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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-10612 | ||||||||||||
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Title | Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 2 | ||||||||||||
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Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.8 Å | ||||||||||||
![]() | Krupp F / Banchenko S / Buerger J / Mielke T / Spahn CMT / Schwefel D | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 2 Authors: Krupp F / Banchenko S / Buerger J / Mielke T / Spahn CMT / Schwefel D | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 9.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.1 KB 8.1 KB | Display Display | ![]() |
Images | ![]() | 74.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Cryo-EM map of DDB1:DCAF:Vpr State 1
Entire | Name: Cryo-EM map of DDB1:DCAF:Vpr State 1 |
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Components |
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-Supramolecule #1: Cryo-EM map of DDB1:DCAF:Vpr State 1
Supramolecule | Name: Cryo-EM map of DDB1:DCAF:Vpr State 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI POLARA 300 |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.07) / Number images used: 29118 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |