+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-0948 | |||||||||
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タイトル | Cryo-EM structure of a pre-60S ribosomal subunit - state C | |||||||||
マップデータ | Cryo-EM structure of a human pre-60S ribosomal subunit - state C | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 positive regulation of protein localization to nucleolus / preribosome binding / lamin filament / positive regulation of RNA biosynthetic process / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / miRNA-mediated post-transcriptional gene silencing / miRNA-mediated gene silencing by inhibition of translation / eukaryotic 80S initiation complex / negative regulation of protein neddylation ...positive regulation of protein localization to nucleolus / preribosome binding / lamin filament / positive regulation of RNA biosynthetic process / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / miRNA-mediated post-transcriptional gene silencing / miRNA-mediated gene silencing by inhibition of translation / eukaryotic 80S initiation complex / negative regulation of protein neddylation / translation at presynapse / axial mesoderm development / ribosomal protein import into nucleus / negative regulation of formation of translation preinitiation complex / 90S preribosome assembly / positive regulation of kinase activity / TORC2 complex binding / GAIT complex / middle ear morphogenesis / regulation of glycolytic process / maturation of 5.8S rRNA / A band / regulation of reactive oxygen species metabolic process / alpha-beta T cell differentiation / cytoplasmic side of rough endoplasmic reticulum membrane / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / negative regulation of ubiquitin protein ligase activity / optic nerve development / intrinsic apoptotic signaling pathway in response to oxidative stress / response to aldosterone / retinal ganglion cell axon guidance / homeostatic process / G1 to G0 transition / macrophage chemotaxis / lung morphogenesis / male meiosis I / Protein hydroxylation / ribosomal large subunit binding / Peptide chain elongation / preribosome, large subunit precursor / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / blastocyst development / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / protein localization to nucleus / ribosomal large subunit export from nucleus / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / protein-RNA complex assembly / cellular response to interleukin-4 / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal subunit export from nucleus / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / Maturation of protein E / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / Maturation of protein E / ER Quality Control Compartment (ERQC) / maturation of LSU-rRNA / Myoclonic epilepsy of Lafora / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Glycogen synthesis / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / TICAM1,TRAF6-dependent induction of TAK1 complex / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / TICAM1-dependent activation of IRF3/IRF7 / NOTCH2 Activation and Transmission of Signal to the Nucleus / cytosolic ribosome / Regulation of FZD by ubiquitination / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / VLDLR internalisation and degradation / embryo implantation 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) / Human (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.09 Å | |||||||||
データ登録者 | Liang X / Zuo M / Zhang Y / Li N / Ma C / Dong M / Gao N | |||||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2020 タイトル: Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. 著者: Xiaomeng Liang / Mei-Qing Zuo / Yunyang Zhang / Ningning Li / Chengying Ma / Meng-Qiu Dong / Ning Gao / 要旨: Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which ...Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_0948.map.gz | 39.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-0948-v30.xml emd-0948.xml | 65.9 KB 65.9 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_0948.png | 84.9 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-0948 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0948 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_0948_validation.pdf.gz | 372.6 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_0948_full_validation.pdf.gz | 372.2 KB | 表示 | |
XML形式データ | emd_0948_validation.xml.gz | 7.8 KB | 表示 | |
CIF形式データ | emd_0948_validation.cif.gz | 9 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0948 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0948 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_0948.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM structure of a human pre-60S ribosomal subunit - state C | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.058 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : Cryo-EM structure of a human pre-60S ribosomal subunit state - C
+超分子 #1: Cryo-EM structure of a human pre-60S ribosomal subunit state - C
+超分子 #2: Cryo-EM structure of a human pre-60S ribosomal subunit state - C
+超分子 #3: 60S ribosomal export protein NMD3
+分子 #1: Zinc finger protein 622
+分子 #3: 60S ribosomal export protein NMD3
+分子 #5: Eukaryotic translation initiation factor 6
+分子 #7: 60S ribosomal protein L10a
+分子 #8: 60S ribosomal protein L3
+分子 #9: 60S ribosomal protein L29
+分子 #10: 60S ribosomal protein L4
+分子 #11: 60S ribosomal protein L30
+分子 #12: 60S ribosomal protein L34
+分子 #13: 60S ribosomal protein L7a
+分子 #14: 60S ribosomal protein L35
+分子 #15: 60S ribosomal protein L9
+分子 #16: 60S ribosomal protein L10-like
+分子 #17: 60S ribosomal protein L36
+分子 #18: 60S ribosomal protein L27a
+分子 #19: 60S ribosomal protein L37
+分子 #20: 60S ribosomal protein L11
+分子 #21: 60S ribosomal protein L38
+分子 #22: 60S ribosomal protein L39
+分子 #23: 60S ribosomal protein L13
+分子 #24: Ubiquitin-60S ribosomal protein L40
+分子 #25: 60S ribosomal protein L14
+分子 #26: 60S ribosomal protein L31
+分子 #27: 60S ribosomal protein L15
+分子 #28: 60S ribosomal protein L13a
+分子 #29: 60S ribosomal protein L36a
+分子 #30: 60S ribosomal protein L37a
+分子 #31: 60S ribosomal protein L17
+分子 #32: 60S ribosomal protein L18
+分子 #33: 60S ribosomal protein L19
+分子 #34: 60S ribosomal protein L18a
+分子 #35: 60S ribosomal protein L21
+分子 #36: 60S ribosomal protein L22
+分子 #37: 60S ribosomal protein L23
+分子 #38: 60S ribosomal protein L24
+分子 #39: 60S ribosomal protein L23a
+分子 #40: 60S ribosomal protein L26
+分子 #41: 60S ribosomal protein L27
+分子 #42: 60S ribosomal protein L28
+分子 #43: 60S ribosomal protein L8
+分子 #44: 60S ribosomal protein L5
+分子 #45: 60S ribosomal protein L32
+分子 #46: 60S ribosomal protein L35a
+分子 #47: 60S ribosomal protein L6
+分子 #48: 60S ribosomal protein L7
+分子 #2: 28S rRNA
+分子 #4: 5S rRNA
+分子 #6: 5.8S rRNA
+分子 #49: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 64.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.09 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 21707 |
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初期 角度割当 | タイプ: NOT APPLICABLE |
最終 角度割当 | タイプ: PROJECTION MATCHING |