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- SASDBJ9: Geobacillus stearothermophilus DnaB1-300 (GstDnaB1-300) -

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Basic information

Entry
Database: SASBDB / ID: SASDBJ9
SampleGeobacillus stearothermophilus DnaB1-300
  • Geobacillus stearothermophilus DnaB1-300 (protein), GstDnaB1-300, Geobacillus stearothermophilus
Function / homologyDnaD domain / DnaD-like domain superfamily / Replication initiation and membrane attachment / Replication initiation and membrane attachment protein
Function and homology information
Biological speciesGeobacillus stearothermophilus (bacteria)
CitationDate: 2017 Sep 22
Title: Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein
Authors: Li Y / Naveen V / Lin M
Contact author
  • YiChing Li (Academia Sinica, Taipei, Taiwan)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1100
Type: dummy / Radius of dummy atoms: 1.90 A / Chi-square value: 1.30
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Geobacillus stearothermophilus DnaB1-300 / Specimen concentration: 10.00-18.00
BufferName: 20 mM Tris, 300 mM NaCl and 5 mM β-ME / pH: 8
Entity #566Name: GstDnaB1-300 / Type: protein / Description: Geobacillus stearothermophilus DnaB1-300 / Formula weight: 34.508 / Num. of mol.: 4 / Source: Geobacillus stearothermophilus / References: UniProt: A0A0K2H6I1
Sequence: MQHHWKELIA VDRYTVQSRG VLQEVDRKVL TLLYQPLIGC RALALYMTLW GELELLDGQE ATHHRLMALM QCGLPDIYSE RLKLEGIGLL DTYVHAKEAD EPKLFLYELR PPLAPDQFFR DEMLSVFLRR QVGRHLFIQL SNFFARPSID ETKFTQVTRS FSDVFSAVPA ...Sequence:
MQHHWKELIA VDRYTVQSRG VLQEVDRKVL TLLYQPLIGC RALALYMTLW GELELLDGQE ATHHRLMALM QCGLPDIYSE RLKLEGIGLL DTYVHAKEAD EPKLFLYELR PPLAPDQFFR DEMLSVFLRR QVGRHLFIQL SNFFARPSID ETKFTQVTRS FSDVFSAVPA EQIVAGLSEE AGPELLEGKD HIRRDEASYV LDDGVFDFEL FFAGLSKQLV PRRAVTAKVK EAIKKLAFLY GIPPLEMQKL VLGVIDPAYH IDIDALRRAA REWYELEHGG VEPRLVERVQ PLAYRTMENI

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Experimental information

BeamInstrument name: Taiwan Photon Source 23A / City: NSRRC, Hsinchu / 国: Taiwan / Type of source: X-ray synchrotron / Wavelength: 0.08285 Å
DetectorName: Flat Panel sensor C10158DK-3957(X) / Pixsize x: 50 mm
Scan
Title: Geobacillus stearothermophilus DnaB1-300 / Measurement date: Oct 18, 2015 / Cell temperature: 15 °C / Exposure time: 30 sec. / Number of frames: 13 / Unit: 1/A /
MinMax
Q0.0075 0.3526
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 244 /
MinMax
Q0.00751 0.3526
P(R) point1 244
R0 110
Result
D max: 11 / Type of curve: merged
ExperimentalStandardPorod
MW34.547 kDa128.78 kDa-
Volume--314.75 nm3

P(R)Guinier
Forward scattering, I00.868 0.868
Radius of gyration, Rg3.544 nm3.542 nm

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