EMDB-11458: Structure of a human ABCE1-bound 43S pre-initiation complex - State II

Basic information

• Entry: Database: EMDB / ID: EMD-11458
• Title: Structure of a human ABCE1-bound 43S pre-initiation complex - State II

Sample

• Complex: ABCE1-bound 43S initiation complex
  • Protein or peptide: x 49 types
  • RNA: x 1 types
• Ligand: x 5 types

• Keywords: Translation / Initiation / Ribosome Recycling / ABC Proteins / RIBOSOME

Function / homology

Function:

negative regulation of endoribonuclease activity / CTPase activity / positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / translation reinitiation / eukaryotic translation initiation factor 3 complex / OAS antiviral response / formation of cytoplasmic translation initiation complex / cytoplasmic translational initiation / multi-eIF complex / translation factor activity, RNA binding / eukaryotic 43S preinitiation complex / mRNA cap binding / eukaryotic 48S preinitiation complex / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / negative regulation of peptidyl-serine phosphorylation / positive regulation of respiratory burst involved in inflammatory response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / protein tyrosine kinase inhibitor activity / IRE1-RACK1-PP2A complex / positive regulation of endodeoxyribonuclease activity / nucleolus organization / positive regulation of Golgi to plasma membrane protein transport / translation at postsynapse / ribosome disassembly / TNFR1-mediated ceramide production / ribosomal subunit / negative regulation of DNA repair / negative regulation of RNA splicing / metal-dependent deubiquitinase activity / mammalian oogenesis stage / regulation of translational initiation / supercoiled DNA binding / activation-induced cell death of T cells / neural crest cell differentiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / NF-kappaB complex / oxidized purine DNA binding / cysteine-type endopeptidase activator activity involved in apoptotic process / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / ubiquitin-like protein conjugating enzyme binding / regulation of establishment of cell polarity / translation at presynapse / positive regulation of ubiquitin-protein transferase activity / Formation of the ternary complex, and subsequently, the 43S complex / negative regulation of phagocytosis / erythrocyte homeostasis / rRNA modification in the nucleus and cytosol / cytoplasmic side of rough endoplasmic reticulum membrane / laminin receptor activity / protein kinase A binding / negative regulation of ubiquitin protein ligase activity / pigmentation / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / positive regulation of mitochondrial depolarization / positive regulation of T cell receptor signaling pathway / positive regulation of activated T cell proliferation / fibroblast growth factor binding / negative regulation of Wnt signaling pathway / monocyte chemotaxis / negative regulation of translational frameshifting / Protein hydroxylation / BH3 domain binding / TOR signaling / SARS-CoV-1 modulates host translation machinery / regulation of cell division / mTORC1-mediated signalling / T cell proliferation involved in immune response / Peptide chain elongation / iron-sulfur cluster binding / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal small subunit binding / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of ubiquitin-dependent protein catabolic process / Viral mRNA Translation / negative regulation of respiratory burst involved in inflammatory response / phagocytic cup / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / erythrocyte development / endoribonuclease inhibitor activity

Domain/homology:

Eukaryotic translation initiation factor 3 subunit J / Eukaryotic translation initiation factor 3-like domain superfamily / Translation initiation factor eIF3 subunit / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / RLI, domain 1 / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / EIF3I / RLI1 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit 6 N terminal domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / RNase L inhibitor RLI-like, possible metal-binding domain / Possible Fer4-like domain in RNase L inhibitor, RLI / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor SUI1 / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor eIF2A / SUI1 domain superfamily / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / SUI1 domain / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / : / eIF3a, PCI domain, TPR-like region / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / 4Fe-4S binding domain / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / S27a-like superfamily / 40S Ribosomal protein S10 / : / Ribosomal protein S7e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S17e, conserved site / Ribosomal protein S27a / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S2, eukaryotic/archaeal / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27e signature.

Component:

Ribosomal protein S14 / Eukaryotic translation initiation factor 3 subunit C / 40S ribosomal protein S24 / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Eukaryotic translation initiation factor 3 subunit J / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Eukaryotic translation initiation factor 1 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein uS10 / ATP-binding cassette sub-family E member 1 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.8 Å
• Authors: Kratzat H / Mackens-Kiani T

Funding support

Germany, France, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany
Centre National de la Recherche Scientifique (CNRS)		France

• Citation: Journal: EMBO J / Year: 2021; Title: A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes.; Authors: Hanna Kratzat / Timur Mackens-Kiani / Michael Ameismeier / Mia Potocnjak / Jingdong Cheng / Estelle Dacheux / Abdelkader Namane / Otto Berninghausen / Franz Herzog / Micheline Fromont-Racine / Thomas Becker / Roland Beckmann / ; Abstract: In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. These comprise/include the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of the next 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S, but also with later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide-binding domains, while interacting with the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus providing a structural explanation for its antagonistic role with respect to mRNA binding. Overall, the native human samples provide a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and the eIF2 ternary complex containing the initiator tRNA.

