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- SASDCH7: N-terminal domain of DsbD from N. meningitidis (N-terminus of dis... -

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Basic information

Entry
Database: SASBDB / ID: SASDCH7
SampleN-terminal domain of DsbD from N. meningitidis
  • N-terminus of disulfide interchange protein DsbD (protein), n-DsbD, Neisseria meningitidis
Function / homology
Function and homology information


protein-disulfide reductase / protein-disulfide reductase (NAD(P)H) activity / cytochrome complex assembly / membrane => GO:0016020 / electron transfer activity / plasma membrane
Similarity search - Function
Thiol:disulfide interchange protein DsbD, N-terminal domain superfamily / Thiol:disulphide interchange protein DsbD / DsbD gamma / Thiol:disulfide interchange protein DsbD, N-terminal domain / Thiol:disulfide interchange protein DsbD, N-terminal / Cytochrome C biogenesis protein, transmembrane domain / Cytochrome C biogenesis protein transmembrane region / Thioredoxin / Thioredoxin domain profile. / Thioredoxin domain / Thioredoxin-like superfamily
Similarity search - Domain/homology
Thiol:disulfide interchange protein DsbD
Similarity search - Component
Biological speciesNeisseria meningitidis (bacteria)
CitationDate: 2018 Jan 1
Title: Production, biophysical characterization and initial crystallization studies of the N- and C-terminal domains of DsbD, an essential enzyme in Neisseria meningitidis
Authors: Smith R / Whitten A / Paxman J / Kahler C / Scanlon M
Contact author
  • Andrew Whitten (ANSTO, Australian Nuclear Science and Technology Organisation, Kirrawee DC, NSW 2232, Australia)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1528
Type: dummy / Software: (5.3) / Radius of dummy atoms: 1.50 A / Chi-square value: 1.899
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: N-terminal domain of DsbD from N. meningitidis / Specimen concentration: 0.40-5.90
BufferName: 25mM HEPES 150mM NaCl / pH: 6.7
Entity #792Name: n-DsbD / Type: protein
Description: N-terminus of disulfide interchange protein DsbD
Formula weight: 13.911 / Num. of mol.: 1 / Source: Neisseria meningitidis / References: UniProt: Q9JTL9
Sequence:
SNANDLLPPE KAFVPELAVA DDGVNVRFRI ADGYYMYQAK IVGKTDPADL LGQPSFSKGE EKEDEFFGRQ TVYHHEAQVA FPYAKAVGEP YKLVLTYQGC AEVGVCYPPV DTEFDISGNG TYHPQT

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Experimental information

BeamInstrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / : Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 1.48 mm
DetectorName: Pilatus 1M
Scan
Title: N-terminal domain of DsbD from N. meningitidis / Measurement date: May 4, 2013 / Storage temperature: 4 °C / Cell temperature: 12 °C / Exposure time: 2 sec. / Number of frames: 30 / Unit: 1/A /
MinMax
Q0.0136 0.399
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 301 /
MinMax
Q0.01101 0.399
P(R) point2 302
R0 57
Result
Type of curve: single_conc
ExperimentalStandardStandard errorPorod
MW13.2 kDa13.2 kDa1 14.2 kDa
Volume---17.3 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.007688 1.2E-5 0.00776 3.0E-5
Radius of gyration, Rg1.73 nm0.1 1.76 nm0.02

MinMaxError
D-5.7 0.3
Guinier point3 37 -

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