National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R01AI087946
United States
National Institutes of Health/National Institute of General Medical Sciences
R01GM107629
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R01AI132818
United States
Citation
Journal: Elife / Year: 2019 Title: Structural insights into flagellar stator-rotor interactions. Authors: Yunjie Chang / Ki Hwan Moon / Xiaowei Zhao / Steven J Norris / Md A Motaleb / Jun Liu / Abstract: The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator-rotor interaction, coupled with an ion flux through ...The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator-rotor interaction, coupled with an ion flux through the torque-generating stator. Here we employed cryo-electron tomography to visualize the intact flagellar motor in the Lyme disease spirochete, . By analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the stator complex in situ, but also revealed the stator-rotor interaction at an unprecedented detail. Importantly, the stator-rotor interaction induces a conformational change in the flagella C-ring. Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate the similar conformational change, we propose that the proton-driven torque is responsible for the conformational change required for flagellar rotation.
History
Deposition
Feb 8, 2019
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Header (metadata) release
Mar 27, 2019
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Map release
Sep 25, 2019
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Update
Sep 25, 2019
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Current status
Sep 25, 2019
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
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