SASDER6: Glucosamine kinase from Streptacidiphilus jiangxiensis in presenc...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDER6

試料

Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 50 mM D-glucose and 1 mM ATP

• Glucosamine kinase (protein), Streptacidiphilus jiangxiensis

• 機能・相同性: glucosamine kinase / Glucosamine kinase / glucosamine kinase activity / carbohydrate metabolic process / phosphorylation / Protein kinase-like domain superfamily / magnesium ion binding / ATP binding / Glucosamine kinase
• 生物種: Streptacidiphilus jiangxiensis (バクテリア)
• 引用: ジャーナル: mBio / 年: 2019; タイトル: Molecular Fingerprints for a Novel Enzyme Family in with Glucosamine Kinase Activity.; 著者: José A Manso / Daniela Nunes-Costa / Sandra Macedo-Ribeiro / Nuno Empadinhas / Pedro José Barbosa Pereira / ; 要旨: have long been the main source of antibiotics, secondary metabolites with tightly controlled biosynthesis by environmental and physiological factors. Phosphorylation of exogenous glucosamine has been suggested as a mechanism for incorporation of this extracellular material into secondary metabolite biosynthesis, but experimental evidence of specific glucosamine kinases in is lacking. Here, we present the molecular fingerprints for the identification of a unique family of actinobacterial glucosamine kinases. Structural and biochemical studies on a distinctive kinase from the soil bacterium unveiled its preference for glucosamine and provided structural evidence of a phosphoryl transfer to this substrate. Conservation of glucosamine-contacting residues across a large number of uncharacterized actinobacterial proteins unveiled a specific glucosamine binding sequence motif. This family of kinases and their genetic context may represent the missing link for the incorporation of environmental glucosamine into the antibiotic biosynthesis pathways in and can be explored to enhance antibiotic production. The discovery of novel enzymes involved in antibiotic biosynthesis pathways is currently a topic of utmost importance. The high levels of antibiotic resistance detected worldwide threaten our ability to combat infections and other 20th-century medical achievements, namely, organ transplantation or cancer chemotherapy. We have identified and characterized a unique family of enzymes capable of phosphorylating glucosamine to glucosamine-6-phosphate, a crucial molecule directly involved in the activation of antibiotic production pathways in , nature's main source of antimicrobials. The consensus sequence identified for these glucosamine kinases will help establish a molecular fingerprint to reveal yet-uncharacterized sequences in antibiotic producers, which should have an important impact in biotechnological and biomedical applications, including the enhancement and optimization of antibiotic production.

登録者

• Jose A Manso (Instituto de Investigaçao e Inovaçao em Saúde (i3S-IBMC), Universidade do Porto)

モデル

• モデル #2526: ; タイプ: atomic; コメント: Volume fraction for this structure was of 0.56 as determined OLIGOMER; カイ2乗値: 14.42; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #2527: ; タイプ: atomic; コメント: Volume fraction for this structure was of 0.44 as determined OLIGOMER; カイ2乗値: 14.42; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 50 mM D-glucose and 1 mM ATP; 試料濃度: 1.25-10.00
• バッファ: 名称: 20 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl2, 5 mM DTT, 50 mM D-glucose, 1 mM ATP; pH: 8

要素 #1337

タイプ: protein / 記述: Glucosamine kinase / 分子量: 48.377 / 分子数: 1 / 由来: Streptacidiphilus jiangxiensis / 参照: UniProt: A0A1H7TQR5
配列:

MTPNWSELVA AADPALVLPS GERRAEVAVP GPLRLDALLD LGEGHAVGVV RSADAARWTV PLVRDGAGGV RRSRPGDGTA EHLVAALARR GATPDAAFVL EAFTGAAPVT GERGIIVDQT NESVIVGECA VVKWAVRLPA EGEPGSPAAQ RIAALARGGF TEMPRPWGLL TLAEGAQPVL LASVVAYLPG ALDGWDWAVD DVRRLARGEL TMDQALLPAA QLGTLTARMH AALAARGRTP ATAADVAAWG VRMREELDEA VASVPGAEGE RLKAWAPRIA DVYAELDALA GTPLIDVHGD FHVGQILRAD GRYAVVDFDG NPVLPADQRA ARQPAALDVV GMTASLDHVG RVVVFRTPDV DPAPVRAWIA AAQRSFLDAY RTTLARLDAD DLFDDRLLTP LRYAQEVREY LYAVRHLPHW VYVPDLSLTD LLPERLKDKK LAAALEHHHH HH

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.09919 Å / スペクトロメータ・検出器間距離: 2.867 mm
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Glucosamine kinase from Streptacidiphilus jiangxiensis in presence of 50 mM D-glucose and 1 mM ATP
測定日: 2016年11月24日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0442	4.9439

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 630 /

	Min	Max
Q	0.0442167	3.0076
P(R) point	1	630
R	0	7.7

結果

カーブのタイプ: extrapolated

	実験値	Standard	Porod
分子量	41.7 kDa	41.7 kDa	45.8 kDa
体積	-	-	73.29 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	36.97	36.86	0.02
慣性半径, Rg	2.493 nm	2.48 nm	0.01

	Min	Max
D	-	7.7
Guinier point	33	97