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- PDB-6f3t: Crystal structure of the human TAF5-TAF6-TAF9 complex -

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Basic information

Entry
Database: PDB / ID: 6f3t
TitleCrystal structure of the human TAF5-TAF6-TAF9 complex
Components
  • Transcription initiation factor TFIID subunit 5
  • Transcription initiation factor TFIID subunit 6
  • Transcription initiation factor TFIID subunit 9
KeywordsTRANSCRIPTION / Complex / TFIID / TAF / TBD-associated Factor
Function / homology
Function and homology information


pre-snoRNP complex / transcription factor TFTC complex / SLIK (SAGA-like) complex / positive regulation of response to cytokine stimulus / C2H2 zinc finger domain binding / box C/D snoRNP assembly / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator ...pre-snoRNP complex / transcription factor TFTC complex / SLIK (SAGA-like) complex / positive regulation of response to cytokine stimulus / C2H2 zinc finger domain binding / box C/D snoRNP assembly / SAGA complex / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / regulation of RNA splicing / negative regulation of cell cycle / aryl hydrocarbon receptor binding / MLL1 complex / RNA polymerase II transcribes snRNA genes / positive regulation of transcription initiation by RNA polymerase II / regulation of DNA repair / RNA polymerase II preinitiation complex assembly / positive regulation of intrinsic apoptotic signaling pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / RNA Polymerase II Pre-transcription Events / response to interleukin-1 / transcription initiation at RNA polymerase II promoter / DNA-templated transcription initiation / mRNA transcription by RNA polymerase II / p53 binding / actin cytoskeleton / HATs acetylate histones / ATPase binding / Regulation of TP53 Activity through Phosphorylation / DNA-binding transcription factor binding / transcription by RNA polymerase II / transcription coactivator activity / transcription cis-regulatory region binding / protein stabilization / positive regulation of apoptotic process / chromatin remodeling / protein heterodimerization activity / negative regulation of cell population proliferation / apoptotic process / DNA damage response / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / regulation of DNA-templated transcription / chromatin / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / identical protein binding / nucleus / cytosol
Similarity search - Function
: / Transcription initiation factor IID, 31kD subunit / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain ...: / Transcription initiation factor IID, 31kD subunit / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / LIS1 homology (LisH) motif profile. / LIS1 homology motif / Histone, subunit A / Histone, subunit A / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Histone-fold / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Transcription initiation factor TFIID subunit 6 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 9
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsHaffke, M. / Berger, I.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Wellcome Trust106115/Z/14/Z United Kingdom
CitationJournal: Nat. Struct. Mol. Biol. / Year: 2018
Title: Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly.
Authors: Antonova, S.V. / Haffke, M. / Corradini, E. / Mikuciunas, M. / Low, T.Y. / Signor, L. / van Es, R.M. / Gupta, K. / Scheer, E. / Vos, H.R. / Tora, L. / Heck, A.J.R. / Timmers, H.T.M. / Berger, I.
History
DepositionNov 28, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 5, 2018Provider: repository / Type: Initial release
Revision 1.1Dec 12, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Dec 19, 2018Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Oct 23, 2024Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transcription initiation factor TFIID subunit 5
B: Transcription initiation factor TFIID subunit 5
C: Transcription initiation factor TFIID subunit 5
D: Transcription initiation factor TFIID subunit 5
E: Transcription initiation factor TFIID subunit 6
F: Transcription initiation factor TFIID subunit 9
G: Transcription initiation factor TFIID subunit 6
H: Transcription initiation factor TFIID subunit 9
I: Transcription initiation factor TFIID subunit 6
J: Transcription initiation factor TFIID subunit 9
K: Transcription initiation factor TFIID subunit 6
L: Transcription initiation factor TFIID subunit 9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)372,90619
Polymers372,65712
Non-polymers2487
Water7,044391
1
A: Transcription initiation factor TFIID subunit 5
E: Transcription initiation factor TFIID subunit 6
F: Transcription initiation factor TFIID subunit 9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,2355
Polymers93,1643
Non-polymers712
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10390 Å2
ΔGint-92 kcal/mol
Surface area34380 Å2
MethodPISA
2
B: Transcription initiation factor TFIID subunit 5
G: Transcription initiation factor TFIID subunit 6
H: Transcription initiation factor TFIID subunit 9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,2004
Polymers93,1643
Non-polymers351
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9920 Å2
ΔGint-81 kcal/mol
Surface area34440 Å2
MethodPISA
3
C: Transcription initiation factor TFIID subunit 5
I: Transcription initiation factor TFIID subunit 6
J: Transcription initiation factor TFIID subunit 9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,2355
Polymers93,1643
Non-polymers712
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9950 Å2
ΔGint-72 kcal/mol
Surface area34300 Å2
MethodPISA
4
D: Transcription initiation factor TFIID subunit 5
K: Transcription initiation factor TFIID subunit 6
L: Transcription initiation factor TFIID subunit 9
hetero molecules


