EMDB-10779: 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet ...

Basic information

• Entry: Database: EMDB / ID: EMD-10779
• Title: 55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST)

Sample

• Complex: 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA
  • Complex: 55S S. scrofa mitochondrial ribosome
    • Protein or peptide: x 83 types
    • RNA: x 2 types
  • Complex: tRNAMet and mRNA
    • RNA: x 2 types
  • Complex: Elongation factor G, mitochondrialEF-G
• Ligand: x 8 types

Function / homology

Function:

Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / : / mitochondrial translational termination / mitochondrial transcription / mitochondrial ribosome assembly / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex / Mitochondrial translation elongation / ribonuclease III activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / mitochondrial small ribosomal subunit / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / mitochondrial translation / positive regulation of proteolysis / ribosomal small subunit binding / organelle membrane / RNA processing / translation elongation factor activity / rescue of stalled ribosome / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / double-stranded RNA binding / large ribosomal subunit / regulation of translation / cell junction / small ribosomal subunit / nuclear membrane / negative regulation of translation / rRNA binding / nuclear body / ribosome / mitochondrial matrix / structural constituent of ribosome / cell cycle / ribonucleoprotein complex / translation / protein domain specific binding / nucleotide binding / mRNA binding / intracellular membrane-bounded organelle / GTPase activity / synapse / apoptotic process / GTP binding / nucleolus / positive regulation of DNA-templated transcription / mitochondrion / extracellular space / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm

Domain/homology:

Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / : / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / 40S ribosomal protein rpS2 (S5p), N-terminal / 28S ribosomal protein S25, mitochondrial / Translation elongation factor EFG/EF2 / Elongation factor G, domain III / EFG, domain V / Pentatricopeptide repeat domain / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L55 / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein S24/S35, mitochondrial, conserved domain / Ribosomal protein S30, mitochondrial / Mitochondrial ribosomal subunit protein / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein L37/S30 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Mitochondrial 28S ribosomal protein S30 (PDCD9) / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / Ribosomal protein L35, mitochondrial / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / MRPL44, double-stranded RNA binding domain / Tim44-like domain / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Tim44-like domain / Ribosomal protein L7/L12 dimerisation domain / Tim44 / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Ribosomal protein L49/IMG2 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial large subunit ribosomal protein (Img2) / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic

Component:

Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / Large ribosomal subunit protein mL62 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m / Ribosomal protein / Mitochondrial ribosomal protein S23 / 28S ribosomal protein S24, mitochondrial / Mitochondrial ribosomal protein S22 / Mitochondrial ribosomal protein L12 / Mitochondrial ribosomal protein S34 / Mitochondrial ribosomal protein L41 / Progonadoliberin / Small ribosomal subunit protein mS38 / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein mL37 / Small ribosomal subunit protein uS15m / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / Large ribosomal subunit protein mL53 / 39S ribosomal protein L48, mitochondrial / Small ribosomal subunit protein bS16m / 39S ribosomal protein S30, mitochondrial / Mitochondrial ribosomal protein S27 / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Mitochondrial ribosomal protein S17 / Small ribosomal subunit protein mS31 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL22m / Mitochondrial ribosomal protein S18A / Mitochondrial ribosomal protein L2 / 39S ribosomal protein L15, mitochondrial / 39S ribosomal protein L27, mitochondrial / Large ribosomal subunit protein uL11m / Small ribosomal subunit protein uS10m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / 28S ribosomal protein S2, mitochondrial / Small ribosomal subunit protein mS33 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / 28S ribosomal protein S35, mitochondrial isoform 1 / Large ribosomal subunit protein uL29m / Mitochondrial ribosomal protein L39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein uL30m / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein uS5m / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein mL50 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein S6 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL9m / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein bL32m / 28S ribosomal protein S9, mitochondrial / Small ribosomal subunit protein mS39 / 39S ribosomal protein L13, mitochondrial / Small ribosomal subunit protein mS40 / Elongation factor G, mitochondrial

• Biological species: Sus scrofa (pig) / Homo sapiens (human) / Pig (pig)
• Method: single particle reconstruction / cryo EM / Resolution: 4.2 Å
• Authors: Kummer E / Ban N

Funding support

Switzerland, 3 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	1196-2014	Switzerland
Swiss National Science Foundation	138262	Switzerland
Swiss National Science Foundation	310030B_163478	Switzerland

• Citation: Journal: EMBO J / Year: 2020; Title: Structural insights into mammalian mitochondrial translation elongation catalyzed by mtEFG1.; Authors: Eva Kummer / Nenad Ban / ; Abstract: Mitochondria are eukaryotic organelles of bacterial origin where respiration takes place to produce cellular chemical energy. These reactions are catalyzed by the respiratory chain complexes located in the inner mitochondrial membrane. Notably, key components of the respiratory chain complexes are encoded on the mitochondrial chromosome and their expression relies on a dedicated mitochondrial translation machinery. Defects in the mitochondrial gene expression machinery lead to a variety of diseases in humans mostly affecting tissues with high energy demand such as the nervous system, the heart, or the muscles. The mitochondrial translation system has substantially diverged from its bacterial ancestor, including alterations in the mitoribosomal architecture, multiple changes to the set of translation factors and striking reductions in otherwise conserved tRNA elements. Although a number of structures of mitochondrial ribosomes from different species have been determined, our mechanistic understanding of the mitochondrial translation cycle remains largely unexplored. Here, we present two cryo-EM reconstructions of human mitochondrial elongation factor G1 bound to the mammalian mitochondrial ribosome at two different steps of the tRNA translocation reaction during translation elongation. Our structures explain the mechanism of tRNA and mRNA translocation on the mitoribosome, the regulation of mtEFG1 activity by the ribosomal GTPase-associated center, and the basis of decreased susceptibility of mtEFG1 to the commonly used antibiotic fusidic acid.

History

Deposition	Mar 21, 2020	-
Header (metadata) release	Jul 15, 2020	-
Map release	Jul 15, 2020	-
Update	Aug 12, 2020	-
Current status	Aug 12, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_10779.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.045 / Movie #1: 0.045
Minimum - Maximum	-0.16235815 - 0.2894498
Average (Standard dev.)	0.0008609195 (±0.012041429)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 320 320 320 Spacing 320 320 320
Cell	A=B=C: 444.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	320	320	320
origin x/y/z	0.000	0.000	0.000
length x/y/z	444.800	444.800	444.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	320	320	320
D min/max/mean	-0.162	0.289	0.001

Supplemental data

Mask #1

• File: emd_10779_msk_1.map

Supplemental map: emd 10779 additional.map

• File: emd_10779_additional.map

Half map: #1

• File: emd_10779_half_map_1.map

Half map: #2

• File: emd_10779_half_map_2.map

Sample components

Entire : 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two ...

• Entire: Name: 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA

Components

• Complex: 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA
  • Complex: 55S S. scrofa mitochondrial ribosome
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L19
    • Protein or peptide: Mitochondrial ribosomal protein L20
    • Protein or peptide: Mitochondrial ribosomal protein L21
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L37
    • Protein or peptide: Mitochondrial ribosomal protein L38
    • Protein or peptide: Mitochondrial ribosomal protein L39
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L41
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L43
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L45
    • Protein or peptide: Mitochondrial ribosomal protein L46
    • Protein or peptide: 39S ribosomal protein L48, mitochondrialRibosome
    • Protein or peptide: Mrpl34
    • Protein or peptide: Mitochondrial ribosomal protein L50
    • Protein or peptide: Mitochondrial ribosomal protein L51
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: mL53
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L57
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L27
    • Protein or peptide: 39S ribosomal protein S30, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein S18A
    • RNA: 12S rRNAMT-RNR1
    • Protein or peptide: Mitochondrial ribosomal protein S2
    • Protein or peptide: Mitochondrial ribosomal protein S24
    • Protein or peptide: Mitochondrial ribosomal protein S5
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein S7
    • Protein or peptide: Mitochondrial ribosomal protein S10
    • Protein or peptide: uS11m
    • Protein or peptide: Mitochondrial ribosomal protein S12
    • Protein or peptide: Mitochondrial ribosomal protein S14
    • Protein or peptide: uS15m
    • Protein or peptide: 28S ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: uS17m
    • Protein or peptide: Mitochondrial ribosomal protein S18C
    • Protein or peptide: bS21m
    • Protein or peptide: unknown peptide
    • Protein or peptide: Mitochondrial ribosomal protein S22
    • Protein or peptide: mS23
    • Protein or peptide: Mitochondrial ribosomal protein S25
    • Protein or peptide: Mitochondrial ribosomal protein S26
    • Protein or peptide: Mitochondrial ribosomal protein S27
    • Protein or peptide: Mitochondrial ribosomal protein L28
    • Protein or peptide: Mitoribosomal protein ms28, mrps28
    • Protein or peptide: Death associated protein 3
    • Protein or peptide: Mitochondrial ribosomal protein S31
    • Protein or peptide: mS33
    • Protein or peptide: mS34
    • Protein or peptide: Mitochondrial ribosomal protein S35
    • Protein or peptide: mS37
    • Protein or peptide: Aurora kinase A interacting protein 1
    • Protein or peptide: mS39
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L12
    • Protein or peptide: Mitochondrial ribosomal protein L47
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L34
    • Protein or peptide: Mitochondrial ribosomal protein L35
    • Protein or peptide: Ribosomal protein
    • RNA: CP tRNA-Phe
    • Protein or peptide: Elongation factor G, mitochondrialEF-G
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: ICT1
    • Protein or peptide: Mitochondrial ribosomal protein L4
    • Protein or peptide: Mitochondrial ribosomal protein L9
    • Protein or peptide: Mitochondrial ribosomal protein L10
    • Protein or peptide: Mitochondrial ribosomal protein L11
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Mitochondrial ribosomal protein L18
  • Complex: tRNAMet and mRNA
    • RNA: tRNAMet
    • RNA: mRNAMessenger RNA
  • Complex: Elongation factor G, mitochondrialEF-G
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: SPERMINE
• Ligand: N-FORMYLMETHIONINE
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: GUANOSINE-5'-MONOPHOSPHATE
• Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
• Ligand: water

Supramolecule #1: 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two ...