History

Deposition	Jul 24, 2020	-
Header (metadata) release	Oct 7, 2020	-
Map release	Oct 7, 2020	-
Update	May 1, 2024	-
Current status	May 1, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_11458.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.059 Å

Density

Contour Level	By AUTHOR: 0.015 / Movie #1: 0.015
Minimum - Maximum	-0.03345038 - 0.086971
Average (Standard dev.)	-0.0000085757565 (±0.004475016)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 423.6 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.059	1.059	1.059
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	423.600	423.600	423.600
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	162	182	277
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.033	0.087	-0.000

Supplemental data

Additional map: post processed and masked map

• File: emd_11458_additional_1.map
• Annotation: post processed and masked map

Additional map: local resolution filtered map

• File: emd_11458_additional_2.map
• Annotation: local resolution filtered map

Additional map: multi body refinement body 1

• File: emd_11458_additional_3.map
• Annotation: multi body refinement body 1

Additional map: multi body refinement body 2

• File: emd_11458_additional_4.map
• Annotation: multi body refinement body 2

Additional map: multi body refinement body 3

• File: emd_11458_additional_5.map
• Annotation: multi body refinement body 3

Additional map: multi body refinement if eIF3 core body 1

• File: emd_11458_additional_6.map
• Annotation: multi body refinement if eIF3 core body 1

Additional map: multi body refinement if eIF3 core body 2

• File: emd_11458_additional_7.map
• Annotation: multi body refinement if eIF3 core body 2

Sample components

Entire : ABCE1-bound 43S initiation complex

• Entire: Name: ABCE1-bound 43S initiation complex

Components

• Complex: ABCE1-bound 43S initiation complex
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S30
  • RNA: 18S ribosomal RNA
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit I
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit B
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit C
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit F
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit H
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit K
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit L
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit M
  • Protein or peptide: ATP-binding cassette sub-family E member 1
  • Protein or peptide: Eukaryotic translation initiation factor 1
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit D
  • Protein or peptide: RNA recognition motif (unknown)
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit J
• Ligand: ZINC ION
• Ligand: IRON/SULFUR CLUSTER
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE

Supramolecule #1: ABCE1-bound 43S initiation complex

• Supramolecule: Name: ABCE1-bound 43S initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#50
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.260758 KDa

Sequence

String:

SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKF AAATGATPIA GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT DSPLRYVDIA I PCNNKGAH SVGLMWWMLA REVLRMRGTI SREHPWEVMP DLYFYRDPEE IEKEEQAA

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #2: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.560883 KDa

Sequence

String:

DPFSKKDWYD VKAPAMFNIR NIGKTLVTRT QGTKIASDGL KGRVFEVSLA DLQNDEVAFR KFKLITEDVQ GKNCLTNFHG MDLTRDKMC SMVKKWQTMI EAHVDVKTTD GYLLRLFCVG FTKKRNNQIR KTSYAQHQQV RQIRKKMMEI MTREVQTNDL K EVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHG

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #3: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.861238 KDa

Sequence

String:

WMPVTKLGRL VKDMKIKSLE EIYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVA TAIRGAIILA KLSIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG I DDCYTSAR GCTATLGNFA KATFDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVK

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #4: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.645794 KDa

Sequence

String:

TKKRRNNGRA KKGRGHVQPI RCTNCARCVP KDKAIKKFVI RNIVEAAAVR DISEASVFDA YVLPKLYVKL HYCVSCAIHS KVVRNRSRE ARKDRTPPPR FR

UniProtKB: Small ribosomal subunit protein eS26

Macromolecule #5: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.892998 KDa

Sequence

String:

ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNR LKYALTGDEV KKICMQRFIK IDGKVRTDIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI Q IDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RG KGIRLTI AEERDKRL

UniProtKB: Small ribosomal subunit protein eS4, X isoform

Macromolecule #6: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 3.344271 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRS

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #7: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.612301 KDa

Sequence

String:

KLNISFPATG CQKLIEVDDE RKLRTFYEKR MATEVAADAL GEEWKGYVVR ISGGNDKQGF PMKQGVLTHG RVRLLLSKGH SCYRPRRTG ERKRKSVRGC IVDANLSVLN LVIVKKGEKD IPGLTDTTVP RRLGPKRASR IRKLFNLSKE DDVRQYVVRK P LNKEGKKP RTKAPKIQRL VTPRVLQHKR RRIALKKQRT KKNKEEAAEY AKLLAKRMKE AKEK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #8: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.788342 KDa

Sequence

String:

IVKPNEFESG ISQALLELEM NSDLKAQLRE LNITAAKEIE VGGGRKAIII FVPVPQLKSF QKIQVRLVRE LEKKFSGKHV VFIAQRRIL PKPTRKSRTK NKQKRPRSRT LTAVHDAILE DLVFPSEIVG KRIRVKLDGS RLIKVHLDKA QQNNVEHKVE T FSGVYKKL TGKDVNFEFP EFQ

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #9: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.945959 KDa

Sequence

String:

GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #10: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.279271 KDa

Sequence

String:

PVARSWVCRK TYVTPRRPFE KSRLDQELKL IGEYGLRNKR EVWRVKFTLA KIRKAARELL TLDEKDPRRL FEGNALLRRL VRIGVLDEG KMKLDYILGL KIEDFLERRL QTQVFKLGLA KSIHHARVLI RQRHIRVRKQ VVNIPSFIVR LDSQKHIDFS L RSPYGGGR PGRVKRKNAK KG

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #11: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.913004 KDa

Sequence

String:

IQTERAYQKQ PTIFQNKKRV LLGETGKEKL PRYYKNIGLG FKTPKEAIEG TYIDKKCPFT GNVSIRGRIL SGVVTKMKMQ RTIVIRRDY LHYIRKYNRF EKRHKNMSVH LSPCFRDVQI GDIVTVGECR PLSKTVRFNV LKVTK

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #12: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.057113 KDa

Sequence

String:

GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #13: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.463481 KDa

Sequence

String:

NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQRC KELGITALHI KLRATGGNRT KTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRR

UniProtKB: Ribosomal protein S14

Macromolecule #14: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.977214 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KN

UniProtKB: Small ribosomal subunit protein eS21

Macromolecule #15: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.734357 KDa

Sequence

String:

VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFDV QLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #16: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.372082 KDa

Sequence

String:

GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #17: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.259944 KDa

Sequence

String:

VTIRTRKFMT NRLLQRKQMV IDVLHPGKAT VPKTEIREKL AKMYKTTPDV IFVFGFRTHF GGGKTTGFGM IYDSLDYAKK NEPKHRLAR HGLYEKKKTS RKQRKERKNR MKKVRGTAKA NVG

UniProtKB: 40S ribosomal protein S24

Macromolecule #18: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.210843 KDa

Sequence

String:

PLAKDLLHPS PEEEKRKHKK KRLVQSPNSY FMDVKCPGCY KITTVFSHAQ TVVLCVGCST VLCQPTGGKA RLTEGCSFRR KQ

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #19: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 5.069001 KDa

Sequence

String:

ARAGKVRGQT PKVAKQEKKK KKTGRAKRRM QYNRRFVNVG PNAN

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #21: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.036469 KDa

Sequence

String:

GRVRTKTVKK AARVIIEKYY TRLGNDFHTN KRVCEEIAII PSKKLRNKIA GYVTHLMKRI QRGPVRGISI KLQEEERERR DNYVPEVSA LDQEIIEVDP DTKEMLKLLD FGSLSNLQVT QPTVGMNFKT P

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #22: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.637354 KDa

Sequence

String:

SVQVFGRKKT ATAVAHCKRG NGLIKVNGRP LEMIEPRTLQ YKLLEPVLLL GKERFAGVDI RVRVKGGGHV AQIYAIRQSI SKALVAYYQ KYVDEASKKE IKDILIQYDR TLLVADPRRC ESKKFGGPGA RARYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #23: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.871156 KDa

Sequence

String:

QISKKRKFVA DGIFKAELNE FLTRELAEDG YSGVEVRVTP TRTEIIILAT RTQNVLGEKG RRIRELTAVV QKRFGFPEGS VELYAEKVA TRGLCAIAQA ESLRYKLLGG LAVRRACYGV LRFIMESGAK GCEVVVSGKL RGQRAKSMKF VDGLMIHSGD P VNYYVDTA VRHVLLRQGV LGIKVKIMLP WDPTGKIGPK KPLPDHVSIV EPKDEILPTT PISEQK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #24: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.327723 KDa