Theoretical massNumber of molelcules
Total (without water)93,2355
Polymers93,1643
Non-polymers712
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9950 Å2
ΔGint-73 kcal/mol
Surface area34310 Å2
MethodPISA
Unit cell
Length a, b, c (Å)339.050, 339.050, 339.050
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number197
Space group name H-MI23
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain E and resid 7 through 98)
21chain G
31(chain I and resid 7 through 98)
41(chain K and resid 7 through 98)
12(chain A and (resid 209 through 747 or resid 756 through 800))
22(chain B and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))
32(chain C and (resid 209 through 747 or resid 756 through 800))
42(chain D and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))
13chain F
23chain H
33chain J
43chain L

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LEULEUALAALA(chain E and resid 7 through 98)EE7 - 983 - 94
211LEULEUALAALAchain GGG7 - 983 - 94
311LEULEUALAALA(chain I and resid 7 through 98)II7 - 983 - 94
411LEULEUALAALA(chain K and resid 7 through 98)KK7 - 983 - 94
112GLYGLYLEULEU(chain A and (resid 209 through 747 or resid 756 through 800))AA209 - 74717 - 555
122THRTHRGLNGLN(chain A and (resid 209 through 747 or resid 756 through 800))AA756 - 800564 - 608
212GLYGLYPROPRO(chain B and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))BB209 - 38017 - 188
222GLNGLNLEULEU(chain B and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))BB417 - 747225 - 555
232THRTHRGLNGLN(chain B and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))BB756 - 800564 - 608
312GLYGLYLEULEU(chain C and (resid 209 through 747 or resid 756 through 800))CC209 - 74717 - 555
322THRTHRGLNGLN(chain C and (resid 209 through 747 or resid 756 through 800))CC756 - 800564 - 608
412GLYGLYPROPRO(chain D and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))DD209 - 38017 - 188
422GLNGLNLEULEU(chain D and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))DD417 - 747225 - 555
432THRTHRGLNGLN(chain D and (resid 209 through 380 or resid 417 through 747 or resid 756 through 800))DD756 - 800564 - 608
113LYSLYSTYRTYRchain FFF5 - 1201 - 116
213LYSLYSTYRTYRchain HHH5 - 1201 - 116
313LYSLYSTYRTYRchain JJJ5 - 1201 - 116
413LYSLYSTYRTYRchain LLL5 - 1201 - 116

NCS ensembles :
ID
1
2
3

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Components

#1: Protein
Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID 100 kDa subunit / TAFII100


Mass: 68950.398 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF5, TAF2D / Production host: unidentified baculovirus / References: UniProt: Q15542
#2: Protein
Transcription initiation factor TFIID subunit 6 / RNA polymerase II TBP-associated factor subunit E / Transcription initiation factor TFIID 70 kDa ...RNA polymerase II TBP-associated factor subunit E / Transcription initiation factor TFIID 70 kDa subunit / TAFII70 / Transcription initiation factor TFIID 80 kDa subunit / TAFII80


Mass: 10888.729 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF6, TAF2E, TAFII70 / Production host: unidentified baculovirus / References: UniProt: P49848
#3: Protein
Transcription initiation factor TFIID subunit 9 / RNA polymerase II TBP-associated factor subunit G / STAF31/32 / Transcription initiation factor ...RNA polymerase II TBP-associated factor subunit G / STAF31/32 / Transcription initiation factor TFIID 31 kDa subunit / TAFII31 / Transcription initiation factor TFIID 32 kDa subunit / TAFII32


