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- EMDB-11242: Membrane domain of open complex I during turnover -

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Basic information

Entry
Database: EMDB / ID: EMD-11242
TitleMembrane domain of open complex I during turnover
Map dataOversampled, local resolution-filtered map
Sample
  • Complex: Membrane domain of open complex I during turnover
    • Protein or peptide: x 29 types
  • Ligand: x 8 types
Keywordscomplex / respiration / NADH / proton pump / mitochondria / iron-sulphur cluster / oxidoreductase / membrane protein / ELECTRON TRANSPORT
Function / homology
Function and homology information


: / : / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / respiratory chain complex I ...: / : / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / respiratory chain complex I / membrane => GO:0016020 / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / reactive oxygen species metabolic process / electron transport chain / mitochondrial intermembrane space / mitochondrial inner membrane / mitochondrial matrix / mitochondrion
Similarity search - Function
NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial ...NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone oxidoreductase chain 4L/K / NADH-quinone oxidoreductase, chain M/4 / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-quinone oxidoreductase, chain 5-like / Complex 1 LYR protein domain / NADH:ubiquinone oxidoreductase / Complex 1 protein (LYR family) / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / NADH:quinone oxidoreductase/Mrp antiporter, TM / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / CHCH / CHCH domain / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 ...NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
Similarity search - Component
Biological speciesOvis aries (sheep)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsKampjut D / Sazanov LA
Funding supportEuropean Union, 2 items
OrganizationGrant numberCountry
European Commission665385European Union
European Commission653706European Union
CitationJournal: Science / Year: 2020
Title: The coupling mechanism of mammalian respiratory complex I.
Authors: Domen Kampjut / Leonid A Sazanov /
Abstract: Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different ...Mitochondrial complex I couples NADH:ubiquinone oxidoreduction to proton pumping by an unknown mechanism. Here, we present cryo-electron microscopy structures of ovine complex I in five different conditions, including turnover, at resolutions up to 2.3 to 2.5 angstroms. Resolved water molecules allowed us to experimentally define the proton translocation pathways. Quinone binds at three positions along the quinone cavity, as does the inhibitor rotenone that also binds within subunit ND4. Dramatic conformational changes around the quinone cavity couple the redox reaction to proton translocation during open-to-closed state transitions of the enzyme. In the induced deactive state, the open conformation is arrested by the ND6 subunit. We propose a detailed molecular coupling mechanism of complex I, which is an unexpected combination of conformational changes and electrostatic interactions.
History
DepositionJun 30, 2020-
Header (metadata) releaseOct 7, 2020-
Map releaseOct 7, 2020-
UpdateApr 9, 2025-
Current statusApr 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6zka
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6zka
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11242.png

Downloads & links

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Map

FileDownload / File: emd_11242.map.gz / Format: CCP4 / Size: 189.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationOversampled, local resolution-filtered map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.5 Å/pix.
x 499 pix.
= 249.5 Å		0.5 Å/pix.
x 307 pix.
= 153.5 Å		0.5 Å/pix.
x 325 pix.
= 162.5 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 0.5 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.15 / Movie #1: 0.15
Minimum - Maximum-0.47310528 - 0.8375051
Average (Standard dev.)0.009621947 (±0.056857377)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions307325499
Spacing325307499
CellA: 162.5 Å / B: 153.5 Å / C: 249.5 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.50.50.5
M x/y/z325307499
origin x/y/z0.0000.0000.000
length x/y/z162.500153.500249.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS325307499
D min/max/mean-0.4730.8380.010

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Supplemental data

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Sample components

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Entire : Membrane domain of open complex I during turnover

EntireName: Membrane domain of open complex I during turnover
Components
  • Complex: Membrane domain of open complex I during turnover
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
    • Protein or peptide: Mitochondrial complex I, B14.7 subunit
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B5
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
    • Protein or peptide: Mitochondrial complex I, PDSW subunit
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit S5
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit A3
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B3
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B4
    • Protein or peptide: Mitochondrial complex I, B16.6 subunit
    • Protein or peptide: Mitochondrial complex I, B17 subunit
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B7
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B9
    • Protein or peptide: NADH:ubiquinone oxidoreductase subunit B2
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
    • Protein or peptide: Mitochondrial complex I, ESSS subunit
    • Protein or peptide: Mitochondrial complex I, KFYI subunit
    • Protein or peptide: Mitochondrial complex I, MNLL subunit
    • Protein or peptide: Mitochondrial complex I, MWFE subunit
    • Protein or peptide: Mitochondrial complex I, ND4L subunit
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: CARDIOLIPIN
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: ADENOSINE MONOPHOSPHATE
  • Ligand: MYRISTIC ACID
  • Ligand: water

