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- PDB-6mzg: Structural Basis of Tubulin Recruitment and Assembly by Microtubu... -

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Basic information

Entry
Database: PDB / ID: 6mzg
TitleStructural Basis of Tubulin Recruitment and Assembly by Microtubule Polymerases with Tumor Overexpressed Gene (TOG) Domain Arrays
Components
  • Protein Stu2p/Alp14p
  • Truncated Darpin-D1
  • Tubulin alpha-1A chain
  • Tubulin beta chain
KeywordsCELL CYCLE / Microtubule / Tubulin / Tubulin polymerization / Heat repeats / Microtubule polymerase / Protofilament / TOG arrays / and Unfurled assembly.
Function / homology
Function and homology information


supramolecular complex / microtubule plus end polymerase / establishment or maintenance of microtubule cytoskeleton polarity / microtubule plus-end binding / motile cilium / spindle organization / microtubule polymerization / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration ...supramolecular complex / microtubule plus end polymerase / establishment or maintenance of microtubule cytoskeleton polarity / microtubule plus-end binding / motile cilium / spindle organization / microtubule polymerization / structural constituent of cytoskeleton / microtubule cytoskeleton organization / neuron migration / mitotic cell cycle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / hydrolase activity / GTPase activity / GTP binding / metal ion binding / cytoplasm
Similarity search - Function
XMAP215 family / : / XMAP215/Dis1/CLASP, TOG domain / TOG domain / TOG / Helix hairpin bin / Ankyrin repeat-containing domain / Tubulin/FtsZ, C-terminal domain / Tubulin/FtsZ, GTPase domain / Leucine-rich Repeat Variant ...XMAP215 family / : / XMAP215/Dis1/CLASP, TOG domain / TOG domain / TOG / Helix hairpin bin / Ankyrin repeat-containing domain / Tubulin/FtsZ, C-terminal domain / Tubulin/FtsZ, GTPase domain / Leucine-rich Repeat Variant / Leucine-rich Repeat Variant / 60s Ribosomal Protein L30; Chain: A; / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Armadillo-like helical / Helix Hairpins / Alpha Horseshoe / Armadillo-type fold / Rossmann fold / 2-Layer Sandwich / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Protein Stu2p/Alp14p / Tubulin alpha-1A chain / Tubulin beta chain
Similarity search - Component
Biological speciesLachancea kluyveri NRRL Y-12651 (fungus)
Escherichia coli (E. coli)
Sus scrofa (pig)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.208 Å
AuthorsNithianantham, S. / Al-Bassam, J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM110283 United States
CitationJournal: Elife / Year: 2018
Title: Structural basis of tubulin recruitment and assembly by microtubule polymerases with Tumor Overexpressed Gene (TOG) domain arrays.
Authors: Nithianantham, S. / Cook, B.D. / Beans, M. / Guo, F. / Chang, F. / Al-Bassam, J.
History
DepositionNov 5, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 28, 2018Provider: repository / Type: Initial release
Revision 1.1Jan 1, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Mar 13, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1A chain
B: Tubulin beta chain
C: Tubulin alpha-1A chain
D: Tubulin beta chain
E: Protein Stu2p/Alp14p
F: Truncated Darpin-D1
G: Tubulin alpha-1A chain
H: Tubulin beta chain
I: Tubulin alpha-1A chain
J: Tubulin beta chain
K: Protein Stu2p/Alp14p
L: Truncated Darpin-D1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)560,37328
Polymers556,31312
Non-polymers4,06016
Water00
1
A: Tubulin alpha-1A chain
B: Tubulin beta chain
C: Tubulin alpha-1A chain
D: Tubulin beta chain
E: Protein Stu2p/Alp14p
F: Truncated Darpin-D1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)280,18614
Polymers278,1566
Non-polymers2,0308
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: Tubulin alpha-1A chain
H: Tubulin beta chain
I: Tubulin alpha-1A chain
J: Tubulin beta chain
K: Protein Stu2p/Alp14p
L: Truncated Darpin-D1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)280,18614
Polymers278,1566
Non-polymers2,0308
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)115.130, 194.990, 149.570
Angle α, β, γ (deg.)90.000, 90.190, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 4 types, 12 molecules ACGIBDHJEKFL

#1: Protein
Tubulin alpha-1A chain / Alpha-tubulin 1 / Tubulin alpha-1 chain


Mass: 50121.266 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: Brain / References: UniProt: P02550
#2: Protein
Tubulin beta chain / Beta-tubulin


Mass: 49907.770 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / Organ: Brain / References: UniProt: P02554
#3: Protein Protein Stu2p/Alp14p


Mass: 63099.242 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Protein was obtained from cDNA (Lachancea kluyveri NRRL Y-12651 chromosome)
Source: (gene. exp.) Lachancea kluyveri NRRL Y-12651 (fungus)
Gene: SKLU-Cont10078 / Plasmid: pLIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: A0A493R6X8*PLUS
#4: Protein Truncated Darpin-D1


Mass: 14998.981 Da / Num. of mol.: 2 / Mutation: N-terminal truncation (residues 1-40)
Source method: isolated from a genetically manipulated source
Details: N-terminal residues (1-40) of Darpin-D1 were truncated.
Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: pLIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21

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Non-polymers , 3 types, 16 molecules

#5: Chemical
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Mg
#7: Chemical
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.02 Å3/Da / Density % sol: 59.24 % / Description: Rectangular crystal
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 50 mM PIPES, 100 mM MgCl2 [pH 7.0], and 10-15% PEG-8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 25, 2014
RadiationMonochromator: Cryo-Cooled double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
Reflection twinOperator: h,-k,-l / Fraction: 0.49
ReflectionResolution: 3.2→149.571 Å / Num. obs: 104265 / % possible obs: 96.2 % / Redundancy: 2.9 % / Biso Wilson estimate: 46.6 Å2 / Rpim(I) all: 0.105 / Rrim(I) all: 0.186 / Rsym value: 0.133 / Net I/av σ(I): 5.2 / Net I/σ(I): 5.8
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsRpim(I) allRrim(I) allRsym value% possible all
3.2-3.372.90.6541.1154350.5470.9610.65497.9
3.37-3.582.70.4081.8140720.3390.5930.40894
3.58-3.822.90.2612.8137350.2070.3640.26197.6
3.82-4.132.90.1674.3127760.1330.2360.16797.9
4.13-4.532.70.1126.3112390.0860.1510.11293.1
4.53-5.0630.0868.2107240.0650.1170.08698.8
5.06-5.842.80.0927.690870.0720.1280.09294.3
5.84-7.1630.1095.979470.0750.1370.10997.2
7.16-10.122.90.0415.359250.0290.0520.0494.1
10.12-57.56530.02920.133250.0190.0350.02994.7

