SASDC83: Dimeric apoptosis regulator BAX (Bcl-2 associated X)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDC83

試料

Dimeric apoptosis regulator BAX (Bcl-2 associated X)

• Apoptosis regulator BAX (Bcl-2 associated X) (protein), BAX, Homo sapiens

機能・相同性

分子機能:

T cell homeostatic proliferation / release of matrix enzymes from mitochondria / positive regulation of developmental pigmentation / BAX complex / protein insertion into mitochondrial membrane / B cell receptor apoptotic signaling pathway / positive regulation of reproductive process / positive regulation of motor neuron apoptotic process / regulation of mammary gland epithelial cell proliferation / Activation, translocation and oligomerization of BAX / spermatid differentiation / NTRK3 as a dependence receptor / Sertoli cell proliferation / positive regulation of apoptotic DNA fragmentation / positive regulation of B cell apoptotic process / development of secondary sexual characteristics / glycosphingolipid metabolic process / positive regulation of mitochondrial membrane permeability involved in apoptotic process / retinal cell programmed cell death / B cell homeostatic proliferation / B cell negative selection / BAK complex / regulation of mitochondrial membrane permeability involved in programmed necrotic cell death / negative regulation of endoplasmic reticulum calcium ion concentration / apoptotic process involved in embryonic digit morphogenesis / mitochondrial permeability transition pore complex / Release of apoptotic factors from the mitochondria / mitochondrial fragmentation involved in apoptotic process / apoptotic process involved in blood vessel morphogenesis / post-embryonic camera-type eye morphogenesis / Transcriptional regulation by RUNX2 / establishment or maintenance of transmembrane electrochemical gradient / apoptotic process involved in mammary gland involution / positive regulation of apoptotic process involved in mammary gland involution / regulation of nitrogen utilization / B cell apoptotic process / endoplasmic reticulum calcium ion homeostasis / positive regulation of epithelial cell apoptotic process / fertilization / calcium ion transport into cytosol / mitochondrial fusion / epithelial cell apoptotic process / myeloid cell homeostasis / Bcl-2 family protein complex / motor neuron apoptotic process / execution phase of apoptosis / thymocyte apoptotic process / pore complex / hypothalamus development / positive regulation of IRE1-mediated unfolded protein response / odontogenesis of dentin-containing tooth / positive regulation of release of cytochrome c from mitochondria / apoptotic mitochondrial changes / germ cell development / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / positive regulation of calcium ion transport into cytosol / BH3 domain binding / vagina development / negative regulation of mitochondrial membrane potential / B cell homeostasis / intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of apoptotic signaling pathway / extrinsic apoptotic signaling pathway via death domain receptors / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / blood vessel remodeling / cellular response to unfolded protein / Pyroptosis / ectopic germ cell programmed cell death / response to axon injury / negative regulation of fibroblast proliferation / negative regulation of protein binding / ovarian follicle development / positive regulation of intrinsic apoptotic signaling pathway / supramolecular fiber organization / extrinsic apoptotic signaling pathway / response to salt stress / release of sequestered calcium ion into cytosol / Hsp70 protein binding / extrinsic apoptotic signaling pathway in absence of ligand / regulation of mitochondrial membrane potential / homeostasis of number of cells within a tissue / intrinsic apoptotic signaling pathway / positive regulation of release of sequestered calcium ion into cytosol / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / release of cytochrome c from mitochondria / response to gamma radiation / positive regulation of protein-containing complex assembly / apoptotic signaling pathway / kidney development / cerebral cortex development / cellular response to virus / response to toxic substance / neuron migration / intrinsic apoptotic signaling pathway in response to DNA damage / cellular response to UV / nuclear envelope / positive regulation of neuron apoptotic process / retina development in camera-type eye / channel activity / regulation of apoptotic process

ドメイン・相同性:

Apoptosis regulator, Bcl-2, BH3 motif, conserved site / Apoptosis regulator, Bcl-2 family BH3 motif signature. / Apoptosis regulator, Bcl-2, BH1 motif, conserved site / Apoptosis regulator, Bcl-2 family BH1 motif signature. / Apoptosis regulator, Bcl-2, BH2 motif, conserved site / Apoptosis regulator, Bcl-2 family BH2 motif signature. / Bcl-2 family / BCL (B-Cell lymphoma); contains BH1, BH2 regions / Bcl2-like / Bcl-2, Bcl-2 homology region 1-3 / Apoptosis regulator proteins, Bcl-2 family / BCL2-like apoptosis inhibitors family profile. / Bcl-2-like superfamily

構成要素:

Apoptosis regulator BAX

• 生物種: Homo sapiens (ヒト)
• 引用: 年: 2017; タイトル: Oligomerization process of Bcl-2 associated X protein revealed from intermediate structures in solution; 著者: Shih O / Yeh Y / Liao K / Sung T / Chiang Y

登録者

• Orion Shih (National Synchrotron Radiation Research Center)

モデル

• モデル #1239: ; タイプ: mix / ソフトウェア: CORAL / ダミー原子の半径: 1.90 A / カイ2乗値: 1.25; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Dimeric apoptosis regulator BAX (Bcl-2 associated X); 試料濃度: 2 mg/ml
• バッファ: 名称: 20mM sodium phosphate 100mM NaCl / pH: 8 / コメント: 20mM sodium phosphate, 100mM NaCl

要素 #668

名称: BAX / タイプ: protein / 記述: Apoptosis regulator BAX (Bcl-2 associated X) / 分子量: 21.184 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q07812
配列:

MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG

実験情報

• ビーム: 設備名称: Taiwan Photon Source 23A / 地域: NSRRC, Hsinchu / 国: Taiwan / 線源: X-ray synchrotron / 波長: 0.08266 Å / スペクトロメータ・検出器間距離: 3.17 mm
• 検出器: 名称: Pilatus 1M-F / Pixsize x: 172 mm

スキャン

タイトル: Dimeric apoptosis regulator BAX (Bcl-2 associated X)
測定日: 2015年5月28日 / セル温度: 15 °C / 照射時間: 30 sec. / フレーム数: 3 / 単位: 1/A /

	Min	Max
Q	0.0144	0.2991

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 199 /

	Min	Max
Q	0.0185	0.27071
P(R) point	1	199
R	0	95.24

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	39.5 kDa	-
体積	-	85.57 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.01585	0.000135	0.01578	0.0002
慣性半径, Rg	3.01 nm	0.03	2.97 nm	0.06

	Min	Max
D	-	9.52
Guinier point	6	38