Sequence

String:

DIKLFGKWST DDVQINDISL QDYIAVKEKY AKYLPHSAGR YAAKRFRKAQ CPIVERLTNS MMMHGRNNGK KLMTVRIVKH AFEIIHLLT GENPLQVLVN AIINSGPRED STRIGRAGTV RRQAVDVSPL RRVNQAIWLL CTGAREAAFR NIKTIAECLA D ELINAAKG SSNSYAIKKK DELERVAKSN R

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #25: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.372488 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLR

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #26: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.945101 KDa

Sequence

String:

VNTALQEVLK TALIHDGLAR GIREAAKALD KRQAHLCVLA SNCDEPMYVK LVEALCAEHQ INLIKVDDNK KLGEWVGLCK IDREGKPRK VVGCSCVVVK DYGKESQAKD VIEEYFK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #27: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.01054 KDa

Sequence

String:

FTYRGVDLDQ LLDMSYEQLM QLYSARQRRR LNRGLRRKQH SLLKRLRKAK KEAPPMEKPE VVKTHLRDMI ILPEMVGSMV GVYNGKTFN QVEIKPEMIG HYLGEFSITY KPVKHGRPGI

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #28: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.496223 KDa

Sequence

String:

LVIPEKFQHI LRVLNTNIDG RRKIAFAITA IKGVGRRYAH VVLRKADIDL TKRAGELTED EVERVITIMQ NPRQYKIPDW FLNRQKDVK DGKYSQVLAN GLDNKLREDL ERLKKIRAHR GLRHFWGLRV RGQHTKTTGR R

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #29: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.563858 KDa

Sequence

String:

PGVTVKDVNQ QEFVRALAAF LKKSGKLKVP EWVDTVKLAK HKELAPYDEN WFYTRAASTA RHLYLRGGAG VGSMTKIYGG RQRNGVMPS HFSRGSKSVA RRVLQALEGL KMVEKDQDGG RKLTPQGQRD LDRIAGQVAA AN

UniProtKB: Small ribosomal subunit protein eS19

Macromolecule #30: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.186265 KDa

Sequence

String:

RIRITLTSRN VKSLEKVCAD LIRGAKEKNL KVKGPVRMPT KTLRITTRKT PCGEGSKTWD RFQMRIHKRL IDLHSPSEIV KQITSISIE PGVEVEVTI

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #31: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.938426 KDa

Sequence

String:

LNNLVLFDKA TYDKLCKEVP NYKLITPAVV SERLKIRGSL ARAALQELLS KGLIKLVSKH RAQVIYTRNT

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #32: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.87894 KDa

Sequence

String:

PIKLARVTKV LGRTGSQGQC TQVRVEFMDD TSRSIIRNVK GPVREGDVLT LLESEREARR L

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #33: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.444537 KDa

Sequence

String:

GHQQLYWSHP RKFGQGSRSC RVCSNRHGLI RKYGLNMCRQ CFRQYAKDIG FIKL

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #34: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 7.257627 KDa

Sequence

String:

PKKNKHKRKK VKLAVLKYYK VDENGKISRL RRECPSDECG AGVFMASHFD RHYCGKCCLT YC

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #35: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.224641 KDa

Sequence

String:

QMTLRGTLKG HNGWVTQIAT TPQFPDMILS ASRDKTIIMW KLTRDETNYG IPQRALRGHS HFVSDVVISS DGQFALSGSW DGTLRLWDL TTGTTTRRFV GHTKDVLSVA FSSDNRQIVS GSRDKTIKLW NTLGVCKYTV QDESHSEWVS CVRFSPNSSN P IIVSCGWD KLVKVWNLAN CKLKTNHIGH TGYLNTVTVS PDGSLCASGG KDGQAMLWDL NEGKHLYTLD GGDIINALCF SP NRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QV

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #36: Eukaryotic translation initiation factor 3 subunit I

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.352535 KDa

Sequence

String:

MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLAL LKTNSAVRTC GFDFGGNIIM FSTDKQMGYQ CFVSFFDLRD PSQIDNNEPY MKIPCNDSKI TSAVWGPLGE C IIAGHESG ELNQYSAKSG EVLVNVKEHS RQINDIQLSR DMTMFVTASK DNTAKLFDST TLEHQKTFRT ERPVNSAALS PN YDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDP

UniProtKB: Eukaryotic translation initiation factor 3 subunit I

Macromolecule #37: Eukaryotic translation initiation factor 3 subunit B

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.901969 KDa

Sequence

String:

ADGIDSVIVV DNVPQVGPDR LEKLKNVIHK IFSKFGKITN DFYPEEDGKT KGYIFLEYAS PAHAVDAVKN ADGYKLDKQH TFRVNLFTD FDKYMTISDE WDIPEKQPFK DLGNLRYWLE EAECRDQYSV IFESGDRTSI FWNDVKDPVS IEERARWTET Y VRWSPKGT YLATFHQRGI ALWGGEKFKQ IQRFSHQGVQ LIDFSPCERY LVTFSPLMDT QDDPQAIIIW DILTGHKKRG FH CESSAHW PIFKWSHDGK FFARMTLDTL SIYETPSMGL LDKKSLKISG IKDFSWSPGG NIIAFWVPED KDIPARVTLM QLP TRQEIR VRNLFNVVDC KLHWQKNGDY LCVKVDRTPK GTQGVVTNFE IFRMREKQVP VDVVEMKETI IAFAWEPNGS KFAV LHGEA PRISVSFYHV KNNGKIELIK MFDKQQANTI FWSPQGQFVV LAGLRSMNGA LAFVDTSDCT VMNIAEHYMA SDVEW DPTG RYVVTSVSWW SHKVDNAYWL WTFQGRLLQK NNKDRFCQLL WRPRPPTLLS QEQIKQIKKD LKKYSKIFEQ KDRLSQ SKA SKE

UniProtKB: Eukaryotic translation initiation factor 3 subunit B

Macromolecule #38: Eukaryotic translation initiation factor 3 subunit A

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 85.564188 KDa

Sequence

String:

YFQRPENALK RANEFLEVGK KQPALDVLYD VMKSKKHRTW QKIHEPIMLK YLELCVDLRK SHLAKEGLYQ YKNICQQVNI KSLEDVVRA YLKMAEEKTE AAKEESQQMV LDIEDLDNIQ TPESVLLSAV SGEDTQDRTD RLLLTPWVKF LWESYRQCLD L LRNNSRVE RLYHDIAQQA FKFCLQYTRK AEFRKLCDNL RMHLSQIQRH HNQSTAINLN NPESQSMHLE TRLVQLDSAI SM ELWQEAF KAVEDIHGLF SLSKKPPKPQ LMANYYNKVS TVFWKSGNAL FHASTLHRLY HLSREMRKNL TQDEMQRMST RVL LATLSI PITPERTDIA RLLDMDGIIV EKQRRLATLL GLQAPPTRIG LINDMVRFNV LQYVVPEVKD LYNWLEVEFN PLKL CERVT KVLNWVREQP EKEPELQQYV PQLQNNTILR LLQQVSQIYQ SIEFSRLTSL VPFVDAFQLE RAIVDAARHC DLQVR IDHT SRTLSFGSDL NYATREDAPI GPHLQSMPSE QIRNQLTAMS SVLAKALEVI KPAHILQEKE EQHQLAVTAY LKNSRK EHQ RILARRQTIE ERKERLESLN IQREKEELEQ REAELQKVRK AEEERLRQEA KEREKERILQ EHEQIKKKTV RERLEQI KK TELGAKAFKD IDIEDLEELD PDFIMAKQVE QLEKEKKELQ ERLKNQEKKI DYFERAKRLE EIPLIKSAYE EQRIKDMD L W

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

Macromolecule #39: Eukaryotic translation initiation factor 3 subunit C

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 86.19757 KDa

Sequence

String:

MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN KGTEITHAVV I KKLNEILQ ARGKKGTDRA AQIELLQLLV QIAAENNLGE GVIVKIKFNI IASLYDYNPN LATYMKPEMW GKCLDCINEL MD ILFANPN IFVGENILEE SENLHNADQP LRVRGCILTL VERMDEEFTK IMQNTDPHSQ EYVEHLKDEA QVCAIIERVQ RYL EEKGTT EEVCRIYLLR ILHTYYKFDY KAHQRQLTPP EGSSKSEQDQ AENEGEDSAV LMERLCKYIY AKDRTDRIRT CAIL CHIYH HALHSRWYQA RDLMLMSHLQ DNIQHADPPV QILYNRTMVQ LGICAFRQGL TKDAHNALLD IQSSGRAKEL LGQGL LLRS LQERNQEQEK VERRRQVPFH LHINLELLEC VYLVSAMLLE IPYMAAHESD ARRRMISKQF HHQLRVGERQ PLLGPP ESM REHVVAASKA MKMGDWKTCH SFIINEKMNG KVWDLFPEAD KVRTMLVRKI QEESLRTYLF TYSSVYDSIS METLSDM FE LDLPTVHSII SKMIINEELM ASLDQPTQTV VMHRTEPTAQ QNLALQLAEK LGSLVENNER VFDHKQGTYG GYFRDQKD G YRKNEGYMRR GGYRQQQSQT AY

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

Macromolecule #40: Eukaryotic translation initiation factor 3 subunit E

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 50.43868 KDa

Sequence

String:

AEYDLTTRIA HFLDRHLVFP LLEFLSVKEI YNEKELLQGK LDLLSDTNMV DFAMDVYKNL YSDDIPHALR EKRTTVVAQL KQLQAETEP IVKMFEDPET TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY LYFFRVLVPA T DRNALSSL WGKLASEILM QNWDAAMEDL TRLKETIDNN SVSSPLQSLQ QRTWLIHWSL FVFFNHPKGR DNIIDLFLYQ PQ YLNAIQT MCPHILRYLT TAVITNKDVR KRRQVLKDLV KVIQQESYTY KDPITEFVEC LYVNFDFDGA QKKLRECESV LVN DFFLVA CLEDFIENAR LFIFETFCRI HQCISINMLA DKLNMTPEEA ERWIVNLIRN ARLDAKIDSK LGHVVMGNNA VSPY QQVIE KTKSLSFRSQ MLAMNIEKKL NQNS

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

Macromolecule #41: Eukaryotic translation initiation factor 3 subunit F

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO / EC number: ubiquitinyl hydrolase 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.836004 KDa

Sequence

String:

GRVVRLHPVI LASIVDSYER RNEGAARVIG TLLGTVDKHS VEVTNCFSVP HNESEDEVAV DMEFAKNMYE LHKKVSPNEL ILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI G VDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG KVSADNTVGR FLMSLVNQVP KIVPDDFETM LN SNINDLL MVTYLANLTQ SQIALNEKLV NL

UniProtKB: Eukaryotic translation initiation factor 3 subunit F

Macromolecule #42: Eukaryotic translation initiation factor 3 subunit H

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 36.960078 KDa

Sequence

String:

AVKQVQIDGL VVLKIIKHYQ EEGQGTEVVQ GVLLGLVVED RLEITNCFPF PQHTEDDADF DEVQYQMEMM RSLRHVNIDH LHVGWYQST YYGSFVTRAL LDSQFSYQHA IEESVVLIYD PIKTAQGSLS LKAYRLTPKL MEVCKEKDFS PEALKKANIT F EYMFEEVP IVIKNSHLIN VLMWELEKKS AVADKHELLS LASSNHLGKN LQLLMDRVDE MSQDIVKYNT YMRNTSKQQQ QK HQYQQRR QQENMQRQSR GEPPLPEEDL SKLFKPPQPP ARMDSLLIAG QINTYCQNIK EFTAQNLGKL FMAQALQEYN N

UniProtKB: Eukaryotic translation initiation factor 3 subunit H

Macromolecule #43: Eukaryotic translation initiation factor 3 subunit K

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.952424 KDa

Sequence

String:

AMFEQMRANV GKLLKGIDRY NPENLATLER YVETQAKENA YDLEANLAVL KLYQFNPAFF QTTVTAQILL KALTNLPHTD FTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE M LGDLSDSQ LKVWMSKYGW SADESGQIFI CSQEESIKPK NIVEKIDFDS VSSIMASSQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit K

Macromolecule #44: Eukaryotic translation initiation factor 3 subunit L

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.172688 KDa

Sequence

String:

QWLWDIIDEF IYQFQSFSQY RCKTAKKSEE EIDFLRSNPK IWNVHSVLNV LHSLVDKSNI NRQLEVYTSG GDPESVAGEY GRHSLYKML GYFSLVGLLR LHSLLGDYYQ AIKVLENIEL NKKSMYSRVP ECQVTTYYYV GFAYLMMRRY QDAIRVFANI L LYIQRTKS MFQRTTYKYE MINKQNEQMH ALLAIALTMY PMRIDESIHL QLREKYGDKM LRMQKGDPQV YEELFSYSCP KF LSPVVPN YDNVHPNYHK EPFLQQLKVF SDEVQQQAQL STIRSFLKLY TTMPVAKLAG FLDLTEQEFR IQLLVFKHKM KNL VWTSGI SALDGEFQSA SEVDFYIDKD MIHIADTKVA RRYGDFFIRQ IHKFEE

