SASDC93: Tetrameric apoptosis regulator BAX (Bcl-2 associated X)

Basic information

• Entry: Database: SASBDB / ID: SASDC93

Sample

Tetrameric apoptosis regulator BAX (Bcl-2 associated X)

• Apoptosis regulator BAX (Bcl-2 associated X) (protein), BAX, Homo sapiens

Function / homology

Function:

T cell homeostatic proliferation / release of matrix enzymes from mitochondria / positive regulation of developmental pigmentation / protein insertion into mitochondrial membrane / BAX complex / B cell receptor apoptotic signaling pathway / positive regulation of reproductive process / positive regulation of motor neuron apoptotic process / regulation of mammary gland epithelial cell proliferation / spermatid differentiation / Activation, translocation and oligomerization of BAX / positive regulation of B cell apoptotic process / development of secondary sexual characteristics / NTRK3 as a dependence receptor / Sertoli cell proliferation / positive regulation of apoptotic DNA fragmentation / B cell homeostatic proliferation / glycosphingolipid metabolic process / positive regulation of mitochondrial membrane permeability involved in apoptotic process / retinal cell programmed cell death / B cell negative selection / BAK complex / activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c / apoptotic process involved in blood vessel morphogenesis / mitochondrial fragmentation involved in apoptotic process / regulation of mitochondrial membrane permeability involved in programmed necrotic cell death / negative regulation of endoplasmic reticulum calcium ion concentration / apoptotic process involved in embryonic digit morphogenesis / mitochondrial permeability transition pore complex / Release of apoptotic factors from the mitochondria / post-embryonic camera-type eye morphogenesis / Transcriptional regulation by RUNX2 / establishment or maintenance of transmembrane electrochemical gradient / apoptotic process involved in mammary gland involution / positive regulation of apoptotic process involved in mammary gland involution / regulation of nitrogen utilization / B cell apoptotic process / endoplasmic reticulum calcium ion homeostasis / positive regulation of endoplasmic reticulum unfolded protein response / fertilization / calcium ion transport into cytosol / motor neuron apoptotic process / mitochondrial fusion / channel activity / positive regulation of epithelial cell apoptotic process / Bcl-2 family protein complex / epithelial cell apoptotic process / myeloid cell homeostasis / positive regulation of calcium ion transport into cytosol / hypothalamus development / thymocyte apoptotic process / pore complex / BH3 domain binding / odontogenesis of dentin-containing tooth / apoptotic mitochondrial changes / germ cell development / positive regulation of IRE1-mediated unfolded protein response / positive regulation of release of cytochrome c from mitochondria / negative regulation of mitochondrial membrane potential / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / vagina development / B cell homeostasis / negative regulation of apoptotic signaling pathway / intrinsic apoptotic signaling pathway by p53 class mediator / extrinsic apoptotic signaling pathway via death domain receptors / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / response to axon injury / ectopic germ cell programmed cell death / blood vessel remodeling / cellular response to unfolded protein / Pyroptosis / supramolecular fiber organization / negative regulation of peptidyl-serine phosphorylation / positive regulation of intrinsic apoptotic signaling pathway / negative regulation of fibroblast proliferation / homeostasis of number of cells within a tissue / ovarian follicle development / extrinsic apoptotic signaling pathway / release of sequestered calcium ion into cytosol / extrinsic apoptotic signaling pathway in absence of ligand / response to salt stress / Hsp70 protein binding / intrinsic apoptotic signaling pathway / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / positive regulation of release of sequestered calcium ion into cytosol / release of cytochrome c from mitochondria / regulation of mitochondrial membrane potential / negative regulation of protein binding / kidney development / apoptotic signaling pathway / response to gamma radiation / positive regulation of protein-containing complex assembly / neuron migration / response to toxic substance / cerebral cortex development / cellular response to virus / activation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of neuron apoptotic process / cellular response to UV / intrinsic apoptotic signaling pathway in response to DNA damage

Domain/homology:

Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl-2 family / Bcl-2, Bcl-2 homology region 1-3 / Bcl2-like / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily

Component:

Apoptosis regulator BAX

• Biological species: Homo sapiens (human)
• Citation: Year: 2017; Title: Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution; Authors: Shih O / Yeh Y / Liao K / Sung T / Chiang Y

Contact author

• Orion Shih (National Synchrotron Radiation Research Center)

Models

• Model #1240: ; Type: mix / Software: SASREF / Radius of dummy atoms: 1.90 A / Chi-square value: 1.25; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Tetrameric apoptosis regulator BAX (Bcl-2 associated X); Specimen concentration: 2 mg/ml
• Buffer: Name: 20mM sodium phosphate 100mM NaCl / pH: 8 / Comment: 20mM sodium phosphate, 100mM NaCl

Entity #669

Name: BAX / Type: protein / Description: Apoptosis regulator BAX (Bcl-2 associated X) / Formula weight: 21.184 / Num. of mol.: 4 / Source: Homo sapiens / References: UniProt: Q07812
Sequence:

MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG

Experimental information

• Beam: Instrument name: Taiwan Photon Source 23A / City: NSRRC, Hsinchu / 国: Taiwan / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.08266 Å / Dist. spec. to detc.: 3.17 mm
• Detector: Name: Pilatus 1M-F / Pixsize x: 172 mm

Scan

Title: Tetrameric apoptosis regulator BAX (Bcl-2 associated X)
Measurement date: May 28, 2015 / Cell temperature: 15 °C / Exposure time: 30 sec. / Number of frames: 2 / Unit: 1/A /

	Min	Max
Q	0.0144	0.2991

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 178 /

	Min	Max
Q	0.01769	0.2175
P(R) point	1	178
R	0	119.6

Result

Type of curve: single_conc /

	Experimental	Porod
MW	85.5 kDa	-
Volume	-	180 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.02354	0.0002	0.0228923	0.00032
Radius of gyration, Rg	3.85 nm	0.004	3.645 nm	0.07

	Min	Max
D	-	11.96
Guinier point	5	27