SASDCH7: N-terminal domain of DsbD from N. meningitidis (N-terminus of dis...

Basic information

• Entry: Database: SASBDB / ID: SASDCH7

Sample

N-terminal domain of DsbD from N. meningitidis

• N-terminus of disulfide interchange protein DsbD (protein), n-DsbD, Neisseria meningitidis

Function / homology

Function:

protein-disulfide reductase / protein-disulfide reductase [NAD(P)H] activity / cytochrome complex assembly / electron transfer activity / plasma membrane

Domain/homology:

Thiol:disulphide interchange protein DsbD / Thiol:disulfide interchange protein DsbD, N-terminal domain superfamily / DsbD gamma / Thiol:disulfide interchange protein DsbD, N-terminal domain / Thiol:disulfide interchange protein DsbD, N-terminal / Cytochrome C biogenesis protein, transmembrane domain / Cytochrome C biogenesis protein transmembrane domain / Thioredoxin-like domain / Thioredoxin-like fold / Thioredoxin domain profile. / Thioredoxin domain / Thioredoxin-like superfamily

Component:

Thiol:disulfide interchange protein DsbD

• Biological species: Neisseria meningitidis (bacteria)
• Citation: Date: 2018 Jan 1; Title: Production, biophysical characterization and initial crystallization studies of the N- and C-terminal domains of DsbD, an essential enzyme in Neisseria meningitidis; Authors: Smith R / Whitten A / Paxman J / Kahler C / Scanlon M

Contact author

• Andrew Whitten (ANSTO, Australian Nuclear Science and Technology Organisation, Kirrawee DC, NSW 2232, Australia)

Models

• Model #1528: ; Type: dummy / Software: (5.3) / Radius of dummy atoms: 1.50 A / Chi-square value: 1.899; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: N-terminal domain of DsbD from N. meningitidis / Specimen concentration: 0.40-5.90
• Buffer: Name: 25mM HEPES 150mM NaCl / pH: 6.7

Entity #792

Name: n-DsbD / Type: protein
Description: N-terminus of disulfide interchange protein DsbD
Formula weight: 13.911 / Num. of mol.: 1 / Source: Neisseria meningitidis / References: UniProt: Q9JTL9
Sequence:

SNANDLLPPE KAFVPELAVA DDGVNVRFRI ADGYYMYQAK IVGKTDPADL LGQPSFSKGE EKEDEFFGRQ TVYHHEAQVA FPYAKAVGEP YKLVLTYQGC AEVGVCYPPV DTEFDISGNG TYHPQT

Experimental information

• Beam: Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 1.48 mm
• Detector: Name: Pilatus 1M

Scan

Title: N-terminal domain of DsbD from N. meningitidis / Measurement date: May 4, 2013 / Storage temperature: 4 °C / Cell temperature: 12 °C / Exposure time: 2 sec. / Number of frames: 30 / Unit: 1/A /

	Min	Max
Q	0.0136	0.399

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 301 /

	Min	Max
Q	0.01101	0.399
P(R) point	2	302
R	0	57

Result

Type of curve: single_conc

	Experimental	Standard	Standard error	Porod
MW	13.2 kDa	13.2 kDa	1	14.2 kDa
Volume	-	-	-	17.3 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.007688	1.2E-5	0.00776	3.0E-5
Radius of gyration, Rg	1.73 nm	0.1	1.76 nm	0.02

	Min	Max	Error
D	-	5.7	0.3
Guinier point	3	37	-