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- SASDDL7: anti-DISC1 single-domain camelid antibody VHH B5 (VHH B5) -

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Basic information

Entry
Database: SASBDB / ID: SASDDL7
Sampleanti-DISC1 single-domain camelid antibody VHH B5
  • anti-DISC1 single-domain camelid antibody VHH B5 (protein), VHH B5
CitationDate: 2018 Jan 11
Title: Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein
Authors: Yerabham A / Müller-Schiffmann A / Ziehm T / Stadler A / Köber S / Indurkhya X / Marreiros R / Trossbach S / Bradshaw N / Prikulis I / Willbold D / Weiergräber O
Contact author
  • Andreas Stadler (Forschungszentrum Jülich)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2015
Type: dummy / Radius of dummy atoms: 1.90 A / Chi-square value: 33.20
Search similar-shape structures of this assembly by Omokage search (details)
Model #2016
Type: dummy / Radius of dummy atoms: 1.75 A / Chi-square value: 33.20
Search similar-shape structures of this assembly by Omokage search (details)
Model #2017
Type: dummy / Software: (2.8.3) / Radius of dummy atoms: 1.80 A / Chi-square value: 0.744 / P-value: 0.037400
Search similar-shape structures of this assembly by Omokage search (details)
Model #2018
Type: dummy / Software: (2.8.3) / Radius of dummy atoms: 1.75 A / Chi-square value: 0.744 / P-value: 0.037400
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: anti-DISC1 single-domain camelid antibody VHH B5 / Specimen concentration: 2.80-5.60
BufferName: 25 mM Tris, 150 mM NaCl / pH: 7.4
Entity #1073Name: VHH B5 / Type: protein
Description: anti-DISC1 single-domain camelid antibody VHH B5
Formula weight: 13.924 / Num. of mol.: 1
Sequence:
MAQVKLEESG GGLVQAGGSL RLSCAASGTL FSISVMGWYR QAPGMQRELV GSITDGGSTI YGDSVKGRFA ISKDNAKRTI YLQMNSLKPE DTAVYYCNYR RLTSNYWGQG TQVTVSSAAA LEHHHHHH

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å
DetectorName: Pilatus 1M
Scan
Title: anti-DISC1 single-domain camelid antibody VHH B5 / Measurement date: Feb 19, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0753 4.9448
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 636 /
MinMax
Q0.268565 3.26189
P(R) point1 636
R0 8.39
Result
Type of curve: merged
Comments: The data was obtained from the merged SAXS profiles measured from two samples at 2.8 and 5.6 mg/mL.
ExperimentalStandardStandard errorPorod
MW15.15 kDa15.15 kDa0.017 12.5 kDa
Volume---21.3 nm3

P(R)GuinierGuinier error
Forward scattering, I015.28 15.15 0.017
Radius of gyration, Rg1.907 nm1.81 nm0.02

MinMax
D-8.39
Guinier point42 118

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