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- SASDBK9: Geobacillus stearothermophilus DnaB full-length (GstDnaBFL) -

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Basic information

Entry
Database: SASBDB / ID: SASDBK9
SampleGeobacillus stearothermophilus DnaB full-length
  • Geobacillus stearothermophilus DnaB full-length (protein), GstDnaBFL, Geobacillus stearothermophilus
Function / homologyDnaD domain / DnaD-like domain superfamily / Replication initiation and membrane attachment / Replication initiation and membrane attachment protein
Function and homology information
Biological speciesGeobacillus stearothermophilus (bacteria)
CitationDate: 2017 Sep 22
Title: Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein
Authors: Li Y / Naveen V / Lin M
Contact author
  • YiChing Li (Academia Sinica, Taipei, Taiwan)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1101
Type: dummy / Radius of dummy atoms: 1.90 A / Chi-square value: 1.43
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Geobacillus stearothermophilus DnaB full-length / Specimen concentration: 2.00-10.00
BufferName: 20 mM Tris, 300 mM NaCl and 5 mM β-ME / pH: 8
Entity #568Name: GstDnaBFL / Type: protein
Description: Geobacillus stearothermophilus DnaB full-length
Formula weight: 53.494 / Num. of mol.: 4 / Source: Geobacillus stearothermophilus / References: UniProt: A0A0K2H6I1
Sequence: MQHHWKELIA VDRYTVQSRG VLQEVDRKVL TLLYQPLIGC RALALYMTLW GELELLDGQE ATHHRLMALM QCGLPDIYSE RLKLEGIGLL DTYVHAKEAD EPKLFLYELR PPLAPDQFFR DEMLSVFLRR QVGRHLFIQL SNFFARPSID ETKFTQVTRS FSDVFSAVPA ...Sequence:
MQHHWKELIA VDRYTVQSRG VLQEVDRKVL TLLYQPLIGC RALALYMTLW GELELLDGQE ATHHRLMALM QCGLPDIYSE RLKLEGIGLL DTYVHAKEAD EPKLFLYELR PPLAPDQFFR DEMLSVFLRR QVGRHLFIQL SNFFARPSID ETKFTQVTRS FSDVFSAVPA EQIVAGLSEE AGPELLEGKD HIRRDEASYV LDDGVFDFEL FFAGLSKQLV PRRAVTAKVK EAIKKLAFLY GIPPLEMQKL VLGVIDPAYH IDIDALRRAA REWYELEHGG VEPRLVERVQ PLAYRTMENI EPRTKEEQLM KQLETISPRQ LLKEISGGAE PSLADLQLIE DVMFKQQLLP GVVNVLIYYV MLRTNMKLSK KYVEKIASHW ARKKVKTVKE AMELAKEEAK TYQNWANEKE KGTRTVRKVV RTEIVPDWLN MDYSQPDDDD FDVEQARKEL EERLKKYRDE S

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Experimental information

BeamInstrument name: Taiwan Photon Source 23A / City: NSRRC, Hsinchu / : Taiwan / Type of source: X-ray synchrotron / Wavelength: 0.08285 Å
DetectorName: Flat Panel sensor C10158DK-3957(X) / Pixsize x: 50 mm
Scan
Title: Geobacillus stearothermophilus DnaB full-length / Measurement date: Mar 12, 2015 / Cell temperature: 15 °C / Exposure time: 30 sec. / Number of frames: 10 / Unit: 1/A /
MinMax
Q0.0112 0.3317
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 228 /
MinMax
Q0.01123 0.3317
P(R) point1 228
R0 210
Result
D max: 20 / Type of curve: merged
ExperimentalStandardPorod
MW53.526 kDa199.135 kDa-
Volume--431.66 nm3

P(R)GuinierP(R) error
Forward scattering, I00.191 0.191 -
Radius of gyration, Rg5.745 nm5.745 nm0.199

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