SASDF73: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain (Bruton's tyrosi...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDF73

試料

Bruton's Tyrosine Kinase - SH3-SH2-kinase domain

• Bruton's tyrosine kinase - Src homology 3-2 kinase domain (protein), hBTK - SH3-SH2-KD, Homo sapiens

機能・相同性

分子機能:

regulation of B cell cytokine production / proteoglycan catabolic process / monocyte proliferation / positive regulation of interleukin-17A production / regulation of B cell apoptotic process / eosinophil homeostasis / positive regulation of type III hypersensitivity / B cell affinity maturation / positive regulation of synoviocyte proliferation / histamine secretion by mast cell / neutrophil homeostasis / cellular response to molecule of fungal origin / positive regulation of type I hypersensitivity / MyD88 deficiency (TLR2/4) / cellular response to interleukin-7 / MyD88-dependent toll-like receptor signaling pathway / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / positive regulation of immunoglobulin production / positive regulation of B cell differentiation / positive regulation of NLRP3 inflammasome complex assembly / phospholipase activator activity / negative regulation of interleukin-10 production / negative regulation of B cell proliferation / mesoderm development / Fc-epsilon receptor signaling pathway / B cell activation / phosphatidylinositol-3,4,5-trisphosphate binding / phospholipase binding / RHO GTPases Activate WASPs and WAVEs / positive regulation of phagocytosis / positive regulation of B cell proliferation / cell maturation / FCERI mediated Ca+2 mobilization / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / apoptotic signaling pathway / non-specific protein-tyrosine kinase / FCGR3A-mediated phagocytosis / B cell receptor signaling pathway / non-membrane spanning protein tyrosine kinase activity / calcium-mediated signaling / Regulation of actin dynamics for phagocytic cup formation / peptidyl-tyrosine phosphorylation / G beta:gamma signalling through BTK / positive regulation of interleukin-6 production / cellular response to reactive oxygen species / positive regulation of tumor necrosis factor production / G alpha (12/13) signalling events / DAP12 signaling / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / ER-Phagosome pathway / cytoplasmic vesicle / protein tyrosine kinase activity / G alpha (q) signalling events / adaptive immune response / response to lipopolysaccharide / Potential therapeutics for SARS / intracellular signal transduction / protein phosphorylation / membrane raft / innate immune response / perinuclear region of cytoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm

ドメイン・相同性:

Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / : / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / PH-like domain superfamily / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily

構成要素:

Tyrosine-protein kinase BTK

• 生物種: Homo sapiens (ヒト)

登録者

• Daniel Duarte (EPFL)

モデル

• モデル #2789: ; タイプ: dummy / ダミー原子の半径: 2.10 A / カイ2乗値: 0.937 / P-value: 0.139358; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #2818: ; タイプ: atomic / カイ2乗値: 1.559; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain / 試料濃度: 4.32 mg/ml
• バッファ: 名称: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol / pH: 7.5

要素 #1454

名称: hBTK - SH3-SH2-KD / タイプ: protein
記述: Bruton's tyrosine kinase - Src homology 3-2 kinase domain
分子量: 52.171 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q06187
配列:

GAMGSMSELK KVVALYDYMP MNANDLQLRK GDEYFILEES NLPWWRARDK NGQEGYIPSN YVTEAEDSIE MYEWYSKHMT RSQAEQLLKQ EGKEGGFIVR DSSKAGKYTV SVFAKSTGDP QGVIRHYVVC STPQSQYYLA EKHLFSTIPE LINYHQHNSA GLISRLKYPV SQQNKNAPST AGLGYGSWEI DPKDLTFLKE LGTGQFGVVK YGKWRGQYDV AIKMIKEGSM SEDEFIEEAK VMMNLSHEKL VQLYGVCTKQ RPIFIITEYM ANGCLLNYLR EMRHRFQTQQ LLEMCKDVCE AMEYLESKQF LHRDLAARNC LVNDQGVVKV SDFGLSRYVL DDEYTSSVGS KFPVRWSPPE VLMYSKFSSK SDIWAFGVLM WEIYSLGKMP YERFTNSETA EHIAQGLRLY RPHLASEKVY TIMYSCWHEK ADERPTFKIL LSNILDVMDE ES

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.09919 Å / スペクトロメータ・検出器間距離: 2.869 mm
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain
測定日: 2018年11月2日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0401	4.9462

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 623 /

	Min	Max
Q	0.115485	3.04692
P(R) point	1	623
R	0	8.3

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	45 kDa	45 kDa
体積	-	72.26 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	44.18	44.28	0.07
慣性半径, Rg	2.62 nm	2.62 nm	0.01

	Min	Max
D	-	8.3
Guinier point	18	98