SASDDD9: Cardiac myosin binding protein C: tri-helix bundle from the motif...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDDD9

試料

Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain

• cardiac myosin binding protein C: tri-helix bundle-C2 (protein), tri-helix bundle-C2, human sequence obtained using E. coli expression

機能・相同性

分子機能:

C zone / regulation of muscle filament sliding / striated muscle myosin thick filament / regulation of striated muscle contraction / cardiac myofibril / positive regulation of ATP-dependent activity / Striated Muscle Contraction / M band / A band / structural constituent of muscle / sarcomere organization / ventricular cardiac muscle tissue morphogenesis / myosin heavy chain binding / myosin binding / ATPase activator activity / heart morphogenesis / cardiac muscle contraction / titin binding / sarcomere / actin binding / cell adhesion / metal ion binding / identical protein binding / cytosol

ドメイン・相同性:

: / MyBP-C, tri-helix bundle domain / Tri-helix bundle domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold

構成要素:

Myosin-binding protein C, cardiac-type

• 生物種: human sequence obtained using E. coli expression
• 引用: ジャーナル: Structure / 年: 2016; タイトル: A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C.; 著者: Katharine A Michie / Ann H Kwan / Chang-Shung Tung / J Mitchell Guss / Jill Trewhella / ; 要旨: The nuclear magnetic resonance (NMR) structure of the tri-helix bundle (THB) of the m-domain plus C2 (ΔmC2) of myosin-binding protein C (MyBP-C) has revealed a highly flexible seven-residue linker between the structured THB and C2. Bioinformatics shows significant patterns of conservation across the THB-linker sequence, with the linker containing a strictly conserved serine in all MyBP-C isoforms. Clinically linked mutations further support the functional significance of the THB-linker region. NMR, small-angle X-ray scattering, and binding studies show the THB-linker plus the first ten residues of C2 undergo dramatic changes when ΔmC2 binds Ca-calmodulin, with the linker and C2 N-terminal residues contributing significantly to the affinity. Modeling of all available experimental data indicates that the THB tertiary structure must be disrupted to form the complex. These results are discussed in the context of the THB-linker and the N-terminal residues of C2 forming a polymorphic binding domain that could accommodate multiple binding partners in the dynamic sarcomere.

登録者

• Jill Trewhella (School of Molecular Bioscience, University of Sydney., Sydney, New South Wales 2006, Australia)

モデル

• モデル #2155: ; タイプ: atomic; コメント: one state of 2-state MultiFoXS model, weight 0.753; カイ2乗値: 3.56671154143 / P-value: 0.820000; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #2156: ; タイプ: atomic; コメント: one state of 2-state MultiFoXS model, weight 0.247; カイ2乗値: 3.56671154143 / P-value: 0.820000; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain; 試料濃度: 0.98-4.89
• バッファ: 名称: 150 mM NaCl, 10 mM MES, 2 mM TCEP, 1 mM NaN3 at 4°C; pH: 6.5

要素 #1178

名称: tri-helix bundle-C2 / タイプ: protein
記述: cardiac myosin binding protein C: tri-helix bundle-C2
分子量: 15.474 / 分子数: 1 / 由来: human sequence obtained using E. coli expression / 参照: UniProt: Q14896
配列:

GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKE

実験情報

• ビーム: 設備名称: Australian Synchrotron SAXS/WAXS / 地域: Melbourne / 国: Australia / 形状: Point / 線源: X-ray synchrotron / 波長: 0.10332 Å / スペクトロメータ・検出器間距離: 2.68024 mm
• 検出器: 名称: Pilatus 1M / Pixsize x: 172 mm

スキャン

タイトル: Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain
測定日: 2015年4月18日 / セル温度: 22 °C / 照射時間: 1 sec. / フレーム数: 26 / 単位: 1/A /

	Min	Max
Q	0.0096	0.3436

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 733 /

	Min	Max
Q	0.0161956	0.301584
P(R) point	1	733
R	0	80

結果

カーブのタイプ: extrapolated
コメント: The concentration series SAXS data and EOM models are provided in the full entry zip archive.

	実験値	Standard	Porod
分子量	15.475 kDa	17.896 kDa	13.067 kDa
体積	-	-	19.6 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.04606	5.0E-5	0.046	0.0001
慣性半径, Rg	1.99 nm	0.05	1.91 nm	0.08

	Min	Max
D	-	8
Guinier point	18	146