UniProtKB: Eukaryotic translation initiation factor 3 subunit L

Macromolecule #45: Eukaryotic translation initiation factor 3 subunit M

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.278391 KDa

Sequence

String:

EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEKL VKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE KKHTLLRLLY E ALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DLLTIFVSAK LA SYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKMVYCKIDQ TQR KVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLS

UniProtKB: Eukaryotic translation initiation factor 3 subunit M

Macromolecule #46: ATP-binding cassette sub-family E member 1

• Macromolecule: Name: ATP-binding cassette sub-family E member 1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 66.972789 KDa

Sequence

String:

KLTRIAIVNH DKCKPKKCRQ ECKKSCPVVR MGKLCIEVTP QSKIAWISET LCIGCGICIK KCPFGALSIV NLPSNLEKET THRYCANAF KLHRLPIPRP GEVLGLVGTN GIGKSTALKI LAGKQKPNLG KYDDPPDWQE ILTYFRGSEL QNYFTKILED D LKAIIKPQ YVDQIPKAAK GTVGSILDRK DETKTQAIVC QQLDLTHLKE RNVEDLSGGE LQRFACAVVC IQKADIFMFD EP SSYLDVK QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT MPFSVREGIN IFLDGYVPTE NLR FRDASL VFKVAETANE EEVKKMCMYK YPGMKKKMGE FELAIVAGEF TDSEIMVMLG ENGTGKTTFI RMLAGRLKPD EGGE VPVLN VSYKPQKISP KSTGSVRQLL HEKIRDAYTH PQFVTDVMKP LQIENIIDQE VQTLSGGELQ RVALALCLGK PADVY LIDE PSAYLDSEQR LMAARVVKRF ILHAKKTAFV VEHDFIMATY LADRVIVFDG VPSKNTVANS PQTLLAGMNK FLSQLE ITF RRDPNNYRPR INKLNSIKDV EQKKSGNYFF LD

UniProtKB: ATP-binding cassette sub-family E member 1

Macromolecule #47: Eukaryotic translation initiation factor 1

• Macromolecule: Name: Eukaryotic translation initiation factor 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.388935 KDa

Sequence

String:

LPAGTEDYIH IRIQQRNGRK TLTTVQGIAD DYDKKKLVKA FKKKFACNGT VIEHPEYGEV IQLQGDQRKN ICQFLVEIGL AKDDQLKVH GF

UniProtKB: Eukaryotic translation initiation factor 1

Macromolecule #48: Eukaryotic translation initiation factor 3 subunit D

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 61.489441 KDa

Sequence

String:

MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY QDKRYTNKYS SQFGGGSQYA YFHEEDESSF QLVDTARTQ KTAYQRNRMR FAQRNLRRDK DRRNMLQFNL QILPKSAKQK ERERIRLQKK FQKQFGVRQK WDQKSQKPRD S SVEVRSDW EVKEEMDFPQ LMKMRYLEVS EPQDIECCGA LEYYDKAFDR ITTRSEKPLR SIKRIFHTVT TTDDPVIRKL AK TQGNVFA TDAILATLMS CTRSVYSWDI VVQRVGSKLF FDKRDNSDFD LLTVSETANE PPQDEGNSFN SPRNLAMEAT YIN HNFSQQ CLRMGKERYN FPNPNPFVED DMDKNEIASV AYRYRRWKLG DDIDLIVRCE HDGVMTGANG EVSFINIKTL NEWD SRHCN GVDWRQKLDS QRGAVIATEL KNNSYKLARW TCCALLAGSE YLKLGYVSRY HVKDSSRHVI LGTQQFKPNE FASQI NLSV ENAWGILRCV IDICMKLEEG KYLILKDPNK QVIRVYSLPD GTF

UniProtKB: Eukaryotic translation initiation factor 3 subunit D

Macromolecule #49: RNA recognition motif (unknown)

• Macromolecule: Name: RNA recognition motif (unknown) / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 5.562077 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAA

Macromolecule #50: Eukaryotic translation initiation factor 3 subunit J

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit J / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.108453 KDa

Sequence

String:

