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TitleStructural snapshots of human pre-60S ribosomal particles before and after nuclear export.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3542, Year 2020
Publish dateJul 15, 2020
AuthorsXiaomeng Liang / Mei-Qing Zuo / Yunyang Zhang / Ningning Li / Chengying Ma / Meng-Qiu Dong / Ning Gao /
PubMed AbstractRibosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which ...Ribosome biogenesis is an elaborate and energetically expensive program that involve two hundred protein factors in eukaryotes. Nuclear export of pre-ribosomal particles is one central step which also serves as an internal structural checkpoint to ensure the proper completion of nuclear assembly events. Here we present four structures of human pre-60S particles isolated through a nuclear export factor NMD3, representing assembly stages immediately before and after nuclear export. These structures reveal locations of a dozen of human factors, including an uncharacterized factor TMA16 localized between the 5S RNA and the P0 stalk. Comparison of these structures shows a progressive maturation for the functional regions, such as peptidyl transferase centre and peptide exit tunnel, and illustrate a sequence of factor-assisted rRNA maturation events. These data facilitate our understanding of the global conservation of ribosome assembly in eukaryotes and species-specific features of human assembly factors.
External linksNat Commun / PubMed:32669547 / PubMed Central
MethodsEM (single particle)
Resolution3.09 - 3.23 Å
Structure data

0948

6lqm

EMDB-0948, PDB-6lqm:
Cryo-EM structure of a pre-60S ribosomal subunit - state C
Method: EM (single particle) / Resolution: 3.09 Å

0963

6lsr

EMDB-0963, PDB-6lsr:
Cryo-EM structure of a pre-60S ribosomal subunit - state B
Method: EM (single particle) / Resolution: 3.13 Å

0964

6lss

EMDB-0964, PDB-6lss:
Cryo-EM structure of a pre-60S ribosomal subunit - state preA
Method: EM (single particle) / Resolution: 3.23 Å

0978

6lu8

EMDB-0978, PDB-6lu8:
Cryo-EM structure of a human pre-60S ribosomal subunit - state A
Method: EM (single particle) / Resolution: 3.13 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / 60S / pre-60S / pre-ribosome / human 60S / human pre-ribosome / NMD3 / human NMD3

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