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Supramolecule #1: Membrane domain of open complex I during turnover

SupramoleculeName: Membrane domain of open complex I during turnover / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#29
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 13.10652 KDa
SequenceString:
MNLMITLLTN FTLATLLVTI AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TTNLNTMLT MALLLIFLLA VSLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

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Macromolecule #2: NADH-ubiquinone oxidoreductase chain 1

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 35.884902 KDa
SequenceString: MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT ...String:
MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT QEQVWLIFPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNIFT TT LFLGAFH NPYMPELYTI NFTIKSLLLS ITFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILLSSIPPQ T

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

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Macromolecule #3: NADH-ubiquinone oxidoreductase chain 6

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 19.126619 KDa
SequenceString:
MMTYIVFILS IIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMATEQYP EVWVSNKVV LGTFITGLLM EFLMVYYVLK DKEVEIVFKF NGMGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

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Macromolecule #4: NADH-ubiquinone oxidoreductase chain 4L

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.868237 KDa
SequenceString:
(FME)SLVYMNIMM AFTVSLTGLL MYRSHLMSSL LCLEGMMLSL FILATLMILN SHFTLASMMP IILLVFAACE AALGLS LLV MVSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

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Macromolecule #5: NADH-ubiquinone oxidoreductase chain 5

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 68.438906 KDa
SequenceString: (FME)NLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKL SLS FKMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA ...String:
(FME)NLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKL SLS FKMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIG WW YGRTDANTAA LQAILYNRIG DIGFILAMAW FLINLNTWDL QQIFMLNPND SNLPLMGLIL AATGKSAQFG LHPWLPSA M EGPTPVSALL HSSTMVVAGI FLLIRFYPLT ENNKFGQSIM LCLGAMTTLF TAMCALTQND IKKIIAFSTS SQLGLMMVT IGINQPHLAF LHICTHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLFKAM PFTTTALIIG SLALTGMPFL TGFYSKDLII ESANTSYTN AWALLMTLVA TSFTAIYSTR IIFFALLGQP RFPTLININE NNPFLINSIK RLLIGSLFAG FIISNNIPPM T IPQMTMPH YLKMTALTVT ILGFILALEI SNTTHYLKFN YPSNTFKFSN LLGYYPTIMH RLTPYMNLTM SQKSASSLLD LI WLETILP KTISLAQMKM STTITSQKGL IKLYFLSFLI TILISTTLLN FHE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

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Macromolecule #6: NADH-ubiquinone oxidoreductase chain 4

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 52.086742 KDa
SequenceString: (FME)LKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLM LMA SQHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAG SL PLLVALIYIQ ...String:
(FME)LKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLM LMA SQHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAG SL PLLVALIYIQ NTMGSLNFLI LQYWVQPMPN SWSNTFMWLA CMMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAILL K LGGYGMMRIT LLLNPITDFM AYPFIMLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAILIQTPW SYMGATALM IAHGLTSSML FCLANSNYER VHSRTMILAR GLQTLLPLMA AWWLLASLTN LALPPSINLI GELFVVMSTF SWSNITIILM GLNMVITAL YSLYMLITTQ RGKHTHHINN ILPSFTRENA LMSLHMLPLL LLSLNPKIIL GPLY

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

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Macromolecule #7: NADH-ubiquinone oxidoreductase chain 2

MacromoleculeName: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 39.149805 KDa
SequenceString: MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG ...String:
MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLLYNP TMTLLNLIIY IIMTSTMFTL FMANSTTTTL SLSHTWNKAP IM TILVLIT LLSMGGLPPL SGFMPKWMII QEMTKNDSII LPTLMAITAL LNLYFYMRLT YSTALTMFPS TNNMKMKWQF PTT KRMTLL PTMTVLSTML LPLTPILSIL E

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

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Macromolecule #8: Mitochondrial complex I, B14.7 subunit

