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- EMDB-10613: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3 -

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Basic information

Entry
Database: EMDB / ID: EMD-10613
TitleCryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Map dataCryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Sample
  • Complex: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Biological speciesSpodoptera frugiperda (fall armyworm)
Methodsingle particle reconstruction / cryo EM / Resolution: 8.8 Å
AuthorsKrupp F / Banchenko S / Buerger J / Mielke T / Spahn CMT / Schwefel D
Funding support Germany, 3 items
OrganizationGrant numberCountry
German Research Foundation (DFG)25065445 Germany
European Molecular Biology Organization (EMBO)190-2016 Germany
German Research Foundation (DFG)Emmy Noether Programme 280022178 Germany
CitationJournal: To Be Published
Title: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Authors: Krupp F / Banchenko S / Buerger J / Mielke T / Spahn CMT / Schwefel D
History
DepositionJan 15, 2020-
Header (metadata) releaseJan 27, 2021-
Map releaseJan 27, 2021-
UpdateApr 14, 2021-
Current statusApr 14, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.013
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.013
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10613.png

Downloads & links

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Map

FileDownload / File: emd_10613.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Voxel sizeX=Y=Z: 1.25 Å
Density
Contour LevelBy AUTHOR: 0.013 / Movie #1: 0.013
Minimum - Maximum-0.0129787875 - 0.044642024
Average (Standard dev.)0.00010763236 (±0.0014924109)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 400.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.251.251.25
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z400.000400.000400.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-0.0130.0450.000

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Supplemental data

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Sample components

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Entire : Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3

EntireName: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3
Components
  • Complex: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3

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Supramolecule #1: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3

SupramoleculeName: Cryo-EM map of DDB1:DCAF1:Vpr:Cullin4:Roc1 State 3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Spodoptera frugiperda (fall armyworm)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 8.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.07) / Number images used: 19125

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