• Supramolecule: Name: 55S S. scrofa mitochondrial ribosome in complex with mtEFG1, two tRNA-Met and CUGAUG mRNA; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#87
• Molecular weight: Experimental: 2.8 MDa

Supramolecule #2: 55S S. scrofa mitochondrial ribosome

• Supramolecule: Name: 55S S. scrofa mitochondrial ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#45, #48-#87
• Source (natural): Organism: Sus scrofa (pig)

Supramolecule #3: tRNAMet and mRNA

• Supramolecule: Name: tRNAMet and mRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #46-#47
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Synthetic construct (others)

Supramolecule #4: Elongation factor G, mitochondrial

• Supramolecule: Name: Elongation factor G, mitochondrial / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #75
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Macromolecule #1: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 1 / Details: residues 187-194 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 45.60457 KDa

Sequence

String:

MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK LLDVEKHHNQ LRAKDLLAEK TKILKDPIGS RWLIKEELEE MLVEKLSDQD YAQFIRLLER LSALPCGATE ED FVNRFRR SIPIQSKKQL IEPLQYDEQG MAFSRGEGKR KTAKAEVVVY GQGSGRIDVN GVDYLLYFPV TQDREQLMFP LHF LDRLGK HDMTCAVSGG GRSAQAGAVR LAMARALCSF VTEDEVEWMR QAGLLTADPR VRERKKPGQE GARRKFTWKK R

Macromolecule #2: Mitochondrial ribosomal protein L19

• Macromolecule: Name: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.441539 KDa

Sequence

String:

MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

Macromolecule #3: Mitochondrial ribosomal protein L20

• Macromolecule: Name: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 17.554793 KDa

Sequence

String:

MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR

Macromolecule #4: Mitochondrial ribosomal protein L21

• Macromolecule: Name: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.154861 KDa

Sequence

String:

MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C

Macromolecule #5: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.313357 KDa

Sequence

String:

MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL

Macromolecule #6: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 6 / Details: residues 115-133 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 17.643205 KDa

Sequence

String:

MARNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGSNRRR DHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L

Macromolecule #7: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.893529 KDa

Sequence

String:

MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY

Macromolecule #8: Mitochondrial ribosomal protein L37

• Macromolecule: Name: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 47.917988 KDa

Sequence

String:

MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV

Macromolecule #9: Mitochondrial ribosomal protein L38

• Macromolecule: Name: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 44.656387 KDa

Sequence

String:

MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

Macromolecule #10: Mitochondrial ribosomal protein L39

• Macromolecule: Name: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 38.523422 KDa

Sequence

String:

MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST

Macromolecule #11: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.075658 KDa

Sequence

String:

MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL FPFERQGPDY TPPISDYQPP EGRYQDITKV YTQVEFKK

Macromolecule #12: Mitochondrial ribosomal protein L41

• Macromolecule: Name: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.073404 KDa

Sequence

String:

MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR

Macromolecule #13: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 13 / Details: residues 77-100 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 16.680178 KDa

Sequence

String:

MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR

Macromolecule #14: Mitochondrial ribosomal protein L43

• Macromolecule: Name: Mitochondrial ribosomal protein L43 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 17.728109 KDa

Sequence

String:

MTARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVVAEYL NGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQVQAQ

Macromolecule #15: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 37.527195 KDa

Sequence

String:

MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS

Macromolecule #16: Mitochondrial ribosomal protein L45

• Macromolecule: Name: Mitochondrial ribosomal protein L45 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 35.331805 KDa

Sequence

String:

MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA

Macromolecule #17: Mitochondrial ribosomal protein L46

• Macromolecule: Name: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 32.215998 KDa

Sequence

String:

MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL

Macromolecule #18: 39S ribosomal protein L48, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L48, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 29.942523 KDa

Sequence

String:

MSEAAGTKVL RELNASRSDI VTGPDQGRSV SIFRPPGIAA AWADLQLARL HTTRQQRMNG ALGKALCLRN DTVLKQALSL IRVRASGES PICSAGGILL STSRHYRSKP THGIGRYKHL VKAQEPKKKK GKVEVRPINL GTDYEYGVLN IHLIAYDMAL A ESYAQYVH NLCNHLAIKV EESYAMPTKT MEVLQLQEQG SKMFLDAVLT THERVVQISG LSATFAEIFL EIIHSNLPEG VK LSVREHT EEDFKGRFKA RPELEELLAK LN

Macromolecule #19: Mrpl34

• Macromolecule: Name: Mrpl34 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.957779 KDa

Sequence

String:

MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF

Macromolecule #20: Mitochondrial ribosomal protein L50

• Macromolecule: Name: Mitochondrial ribosomal protein L50 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 22.785357 KDa

Sequence

String:

MVRLRGKREC LTLSLRLIGW SWRVTAGGGR CFVSKFSSKM AARWVSGLAR RSLTCAVSGA PRREFWSPFR KEKQPVVAET VEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQDI SLEDVHLKFS FLARLANDLR HAVPNSRLHQ M CRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY

Macromolecule #21: Mitochondrial ribosomal protein L51

• Macromolecule: Name: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.249048 KDa

Sequence

String:

MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR

Macromolecule #22: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 13.813705 KDa

Sequence

String:

MAAWGILLST GVRRLHCGTA AQAGSQWRLQ QGFAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAQR EKFARRVVLL SQEMDAGLQ AWQLRQQEKL QEEEGKQKNA LKSKGALLQN PQPSQ

Macromolecule #23: mL53

• Macromolecule: Name: mL53 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 12.015747 KDa

Sequence

String:

MAAALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIMRGAH LTAQEMLTA FASHIQARGA AGSGDKPSAS TGR

Macromolecule #24: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 24 / Details: residues 54-64 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.957357 KDa

Sequence

String:

MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF

Macromolecule #25: Mitochondrial ribosomal protein L57

• Macromolecule: Name: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 12.001915 KDa

Sequence

String:

MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS

Macromolecule #26: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 26 / Details: residues 165-173 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.545873 KDa

Sequence

String:

MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD

Macromolecule #27: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 25.815031 KDa

Sequence

String:

MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS

Macromolecule #28: Mitochondrial ribosomal protein L27

• Macromolecule: Name: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.943688 KDa

Sequence

String:

MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML

Macromolecule #29: 39S ribosomal protein S30, mitochondrial

• Macromolecule: Name: 39S ribosomal protein S30, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 49.100504 KDa

Sequence

String:

MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

Macromolecule #30: Mitochondrial ribosomal protein S18A

• Macromolecule: Name: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 22.273084 KDa

Sequence

String:

MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH

Macromolecule #32: Mitochondrial ribosomal protein S2

• Macromolecule: Name: Mitochondrial ribosomal protein S2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 31.731604 KDa

Sequence

String:

MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR YFKGGLLTNA PLLLGPGVRL PDLIIFLHTL NNVFEPHVAV RDAAKMNIPT VGIVDTNCNP ALITYPVPGN DD SPPAVRL FCRLFQVAIS RAKEKRRQVE ALYRLQGPAG AERGPAAPPP PE

Macromolecule #33: Mitochondrial ribosomal protein S24

• Macromolecule: Name: Mitochondrial ribosomal protein S24 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.935172 KDa

Sequence

String:

MVASVCGGGL RPLVLSWSRE LPCAWRALHI SAVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMLGT FPGCLADQLV LKRRANQLEI CALVLRQLPP HKFYFLVGYS ETLLSHFYKC PVHLHLQTVP S KVVYKYI

Macromolecule #34: Mitochondrial ribosomal protein S5

• Macromolecule: Name: Mitochondrial ribosomal protein S5 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 48.213074 KDa

Sequence

String:

MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV EADMVQQREE WDRRRKMKVK RERGWSGNTW GGVSLGPPDP GPNGETYDDF DTRILEVRNV FNMTAKEGRK RS VRVLVAV GNGKGAAGFA IGKATERADA FRKAKNRAVH YLHYIERYED HTIYHDISLK FKRTHIKMKK QPRGYGLHCH RAI MTICRL IGIKDLYAKV SGSVNMLNLT RGLFLGLSRQ ETHQQLADKK SLHVVEFREE CGPLPIVVAS PQGALRKDPE PEDE VPDIT LDWEDVKAAQ GMKRSVWSGL KRAAT

Macromolecule #35: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 30.365266 KDa

Sequence

String:

MRRKAWKVPA GSRTIRSKRG GSGSCRVGGV CSSRWQLAEG TRGAALLQWL PGQGAGPRAG CRPQRAGLSR GRGAAALRRG RGVARGGAW LGEGAWRPVC AAARAFKPGL ARPERAFAAW EPSGPAVRQV SAVPALFHPG RRFREEPRAV HSGMPRYELA L ILKAMQRP ETAAALKRTL EALMDRGAVV RNLENLGERM LPYKISAHNQ RHSRGGYFLV DFYAPATTVE SMMEHLSRDI DV IRPNIVK HPLTQEVKEC EGIVPVPLEE KLYSTKKRK

Macromolecule #36: Mitochondrial ribosomal protein S7

• Macromolecule: Name: Mitochondrial ribosomal protein S7 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 28.150918 KDa

Sequence

String:

MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY QVPVPLADRR RRFLAMKWMI AECREKKHRR VLMPEKLSQE LLEAFHNQGP VIKRKHDMHK MAEANRALAH YR WW

Macromolecule #37: Mitochondrial ribosomal protein S10

• Macromolecule: Name: Mitochondrial ribosomal protein S10 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.105678 KDa

Sequence

String:

MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLTT MKWVQFSNLH VDVPKDMTKP EITISDEPDT LYKRLSVLVK GHDKAVLDS YEYFAVLAAK ELGISIKVHE PPRKIERFTL LKSVHIFKKH RVQYEMRTLY RCLELEHLTG STADVYLEYI Q RNLPEGVA MEVTKTKLEQ LPEHIRKPIW ETMPEEKEES KS

Macromolecule #38: uS11m

• Macromolecule: Name: uS11m / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.841922 KDa

Sequence

String:

MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT MGGLEVISIT DNTPIPHNGC RPRKARRL

Macromolecule #39: Mitochondrial ribosomal protein S12

• Macromolecule: Name: Mitochondrial ribosomal protein S12 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.257107 KDa

Sequence

String:

MSWSGLLRGL GTSLNCGLAL VPRPWATRPM ATLNQMHRLG PPKHPPGKMG PTAGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HSLQEHHVVL VQGGRTQDLP GVKLTVVRGK YDCGHVQKKK

Macromolecule #40: Mitochondrial ribosomal protein S14

• Macromolecule: Name: Mitochondrial ribosomal protein S14 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.120739 KDa

Sequence

String:

MAASMLGFLL RTVRQMVPSS ASGQVRSYYV DWKMLRDVKR RKMAYEYADE RLRINSLRKN TILPKDLQEV ADEEIASLPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGVQRAMW

Macromolecule #41: uS15m

• Macromolecule: Name: uS15m / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 27.411428 KDa

Sequence

String:

MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP VQPNQDDPPP SMLLLDYQNV PGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN YEEHMQKHRK DKAHKRFLLM S IDQRKKML KNLRETNYAV FEKICKELGI EYTFPPPYHR KAHRRWVTKK ALCTQVFREV QKLKKQKRAL RAAAVAAHKQ G

Macromolecule #42: 28S ribosomal protein S16, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.182668 KDa

Sequence

String:

MVQLTTVLCR AYRGGHLTIR LALGGCTNRP FYRIVAAHSK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNF DRIRHWIGCG AHLSKPVEK LLGLSGFYPL HPMMITNAER LRRKRAREVL LASQKTDTEA TETKAS

Macromolecule #43: uS17m

• Macromolecule: Name: uS17m / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 14.452014 KDa

Sequence

String:

MSIARSSVHA KWVVGKVIGT AMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLKALP VPRTKHVKHE LAEIIFKVG QVIDPVTGKP CAGTTYLESP ISPESTRLTK NLEALQLSST Q

Macromolecule #44: Mitochondrial ribosomal protein S18C

• Macromolecule: Name: Mitochondrial ribosomal protein S18C / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 16.211086 KDa

Sequence

String:

MAAVVSLCSR LGRRKSTDFV NAAVCLTDPG THAVLWRSCS QYKQVASNED LPVPMENPYK EPLKKCILCE KHVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQKEIT KAIKRAQILG FMPVTYKDPA YLKDPKVCNI KYRE

Macromolecule #45: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 10.682506 KDa

Sequence

String:

MANHLKFIAR TVMVQEGNVE GAYRTLNRIL TMDGLIEDIK RRRYYEKPCR RRQRESYETC RRIYNMEMAR KINFLMRKNR ADPWQGC

Macromolecule #48: unknown peptide

• Macromolecule: Name: unknown peptide / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 1.297416 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAA

Macromolecule #49: Mitochondrial ribosomal protein S22

• Macromolecule: Name: Mitochondrial ribosomal protein S22 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 43.234645 KDa

Sequence

String:

MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ILEGTETAKY VFTDISYSIP HRERFIVVRE PSGTLRKASW EERDRMIQVY FPREGRRILT PVIFKEENLQ TM YSQDQHV DVLNLCVAQF EPDSAEYIKI HHHTYEDIDK CGKYDLLRST RHFGGMAWYF VNKKKIDGLL IDQIQRDLVS DAT SLVHLY HILHPDGQSA QEAKKQGAEG LHLIKVFAKT EAQKGAYIEL TLQAYQEAFI THSAAS

Macromolecule #50: mS23

• Macromolecule: Name: mS23 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.666674 KDa

Sequence

String:

MAGSRLETVG SIFSRTRDLI RAGVLKEKPL WLDIYNAFPP LREPVFRRPR LRYGKAKAAV QDIFYHEDRI RAKFYSAYGS GPKAFDLFN PNFKSTCQRF VEKYIELQRL GETDEEKLFV EAGKALLAEG VTLRRVGEAR TQQEGSPISR KSEPTGIKPQ T AVEENQPW KEVPQDQPLE APEKESKGLP PP

Macromolecule #51: Mitochondrial ribosomal protein S25

• Macromolecule: Name: Mitochondrial ribosomal protein S25 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 19.954186 KDa

Sequence

String:

MPMKGRFPIR RTLQYLGQGD VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQITMFKNM TPSPFLRFYL DSGEQVLVD VESKSNKEIV EHIRKILGKN EETLEKEEQE KKQLSHPAHF GPRKYCLREC MCEVEGQVPC PGLVPLPKEM T GKYKAALK ATAQD

Macromolecule #52: Mitochondrial ribosomal protein S26

• Macromolecule: Name: Mitochondrial ribosomal protein S26 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.6379 KDa

Sequence

String:

MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI TRENLEARVE EALDSPKSYN WAITREGLVV RPQQKGS

Macromolecule #53: Mitochondrial ribosomal protein S27

• Macromolecule: Name: Mitochondrial ribosomal protein S27 / type: protein_or_peptide / ID: 53 / Details: residues 337-352 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 51.945984 KDa

Sequence

String:

MLSRAAPTEG TGGGRRSVDS TPPPRLPATP APAFPLVCSK MASSIVRRGI LRARKVSLPQ LSLAGRRCLL SAAYVDSHKW EARDKEDCH LADLASLMDK TYERKLPVSS LTISRFVDNI ASREDIDHAE YYLYKFRHSP NCWYLRDWTI HTWIRQCLKY G AQDKALYT LKNKVQYGIF PDNYTFNLLM DYFIKKENYK DALSVVFEIM MQEAFEVPST QLLSLYVLYH CLAEKTDLSW EE ERNFGAS LLLPGLKQKN SVGLSSQLYG YALLGKVELQ QGLRAVYCNM PLLWQPGYLH RALQVMEKVA SSPEDGQLCG EAL DVLDRV LKAVTAPAQG TSEEQPQEGE ESQRSEELVE QLDVEETEQS KLPQYLERFE ALRSKLQALG KVESESLLTL STQL AKAQL PTCEAEDIAV YEQRLQQWHG ERVRLIQREQ EQRERARQDY EARRAAKASA

Macromolecule #54: Mitochondrial ribosomal protein L28

• Macromolecule: Name: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 30.148953 KDa

Sequence

String:

MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK

Macromolecule #55: Mitoribosomal protein ms28, mrps28

• Macromolecule: Name: Mitoribosomal protein ms28, mrps28 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.014912 KDa

Sequence

String:

MAALCRTRAV TAKSHFLRVF FFSRPCRGTG TESGSGSESS ESTEPKQRPG GFASALERHS ELQQKAEFGR MRGSPKNVES FASMLRHSP LTQMGPAKNK LVIGQIFHIV EDDLYIDFGG KFHCVCKRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT D TTILEADA VLLGLQESKD SKSKEERHEK

Macromolecule #56: Death associated protein 3

• Macromolecule: Name: Death associated protein 3 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 46.966062 KDa

Sequence

String:

MAQLLANLTS IQVMLKGVTR LISRVHKLDP GHVLYMGTQA PQSLAAHLDN QVPVKSPRAI SRTSEDDPAK HREQHEGQHY NISLQELKT VFPHGLPPRF AMQVKTFNEA CLMVRKPALE LLHYLKNTNF AHPAVRYVLY GEKGTGKTLS LCHILHFCAK Q NWLILHIP DAHIWVKNCR DLLQSNYNKQ RFDQPLEAST WLKNFKTANE HFLSQIKVQE KYVWNKREST EKGRPLGEVV EQ GIMRVRN ATDAVGIVLK ELKRQSSLGI FHLLVAVDGV NALWGRTTLK REDKSPIAPE ELALIHNLRK MVKNDWQGGA IVL TVSQTG SLFKPRNAYL PQELLGKEGF DALDPFIPIL VSNYNPKEFE SCIQYYLENN WLQHEKAHTE EGKKELLFLS NRNP GQLER LCAYL

Macromolecule #57: Mitochondrial ribosomal protein S31

• Macromolecule: Name: Mitochondrial ribosomal protein S31 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 44.091996 KDa

Sequence

String:

MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL RQLQKHEEDS R(UNK)QKDGEKPK ISFSNIISDM KVARSATARV STRPVHQIQF DEGTDDFADQ EKTLNLKK S FRKNIFKGRR LNIFEPQAVT EEAPETEAVP SLWDIEFAKQ LAAVTERPFQ NGFEEMIQWT KEGKLWEFPI NNEAGFDDD GSEFHEHIFL DKYLQDFPKQ GPIRHFMELV TCGLSKNPYL SVKQKVEHIE WFRNYFNEKR VILKESGIQL N

Macromolecule #58: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 12.54465 KDa

Sequence

String:

MSSLSEYAVR MSRLSARLFG EVARPTDSKS MKVVKLFSEQ PLAKRKETYD WYPNHNTYFA LMGTLRFLGL YRDEHQDFRD EQLRLKKLR GKGKPRKGEG KRAMKKK

Macromolecule #59: mS34

• Macromolecule: Name: mS34 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 25.843818 KDa

Sequence

String:

MARKKVRPRL IAELARRVRA LREQRERPRD SQRYALDYET MMRPHSGNRL PMRAWADVRH ESRLLQLLNR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYAAENL DHGKAWGILT FKGKTESEAR EIEQVMYHDW RLVPKHEEEA FTAFTLVPED T PLSVPYPP LLRAMILAQR QKYGDTSTEE PMLNLERARI DPWDYPAKLE AKTKTKGAAV

Macromolecule #60: Mitochondrial ribosomal protein S35

• Macromolecule: Name: Mitochondrial ribosomal protein S35 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 37.104809 KDa

