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- SASDDM7: Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and ... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDDM7
SampleComplex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5
  • anti-DISC1 single-domain camelid antibody VHH B5 (protein), VHH B5
  • Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 (protein), DISC1 12D2
Function / homology
Function and homology information


pyramidal neuron migration to cerebral cortex / cell proliferation in forebrain / central region of growth cone / mitochondrial calcium ion homeostasis / regulation of dendritic spine development / regulation of synapse maturation / intermediate filament cytoskeleton / non-motile cilium assembly / positive regulation of ubiquitin-dependent protein catabolic process / dynein complex ...pyramidal neuron migration to cerebral cortex / cell proliferation in forebrain / central region of growth cone / mitochondrial calcium ion homeostasis / regulation of dendritic spine development / regulation of synapse maturation / intermediate filament cytoskeleton / non-motile cilium assembly / positive regulation of ubiquitin-dependent protein catabolic process / dynein complex / protein localization to centrosome / regulation of postsynapse organization / kinesin complex / positive regulation of cell-matrix adhesion / positive regulation of neuroblast proliferation / ciliary base / detection of temperature stimulus involved in sensory perception of pain / TOR signaling / microtubule organizing center / response to electrical stimulus / cilium assembly / kinesin binding / positive regulation of Wnt signaling pathway / canonical Wnt signaling pathway / positive regulation of axon extension / regulation of synaptic transmission, glutamatergic / positive regulation of neuron projection development / neuron cellular homeostasis / GABA-ergic synapse / microtubule cytoskeleton organization / neuron migration / synaptic vesicle / presynapse / cell body / ubiquitin-dependent protein catabolic process / molecular adaptor activity / microtubule / postsynaptic density / ciliary basal body / centrosome / protein-containing complex binding / perinuclear region of cytoplasm / glutamatergic synapse / mitochondrion / identical protein binding / cytosol
Similarity search - Function
Disrupted in schizophrenia 1
Similarity search - Domain/homology
Disrupted in schizophrenia 1 protein
Similarity search - Component
CitationDate: 2018 Jan 11
Title: Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein
Authors: Yerabham A / Müller-Schiffmann A / Ziehm T / Stadler A / Köber S / Indurkhya X / Marreiros R / Trossbach S / Bradshaw N / Prikulis I / Willbold D / Weiergräber O
Contact author
  • Andreas Stadler (Forschungszentrum Jülich)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2019
Type: dummy / Software: (2.8.3) / Radius of dummy atoms: 2.20 A / Chi-square value: 0.652 / P-value: 0.000475
Search similar-shape structures of this assembly by Omokage search (details)
Model #2020
Type: dummy / Software: (2.8.3) / Radius of dummy atoms: 2.75 A / Chi-square value: 0.652 / P-value: 0.000475
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5
Specimen concentration: 2.35 mg/ml / Entity id: 1073 / 1077
BufferName: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT / pH: 7.4
Entity #1073Name: VHH B5 / Type: protein
Description: anti-DISC1 single-domain camelid antibody VHH B5
Formula weight: 13.924 / Num. of mol.: 1
Sequence:
MAQVKLEESG GGLVQAGGSL RLSCAASGTL FSISVMGWYR QAPGMQRELV GSITDGGSTI YGDSVKGRFA ISKDNAKRTI YLQMNSLKPE DTAVYYCNYR RLTSNYWGQG TQVTVSSAAA LEHHHHHH
Entity #1077Name: DISC1 12D2 / Type: protein
Description: Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836
Formula weight: 19.426 / Num. of mol.: 1 / References: UniProt: Q9NRI5
Sequence:
MGHHHHHHDY DIPTTENLYF QGWEADLEAC RLLIQSLQLQ EARGSLSVED ERQMDDLEGA APPIPPRLHS EDKRKTPLKV LEEWKTHLIP SLHCAGGEQK EESYILSAEL GEKCEDIGKK LLYLEDQLHT AIHSHDEDLI QSLRRELQMV KETLQAMILQ LQPAKEAGSG

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å
DetectorName: Pilatus 1M
Scan
Title: Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5
Measurement date: Feb 19, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.08 4.9448
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 516 /
MinMax
Q0.211998 2.63966
P(R) point1 516
R0 10.35
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW36.2 kDa36.2 kDa45.2 kDa
Volume--76.8 nm3

P(R)GuinierGuinier error
Forward scattering, I068.35 68.06 0.08
Radius of gyration, Rg3.146 nm3.07 nm0.02

MinMax
D-10.35
Guinier point29 73

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