MacromoleculeName: Mitochondrial complex I, B14.7 subunit / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.784009 KDa
SequenceString:
M(AYA)KTLLHQYW DIPEGTECHR KTYAATSIGG ASGLVVSAYS VALKTPTSFL EGVARTGRYT FTAAAIGAIF GLTSCI SAQ VREKPDDPLN YLIGGCAGGL TLGARTRSYG IGAAACAYMG LTAALVKMGQ LEGWKVFAEP KV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

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Macromolecule #9: NADH:ubiquinone oxidoreductase subunit B5

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 21.614201 KDa
SequenceString:
MAAMSLLHRA SVSAVVALSG RRLGTRLGFG GFLTRDFPKT VAPVRHSGDH GKRLFIIKPS GFYDKRFLKL LRFYILLTGI PVVIGITLI NVFIGEAELA EIPEGYVPEH WEYFKHPISR WIARTFFDAP EKNYERTMAI LQIESEKAEL RLKELEVRRL M RAKGDGPW FQYPTIDKAL IDHSPKATPD N

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

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Macromolecule #10: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 17.608199 KDa
SequenceString:
VEVKDFYTTN YQTAVSFSPL GPMPSMALMA VSLSGANVPK SGGRPEESRV PVLTQRKVPG RVTPLCRQYS DAPPLTLEGI KDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

UniProtKB: Acyl carrier protein

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Macromolecule #11: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.139209 KDa
SequenceString:
MPGIVELPSL EDLKVQEVKV SSSVLKAAAH HYGAQCDKPN KEFMLCRWEE KDPRRCLEEG KLVNQCALEF FRQIKRHCAE PFTEYWTCI DYSGLQLFRR CRKEQAQFDK CVLDKLGWVR PDLGELSKVT KVKTDRPLPE NPYHSRARPE PNPEVEGDLK P ARHGSRLF FWTM

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

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Macromolecule #12: Mitochondrial complex I, PDSW subunit

MacromoleculeName: Mitochondrial complex I, PDSW subunit / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.880752 KDa
SequenceString:
PDSWDKDVYP EPPRRTPAPS PQTSLPNPIT YLTKAFDLLV DRPVTLVREF IERQHAKNKY YYYHREFRRV PDITECHEKD VLCMFEAEM QWKRDYKVDQ EIVNIIQERL KACQQREGES HRQNCAKELQ QFTQVVKAYQ DRYHDLGAHY SARKCLAKQK Q RMLAERKA TKEAAAA

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Macromolecule #13: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 40.557148 KDa
SequenceString: MALRFLRLVP ASAASRGLAA VPPRVGGIHT SVQRKLQYGP LAYILGEKTT KKMTENSKLI TVDGNICSGK (SEP)KLAKE VAE KLGLKHFPEA GIHYADSTTG DGKPLPVRFS GNCSLEKFYD DPKSNDGNSY RLQAWLYASR LLQYADALEH LLSTGQG VV LERSIYSDFV ...String:
MALRFLRLVP ASAASRGLAA VPPRVGGIHT SVQRKLQYGP LAYILGEKTT KKMTENSKLI TVDGNICSGK (SEP)KLAKE VAE KLGLKHFPEA GIHYADSTTG DGKPLPVRFS GNCSLEKFYD DPKSNDGNSY RLQAWLYASR LLQYADALEH LLSTGQG VV LERSIYSDFV FLEAMYRQGF IRKQCVDHYN QVKKVTVCEY LPPHVVIYVD VPVSEVQSRI QKKGNPHEMK ITSAYLQD I ENVYKGTFLP EMSEKCEVLQ YSAWEAEDAE KVVEDIQYLK YDKGPWLDQD DRKLHNLRML VQDKLEVLNY TSIPVFLPE VTIGAHQSDR VFQEFTELPG RKYRAGYNED VGDKWIWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

+
Macromolecule #14: NADH:ubiquinone oxidoreductase subunit S5

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit S5 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 12.605594 KDa
SequenceString:
MPFFDVQKKL GVDLDHWMTI QSAEQPHRIP ARCHAFEKEW IECAHGIGSI RAEKECKIEF EDFRECLLRQ KTMKRLNAIK RQRDKLIKE GKYTPPPHHS GQEDLRP

