- EMDB-9123: Flagellar motor averaged map from Borrelia burdorferi reveals the... -
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Basic information
Entry
Database: EMDB / ID: EMD-9123
Title
Flagellar motor averaged map from Borrelia burdorferi reveals the cytoplasmic ATPase complex
Map data
A class average shows the 16-fold symmetry at collar and stator region, 23-fold symmetry of the spoke linkers, and 6-fold symmetry of the ATPase.
Sample
Organelle or cellular component: Borrelia burgdorferi flagellar motor averaged structure shows 16-fold symmetry at collar and stators region, 23 spoke linkers, and 6-fold symmetry of the ATPase.
Biological species
Borrelia burgdorferi (Lyme disease spirochete)
Method
subtomogram averaging / cryo EM / Resolution: 33.0 Å
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R01AI087949
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R01DE023080
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R01AI078958
United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases
R01AR060834
United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases
R21AI113014
United States
Welch Foundation
AU-1714
United States
Citation
Journal: PLoS Biol / Year: 2018 Title: Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex. Authors: Zhuan Qin / Jiagang Tu / Tao Lin / Steven J Norris / Chunhao Li / Md A Motaleb / Jun Liu / Abstract: Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export apparatus and a ...Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export apparatus and a cytosolic ATPase complex is responsible for the assembly of the periplasmic flagella. Here, we deployed cryo-electron tomography (cryo-ET) to visualize the fT3SS machine in the Lyme disease spirochete Borrelia burgdorferi. We show, for the first time, that the cytosolic ATPase complex is attached to the flagellar C-ring through multiple spokes to form the "spoke and hub" structure in B. burgdorferi. This structure not only strengthens structural rigidity of the round-shaped C-ring but also appears to rotate with the C-ring. Our studies provide structural insights into the unique mechanisms underlying assembly and rotation of the periplasmic flagella and may provide the basis for the development of novel therapeutic strategies against several pathogenic spirochetes.
History
Deposition
Sep 18, 2018
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Header (metadata) release
Oct 17, 2018
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Map release
Oct 17, 2018
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Update
Aug 12, 2020
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Current status
Aug 12, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Entire : Borrelia burgdorferi flagellar motor averaged structure shows 16-...
Entire
Name: Borrelia burgdorferi flagellar motor averaged structure shows 16-fold symmetry at collar and stators region, 23 spoke linkers, and 6-fold symmetry of the ATPase.
Components
Organelle or cellular component: Borrelia burgdorferi flagellar motor averaged structure shows 16-fold symmetry at collar and stators region, 23 spoke linkers, and 6-fold symmetry of the ATPase.
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Supramolecule #1: Borrelia burgdorferi flagellar motor averaged structure shows 16-...
Supramolecule
Name: Borrelia burgdorferi flagellar motor averaged structure shows 16-fold symmetry at collar and stators region, 23 spoke linkers, and 6-fold symmetry of the ATPase. type: organelle_or_cellular_component / ID: 1 / Parent: 0 Details: Wild-type, asymmetric in situ structure. It is one of the class averages.
Source (natural)
Organism: Borrelia burgdorferi (Lyme disease spirochete) / Strain: B31 / Organelle: Flagellar motor / Location in cell: Cell tip
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Experimental details
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Structure determination
Method
cryo EM
Processing
subtomogram averaging
Aggregation state
cell
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Sample preparation
Buffer
pH: 7 Details: BSK-II liquid medium supplemented with 6% rabbit serum
Grid
Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
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