MAAAAAAAGD SDSWDADAFS VEDPVRKVGG GGTAGGDRWE GEDEDEDVKD NWDDDDDEKK EEAEVKPEVK ISEKKKIAEK IKEKERQQK KRQEEIKKRL EEPEEPKVLT PEEQLADKLR LKKLQEESDL ELAKETFGVN NAVYGIDAMN PSSRDDFTEF G KLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DL ADYGGYD GGYVQDYEDF M

UniProtKB: Eukaryotic translation initiation factor 3 subunit J

Macromolecule #20: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 20 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 555.210688 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GUACUUGGAU AACUGUGGUA AUUCUAGAGC U AAUACAUG CCGACGGGCG CUGACCCCCU UCGCGGGGGG GAUGCGUGCA UUUAUCAGUG GUGACUCUAG AUAACCUCGG GC CGAUCGC ACGCCGCGGC GACGACCCAU UCGAACGUCU GCCCUAUCAA CUUUCGAUGG UAGUCGCCGU GCCUACCAUG GUG ACCACG GGUGACGGGG AAUCAGGGUU CGAUUCCGGA GAGGGAGCCU GAGAAACGGC UACCACAUCC AAGGAAGGCA GCAG GCGCG CAAAUUACCC ACUCCCGACC CGGGGAGGUA GUGACGAAAA AUAACAAUAC AGGACUCUUU CGAGGCCCUG UAAUU GGAA UGAGUCCACU UUAAAUCCUU UAACGAGGAU CCAUUGGAGG GCAAGUCUGG UGCCAGCAGC CGCGGUAAUU CCAGCU CCA AUAGCGUAUA UUAAAGUUGC UGCAGUUAAA AAGCUCGUAG UUGGAUCUUG GGAGCGGGCG GGCGGUCCGC CGCGAGG CG AGCCACCGCC CGUCCCCGCC UCUCGGCCGA AGCGUUUACU UUGAAAAAAU UAGAGUGUUC AAAGCAGGCC GCCUGGAU A CCGCAGCUAG GAAUAAUGGA AUAGGACCGC GGUUCUAUUU UGUUGGUUUU CGGAACUGAG GCCAUGAUUA AGAGGGACG GCCGGGGGCA UUCGUAUUGC GCCGCUAGAG GUGAAAUUCU UGGACCGGCG CAAGACGGAC CAGAGCGAAA GCAUUUGCCA AGAAUGUUU UCAUUAAUCA AGAACGAAAG UCGGAGGUUC GAAGACGAUC AGAUACCGUC GUAGUUCCGA CCAUAAACGA U GCCGACCG GCGAUGCGGC GGCGUUAUUC CCAUGACCCG CCGGGCAGCU UCCGGGAAAC CAAAGUCUUU GGGUUCCGGG GG GAGUAUG GUUGCAAAGC UGAAACUUAA AGGAAUUGAC GGAAGGGCAC CACCAGGAGU GGAGCCUGCG GCUUAAUUUG ACU CAACAC GGGAAACCUC ACCCGGCCCG GACACGGACA GGAUUGACAG AUUGAUAGCU CUUUCUCGAU UCCGUGGGUG GUGG UGCAU GGCCGUUCUU AGUUGGUGGA GCGAUUUGUC UGGUUAAUUC CGAUAACGAA CGAGACUCUG GCAUGCUAAC UAGUU ACGC GACCGGUCGG CGUAACUUCU UAGAGGGACA AGUGGCGUUC AGCCACCCGA GAUUGAGCAA UAACAGGUCU GUGAUG CCC UUAGAUGUCC GGGGCUGCAC GCGCGCUACA CUGACUGGCU CAGCGUGUGC CUACCCUACG CCGGCAGGCG CGGGUAA CC CGUUGAACCC CAUUCGUGAU GGGGAUCGGG GAUUGCAAUU AUUCCCCAUG AACGAGGAAU UCCCAGUAAG UGCGGGUC A UAAGCUUGCG UUGAUUAAGU CCCUGCCCUU UGUACACACC GCCCGUCGCU ACUACCGAUU GGAUGGUUUA GUGAGGCCC UCGGAUCGGC CCCGCCGGGG UGCCCUGGCG GAGCGCUGAG AAGACGGUCG AACUUGACUA UCUAGAGGAA GUAAAAGUCG UAACAAGGU UUCCGUAGGU AAACCUGCGG AAGGAUCAUU A

Macromolecule #51: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 51 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #52: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 52 / Number of copies: 2 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #53: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 53 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #54: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #55: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 55 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 48.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: PDB ENTRY
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 5231
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD