Journal: J Struct Biol / Year: 2010 Title: Distinct in situ structures of the Borrelia flagellar motor. Authors: Mikhail Kudryashev / Marek Cyrklaff / Reinhard Wallich / Wolfgang Baumeister / Friedrich Frischknecht / Abstract: Bacteria can be propelled in liquids by flagellar filaments that are attached to and moved by flagellar motors. These motors are rotary nanomachines that use the electrochemical potential from ion ...Bacteria can be propelled in liquids by flagellar filaments that are attached to and moved by flagellar motors. These motors are rotary nanomachines that use the electrochemical potential from ion gradients. The motor can spin in both directions with specific proteins regulating the direction in response to chemotactic stimuli. Here we investigated the structure of flagellar motors of Borrelia spirochetes, the causative agents of Lyme disease in humans. We revealed the structure of the motor complex at 4.6-nm resolution by sub-volume averaging of cryo-electron tomograms and subsequently imposing rotational symmetry. This allowed direct visualisation of individual motor components, the connection between the stator and the peptidoglycan as well as filamentous linkers between the stator and the rod. Two different motor assemblies seem to co-exist at a single bacterial pole. While most motors were completely assembled, a smaller fraction appeared to lack part of the C-ring, which plays a role in protein export and switching the directionality of rotation. Our data suggest a novel mechanism that bacteria may use to control the direction of movement.
History
Deposition
Aug 19, 2009
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Header (metadata) release
Aug 20, 2009
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Map release
Aug 20, 2009
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Update
Oct 10, 2012
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Current status
Oct 10, 2012
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Specimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN / Tilt series - Axis1 - Min angle: -60 ° / Tilt series - Axis1 - Max angle: 60 °
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Image processing
Final reconstruction
Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 46.0 Å / Resolution method: OTHER / Software - Name: EM-package, TOM for matlab Details: The final map is calculated from 107 single motors, 16 fold rotational symmetry applied
CTF correction
Details: no
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