Mass: 13325.230 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAF9, TAF2G, TAFII31 / Production host: unidentified baculovirus / References: UniProt: Q16594
#4: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 391 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.42 Å3/Da / Density % sol: 72.2 % / Description: Pyramidal shape
Crystal growTemperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 6.8
Details: 0.1 M Tris/HCl pH6.8, 0.8M Na-Citrate, 0.0-0.15M NaCl

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 1.0044 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 1, 2013
RadiationMonochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0044 Å / Relative weight: 1
ReflectionResolution: 2.5→79.915 Å / Num. obs: 219180 / % possible obs: 99 % / Redundancy: 4.69 % / Biso Wilson estimate: 71.94 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.136 / Rrim(I) all: 0.153 / Χ2: 0.833 / Net I/σ(I): 8.67
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
2.5-2.64.1463.0480.64224360.1883.49191.7
2.6-2.74.7422.2340.99209490.3122.51899.9
2.7-2.84.7941.5631.48180580.4681.75999.9
2.8-2.94.7991.1672.01156860.6031.314100
2.9-34.7970.8082.84136600.7290.91100
3-3.54.7960.2926.41472880.9590.328100
3.5-44.7720.10712.97265940.990.121100
4-54.730.06618.95264260.9940.075100
5-64.6710.06419.81117690.9940.07299.9
6-104.6050.05522.83127440.9960.06299.9
10-304.480.0527.0834410.9950.05799.1
30-504.0740.04924.261080.9960.05691.5
50-1002.8570.05116.93210.9920.06470

