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- EMDB-0307: Representative tomogram of the COPII in vitro reconstitution used... -

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Basic information

Entry
Database: EMDB / ID: 0307
TitleRepresentative tomogram of the COPII in vitro reconstitution used for subtomogram averaging
Map data*** This is a dummy file *** Representative unbinned 3D-CTF corrected tomogram used for subtomogram averaging of COPII assembled on membranes.
SampleIn vitro reconstitution of COPII membrane deformation with giant unilamellar vesicles (GUVs).:
SourceSaccharomyces cerevisiae (baker's yeast)
Methodelectron tomography / cryo EM
AuthorsZanetti G / Hutchings J / Hagen WJH
CitationJournal: To Be Published
Title: Representative tomogram of the COPII in vitro reconstitution used for subtomogram averaging
Authors: Zanetti G / Hutchings J
DateDeposition: Oct 19, 2018 / Header (metadata) release: Nov 7, 2018 / Map release: Nov 7, 2018 / Last update: Nov 7, 2018

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Structure visualization

Supplemental images

Downloads & links

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Map

Fileemd_0307.map.gz (map file in CCP4 format, 5 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1666 pix
1.33 Å/pix.
= 2210.782 Å
3708 pix
1.33 Å/pix.
= 4920.516 Å
3708 pix
1.33 Å/pix.
= 4920.516 Å

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.327 Å
Density
Minimum - Maximum0.0 - 1.0
Average (Standard dev.)0.999 (0.03160696)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions101010
Origin0.00.00.0
Limit9.09.09.0
Spacing101010
CellA=B=C: 13.27 Å
α=β=γ: 90.0 deg.

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Supplemental data

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Sample components

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Entire In vitro reconstitution of COPII membrane deformation with giant ...

EntireName: In vitro reconstitution of COPII membrane deformation with giant unilamellar vesicles (GUVs).
Number of components: 1

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Component #1: protein, In vitro reconstitution of COPII membrane deformation wi...

ProteinName: In vitro reconstitution of COPII membrane deformation with giant unilamellar vesicles (GUVs).
Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)
Source (engineered)Expression System: Spodoptera frugiperda (fall armyworm)

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Experimental details

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Sample preparation

SpecimenSpecimen state: helical array / Method: cryo EM
Sample solutionpH: 6.8
VitrificationInstrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 3.6 e/Å2 / Illumination mode: OTHER
LensMagnification: 105000.0 X (nominal) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1.5 - 3.5 nm
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: electron tomography / Number of sections: 40

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