosomal protein S3
+分子 #22: Rps5p
+分子 #23: 40S ribosomal protein S10-A
+分子 #24: 40S ribosomal protein S12
+分子 #25: 40S ribosomal protein S16-A
+分子 #26: 40S ribosomal protein S17-A
+分子 #27: 40S ribosomal protein S18-A
+分子 #28: 40S ribosomal protein S19-A
+分子 #29: 40S ribosomal protein S20
+分子 #30: 40S ribosomal protein S25-A
+分子 #31: 40S ribosomal protein S29-A
+分子 #32: Ubiquitin-40S ribosomal protein S31
+分子 #33: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #34: 40S ribosomal protein S28-B
+分子 #35: Eukaryotic translation initiation factor 3 subunit I
+分子 #36: Eukaryotic translation initiation factor 3 subunit G
+分子 #38: Eukaryotic translation initiation factor 5
+分子 #39: Eukaryotic translation initiation factor 3 subunit A
+分子 #40: Eukaryotic translation initiation factor 3 subunit B
+分子 #41: Eukaryotic translation initiation factor 3 subunit C
+分子 #43: Eukaryotic translation initiation factor 1A
+分子 #44: Translation initiation factor RLI1
+分子 #45: MAGNESIUM ION
+分子 #46: ZINC ION
+分子 #47: ADENOSINE-5'-DIPHOSPHATE
+分子 #48: ADENOSINE-5'-TRIPHOSPHATE
+分子 #49: IRON/SULFUR CLUSTER
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 2.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |