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- EMDB-0649: Electron cryo-microscopy of the eukaryotic translation initiation... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0649 | |||||||||
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Title | Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to translation initiation factor 2 from Homo sapiens | |||||||||
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![]() | eIF2B bound to eIF2
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Function / homology | ![]() translation initiation ternary complex / glial limiting end-foot / response to manganese-induced endoplasmic reticulum stress / negative regulation of translational initiation in response to stress / positive regulation of type B pancreatic cell apoptotic process / guanyl-nucleotide exchange factor complex / eukaryotic translation initiation factor 2 complex / eukaryotic translation initiation factor 2B complex / translation factor activity, RNA binding / multi-eIF complex ...translation initiation ternary complex / glial limiting end-foot / response to manganese-induced endoplasmic reticulum stress / negative regulation of translational initiation in response to stress / positive regulation of type B pancreatic cell apoptotic process / guanyl-nucleotide exchange factor complex / eukaryotic translation initiation factor 2 complex / eukaryotic translation initiation factor 2B complex / translation factor activity, RNA binding / multi-eIF complex / astrocyte differentiation / cytoplasmic translational initiation / astrocyte development / PERK-mediated unfolded protein response / negative regulation of guanyl-nucleotide exchange factor activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Nguyen H / Kenner L / Frost A | |||||||||
Funding support | ![]()
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![]() | ![]() Title: eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response. Authors: Lillian R Kenner / Aditya A Anand / Henry C Nguyen / Alexander G Myasnikov / Carolin J Klose / Lea A McGeever / Jordan C Tsai / Lakshmi E Miller-Vedam / Peter Walter / Adam Frost / ![]() ![]() Abstract: The integrated stress response (ISR) tunes the rate of protein synthesis. Control is exerted by phosphorylation of the general translation initiation factor eIF2. eIF2 is a guanosine triphosphatase ...The integrated stress response (ISR) tunes the rate of protein synthesis. Control is exerted by phosphorylation of the general translation initiation factor eIF2. eIF2 is a guanosine triphosphatase that becomes activated by eIF2B, a two-fold symmetric and heterodecameric complex that functions as eIF2's dedicated nucleotide exchange factor. Phosphorylation converts eIF2 from a substrate into an inhibitor of eIF2B. We report cryo-electron microscopy structures of eIF2 bound to eIF2B in the dephosphorylated state. The structures reveal that the eIF2B decamer is a static platform upon which one or two flexible eIF2 trimers bind and align with eIF2B's bipartite catalytic centers to catalyze nucleotide exchange. Phosphorylation refolds eIF2α, allowing it to contact eIF2B at a different interface and, we surmise, thereby sequestering it into a nonproductive complex. | |||||||||
Validation Report | ![]() ![]() ![]() ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
Header (meta data in XML format) |
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Images |
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Archive directory |
-Related structure data
Related structure data | ![]() 6o81CM ![]() 0651C ![]() 0664C ![]() 6o85C ![]() 6o9zC C: citing same article ( M: atomic model generated by this map |
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Similar-shape strucutres |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.822 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
+Entire eIF2B bound to eIF2
+Component #1: protein, eIF2B bound to eIF2
+Component #2: protein, Translation initiation factor eIF-2B subunit epsilon
+Component #3: protein, Translation initiation factor eIF-2B subunit beta
+Component #4: protein, Translation initiation factor eIF-2B subunit delta
+Component #5: protein, Translation initiation factor eIF-2B subunit alpha
+Component #6: protein, Translation initiation factor eIF-2B subunit gamma
+Component #7: protein, Eukaryotic translation initiation factor 2 subunit 1
+Component #8: protein, Eukaryotic translation initiation factor 2 subunit 3
+Component #9: protein, Translation initiation factor eiF2 beta-subunit
+Component #10: ligand, 2-(4-chloranylphenoxy)-~{N}-[4-[2-(4-chloranylphenoxy)eth...
-Experimental details
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Sample preparation
Specimen | Specimen state: Particle / Method: ![]() |
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Sample solution | pH: 7.5 |
Support film | unspecified |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: OTHER |
Camera | Detector: GATAN K2 SUMMIT (4k x 4k) |
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Image processing
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3D reconstruction | Resolution: 3.21 Å / Resolution method: FSC 0.143 CUT-OFF |
-Atomic model buiding
Output model |
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