Sequence

String:

MAATSLPAWL ILKSRAGTFR AFSTAMSPAT RSPRPALRTT ERTSKPERAL RRKALPPRTE KMAVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKRHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYV S AGPSIRNP KARVVTLRVK LSSLNLDDHA KKKLIKLVGD RYCKSTDVLT IKTDRCPLKR QNYDYAVYLL TVLYHESWKT EE WEKKKTE ADMEEYIWEN STSEKNILET LLQIKAAEKN LELSKEELLG TKEVEDYRKS VVSLKNEGDN ENTLSQYKES VKR LLNLA

Macromolecule #61: mS37

• Macromolecule: Name: mS37 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 13.561903 KDa

Sequence

String:

MATPSLRGRL ARLGNPRRPI LKPNKPLILA NHVGERRREK GEATCITEMS VMMACWKQNE FRDEACRKEI QDFFDCASRA EAARKMRSI QENLGELGSL PPKKLNKLLH RFPNKPHVS

Macromolecule #62: Aurora kinase A interacting protein 1

• Macromolecule: Name: Aurora kinase A interacting protein 1 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 22.843941 KDa

Sequence

String:

MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK FERDLKRIWQ KAGLKEAPAG WQTPKIYLKN Q

Macromolecule #63: mS39

• Macromolecule: Name: mS39 / type: protein_or_peptide / ID: 63 / Details: residues 201-234 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 78.518156 KDa

Sequence

String:

MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKLRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GNQEPSANYN FQQREQSEEL EEATEADNEK SKTKAGHHLG VT WRTKNNA ERIFALMPEK NAHSYCTMIR GMVKHQAPTQ ALNLYTVLLN NRLRADVYTF NSLIEATALV VNEKFEEKWN NIL DLLKQM VTQNVKPNLQ TFNTILKCLR RFYAFGKLPA LQTLREMKAI GIEPSLATYH YVIQLFYQHE SPSKGSSLII YDIM NEVMG KRFSPRDPDD DMFFQSAMRV CSSLRDLELA YQVHGLLNTG DNWKLIGSDH RRNFYYSKFF NLLCFMEQID VTLKW YKDL IPSVFFPHSQ TMIDLLQALD VANRLDMVPQ IWKDSKEYGH TFRNELKEEI LMLMARDQHP PELQVAFADC AADIKS TYE SQDARQTAPE WPASSLNYVA VLFLRAGRTQ EAWKMLGLFR KHNKIPRAEL LNEFLDSAKA SSSPAQAIEL VKLASAF SL PVCEGLTRRV MAEFTLTQEQ REALGELTAL TSDSSSDSDS DSDSDISEGK

Macromolecule #64: 28S ribosomal protein S18b, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 29.220465 KDa

Sequence

String:

MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP QVEPRDLDFS TTHGAVSSTP PAPTLVSGDP WYPWYSWKQP PERELSRLRR LYQGRLREES GPPPELMPEV PL TAPAEAS STEPGAPQSA L

Macromolecule #65: Mitochondrial ribosomal protein L12

• Macromolecule: Name: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 65 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.268975 KDa

Sequence

String:

MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE

Macromolecule #66: Mitochondrial ribosomal protein L47

• Macromolecule: Name: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 30.085088 KDa

Sequence

String:

MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG AWRRDIFGRI IWHKFKQWPI PWYLNKKYNR KRFFAMPYVE RFVRMRIEKQ ARIKARKRSL ERKKEKFLQE KF PHLSETQ KSSHV

Macromolecule #67: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.9211 KDa

Sequence

String:

HPPRQDFLVR RQPHRNRTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S

Macromolecule #68: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 68 / Details: residues 83-96 built as poly-Ala / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.156537 KDa

Sequence

String:

MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

Macromolecule #69: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.53793 KDa

Sequence

String:

MASAMLVLVV PPWPAARGLL RNWWEQLQRK LQHNRLGLPL HPWGPALAVQ GPAICTEPAN DTNGSKAISS LLDSVFWMAA PKNRRSIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HILCGYCYEK VRKETAEIRR QMGKQEGGPF RAPTTETVVL Y SGETPSEQ DQGKRIIERE RKRPSWFTQN

Macromolecule #70: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 7.508097 KDa

Sequence

String:

MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL

Macromolecule #71: Mitochondrial ribosomal protein L34

• Macromolecule: Name: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 10.871729 KDa

Sequence

String:

MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH

Macromolecule #72: Mitochondrial ribosomal protein L35

• Macromolecule: Name: Mitochondrial ribosomal protein L35 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.548543 KDa

Sequence

String:

MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV

Macromolecule #73: Ribosomal protein

• Macromolecule: Name: Ribosomal protein / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 10.841016 KDa

Sequence

String:

MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M

Macromolecule #75: Elongation factor G, mitochondrial

• Macromolecule: Name: Elongation factor G, mitochondrial / type: protein_or_peptide / ID: 75 ; Details: mtEFG1 residues 40-751 including an N-terminal SGGSGSGSSG-linker; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 80.054148 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

GGSGSGSSGV IPNEKIRNIG ISAHIDSGKT TLTERVLYYT GRIAKMHEVK GKDGVGAVMD SMELERQRGI TIQSAATYTM WKDVNINII DTPGHVDFTI EVERALRVLD GAVLVLCAVG GVQCQTMTVN RQMKRYNVPF LTFINKLDRM GSNPARALQQ M RSKLNHNA AFMQIPMGLE GNFKGIVDLI EERAIYFDGD FGQIVRYGEI PAELRAAATD HRQELIECVA NSDEQLGEMF LE EKIPSIS DLKLAIRRAT LKRSFTPVFL GSALKNKGVQ PLLDAVLEYL PNPSEVQNYA ILNKEDDSKE KTKILMNSSR DNS HPFVGL AFKLEVGRFG QLTYVRSYQG ELKKGDTIYN TRTRKKVRLQ RLARMHADMM EDVEEVYAGD ICALFGIDCA SGDT FTDKA NSGLSMESIH VPDPVISIAM KPSNKNDLEK FSKGIGRFTR EDPTFKVYFD TENKETVISG MGELHLEIYA QRLER EYGC PCITGKPKVA FRETITAPVP FDFTHKKQSG GAGQYGKVIG VLEPLDPEDY TKLEFSDETF GSNIPKQFVP AVEKGF LDA CEKGPLSGHK LSGLRFVLQD GAHHMVDSNE ISFIRAGEGA LKQALANATL CILEPIMAVE VVAPNEFQGQ VIAGINR RH GVITGQDGVE DYFTLYADVP LNDMFGYSTE LRSCTEGKGE YTMEYSRYQP CLPSTQEDVI NKYLEATGQL PVKKGKAK N

Macromolecule #76: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.270934 KDa

Sequence

String:

MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS

Macromolecule #77: ICT1

• Macromolecule: Name: ICT1 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 38.427199 KDa

Sequence

String:

MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL GLPPKQKVKI FNVTENAVIK PGTPLYAAHF RPGQYVDVTA KTIGKGFQGV MRRWGFKGQP ATHGQTKTHR RP GAISTGD VARVWPGTKM PGQLGNIDRT AFGLKVWRIN TKHNIIYVNG SVPGHKNCLV KIKDSKLPAY KDFCKNLPFP TYF PDGDEE ALPEDLYDEN VCQPGAPSIT FT

Macromolecule #78: Mitochondrial ribosomal protein L4

• Macromolecule: Name: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.251469 KDa

Sequence

String:

MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP

Macromolecule #79: Mitochondrial ribosomal protein L9

• Macromolecule: Name: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 30.373449 KDa

Sequence

String:

MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I

Macromolecule #80: Mitochondrial ribosomal protein L10

• Macromolecule: Name: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 29.449689 KDa

Sequence

String:

MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL KGIPFLPLLG GCIDDTILSR QGFINYSKLP SLALVQGELV GGLTFLTAQT YSMLQHQPRQ LTALLDQYVK QQ QEGHPAV PASGQPEPPD PVPDP

Macromolecule #81: Mitochondrial ribosomal protein L11

• Macromolecule: Name: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.809271 KDa

Sequence

String:

MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK

Macromolecule #82: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.633801 KDa

Sequence

String:

MSSFSKAPQQ WATFARVWYL LDGKMQPPGK LAAMASVKLQ GLHKPVYHQL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQ VTAAQLHQKD PVAIVKLAIY GMLPKNLHRR TMMQRLHLFP DEDIPEDILK NLVEELPQPR KVPRRLDEYT Q EEIEAFPR VWSPPEDYRL

Macromolecule #83: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.955632 KDa

Sequence

String:

MAFCTGLRGP FAQVSRALSQ RCFSTTGSLS AIQKMTRVRV VDNSALGTTP YHRPPRCIHV YNKTGVGKVG DRILLAIRGQ KKKALIVGH RMPGPRMTPR FDSNNVVLLE DNGNPVGTRI KTPIPSSLRQ REGEFSKVLA IAQNFV

Macromolecule #84: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.481723 KDa

Sequence

String:

MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS

Macromolecule #85: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 28.680531 KDa

Sequence

String:

MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV

Macromolecule #86: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 19.268178 KDa

Sequence

String:

MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V

Macromolecule #87: Mitochondrial ribosomal protein L18

• Macromolecule: Name: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.582568 KDa

Sequence

String:

MALRSRFWAL LSICRNPGCR AAALSTSCKP ATNPETDPVE NEAVAPEFTN RNPRNLELLA VARKERGWGT VWPSREFWHR LRVIRTQHH IEALVEHRNG QVVVSASTRE WAIKKHLYST RNVVACESVG RVLAERCLEA GINFMVYHPT PWEAASDSIK R LQHAMTEG GVVLREPRRI YE

Macromolecule #31: 12S rRNA

• Macromolecule: Name: 12S rRNA / type: rna / ID: 31 / Number of copies: 2
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 5.350865 MDa

Sequence

String:

GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CACAGGUUUG GUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCUU A AAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC CCCACGGGAA AC AGCAGUG AUAAAAAUUA AGCCAUGAAC GAAAGUUUGA CUAAGUUAUA UUAAUUAGAG UUGGUAAAUC UCGUGCCAGC CAC CGCGGU CAUACGAUUA ACCCAAAUUA AUAGAUCCAC GGCGUAAAGA GUGUUUAAGA AAAAAAAUCA CAAUAGAGUU AAAU UAUAA CUAAGCUGUA AAAAGCCCUA GUUAAAAUAA AAUAACCCAC GAAAGUGACU CUAAUAAUCC UGACACACGA UAGCU AGGA CCCAAACUGG GAUUAGAUAC CCCACUAUGC CUAGCCCUAA ACCCAAAUAG UUACAUAACA AAACUAUUCG CCAGAG UAC UACUCGCAAC UGCCUAAAAC UCAAAGGACU UGGCGGUGCU UCACAUCCAC CUAGAGGAGC CUGUUCUAUA AUCGAUA AA CCCCGAUAGA CCUUACCAAC CCUUGCCAAU UCAGCCUAUA UACCGCCAUC UUCAGCAAAC CCUAAAAAGG AACAAUAG U AAGCACAAUC AUAGCACAUA AAAACGUUAG GUCAAGGUGU AGCUUAUGGG UUGGAAAGAA AUGGGCUACA UUUUCUACA UAAGAAUAUC CACCACACGA AAGUUUUUAU GAAACUAAAA ACCAAAGGAG GAUUUAGCAG UAAAUCAAGA AUAGAGUGCU UGAUUGAAU AAGGCCAUGA AGCACGCACA CACCGCCCGU CACCCUCCUC AAGCAUGUAG UAAUAAAAAU AACCUAUAUU C AAUUACAC AACCAUGCAA GAAGAGACAA GUCGUAACAA GGUAAGCAUA CUGGAAAGUG UGCUUGGAUU ACCAAAGCAU AG CUUAAAC UAAAGCACCU AGUUUACACC UAGAAGAUCC CACAAUGUAU GGGUACUUUG AACCAAAGCU AGCUCAACAU ACU AAACAA AUACAAAAAU ACACCAAAAU AAAAUAAAAC AUUCACCUAA CAUUAAAGUA UAGGAGAUAG AAAUUUUUAU CCUG ACGCU AUAGAGAUAG UACCGUAAGG GAAAGAUGAA AGAAUAAAAU AAAAGUAAAA AAAAGCAAAG AUUACCCCUU CUACC UUUU GCAUAAUGGU UUAACCAGAA AAAAUCUAAC AAAGAGAACU UUAGCUAGAU ACCCCGAAAC CAGACGAGCU ACCCAU GAG CAGUUUAAAA GAACCAACUC AUCUAUGUGG CAAAAUAGUG AGAAGACUUG UAGGUAGAGG UGAAAAGCCU AACGAGC CU GGUGAUAGCU GGUUGUCCGA GAAAGAAUUU UAGUUCAACC UUAAAAAUAC CCCAAAAACC CUAAAUUCCA AUGUAUUU U UAAGAGAUAG UCUAAAAAGG UACAGCUUUU UAGAAACGGA UACAACCUUG ACUAGAGAGU AAAAUCUUAA UACUACCAU AGUAGGCCUA AAAGCAGCCA UCAAUUGAGA AAGCGUUAAA GCUCAACAAA UUCACCAACA UAAUCCCAAA AACUAAUAAC AAACUCCUA GCCCAAUACC GGACUAAUCU AUUGAAACAU AGAAGCAAUA AUGUUAAUAU GAGUAACAAG AAGCCUUUCU C CUCGCACA CGCUUACAUC AGUAACUAAU AAUAUACUGA UAAUUAACAA CCAAUAAACC AAAACAACAC UAAAACGUUU AU UAAUUAC AUUGUUAACC CAACACAGGA GUGCACCAAG GAAAGAUUAA AAGAAGUAAA AGGAACUCGG CAAACACAAA CCC CGCCUG UUUACCAAAA ACAUCACCUC UAGCAUUACU AGUAUUAGAG GCAAUGCCUG CCCAGUGACA CCAGUUUAAC GGCC GCGGU AUUCUGACCG UGCAAAGGUA GCAUAAUCAC UUGUUCUCCA AAUAAGGACU UGUAUGAAUG GCCACACGAG GGUUU UACU GUCUCUUACU UCCAAUCAGU GAAAUUAACC UUCCCGUGAA GAGGCGGGAA UAAAAAAAUA AGACGAGAAG ACCCUA UGG AGCUUUAAUU AACUAUUCCA AAAGUUAAAC AACUCAACCA CAAAGGGAUA AAACAUAACU UAACAUGGAC UAGCAAU UU CGGUUGGGGU GACCUCGGAG UACAAAAAAC CCUCCGAGUG AUUUUAAUCU AGACAAACCA GUCAAAAUAA CCAUAACA U CACUUAUUGA UCCAAAAUUU UGAUCAACGG AACAAGUUAC CCUAGGGAUA ACAGCGCAAU CCUGUUCUAG AGUUCCUAU CGACAAUAGG GUUUACGACC UCGAUGUUGG AUCAGGACAC CCAAAUGGUG CAACCGCUAU UAAAGGUUCG UUUGUUCAAC GAUUAAAGU CCUACGUGAU CUGAGUUCAG ACCGGAGCAA UCCAGGUCGG UUUCUAUCUA UUAUAAAUUU CUCCCAGUAC G AAAGGACA AGAGAAAUGG GACCAACCUC ACAAACGCGU CUCAGAGAUA AUUAAUGAUU UAAUCUUAAC CUAAUUAACU CA UAAUAAA UCCAGCCCUA GAACAGGGCA CAUUAGGGUG GCAGAGACCG GUAAUUGCGU AAAACUUAAA CCUUUAUUAC CAG AGGUUC AACUCCUCUC CCUAAUAACA UGUUCAUAAU UAAUAUUCUA AGCCUAAUUA UUCCUAUCCU ACUGGCCGUA GCAU UCCUC ACCCUAGUAG AACGAAAAGU GCUAGGUUAU AUGCAACUAC GAAAAGGACC CAACGUUGUA GGCCCCUACG GCCUA CUCC AACCCAUCGC CGAUGCCCUA AAACUAUUCA CCAAAGAACC CCUACGACCA GCCACAUCCU CAAUCUCCAU GUUCAU UAU UGCACCAAUC CUAGCCUUAU CCCUAGCACU AACAAUAUGA GUUCCACUAC CAAUACCCUA CCCUCUAAUC AACAUAA AC CUAGGAGUAC UAUUCAUGCU AGCCAUGUCA AGCCUAGCAG UCUACUCUAU CCUAUGAUCA GGAUGAGCAU CCAACUCA A AAUACGCACU CAUCGGGGCC CUACGAGCAG UAGCCCAAAC AAUCUCAUAU GAAGUAACAC UAGCAAUCAU CCUACUAUC AGUACUCCUA AUAAAUGGAU CAUAUACUCU AUCAACCCUA AUCACAACAC AAGAGCACAU UUGAAUAAUC UUUACAUCCU GACCCCUAG CCAUAAUAUG AUUUAUCUCA ACCCUAGCAG AAACCAACCG AGCCCCGUUC GACCUUACAG AAGGAGAGUC A GAACUUGU AUCAGGCUUU AACGUAGAAU AUGCAGCCGG ACCUUUCGCC AUAUUCUUCA UAGCAGAAUA UGCCAACAUC AU CAUAAUA AAUGCAUUUA CAGCAAUUCU CUUCCUAGGA GCAUCCCACG ACCCACACAC ACCAGAACUA UAUACAAUCA ACU UCGUAC UAAAAACACU CGCAUUAACA AUCACCUUCC UAUGAAUCCG AGCAUCAUAC CCACGAUUCC GAUACGACCA ACUA AUACA UUUACUAUGA AAAAGCUUCC UGCCCCUAAC ACUAGCUCUA UGUAUAUGAC ACAUCUCACU CCCUAUUAUA ACAGC AAGC AUUCCCCCAC AAUCAUAGAA AUAUGUCUGA UAAAAGAGUU ACUUUGAUAG AGUAAAAAAU AGAGGUUCAA ACCCUC UUA UUUCUAGAAC AAUAGGACUC GAACCUAAAC CUGAGAAUUC AAAAUUCUCC GUGCUACCAA AAUACACCAC AUUCUAC AG UAAGGUCAGC UAAGCUAAGC UAUCGGGCCC AUACCCCGAA AAUGUUGGUU CAUACCCUUC CCAUACUAAU UAAUCCCA U UAUCUACACU ACCCUUAUCA UAACAGUAAU GUCCGGAACC AUACUAGUAA UAAUCAGCUC ACACUGACUA CUCAUCUGA AUCGGAUUCG AAAUAAACCU AUUAGCAAUA AUCCCAGUAU UAAUAAAAAA UUUUAACCCA CGAGCCACAG AAGCAGCCAC AAAAUAUUU CCUAACACAA GCCACAGCCU CCAUAAUACU AAUAAUAGCC AUCAUCAUCA ACCUCCUAUA UUCUGGCCAA U GGACCAUU ACAAAAAUAU UUAACCCAGU AGCAAUAACA AUAAUAACCA UGGCCCUAGC CAUAAAACUA GGACUCUCAC CU UUCCACU UCUGAGUCCC AGAAGUAACC CAAGGCAUUU CACUACAAGC AGGCCUACUG UUACUAACAU GACAAAAACU AGC CCCAUU AUCAGUACUA UGCCAAAUCU CACAAUCAAU CAACCCAAAC CUAAUACUAA CUAUAGCCAU AUUAUCAAUU UUAA UCGGA GGGUGAGGAG GGCUAAAUCA AACCCAACUU CGAAAAAUCA UAGCAUACUC AUCAAUCGCA CACAUAGGAU GAAUG ACAG CAGUAUUACC AUACAACACA ACCAUAACAA UCUUAAACCU ACUAAUUUAC AUCACAAUAA CACUAGCAAU AUUCAU ACU AUUAAUCCAC AGCUCAGCAA CCACAACUUU AUCCCUAUCC CAUACAUGAA ACAAAAUACC CGUCAUCACA AGCCUAA UA AUAGUAACCC UACUCUCAAU AGGAGGCCUG CCUCCACUAU CAGGAUUUAU GCCAAAAUGA AUAAUUAUUC AAGAAAUA A CAAAAAAUGA AAGCAUCAUC AUGCCAACAC UCAUAGCAAU AACAGCACUG CUAAACCUCU AUUUCUACAU ACGACUAGC CUACUCCUCC UCACUGACUA UGUUCCCAUC CACCAACAAC AUAAAAAUAA AAUGACAAUU CGAACACACA AAACAAAUAA AAUUACUUC CCACAAUAAU UGUAUUAUCA ACACUAGUCC UACCUAUAAC ACCAGCCCUC UCGUCCCUAA ACUAGGAAUU U AGGUUAAC ACAGACCAAG AGCCUUCAAA GCUCUAAGUA AGUACAAAGU ACUUAACUCC UGAAAACCUA AGGACUGCAG GA CUUAUCC UACAUCAAUU GAAUGCAAAU CAAACACUUU AAUUAAGCUA AAUCCUCACU AGAUUGGUGG GAUUACAUAC CCA CGAAAC UUUUAGUUAA CAGCUAAACA CCCUAAUCAA CUGGCUUCAA UCUACUUCUC CCGCCGCAGG AAAAAAAAGG CGGG AGAAG UCCCGGCAGA AUUGAAGCUG CUUCUUUGAA UUUGCAAUUC AACAUGAUAU UCACCACGGA ACUGGCAAAA AGAGG GCUU AACCUCUGUC UUUAGAUUUA CAGUCUAAUG CUUACUCAGC CAUUUUACCU AUGUUCGUAA AUCGUUGACU AUACUC AAC AAACCACAAA GACAUCGGCA CCCUGUACCU ACUAUUUGGU GCCUGAGCAG GAAUAGUGGG CACUGCCUUG AGCCUAC UA AUUCGCGCUG AACUAGGUCA GCCCGGAACC CUACUUGGCG AUGAUCAAAU CUAUAAUGUA AUUGUUACAG CUCAUGCC U UUGUAAUAAU CUUCUUUAUA GUAAUACCCA UUAUGAUUGG GGGUUUUGGU AACUGACUCG UACCGCUAAU AAUCGGAGC UCCCGAUAUG GCCUUUCCAC GUAUAAACAA