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Processing

Software
NameVersionClassification
PHENIX1.12_2829refinement
XSCALEdata scaling
PDB_EXTRACT3.24data extraction
XDSdata reduction
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→79.915 Å / SU ML: 0.45 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 27.39 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.22 10958 5 %
Rwork0.1973 208132 -
obs0.1984 219090 98.99 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 260.34 Å2 / Biso mean: 97.2153 Å2 / Biso min: 46.13 Å2
Refinement stepCycle: final / Resolution: 2.5→79.915 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms24370 0 7 391 24768
Biso mean--79.55 68.85 -
Num. residues----3040
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00324918
X-RAY DIFFRACTIONf_angle_d0.65833699
X-RAY DIFFRACTIONf_chiral_restr0.0443675
X-RAY DIFFRACTIONf_plane_restr0.0034354
X-RAY DIFFRACTIONf_dihedral_angle_d11.59415081
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11E2333X-RAY DIFFRACTION11.468TORSIONAL
12G2333X-RAY DIFFRACTION11.468TORSIONAL
13I2333X-RAY DIFFRACTION11.468TORSIONAL
14K2333X-RAY DIFFRACTION11.468TORSIONAL
21A13741X-RAY DIFFRACTION11.468TORSIONAL
22B13741X-RAY DIFFRACTION11.468TORSIONAL
23C13741X-RAY DIFFRACTION11.468TORSIONAL
24D13741X-RAY DIFFRACTION11.468TORSIONAL
31F2886X-RAY DIFFRACTION11.468TORSIONAL
32H2886X-RAY DIFFRACTION11.468TORSIONAL
33J2886X-RAY DIFFRACTION11.468TORSIONAL
34L2886X-RAY DIFFRACTION11.468TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.5001-2.52850.38463180.3926054637287
2.5285-2.55820.38353340.38376339667391
2.5582-2.58940.42263500.37986637698795
2.5894-2.62220.35173660.362969447310100
2.6222-2.65670.35633630.348669077270100
2.6567-2.69310.36963710.340370467417100
2.6931-2.73160.34413640.339268987262100
2.7316-2.77230.40353690.377370107379100
2.7723-2.81570.39583670.370169767343100
2.8157-2.86180.39353660.345969657331100
2.8618-2.91120.34873670.315169617328100
2.9112-2.96410.27563690.284870217390100
2.9641-3.02110.29263680.277269787346100
3.0211-3.08280.29483670.27569767343100
3.0828-3.14980.28833660.255969627328100
3.1498-3.22310.25863710.244670437414100
3.2231-3.30370.27143660.245969507316100
3.3037-3.3930.26433690.238170207389100
3.393-3.49290.26433670.216669797346100
3.4929-3.60560.233700.197870167386100
3.6056-3.73450.19833670.186169897356100
3.7345-3.8840.21853690.180470047373100
3.884-4.06080.19113690.16670027371100
4.0608-4.27490.15553690.145870167385100
4.2749-4.54270.17023710.140170397410100
4.5427-4.89340.15043710.130370677438100
4.8934-5.38570.16553690.146470037372100
5.3857-6.16480.18543730.162370827455100
6.1648-7.76590.18683730.164770877460100
7.7659-79.95570.17993790.15987161754099
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.1689-0.2991-0.992.6383-0.85592.2719-0.07630.3413-0.0347-0.60930.1401-0.07540.2395-0.0958-0.05210.6547-0.09140.10790.7726-0.0150.7268198.958285.564742.7565
21.5640.0258-0.05780.7025-0.13472.68960.1396-0.19050.63610.07420.0619-0.1185-0.76480.1468-0.18850.6561-0.07480.15570.5798-0.06580.9276184.8952110.455680.0359
31.7921-0.0629-0.37671.27850.35861.93930.15780.01150.3745-0.14470.0963-0.2904-0.35720.3284-0.25820.53-0.08650.17110.5191-0.01370.7533191.6828104.098963.9668
42.22910.3349-0.67263.20450.99281.8782-0.1621-0.5988-0.05340.72150.07060.170.2705-0.05350.14050.88490.17830.11061.11430.0790.7704139.557986.4203130.8732
51.7401-0.18860.53720.8062-0.10192.8411-0.0580.00270.53920.05770.1077-0.0503-0.7732-0.0258-0.0730.64970.06370.0940.5667-0.01640.7912157.7766111.582795.8337
61.5309-0.23430.12741.3768-0.21122.0475-0.0692-0.27840.23860.25590.16180.1992-0.382-0.3532-0.08750.67770.13710.12420.7304-0.04090.7356149.9089105.0799111.2378
71.8227-0.54750.87011.6071-0.26811.67040.01060.29310.1919-0.5225-0.05730.1949-0.2233-0.05530.09850.65090.0225-0.11130.73140.09470.6227143.846681.766840.0535
81.34660.0675-0.22611.16830.03941.8585-0.02170.0347-0.1786-0.02860.00440.09310.2877-0.10920.02650.49270.0034-0.0620.51660.02630.6122152.678559.376167.8932
91.85850.1283-0.05691.6669-0.1171.5011-0.0936-1.10140.24580.5510.1582-0.3676-0.00480.4265-0.06410.84940.2087-0.18081.5498-0.27240.7782195.059376.5338133.6545
101.4812-0.2687-0.3191.18140.12421.5387-0.1115-0.3843-0.12460.25060.1142-0.07570.28760.25980.00240.52260.129-0.06110.6970.05970.5659184.475856.4885104.3843
116.47163.8069-2.59483.85520.33863.2796-0.31650.35290.0271-1.1173-0.7848-1.8359-0.04461.29520.96790.77170.0680.1471.32120.20631.1406210.369874.385258.8918
122.0399-1.53291.68916.0843-1.04831.8254-0.0596-0.1652-1.0322-0.63470.0776-1.52421.2241.18030.12461.01160.16740.08560.88460.04731.0617203.454353.449369.6647
130.9887-1.0772-0.33372.04651.69482.4977-0.17240.01160.2258-0.75730.4694-0.24830.06440.3756-0.18180.6822-0.02980.09370.79370.05540.8601198.993372.893966.1201
143.03670.833-0.43158.09530.29312.75250.1925-0.56140.14680.1626-0.2191-0.5612-0.2280.0487-0.10990.57820.0260.03150.77080.03240.7835196.866483.52576.123
154.36734.8781-4.73255.6471-5.39845.17610.1613-0.7124-0.24940.39310.3461-0.0276-0.32050.5184-0.42460.65090.0816-0.11441.0945-0.04460.8229186.688368.624974.6593
168.9821-3.32645.28573.7134-3.38624.0665-0.2183-0.1155-0.09120.15680.1279-0.32420.25180.6130.33180.50710.23090.01410.8907-0.0560.6805197.662251.442590.5238
171.9449-2.22181.73893.53140.19876.6165-0.4352-0.2022-1.1669-0.4101-0.734-0.02440.49311.94080.97090.88450.21020.06021.39220.14611.0952206.241260.949482.0861
185.92271.3385-0.28517.613-0.16212.11090.17850.17890.693-0.51430.1899-1.8888-0.27731.40190.17270.6661-0.09370.02881.07640.12791.2558209.214677.962470.3093
195.2732.61761.56352.29781.27495.34870.0984-0.41040.0180.5352-0.3144-0.40720.68550.15930.24510.61640.06950.09930.69170.0690.8301196.104463.250673.7977
206.23080.4671-0.61377.8295-0.37119.91630.02690.8345-0.4147-1.0806-0.08370.88031.1303-0.21750.33210.9711-0.0509-0.04430.90170.00290.7461189.579755.990463.7092
214.53110.53134.159.7079-4.52516.39050.71190.067-1.0125-0.67820.34010.57440.48510.6197-0.70360.43490.06750.04280.8144-0.08420.9948185.210476.440364.4576
224.22260.20624.65613.4419-2.8657.89750.54110.5059-0.815-0.3711-0.37820.39910.67190.6573-0.45840.62580.07840.05330.74720.03810.8624174.172787.784456.6024
233.0538-2.3776-1.7633.05023.66468.89760.3342-0.0028-0.08070.6332-0.67380.2537-0.0663-1.11960.41980.6827-0.04020.18470.84610.10860.8789163.2656104.201765.5778
243.86441.13950.19287.36274.30345.3720.20790.1795-0.62970.487-0.80220.49990.7393-0.31710.20420.70370.03110.07460.8907-0.05030.9606162.473998.235578.8764
253.1089-1.2456-0.9126.92425.52394.3787-0.26550.0334-0.28950.8654-1.01681.4571-0.0938-1.22461.0360.91220.14430.06221.22210.05791.1207128.757575.8757112.8629
266.3029-2.0039-1.51478.10243.65352.3361-0.13170.5826-1.40470.5421-0.45851.49530.964-1.16050.9240.9411-0.11440.15261.00240.0141.2034133.250856.1857102.4093
271.4831.1721-0.86292.3257-2.54392.9688-0.1589-0.0691-0.10410.5920.17830.23970.1146-0.11-0.08380.70170.0910.13270.96240.05670.8814139.595274.8293107.1591
284.9196-3.5462-0.71829.64491.7262.24880.08920.4221-0.0974-0.2469-0.30180.55030.01310.20010.17730.5438-0.00610.05460.77030.01620.7373142.936285.838297.9436
296.4351-3.7342-3.40432.17362.02158.46250.7112-0.0251-0.39960.07270.3248-0.75730.2841-0.0406-0.61580.748-0.02990.00851.09550.12240.9209152.132469.918198.3083
304.5262.48054.71712.91973.36385.436-0.28310.1568-0.3639-0.04470.29070.17030.3348-0.43670.33720.5512-0.17980.10580.82980.04850.8177139.062754.904281.5436
315.88451.3917-2.19094.4268-5.44046.7232-0.9610.8333-0.9422-0.5790.22710.41111.0158-1.44690.35510.6409-0.11560.02951.0918-0.10451.0868132.63363.321288.0527
324.24930.5596-1.66414.38880.57626.81360.10790.20470.340.57660.42271.3409-0.2154-0.8409-0.09720.77360.16220.05211.05540.031.1031129.586679.255102.1313
337.7008-4.96133.12783.4134-2.44556.3067-0.1472-0.171-0.0927-0.41170.07110.01280.7616-0.01090.17850.69220.04240.10330.81630.03840.8195141.762165.448898.8513
346.67993.9389-0.87998.1539-1.12896.9682-0.0371-0.5119-0.79810.4080.0193-0.52250.59120.15580.08090.95810.08680.08240.90090.150.8326147.308657.1133108.5323
356.04436.44633.50648.11993.20483.10890.2761-0.4392-0.42090.63440.1275-0.07810.1935-0.5498-0.20690.64720.11840.04240.89410.03520.8352161.627983.3787115.1441
362.80442.4357-1.82122.7839-2.58557.11750.0824-0.1631-0.2178-0.2865-0.0575-0.5121-0.31740.3659-0.00270.76770.04560.06820.8437-0.11660.8284178.273102.1399109.8255
372.13352.04810.49455.8037-5.48749.45830.3773-0.5-0.2249-0.002-1.0452-0.88570.97980.95940.63170.66730.0489-0.00551.02550.09590.9351178.584396.95696.2219
382.3014-1.4472-0.36022.64181.6145.2017-0.1726-0.31950.6832-0.5868-0.60121.4898-0.8684-0.97540.80440.93080.2375-0.09210.87910.07821.0786135.0236104.126463.0522
391.78641.2105-0.39359.1588-1.03713.63510.06430.18870.1013-0.86250.1720.644-0.3527-0.325-0.1370.60010.0978-0.06510.69990.03340.6806142.611393.687763.8245
407.32321.79191.51938.78221.22143.62760.3899-0.65680.15560.1581-0.39630.32040.01540.1025-0.03970.55980.1353-0.03820.60940.03420.6546143.968682.510373.351
410.46430.40140.37493.29691.540.7553-0.2492-0.0682-0.269-0.25220.0597-0.23020.4053-0.28730.1390.68590.08520.05850.91730.21330.8733153.7558102.691276.0738
423.4433-2.96920.34626.64172.89962.7744-0.3331-0.46410.64150.21510.43160.6355-0.7112-1.33540.24150.76730.4020.03871.04940.00761.0188136.4759116.533494.6979
431.3735-0.93050.80121.1563-2.0225.131-0.5647-0.33780.7788-0.2211-0.28630.3853-1.7623-1.23680.58941.06660.204-0.05581.2115-0.0271.2611133.9485104.825479.5984
443.3853-0.37711.16513.91081.46999.13390.02130.2045-0.5734-0.6177-0.01051.182-0.0127-0.92010.13570.6680.075-0.07810.985-0.04161.1243132.08388.379366.9581
459.25155.4149-3.26783.3588-2.69995.6920.3580.07010.16830.9146-0.26740.1099-1.06690.0258-0.05890.83490.1234-0.06930.6496-0.02220.8114144.9011102.902972.3458
462.6881-0.19330.87111.90921.91385.65170.24170.26050.865-0.45880.088-1.2701-1.24230.3898-0.07661.11740.15670.1180.78730.16921.0071151.9228110.82563.4125
475.6112-6.0196-3.75986.514.02292.50390.3451-0.20610.08760.049-0.0840.1297-0.4294-0.43520.0760.57640.0342-0.03690.89320.08160.7386156.376790.237963.0269
486.1287-5.9314-3.92535.79873.47025.3180.45260.39110.5745-0.6643-0.039-0.8909-0.3364-0.0887-0.06040.6569-0.05650.00160.70210.07420.7845167.971579.277455.576
492.6995-0.52130.55011.7103-1.5366.3057-0.182-0.0456-0.03880.047-0.4968-0.43370.240.73590.57180.53040.0364-0.02760.70870.05670.6521177.348961.888568.6318
504.30536.2444.90029.96185.94287.64620.0447-1.06840.81161.6022-1.5111-0.1362-1.18250.65230.74850.9045-0.10660.03051.06770.22121.131180.199773.589279.5779
511.7434-1.31850.3565.6142-3.24874.471-0.28760.01580.27670.4968-0.1907-0.7718-0.75170.61460.50370.931-0.1553-0.19491.3245-0.28331.3056206.210299.99111.4864
520.1883-0.6187-0.74777.2611.47722.6904-0.1561-0.35590.1630.30330.4227-0.7554-0.1590.3604-0.25130.5901-0.0093-0.11081.0156-0.14510.9759197.058685.34106.7068
535.8297-5.63040.51935.84250.50032.5710.79120.18220.1748-0.0194-0.16580.12450.26740.6091-0.13280.8411-0.04350.01841.4291-0.23480.9998186.871995.0636100.9547
542.34541.42151.17343.0945-1.16042.13750.1788-0.41480.36950.017-0.2297-0.4703-1.00191.1669-0.14430.7088-0.1760.03440.9302-0.30461.1507201.1305110.455885.8722
553.91360.7929-0.85412.99463.27784.3826-0.6611-0.07260.4024-0.04250.07040.0814-0.89991.27560.53981.0527-0.1034-0.01921.2822-0.06581.3307206.6726101.907192.5945
563.24-0.0586-0.03861.4003-0.4663.22270.0449-0.58050.06730.64970.1202-1.098-0.55021.3596-0.01070.8954-0.0386-0.09941.3578-0.14471.3632209.176488.4151107.8447
573.1977-2.0671-0.83488.47151.33433.3708-0.1388-0.42930.1861-0.13680.223-0.5566-0.69060.2421-0.02560.8967-0.0931-0.15921.0309-0.1441.0355197.465898.7206104.1961
585.90315.95230.80646.0081.03255.08380.2489-0.70510.48970.81090.1510.2053-0.30130.0922-0.42211.0212-0.05-0.08931.0117-0.25041.0779189.235102.847112.4016
599.14552.267-3.39232.7332-2.63762.98560.5492-0.42260.63021.08160.29280.0765-0.59320.5471-0.26310.68520.0879-0.02570.9746-0.2190.8216175.194480.5376115.6464
605.663.30853.68965.29023.97063.3912-0.0418-0.4090.2045-0.3202-0.45760.1945-0.1259-1.10870.53980.70960.17650.00220.89230.0260.6787159.25962.3178107.6275
615.7048-1.0769-0.28143.36854.27615.60890.1759-0.02481.2251-0.7675-0.51380.395-0.8648-0.59160.20570.82250.12730.00610.8763-0.05440.8226160.417968.240394.4005
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 207 through 373 )A207 - 373
2X-RAY DIFFRACTION2chain 'A' and (resid 374 through 581 )A374 - 581
3X-RAY DIFFRACTION3chain 'A' and (resid 582 through 800 )A582 - 800
4X-RAY DIFFRACTION4chain 'B' and (resid 209 through 373 )B209 - 373
5X-RAY DIFFRACTION5chain 'B' and (resid 374 through 581 )B374 - 581
6X-RAY DIFFRACTION6chain 'B' and (resid 582 through 800 )B582 - 800
7X-RAY DIFFRACTION7chain 'C' and (resid 209 through 373 )C209 - 373
8X-RAY DIFFRACTION8chain 'C' and (resid 374 through 800 )C374 - 800
9X-RAY DIFFRACTION9chain 'D' and (resid 209 through 373 )D209 - 373
10X-RAY DIFFRACTION10chain 'D' and (resid 374 through 800 )D374 - 800
11X-RAY DIFFRACTION11chain 'E' and (resid 6 through 15 )E6 - 15
12X-RAY DIFFRACTION12chain 'E' and (resid 16 through 32 )E16 - 32
13X-RAY DIFFRACTION13chain 'E' and (resid 33 through 60 )E33 - 60
14X-RAY DIFFRACTION14chain 'E' and (resid 61 through 78 )E61 - 78
15X-RAY DIFFRACTION15chain 'E' and (resid 79 through 83 )E79 - 83
16X-RAY DIFFRACTION16chain 'E' and (resid 84 through 98 )E84 - 98
17X-RAY DIFFRACTION17chain 'F' and (resid 5 through 15 )F5 - 15
18X-RAY DIFFRACTION18chain 'F' and (resid 16 through 32 )F16 - 32
19X-RAY DIFFRACTION19chain 'F' and (resid 33 through 60 )F33 - 60
20X-RAY DIFFRACTION20chain 'F' and (resid 61 through 81 )F61 - 81
21X-RAY DIFFRACTION21chain 'F' and (resid 82 through 88 )F82 - 88
22X-RAY DIFFRACTION22chain 'F' and (resid 89 through 100 )F89 - 100
23X-RAY DIFFRACTION23chain 'F' and (resid 101 through 110 )F101 - 110
24X-RAY DIFFRACTION24chain 'F' and (resid 111 through 120 )F111 - 120
25X-RAY DIFFRACTION25chain 'G' and (resid 7 through 15 )G7 - 15
26X-RAY DIFFRACTION26chain 'G' and (resid 16 through 32 )G16 - 32
27X-RAY DIFFRACTION27chain 'G' and (resid 33 through 60 )G33 - 60
28X-RAY DIFFRACTION28chain 'G' and (resid 61 through 78 )G61 - 78
29X-RAY DIFFRACTION29chain 'G' and (resid 79 through 83 )G79 - 83
30X-RAY DIFFRACTION30chain 'G' and (resid 84 through 98 )G84 - 98
31X-RAY DIFFRACTION31chain 'H' and (resid 5 through 12 )H5 - 12
32X-RAY DIFFRACTION32chain 'H' and (resid 13 through 32 )H13 - 32
33X-RAY DIFFRACTION33chain 'H' and (resid 33 through 60 )H33 - 60
34X-RAY DIFFRACTION34chain 'H' and (resid 61 through 81 )H61 - 81
35X-RAY DIFFRACTION35chain 'H' and (resid 82 through 100 )H82 - 100
36X-RAY DIFFRACTION36chain 'H' and (resid 101 through 110 )H101 - 110
37X-RAY DIFFRACTION37chain 'H' and (resid 111 through 120 )H111 - 120
38X-RAY DIFFRACTION38chain 'I' and (resid 5 through 32 )I5 - 32
39X-RAY DIFFRACTION39chain 'I' and (resid 33 through 60 )I33 - 60
40X-RAY DIFFRACTION40chain 'I' and (resid 61 through 78 )I61 - 78
41X-RAY DIFFRACTION41chain 'I' and (resid 79 through 88 )I79 - 88
42X-RAY DIFFRACTION42chain 'I' and (resid 89 through 98 )I89 - 98
43X-RAY DIFFRACTION43chain 'J' and (resid 5 through 15 )J5 - 15
44X-RAY DIFFRACTION44chain 'J' and (resid 16 through 32 )J16 - 32
45X-RAY DIFFRACTION45chain 'J' and (resid 33 through 60 )J33 - 60
46X-RAY DIFFRACTION46chain 'J' and (resid 61 through 81 )J61 - 81
47X-RAY DIFFRACTION47chain 'J' and (resid 82 through 88 )J82 - 88
48X-RAY DIFFRACTION48chain 'J' and (resid 89 through 100 )J89 - 100
49X-RAY DIFFRACTION49chain 'J' and (resid 101 through 115 )J101 - 115
50X-RAY DIFFRACTION50chain 'J' and (resid 116 through 120 )J116 - 120
51X-RAY DIFFRACTION51chain 'K' and (resid 6 through 32 )K6 - 32
52X-RAY DIFFRACTION52chain 'K' and (resid 33 through 78 )K33 - 78
53X-RAY DIFFRACTION53chain 'K' and (resid 79 through 83 )K79 - 83
54X-RAY DIFFRACTION54chain 'K' and (resid 84 through 98 )K84 - 98
55X-RAY DIFFRACTION55chain 'L' and (resid 5 through 12 )L5 - 12
56X-RAY DIFFRACTION56chain 'L' and (resid 13 through 26 )L13 - 26
57X-RAY DIFFRACTION57chain 'L' and (resid 27 through 67 )L27 - 67
58X-RAY DIFFRACTION58chain 'L' and (resid 68 through 81 )L68 - 81
59X-RAY DIFFRACTION59chain 'L' and (resid 82 through 100 )L82 - 100
60X-RAY DIFFRACTION60chain 'L' and (resid 101 through 110 )L101 - 110
61X-RAY DIFFRACTION61chain 'L' and (resid 111 through 120 )L111 - 120

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