SASDC83: Dimeric apoptosis regulator BAX (Bcl-2 associated X)

Basic information

• Entry: Database: SASBDB / ID: SASDC83

Sample

Dimeric apoptosis regulator BAX (Bcl-2 associated X)

• Apoptosis regulator BAX (Bcl-2 associated X) (protein), BAX, Homo sapiens

Function / homology

Function:

release of matrix enzymes from mitochondria / BAX complex / B cell receptor apoptotic signaling pathway / Activation, translocation and oligomerization of BAX / B cell apoptotic process / positive regulation of apoptotic DNA fragmentation / NTRK3 as a dependence receptor / BAK complex / mitochondrial permeability transition pore complex / Release of apoptotic factors from the mitochondria / mitochondrial fragmentation involved in apoptotic process / Transcriptional regulation by RUNX2 / establishment or maintenance of transmembrane electrochemical gradient / endoplasmic reticulum calcium ion homeostasis / apoptotic mitochondrial changes / mitochondrial fusion / execution phase of apoptosis / Bcl-2 family protein complex / pore complex / extrinsic apoptotic signaling pathway via death domain receptors / positive regulation of IRE1-mediated unfolded protein response / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / positive regulation of release of cytochrome c from mitochondria / negative regulation of mitochondrial membrane potential / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / extrinsic apoptotic signaling pathway in absence of ligand / cellular response to unfolded protein / positive regulation of intrinsic apoptotic signaling pathway / BH3 domain binding / Pyroptosis / negative regulation of protein binding / extrinsic apoptotic signaling pathway / release of cytochrome c from mitochondria / intrinsic apoptotic signaling pathway / supramolecular fiber organization / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / apoptotic signaling pathway / regulation of mitochondrial membrane potential / Hsp70 protein binding / intrinsic apoptotic signaling pathway in response to DNA damage / cellular response to virus / positive regulation of protein-containing complex assembly / response to toxic substance / positive regulation of neuron apoptotic process / nuclear envelope / channel activity / regulation of apoptotic process / mitochondrial outer membrane / positive regulation of apoptotic process / apoptotic process / protein heterodimerization activity / lipid binding / negative regulation of apoptotic process / endoplasmic reticulum membrane / endoplasmic reticulum / protein homodimerization activity / mitochondrion / extracellular exosome / membrane / identical protein binding / nucleus / cytosol / cytoplasm

Domain/homology:

Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / Bcl-2 family / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl2-like / Bcl-2, Bcl-2 homology region 1-3 / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily

Component:

Apoptosis regulator BAX

• Biological species: Homo sapiens (human)
• Citation: Year: 2017; Title: Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution; Authors: Shih O / Yeh Y / Liao K / Sung T / Chiang Y

Contact author

• Orion Shih (National Synchrotron Radiation Research Center)

Models

• Model #1239: ; Type: mix / Software: CORAL / Radius of dummy atoms: 1.90 A / Chi-square value: 1.25; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Dimeric apoptosis regulator BAX (Bcl-2 associated X) / Specimen concentration: 2 mg/ml
• Buffer: Name: 20mM sodium phosphate 100mM NaCl / pH: 8 / Comment: 20mM sodium phosphate, 100mM NaCl

Entity #668

Name: BAX / Type: protein / Description: Apoptosis regulator BAX (Bcl-2 associated X) / Formula weight: 21.184 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q07812
Sequence:

MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG

Experimental information

• Beam: Instrument name: Taiwan Photon Source 23A / City: NSRRC, Hsinchu / 国: Taiwan / Type of source: X-ray synchrotron / Wavelength: 0.08266 Å / Dist. spec. to detc.: 3.17 mm
• Detector: Name: Pilatus 1M-F / Pixsize x: 172 mm

Scan

Title: Dimeric apoptosis regulator BAX (Bcl-2 associated X) / Measurement date: May 28, 2015 / Cell temperature: 15 °C / Exposure time: 30 sec. / Number of frames: 3 / Unit: 1/A /

	Min	Max
Q	0.0144	0.2991

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 199 /

	Min	Max
Q	0.0185	0.27071
P(R) point	1	199
R	0	95.24

Result

Type of curve: single_conc /

	Experimental	Porod
MW	39.5 kDa	-
Volume	-	85.57 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.01585	0.000135	0.01578	0.0002
Radius of gyration, Rg	3.01 nm	0.03	2.97 nm	0.06

	Min	Max
D	-	9.52
Guinier point	6	38