CAUAAGUUUC UGACUACUUC CACCAUCCUU CCUAUUACUU CUGGCAUCCU CAAUAGUAG AAGCCGGAGC GGGUACUGGA UGAACUGUAU ACCCACCUUU AGCUGGAAAC UUAGCCCAUG CAGGGGCUUC A GUUGAUUU AACAAUUUUC UCCCUACACC UUGCAGGUGU AUCAUCAAUC CUAGGGGCUA UUAAUUUCAU UACCACAAUU AU UAACAUA AAACCCCCCG CAAUGUCUCA AUACCAAACA CCCCUGUUUG UCUGAUCAGU ACUAAUCACA GCCGUACUAC UUC UACUAU CCCUGCCAGU UCUAGCAGCU GGCAUUACUA UACUACUGAC AGACCGCAAC CUGAACACAA CCUUUUUUGA UCCA GCAGG UGGUGGAGAC CCUAUCCUUU AUCAACACUU GUUCUGAUUU UUCGGACACC CAGAAGUAUA CAUUCUCAUC UUACC AGGA UUCGGAAUAA UCUCCCACAU UGUAACCUAC UAUUCAGGUA AAAAAGAACC AUUUGGAUAU AUAGGCAUAG UAUGAG CCA UAAUGUCCAU UGGAUUCUUA GGUUUUAUUG UAUGGGCUCA CCACAUAUUC ACCGUAGGAA UAGACGUAGA UACCCGA GC AUACUUUACA UCUGCUACAA UAAUCAUUGC UAUUCCCACU GGAGUAAAAG UAUUUAGUUG AUUAGCUACC CUGCACGG C GGCAAUAUUA AAUGAUCACC CGCAAUACUA UGAGCUCUGG GCUUCAUCUU CCUAUUCACC GUAGGAGGUC UAACGGGCA UUGUACUAGC UAAUUCCUCC CUAGACAUUG UAUUACAUGA UACAUAUUAU GUAGUCGCAC ACUUCCACUA UGUCUUAUCU AUAGGAGCA GUGUUCGCCA UUAUAGGGGG CUUUGUUCAC UGAUUCCCCC UAUUCUCCGG GUACACACUC AACCAAGCAU G AGCAAAAA UUCACUUUGU AAUCAUAUUC GUAGGAGUAA AUAUAACAUU CUUUCCACAA CACUUUCUAG GACUAUCCGG AA UACCUCG ACGAUACUCC GAUUAUCCUG ACGCAUACAC AGCAUGAAAU ACUAUUUCCU CAAUAGGCUC AUUCAUCUCA CUA ACAGCA GUGAUAUUAA UAAUCUUCAU UAUCUGAGAA GCAUUCGCAU CAAAACGAGA AGUAUCUGCA GUAGAACUGA CAAG CACAA ACCUAGAAUG ACUACACGGA UGUCCUCCUC CCUAUCACAC AUUUGAAGAA CCAACAUAUA UCAACCUAAA AUAAG CAUA AGAAAGGAAG GAAUCGAACC CUCUCCCACU GGUUUCAAGC CAACGUCAUA ACCACUAUGU CUUUCUCGAU AAUCGA GGU AUUAGUAAAA UAUUACAUAA CUUUGUCGAA GUUAUAUUAU AGGUGAAAGC CCUAUAUGCC UCUAUGGCUU ACCCUUU CC AACUAGGCUU CCAAGACGCC ACUUCACCCA UCAUAGAAGA ACUCCUACAC UUUCACGAUC ACACCUUAAU AAUCGUAU U CUUAAUCAGC UCUUUAGUGU UAUAUAUCAU UUCACUCAUA CUAACAACAA AACUGACACA CACUAGCACA AUGGAUGCC CAAGAAGUAG AAACAAUUUG AACAAUCCUA CCCGCUAUUA UUCUAAUUCU UAUUGCCCUU CCAUCAUUAC GAAUCCUUUA UAUAAUAGA CGAAAUUAAU AACCCAGCCU UAACCGUAAA AACCAUAGGA CAUCAAUGAU ACUGAAGCUA CGAGUAUACA G ACUAUGAA GACCUCACCU UUGACUCAUA UAUAAUCCCC ACAUCAGAUC UUAAACCUGG AGAAAUACGA CUACUAGAAG UA GACAAUC GAGUUGUUCU ACCAAUAGAA AUAACAAUCC GAAUAUUAGU AUCCUCUGAA GACGUACUGC ACUCAUGAGC CGU CCCAUC CCUCGGUUUA AAAACAGAUG CUAUCCCAGG ACGACUAAAC CAAACAACUC UAAUAUCCAC ACGACCUGGC CUUU AUUAC GGACAGUGCU CAGAAAUCUG UGGAUCAAAC CACAGCUUCA UGCCCAUUGU ACUUGAACUU GUCCCAUUAA AGUAC UUCG AAAAAUGGUC AACAUCAAUA UUAACAGGUU CAUUGAGAAG CUAGUCAGCA CUAACCUUUU AAGUUAGAGA UCGGGA GCC UAAAUCUCCC CUCAAUGGUA UGCCACAACU AGAUACAUCC ACAUGAUUCA UUACAAUUAC AUCAAUAAUU AUAACAU UA UUUAUUUUAU UCCAACUAAA AAUCUCAAAC UACUCAUACC CAGCAAGCCC AGAAUCAAUU GAACUCAAAA CUCAAAAA C AUAGCACCCC UUGAGAAAUA AAAUGAACGA AAAUCUAUUU GCCUCUUUUA UUGCCCCCAC GAUAAUAGGA CUACCUAUU GUCACCUUAA UUAUUAUAUU CCCAAGCUUA CUAUUCCCAA CACCCAAACG ACUCAUUAAU AACCGCACAA UCUCGAUCCA ACAAUGAUU AAUCCAACUA ACAUCCAAAC AAAUAAUAGC UAUUCACAAC CAAAAAGGCC AAACCUGAUC ACUAAUACUU A UAUCUCUA AUUAUAUUCA UCGGCUCAAC AAACAUCCUA GGCCUACUAC CACACUCAUU CACACCCACC ACACAACUAU CA AUAAACC UGGGUAUAGC AAUCCCCCUA UGAUCAGCAA CCGUAUUCAC AGGAUUCCGC UAUAAAACCA AAACAUCACU AGC CCACUU UCUACCACAA GGAACACCCG CCCUAUUAAU UCCUAUGCUC GUAAUUAUUG AAACUAUUAG CCUAUUUAUU CAAC CAGUA GCCCUAGCCG UACGACUGAC AGCCAACAUU ACAGCAGGGC ACCUAUUAAU UCAUCUAAUU GGAGGGGCCA CAUUA GCAC UACUCAACAU CAACACUAUA ACAGCUUUUA UCACAUUUAC UAUCCUCAUU CUAUUAACUA UUCUUGAAUU UGCAGU AGC UCUGAUCCAA GCUUAUGUGU UUACACUGCU AGUAAGCUUA UACCUACACG ACAAUACAUA AUGACCCACC AAACACA UG CAUACCACAU AGUAAACCCA AGCCCAUGAC CACUUACCGG AGCCCUAUCA GCCCUUUUAA UAACAUCAGG CCUAACUA U AUGAUUCCAC UUUAACUCUA UACUCUUACU AUCUCUAGGA CUAUUAACCA AUACUUUGAC AAUAUACCAA UGGUGACGA GACAUUAUUC GAGAGAGCAC UUUCCAAGGC CACCACACAU CAGUUGUCCA AAAAGGCUUA CGAUACGGUA UAAUUUUAUU UAUUAUUUC CGAGGUUCUG UUCUUCACUG GAUUCUUUUG AGCUUUCUAC CACUCAAGCC UAGCACCAAC ACCCGAAUUA G GAGGUUGC UGACCACCAA CAGGAAUUCA CCCACUAAAC CCCCUAGAAG UACCCCUACU AAACACCUCA AUCCUCCUCG CC UCAGGAG UAUCCAUUAC CUGAGCCCAU CACAGCCUAA UAGAAGGGGA CCGAAAACAC AUAAUCCAAG CACUAUCCAU CAC CAUUGC ACUAGGCGUA UACUUCACCC UCCUCCAAGC CUCAGAAUAU UACGAAGCAC CAUUCACAAU CUCCGACGGA GUGU AUGGA UCCACUUUCU UUGUGGCUAC AGGGUUUCAC GGGUUGCACG UAAUCAUCGG AUCUACUUUC CUAGCAGUAU GCUUA CUAC GACAACUAAA AUUCCACUUC ACAUCCAACC ACCACUUCGG CUUUGAAGCC GCAGCCUGAU ACUGACACUU CGUAGA UGU AGUUUGACUA UUCCUUUACG UAUCAAUCUA UUGAUGAGGA UCCUACUCUU UUAGUAUUAA GUAGUACAAU UGACUUC CA AUCAAUCAGU UUCGGUAAAC UCCGAAAAAG AGUAAUAAAU AUUAUACUAA CACUACUCAC AAACGUAACC CUAGCCUC C CUACUCGUAC UAAUCGCAUU CUGACUACCC CAACUAAACG CAUAUUCAGA AAAAACAAGC CCAUAUGAAU GUGGAUUUG ACCCCAUAGG AUCAGCACGC CUCCCAUUCU CAAUAAAAUU UUUCCUAGUA GCCAUUACAU UUCUCCUUUU UGAUCUAGAA AUCGCCCUU CUCCUUCCCC UACCAUGAGC AUCCCAAACA AACAAUCUAA AAACAAUACU UACAAUAGCA CUAUUCCUUC U UAUCCUAC UAGCAGCAAG CCUAGCAUAC GAAUGAACCC AAAAAGGCCU AGAAUGAGCA GAAUAUGAUA AUUAGUUUAA AA CAAAACA AAUGAUUUCG ACUCAUUAGA CUAUGAUUUA CUUCAUAAUU AUCAAGUGCC AUUAGUAUAU AUAAACAUCA UUA UAGCAU UCGCGAUCGC CCUUGCAGGG UUACUUAUAU AUCGAUCUCA CUUAAUAUCU UCACUACUAU GCCUAGAAGG AAUG AUACU AUCACUAUUC AUCAUAUCGA CUCUAAUUAU CCUAAACACA CACUUCACCC UAGCUAACAU AAUACCCAUU AUUUU ACUA GUGUUUGCAG CCUGCGAAGC UGCACUAGGC CUGUCACUAC UAGUAAUAGU AUCCAACACA UACGGUACCG AUUACG UCC AAAACUUAAA CCUUUUACAA UGCUAAAAAU UAUUAUCCCA ACAACAAUAC UACUACCCAU AACAUGAAUA UCUAAAC AC AACAUAAUCU GAAUCAAUGC AACAGUACAU AGUCUCCUCA UUAGCCUGAU CAGUCUAUCC CUACUAAACC AACUAGGC G AAAACAGCCU UAAUUUUUCC UUAACAUUCU UCUCCGACUC ACUAUCAGCA CCCCUACUAG UUCUAACCAC AUGACUCCU CCCCCUUAUA CUAAUAGCUA GCCAAUCUCA CCUAUCAAAA GAAACCACAA CCCGAAAAAA ACUAUAUAUU ACCAUACUAA UCCUACUAC AACUAUUCCU AAUUAUAACC UUCACCGCCA CCGAACUAAU CCUAUUCUAU AUCCUAUUCG AAGCAACACU A GUACCCAC ACUAAUUAUC AUCACACGCU GAGGAAACCA AACAGAACGA CUCAAUGCAG GACUUUAUUU CCUAUUCUAC AC CCUAGCA GGAUCCCUAC CACUGCUAGU AGCACUAGUU UAUAUCCAAA AUACCACAGG CUCACUAAAC UUCUUAAUUA UCC AUUACU GAUCCCACCC AUUAUCCAAC UCUUGAUCAA ACAUUUUUAU AUGAUUAGCA UGCAUCAUAG CCUUCAUAGU AAAA AUACC UCUGUACGGA CUCCAUCUUU GACUGCCAAA AGCCCAUGUA GAAGCCCCCA UUGCAGGUUC AAUAGUACUU GCAGC CGUA CUGCUAAAAC UCGGAGGCUA UGGCAUAAUG CGAAUCACUA CUAUUCUAAA CCCACUAACA AACUACAUAG CCUAUC CAU UCCUCAUGCU UUCCAUAUGA GGCAUAAUCA UAACCAGCUC UAUCUGCUUA CGUCAAACCG ACCUAAAAUC CUUAAUC GC CUAUUCAUCA GUAAGUCAUA UAGCACUUGU AAUCGUAGCA AUCAUAAUUC AAACCCCCUG AAGCUUCAUA GGGGCCAC A GCUCUCAUAA UUGCCCACGG ACUAACAUCC UCCAUACUAU UCUGCCUAGC CAACACUAAC UAUGAACGAG UACACAGCC