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

+
Macromolecule #15: NADH:ubiquinone oxidoreductase subunit A3

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit A3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 9.325851 KDa
SequenceString:
MAPRVAAFLK NVWAKEPVLV ASFAIAGLAV ILPTLSPYTK YSLMINRATP YNYPVPLRDD GNMPDVPSHP QDPQGPSLEW LKRL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

+
Macromolecule #16: NADH:ubiquinone oxidoreductase subunit B3

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B3 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 11.146679 KDa
SequenceString:
MAHGHGHEHG PSKMELPDYK QWKIEGTPLE TVQEKLAARG LRDPWGRNEA WRYMGGFANN VSFVGALLKG FKWGFAAFVV AVGAEYYLE SQKKDKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

+
Macromolecule #17: NADH dehydrogenase [ubiquinone] 1 subunit C2

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.431655 KDa
SequenceString:
VTMMTGRQAR APLQFLPDEA RSLPPPKLTD PRLAYIGFLG YCSGLIDNAI RRRPVLSAGL HRQFLYITSF VFVGYYLLKR QDYMYAVRD HDMFSYIKSH PEDFPEKDKK TYREVFEEFH PVR

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

+
Macromolecule #18: NADH:ubiquinone oxidoreductase subunit B4

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B4 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 15.236375 KDa
SequenceString:
KMSFPKYEPS RLASLPTTLD PAEYDISSET RKAQAERLAI RSRLKREYQL QYNDPSRRGV VEDPALIRWT CARSANVYPN FRPNTKTSL LGALFGIGPL IFWYYVFKTD RDRKEKLIQE GKLDRTFNIS Y

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

+
Macromolecule #19: Mitochondrial complex I, B16.6 subunit

MacromoleculeName: Mitochondrial complex I, B16.6 subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 16.766461 KDa
SequenceString:
MAASKVKQDM PPVGGYGPID YKRNLPRRGL SGYSMFAVGI GALLFGYWSM MRWNRERRRL QIEDFEARIA LMPLLQAEKD RRVLQMLRE NLEEEATIMK DVPGWKVGES VFHTTRWVTP MMGELYGLRT GEEILSSTYG FIWYT

+
Macromolecule #20: Mitochondrial complex I, B17 subunit

MacromoleculeName: Mitochondrial complex I, B17 subunit / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 15.565126 KDa
SequenceString:
MSGYTPDEKL RLQQLRELRR RWLKDQELSP REPVLPPRRV SPVERFWNKF LQDGALWKNV IYKTYRHSIF AFTHVLIPVW IIHYYLKYH VTTKPYTIVE KKPRIFPGDT ILETGEVIPP MKEFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

+
Macromolecule #21: NADH:ubiquinone oxidoreductase subunit B7

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B7 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 16.413807 KDa
SequenceString:
MGAHLARRYL GDASVEPEPL RMPTFPPDYG FPERKEREMV ATQQEMNDAQ LVLQQRDYCA HYLIRFLKCK RDSFPNFLAC KHEQHDWDY CEHLDYVKRM KEFERERRLL QRKKRREQRE ADMAKGLGPG EVAPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

+
Macromolecule #22: NADH:ubiquinone oxidoreductase subunit B9

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 21.77991 KDa
SequenceString:
MAFLPSAAYL THQQKVLQLY KRALRHLESR CVHRDKYRYF ACLLRARFDE HKNEKDMVKA TQLLREAEKE FWHGQHPQPY IFPESPGGT SYERYECYKV PEWCLDDWHP SEKAMYPDYF AKREQWKKLR RESWEREVKQ LQEETPVGGP RTEALPPARK Q GDLPPLWW HIVTRPRERP M

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

+
Macromolecule #23: NADH:ubiquinone oxidoreductase subunit B2

MacromoleculeName: NADH:ubiquinone oxidoreductase subunit B2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 12.22677 KDa
SequenceString:
MAGMSALKRL APFAHVGGHL FRGRCARAVG AGGVRRAGGG AHIEPRYRQF PQLTRSQVIQ AEFFSATMWF WILWRFWHDS DAVLGHFPY PDPSQWTDEE LGIPPDDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

+
Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 21.752602 KDa
SequenceString:
MAAARAGVLG IRWLQKAARN VVPLGARTAS HITKDMLPGP YPKTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPKLPD RSQQERDPW YDWDHPDLRL NWGEPMHWDL DMYIRNRVDT SPTPVNWNLM CKHLFGFVAF MLFMFWVGET YPTYQPVGPK Q YPYNNLYL ERGGDPNKEP EPVVHYEI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

+
Macromolecule #25: Mitochondrial complex I, ESSS subunit

MacromoleculeName: Mitochondrial complex I, ESSS subunit / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 17.41066 KDa
SequenceString:
MAAGMLGLCG RRLLAVAATR GLPAASVRWE SSSSRAVIAP STLAGKRPSE PTLRWQEDPE PEDENLYEKN PDSHGYDKDP AVDVWNMRV VFFFGFSIVL VLGSTFVAYL PDYRMQEWAR REAERLVKYR EAHGLPLMES NCFDPSKIQL PEDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

+
Macromolecule #26: Mitochondrial complex I, KFYI subunit

MacromoleculeName: Mitochondrial complex I, KFYI subunit / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 8.802174 KDa
SequenceString:
MAPSAFLRPF WKLLAPARFP SVSSSRSKFY IQEPPHGSPN WLKVGLTLGT SAFLWIYLIK QHNEDVLEYK RRNGLE

+
Macromolecule #27: Mitochondrial complex I, MNLL subunit

MacromoleculeName: Mitochondrial complex I, MNLL subunit / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 7.075305 KDa
SequenceString:
MMNLLQVVRD HWIHVLVPVG FVFGYYLDRK NDEKLAAFRN KSLLYKRELK PQEEVTWK

+
Macromolecule #28: Mitochondrial complex I, MWFE subunit

MacromoleculeName: Mitochondrial complex I, MWFE subunit / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 8.211519 KDa
SequenceString:
MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGREKRVAH YSYQWYLMER DRRVSGVNRY YVSKGLENID

+
Macromolecule #29: Mitochondrial complex I, ND4L subunit

MacromoleculeName: Mitochondrial complex I, ND4L subunit / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 52.63441 KDa
SequenceString: MAALRALRRL RGAAAQVLRP GAGARLPIQP SRGARQWQPD VEWAEQYGGA VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHP AAHGVLRLVM ELSGEMVRKC DPHIGLLHRG TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSLAVEKLL N IQPPPRAQ ...String:
MAALRALRRL RGAAAQVLRP GAGARLPIQP SRGARQWQPD VEWAEQYGGA VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHP AAHGVLRLVM ELSGEMVRKC DPHIGLLHRG TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSLAVEKLL N IQPPPRAQ WIRVLFGEIT RLLNHIMAVT THALDIGAMT PFFWMFEERE KMFEFYERVS GARMHAAYVR PGGVHQDLPL GL MDDIYEF SKNFSLRIDE LEEMLTNNRI WRNRTVDIGV VTAEDALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEFD VPI GSRGDC YDRYLCRVEE MRQSIRIISQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAI EAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEVDR

+
Macromolecule #30: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 30 / Number of copies: 5 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

+
Macromolecule #31: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione

MacromoleculeName: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione
type: ligand / ID: 31 / Number of copies: 1 / Formula: DCQ
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 322.439 Da
Chemical component information

ChemComp-DCQ:
2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione

+
Macromolecule #32: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 32 / Number of copies: 12 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

+
Macromolecule #33: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 33 / Number of copies: 7 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

+
Macromolecule #34: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 34 / Number of copies: 1 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

+
Macromolecule #35: ADENOSINE MONOPHOSPHATE

MacromoleculeName: ADENOSINE MONOPHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: AMP
Molecular weightTheoretical: 347.221 Da
Chemical component information

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

+
Macromolecule #36: MYRISTIC ACID

MacromoleculeName: MYRISTIC ACID / type: ligand / ID: 36 / Number of copies: 1 / Formula: MYR
Molecular weightTheoretical: 228.371 Da
Chemical component information

ChemComp-MYR:
MYRISTIC ACID

+
Macromolecule #37: water

MacromoleculeName: water / type: ligand / ID: 37 / Number of copies: 1293 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration3 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R0.6/1 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 100.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5lnk

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 315484
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5lnk


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT / Overall B value: 50 / Target criteria: Correlation coefficient
Output model

PDB-6zka:
Membrane domain of open complex I during turnover

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