SASDF73: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain (Bruton's tyrosi...

Basic information

• Entry: Database: SASBDB / ID: SASDF73

Sample

Bruton's Tyrosine Kinase - SH3-SH2-kinase domain

• Bruton's tyrosine kinase - Src homology 3-2 kinase domain (protein), hBTK - SH3-SH2-KD, Homo sapiens

Function / homology

Function:

regulation of B cell cytokine production / proteoglycan catabolic process / monocyte proliferation / positive regulation of interleukin-17A production / regulation of B cell apoptotic process / eosinophil homeostasis / positive regulation of type III hypersensitivity / B cell affinity maturation / positive regulation of synoviocyte proliferation / histamine secretion by mast cell / neutrophil homeostasis / cellular response to molecule of fungal origin / positive regulation of type I hypersensitivity / MyD88 deficiency (TLR2/4) / cellular response to interleukin-7 / MyD88-dependent toll-like receptor signaling pathway / IRAK4 deficiency (TLR2/4) / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / positive regulation of immunoglobulin production / positive regulation of B cell differentiation / positive regulation of NLRP3 inflammasome complex assembly / phospholipase activator activity / negative regulation of interleukin-10 production / negative regulation of B cell proliferation / mesoderm development / Fc-epsilon receptor signaling pathway / B cell activation / phosphatidylinositol-3,4,5-trisphosphate binding / phospholipase binding / RHO GTPases Activate WASPs and WAVEs / positive regulation of phagocytosis / positive regulation of B cell proliferation / cell maturation / FCERI mediated Ca+2 mobilization / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / apoptotic signaling pathway / non-specific protein-tyrosine kinase / FCGR3A-mediated phagocytosis / B cell receptor signaling pathway / non-membrane spanning protein tyrosine kinase activity / calcium-mediated signaling / Regulation of actin dynamics for phagocytic cup formation / peptidyl-tyrosine phosphorylation / G beta:gamma signalling through BTK / positive regulation of interleukin-6 production / cellular response to reactive oxygen species / positive regulation of tumor necrosis factor production / G alpha (12/13) signalling events / DAP12 signaling / positive regulation of NF-kappaB transcription factor activity / T cell receptor signaling pathway / ER-Phagosome pathway / cytoplasmic vesicle / protein tyrosine kinase activity / G alpha (q) signalling events / adaptive immune response / response to lipopolysaccharide / Potential therapeutics for SARS / intracellular signal transduction / protein phosphorylation / membrane raft / innate immune response / perinuclear region of cytoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm

Domain/homology:

Tyrosine-protein kinase BTK, SH3 domain / Zinc finger, Btk motif / BTK motif / Zinc finger Btk-type profile. / Bruton's tyrosine kinase Cys-rich motif / : / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / PH-like domain superfamily / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily

Component:

Tyrosine-protein kinase BTK

• Biological species: Homo sapiens (human)

Contact author

• Daniel Duarte (EPFL)

Models

• Model #2789: ; Type: dummy / Radius of dummy atoms: 2.10 A / Chi-square value: 0.937 / P-value: 0.139358; Search similar-shape structures of this assembly by Omokage search (details)
• Model #2818: ; Type: atomic / Chi-square value: 1.559; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain / Specimen concentration: 4.32 mg/ml
• Buffer: Name: 20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol / pH: 7.5

Entity #1454

Name: hBTK - SH3-SH2-KD / Type: protein
Description: Bruton's tyrosine kinase - Src homology 3-2 kinase domain
Formula weight: 52.171 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q06187
Sequence:

GAMGSMSELK KVVALYDYMP MNANDLQLRK GDEYFILEES NLPWWRARDK NGQEGYIPSN YVTEAEDSIE MYEWYSKHMT RSQAEQLLKQ EGKEGGFIVR DSSKAGKYTV SVFAKSTGDP QGVIRHYVVC STPQSQYYLA EKHLFSTIPE LINYHQHNSA GLISRLKYPV SQQNKNAPST AGLGYGSWEI DPKDLTFLKE LGTGQFGVVK YGKWRGQYDV AIKMIKEGSM SEDEFIEEAK VMMNLSHEKL VQLYGVCTKQ RPIFIITEYM ANGCLLNYLR EMRHRFQTQQ LLEMCKDVCE AMEYLESKQF LHRDLAARNC LVNDQGVVKV SDFGLSRYVL DDEYTSSVGS KFPVRWSPPE VLMYSKFSSK SDIWAFGVLM WEIYSLGKMP YERFTNSETA EHIAQGLRLY RPHLASEKVY TIMYSCWHEK ADERPTFKIL LSNILDVMDE ES

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.869 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Bruton's Tyrosine Kinase - SH3-SH2-kinase domain / Measurement date: Nov 2, 2018 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /

	Min	Max
Q	0.0401	4.9462

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 623 /

	Min	Max
Q	0.115485	3.04692
P(R) point	1	623
R	0	8.3

Result

Type of curve: single_conc /

	Experimental	Porod
MW	45 kDa	45 kDa
Volume	-	72.26 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	44.18	44.28	0.07
Radius of gyration, Rg	2.62 nm	2.62 nm	0.01

	Min	Max
D	-	8.3
Guinier point	18	98