GAACCAUAAU CCUGGCCCGA GGACUGCAAA CACUCCUACC ACUCAUAGCA ACAUGAUGAC UAGUAGCAAG CCUCACAAAC CUAGCCCUA CCCCCAUCCA UCAAUCUAAU CGGAGAAUUA UUUAUCAUCA CAGCAUCAUU UUCAUGAUCC AACAUCACAA U UAUUCUCA UAGGAAUAAA CAUAAUAAUU ACAGCCCUCU ACUCUCUCUA CAUACUAAUU ACUACACAAC GAGGAAAAUA CA CCCACCA CAUUAACAAC AUCAAACCCU CAUUCACACG AGAAAACGCC CUCAUAGCCC UACAUAUUCU ACCACUACUA CUA CUGACC UUAAACCCUA AAAUAAUCCU AGGACCCCUU UACUGUAGAU AUAGUUUAAU AAAAACCCUA GAUUGUGAAU CUAG UAAUA GAAAAUUAAA UAUUCUUAUC UACCGAAAAA GUUUGCAAGA ACUGCUAACU CAUGCUUCCA CACUUAAAAA UGUGG CUUU UUCAACUUUU AAAGGAUAAC AGCUAUCCGU UGGUCUUAGG AACCAAAAAA UUGGUGCAAC UCCAAAUAAA AGUAAU AAA CCCAUUCGCC UCACUCACAU UAACCACACU AACUAUUCUA ACCAUCCCAA UUAUAAUAUC CAACUCAAAC AUCUACA AA ACUAACCUUU ACCCUAACUA CGUAAAAACC ACCGUAUCCU ACGCCUUCAC UCUCAGCCUA GUCCCCUUAC UAAUAUUU A UACACACAGG CCAAGAAAUA AUCAUUUCAA ACUGACAUUG AAUAACCCUA CAGACCGUAG AACUCUCUCU UAGCUUCAA AAUAGACUAU UUCUCAGUAA UAUUCAUUCC CGUAGCACUA UUCGUCACAU GAUCAAUUAU AGAAUUCUCC AUAUGAUACA UACACUCAG ACCCCUUCAU CAACCGAUUC UUUAAAUACC UACUACUAUU CUUAAUCACU AUAAUAAUCC UCGUAACCGC C AACAACCU CUUCCAACUA UUUAUCGGAU GAGAAGGCGU AGGAAUCAUA UCAUUCCUGC UAAUUGGAUG AUGACACGGA CG AACAGAC GCCAACACAG CUGCACUACA AGCAAUCCUA UACAACCGCA UCGGAGACAU UGGAUUUGUC CUAUCCAUAG CAU GAUUCC UAACCCACUC AAACGCAUGA GAUCUUCAAC AAAUCUUUAU ACUAAACAAU GAAUGCCCAA ACAUACCAUU AAUC GGCCU ACUCCUAGCU GCAGCAGGAA AAUCAGCUCA AUUCGGACUA CAUCCCUGAU UGCCCUCAGC AAUAGAAGGC CCAAC UCCC GUAUCAGCAU UACUACACUC CAGUACAAUA GUAGUAGCAG GGGUAUUUCU ACUCAUCCGC UUCUACCCCU UAAUAG AAA CUAACAAACU AGUUCAAACU AUAACACUAU GCCUAGGAGC UAUCACCACC UUAUUUACAG CACUAUGUGC AAUCACA CA AAAUGAUAUC AAAAAAAUCG UAGCCUUCUC AACUUCAAGC CAACUAGGCU UGAUAAUAGU GACAAUCGGC AUCAACCA A CCCCACCUAG CAUUUCUCCA CAUCUGCAUG CACGCUUUCU UCAAAGCAAU ACUAUUCAUA UGCUCCGGAU CCAUUAUCC ACAGCCUCAA UGACGAACAA GACAUCCGAA AAAUAGGCGG ACUGUAUAAA GCAAUACCAU UCACAACAAC AGCACUAAUU AUUGGAAGC CUGGCAUUAA CAGGAAUGCC UUAUCUCACA GGAUUCUACU CAAAAGACCU UAUCAUUGAA GCAGUAAAUA U AUCCUACA CAAACGCCUG AGCCCUACUA AUAACAUUAA UUGCCACAUC CCUAACCGCU GCCUACAGCA CUCGAAUUAU CU UCUUUGC AUUCCUAGGG AAACCACGUU UCCCACCCCU AGUCCUAAUU AAUGAAAAUA ACCCCCUACU AAUUAACUCU AUU AAACGC CUUUUAAUCG GAAGCAUUUU CGCUGGCUUU AUCAUCUCCA ACAACAUCCC ACCAAUAACA GUACCAAACA CAAC AAUAC CCCUUUACAU AAAAAUAACA GCCCUAAUCG UAACCAUCAU AGGAUUCAUA CUAGCCCUAG AGCUAAACAA CACAA CCUA CUACCUGAAA UUUAAAUACC CCUCACAAAC AUACAAAUUU UCCAACAUAC UAGGAUAUUA UCCCUCCAUC AUACAC CGC CUACCAACAU ACCACAACCU AUCUAUAAGC CAAAAAUCCG CAUCAUCAUU ACUAGACUUA AUUUGACUAG AAACUAU UC UACCAAAAAC AACCUCUUUC AUCCAAAUAA AAAUAUCAAU CAUAGUAUCA AAUCAAAAAG GCCUAAUCAA ACUAUACU U UCUCUCCUUC CUAAUCACUA UUAUAAUCAG CAUAACACUA UUUAAUUACC ACGAGUAAUC UCUAUAAUAA CAACAACUC CAAUAAGCAA UGAUCAACCA GUAACAAUAA CUAAUCAAGU ACCAUAACUA UAUAAAGCAG CAAUCCCCAU AGCUUCCUCA CUAAAAAAC CCCGAAUCAC CCGUAUCAUA AAUUACUCAA UCCCCAAGCC CAUUAAACUU AAAGAUAAUU UCUACUUCCU C UUCCUUCA ACGCAUAAUA AACCAUACAA AACUCCAUUA UUAAACCAGA AACAAAUGCU CCAAAAACAG UCUUAUUAGA AA CUCAAAC CUCAGGAUAC AUCUCAGUAG CCAUAGCAGU AGUAUAACCA AAAACCACCA ACAUACCCCC CAAAUAAAUC AAA AACACC AUUAAACCUA AAAAAGACCC ACCAAAAUUC AAUACAAUAC CACAACCAAC UCCACCACUU ACAAUCAACC CAAG UCCAC CAUAAAUAGG AGAGGGCUUA GAAGAAAAAC CAACAAACCC AAUAACAAAA AUAGUACUUA AAAUAAAUGC AAUAU ACAU UGUCAUUAUU CUCACAUGGA AUUUAACCAC GACCAAUGAC AUGAAAAAUC AUCGUUGUAC UUCAACUACA AGAACC UUA AUGACCAACA UCCGAAAAUC ACACCCACUA AUAAAAAUUA UCAACAACGC AUUCAUUGAC CUCCCAGCCC CCUCAAA CA UCUCAUCAUG AUGAAACUUC GGUUCCCUCU UAGGCAUCUG CCUAAUCUUG CAAAUCCUAA CAGGCCUGUU CUUAGCAA U ACAUUACACA UCAGACACAA CAACAGCUUU CUCAUCAGUU ACACACAUUU GUCGAGACGU AAAUUACGGA UGAGUUAUU CGCUAUCUAC AUGCAAACGG AGCAUCCAUA UUCUUUAUUU GCCUAUUCAU CCACGUAGGC CGAGGUCUAU ACUACGGAUC CUAUAUAUU CCUAGAAACA UGAAACAUUG GAGUAGUCCU ACUAUUUACC GUUAUAGCAA CAGCCUUCAU AGGCUACGUC C UGCCCUGA GGACAAAUAU CAUUCUGAGG AGCUACGGUC AUCACAAAUC UACUAUCAGC UAUCCCUUAU AUCGGAACAG AC CUCGUAG AAUGAAUCUG AGGGGGCUUU UCCGUCGACA AAGCAACCCU CACACGAUUC UUCGCCUUCC ACUUUAUCCU GCC AUUCAU CAUUACCGCC CUCGCAGCCG UACAUCUCAU AUUCCUGCAC GAAACCGGAU CCAACAACCC UACCGGAAUC UCAU CAGAC AUAGACAAAA UUCCAUUUCA CCCAUACUAC ACUAUUAAAG ACAUUCUAGG AGCCUUAUUU AUAAUACUAA UCCUA CUAA UCCUUGUACU AUUCUCACCA GACCUACUAG GAGACCCAGA CAACUACACC CCAGCAAACC CACUAAACAC CCCACC CCA UAUUAAACCA GAAUGAUAUU UCUUAUUCGC CUACGCUAUU CUACGUUCAA UUCCUAAUAA ACUAGGUGGA GUGUUGG CC CUAGUAGCCU CCAUCCUAAU CCUAAUUUUA AUGCCCAUAC UGCACACAUC CAAACAACGA GGCAUAAUAU UUCGACCA C UAAGUCAAUG CCUAUUCUGA AUACUAGUAG CAGACCUCAU UACACUAACA UGAAUUGGAG GACAACCCGU AGAACACCC GUUCAUCAUC AUCGGCCAAC UAGCCUCCAU CUUAUACUUC CUAAUCAUUC UAGUAUUGAU ACCAAUCACU AGCAUCAUCG AAAACAACC UAUUAAAAUG AAGAGUCUUC GUAGUAUAUA AAAUACCCUG GUCUUGUAAA CCAGAAAAGG AGGGCCACCC C UCCCCAAG ACUCAAGGAA GGAGACUAAC UCCGCCAUCA GCACCCAAAG CUGAAAUUCU AACUAAACUA UUCCCUGCAA CC AAAACAA GCAUUCCAUU CGUAUGCAAA CCAAAACGCC AAGUACUUAA UUACUAUCUU UAAAACAAAA AAACCCAUAA AAA UUGCGC ACAAACAUAC AAAUAUGUGA CCCCAAAAAU UUUACCAUUG AAAACCAAAA AAUCUAAUAU ACUAUAACCC UAUG UACGU CGUGCAUUAA CUGCUAGUCC CCAUGCAUAU AAGCAUGUAC AUAUUAUUAU UAAUAUUACA UAGUACAUAU UAUUA UUGA UCGUACAUAG CACAUAUCAU GUCAAAUAAC UCCAGUCAAC AUGCAUAUCA CCACCACUAG AUCACGAGCU UAAUUA CCA UGCCGCGUGA AACCAGCAAC CCGCUUGGCA GGGAUCCCUC UUCUCGCUCC GGGCCCAUAA ACCGUGGGGG UUUCUAU UG AUGAACUUUA ACAGGCAUCU GGUUCUUACU UCAGGACCAU CUCACCUAAA AUCGGCCACU CUUUCCCCUU AAAUAAGA C AUCUCGAUGG ACUAAUGACU AAUCAGCCCA UGCUCACACA UAACUGAGGU UUCAUACAUU UGGUAUUUUU UAAUUUUUG GGGAUGCUUA GACUCAGCCA UGGCCGUCAA AGGCCCUAAC ACAGUCAAAU CAAUUGUAGC UGGACUUCAU GGAACUCAUG AUCCGGCAC GACAAUCCAA ACAAGGUGCU AUUCAGUCAA UGGUUACGGG ACAUAACGUA CAUACACGUG CGUACACGUG C GUACACGU GCGUACACGU GCGUACACGU GCGUACACGU GCGUACACGU GCGUACACGU GCGUACACGU GCGUACACGU GC GUACACG UGCGUACACG UGCGUACACG UGCGUACACG UGCGUACACG UGCGUACACG UGCGUACACG UGCGUACACG UGC GUACAC GUGCGUACAC GUGCGUACAC GUGCGUACAC GUGCGUACAC GUGCGUACAC GUGCGUACAC GUGCGUACAC GUGC GUACA CGUGCGUACA CGUGCGUACA CGUGCGUACA CGCGCAUAUA AGCAGGUAAA UUAUUAGCUC AUUCAAACCC CCCUU ACCC CCCAUUAAAC UUAUGCUCUA CACACCCUAU AACGCCUUGC CAAACCCCAA AAACAAAGCA GAGUGUACAA AUACAA UAA GCCUAACUUA CACUAAACAA CAUUUAACAA CACAAACCAC CAUAUCUUAU AAAACACUUA CUUAAAUACG UGCUACG AA AGCAGGCACC UACCCCCCUA GAUUUUUACG CCAAUCUACC AUAAAUAAAU UUAAAAUUAC AACACAAUAA CCUCCCAA A AUAUAAGCAC CUAUUUAAGU AUACGCCCAC AAUCUGAAUA UAGCUUAUA

Macromolecule #46: tRNAMet

• Macromolecule: Name: tRNAMet / type: rna / ID: 46 / Number of copies: 2
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.664498 KDa

Sequence

String:

AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A

Macromolecule #47: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 47 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.892173 KDa

Sequence

String:

CUGAUG

Macromolecule #74: CP tRNA-Phe

• Macromolecule: Name: CP tRNA-Phe / type: rna / ID: 74 / Number of copies: 1
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 23.402018 KDa

Sequence

String:

GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA

Macromolecule #88: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 88 / Number of copies: 325 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #89: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 89 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #90: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 90 / Number of copies: 3 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

Macromolecule #91: N-FORMYLMETHIONINE

• Macromolecule: Name: N-FORMYLMETHIONINE / type: ligand / ID: 91 / Number of copies: 1 / Formula: FME
• Molecular weight: Theoretical: 177.221 Da

Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

Macromolecule #92: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 92 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #93: GUANOSINE-5'-MONOPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 93 / Number of copies: 1 / Formula: 5GP
• Molecular weight: Theoretical: 363.221 Da

Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE / Guanosine monophosphate

Macromolecule #94: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

• Macromolecule: Name: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 94 / Number of copies: 1 / Formula: GNP
• Molecular weight: Theoretical: 522.196 Da

Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

Macromolecule #95: water

• Macromolecule: Name: water / type: ligand / ID: 95 / Number of copies: 5 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.017 mg/mL
• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: OTHER
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 40.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12858

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-6ydw:
55S mammalian mitochondrial ribosome with mtEFG1 and two tRNAMet (TI-POST)