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Processing

Software
NameVersionClassification
MOSFLMdata reduction
SCALA3.3.21data scaling
PHENIXrefinement
PDB_EXTRACT3.24data extraction
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.208→57.565 Å / Cross valid method: THROUGHOUT / σ(F): 1.21 / Phase error: 22.19 / Details: The twin fractions were adjusted during refinement
RfactorNum. reflection% reflection
Rfree0.2341 4535 5.13 %
Rwork0.1753 --
obs0.1802 88337 81.93 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Bsol: 76.2 Å2 / ksol: 0.34 e/Å3
Displacement parametersBiso max: 152.59 Å2 / Biso mean: 48.5489 Å2 / Biso min: 0.63 Å2
Refinement stepCycle: final / Resolution: 3.208→57.565 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms36617 0 248 0 36865
Biso mean--36.43 --
Num. residues----4660
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONf_bond_d0.002
X-RAY DIFFRACTIONf_angle_d0.501
X-RAY DIFFRACTIONf_chiral_restr0.039
X-RAY DIFFRACTIONf_plane_restr0.004
X-RAY DIFFRACTIONf_dihedral_angle_d5.783
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.218-3.27350.3176260.2412421X-RAY DIFFRACTION8
3.2735-3.3330.2352510.21311014X-RAY DIFFRACTION19
3.333-3.39710.3437910.20741760X-RAY DIFFRACTION33
3.3971-3.46640.30341580.20633007X-RAY DIFFRACTION57
3.4664-3.54170.32122570.20184662X-RAY DIFFRACTION88
3.5417-3.62410.27332300.18984652X-RAY DIFFRACTION87
3.6241-3.71470.27012560.18785017X-RAY DIFFRACTION94
3.7147-3.81510.23892680.18054949X-RAY DIFFRACTION94
3.8151-3.92730.27472560.1744991X-RAY DIFFRACTION94
3.9273-4.0540.23052940.17064938X-RAY DIFFRACTION92
4.054-4.19890.23542360.16684875X-RAY DIFFRACTION92
4.1989-4.36690.23062280.16244725X-RAY DIFFRACTION88
4.3669-4.56550.21072490.1574689X-RAY DIFFRACTION88
4.5655-4.8060.18012700.15355017X-RAY DIFFRACTION94
4.806-5.10690.21172570.16074970X-RAY DIFFRACTION93
5.1069-5.50070.23842840.1764894X-RAY DIFFRACTION91
5.5007-6.05340.26342240.20144607X-RAY DIFFRACTION86
6.0534-6.92730.25932530.20175012X-RAY DIFFRACTION94
6.9273-8.72010.20232200.18014840X-RAY DIFFRACTION90
8.7201-49.86240.18192620.17854894X-RAY DIFFRACTION90
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.4486-0.24510.21410.78690.15231.8871-0.00290.1730.3714-0.3559-0.0895-0.2194-0.74190.3465-0.07980.3632-0.12650.0790.40750.02190.13459.56135.1653-9.7158
20.7404-0.0219-0.76020.9153-0.19691.7958-0.0226-0.1027-0.00210.09160.0018-0.1462-0.13310.4576-0.09020.22960.09040.01440.3909-0.05880.111511.212920.98930.5403
30.8838-0.3995-0.4970.51110.130.58350.10040.2036-0.0234-0.2706-0.11360.0807-0.1222-0.15490.17550.47880.22530.02810.3216-0.08640.2198-7.391631.1736-1.032
41.2958-0.5860.31881.03240.35990.92060.0386-0.05490.10670.0333-0.03390.0778-0.3499-0.1998-0.01220.52270.11370.23190.4586-0.0940.3525-5.834637.627612.8083
50.14420.0234-0.5190.04550.08812.54250.15-0.10860.0416-0.1159-0.09960.0790.0331-0.1279-0.02310.7386-0.090.10260.46260.05010.5787-6.006240.37811.6582
60.1241-0.0543-0.04510.39950.13710.2285-0.0242-0.17540.02560.0723-0.0470.015-0.013-0.0783-0.11420.3920.14230.20290.29950.02870.1075-0.194612.70389.8486
72.1559-0.5864-0.31964.1017-1.14491.5658-0.00730.18840.3246-0.229-0.0006-0.1137-0.2324-0.03680.00290.60040.22720.11980.44320.05520.267-2.74529.6828-33.3477
81.13460.3329-0.23360.90960.42770.5411-0.05280.16080.0676-0.1882-0.0630.0948-0.0796-0.0093-0.17690.22720.12020.10320.3612-0.02420.1243-2.1655-6.8539-28.3641
91.51790.2094-0.12222.62550.19521.07860.00180.30610.0923-0.5997-0.07740.6175-0.3564-0.39570.07160.35980.1295-0.0210.4061-0.05060.2519-15.9373.9576-16.4982
100.28980.0114-0.24940.86240.74450.89230.1068-0.01890.13590.1106-0.0570.2289-0.1005-0.08960.01490.3050.01460.10350.3812-0.09430.1279-11.0499-7.8477-12.3076
110.689-0.320.13030.5346-0.10660.6983-0.04850.02360.2756-0.0185-0.0728-0.18410.06970.13030.08180.1582-0.08920.1150.16620.02170.2116-0.6823-38.6096-53.571
121.1884-0.13560.89790.1787-0.41951.7879-0.04480.12370.3420.1624-0.08210.0832-0.1735-0.1858-0.18620.2457-0.07720.19110.19-0.03790.3649-19.2944-32.1987-41.3924
130.27430.33240.11780.47560.23050.2135-0.0483-0.01070.0823-0.0043-0.05780.1456-0.06590.072-0.47770.2149-0.06480.1230.24860.0980.2064-9.5214-45.1638-38.3254
141.34430.5264-0.15611.8409-1.22222.5794-0.02180.3310.2535-0.1280.06060.1963-0.5257-0.2939-0.16060.8361-0.00610.16430.63220.1350.30254.1975-66.0032-76.8988
150.7285-0.1169-0.15271.00510.15110.9970.00630.3978-0.2664-0.4113-0.0594-0.03850.0085-0.16610.05760.3620.04990.09690.4358-0.08010.20489.2256-79.511-67.8761
161.35920.1888-0.27681.1407-0.24630.92430.17820.7068-0.142-0.42010.02130.4318-0.2058-0.4195-0.00930.33380.1621-0.07760.4978-0.12870.3957-11.6839-70.6478-60.2389
171.1466-0.4982-0.43870.88890.39680.8358-0.0199-0.0564-0.0920.14120.05990.10490.00880.09750.05350.39990.16020.23710.39230.03760.33165.6009-88.4757-51.2804
183.96942.06214.02981.40052.16684.57170.05360.8913-0.5198-0.18420.2304-1.09750.33331.3668-0.29130.52320.06350.13770.3662-0.08270.52419.8021-115.1397-52.3022
191.53120.3548-1.55470.2986-0.03842.0566-0.0601-0.0116-0.1045-0.01310.0077-0.13380.21790.14340.03570.17110.1474-0.01570.2679-0.08120.135414.1604-112.2129-61.8318
208.6505-5.4630.84915.4091-1.44262.8399-0.1470.1117-0.71540.0701-0.02810.87130.3045-0.52170.16680.23050.03860.13690.412-0.14750.56212.4235-120.0535-68.0832
214.0153-2.5987-4.95861.72973.08826.435-0.01590.1595-0.18850.1021-0.11340.25470.0465-0.26150.17650.49540.1022-0.14230.38390.0840.221511.6657-118.4142-53.5613
220.86040.18240.05662.3860.21520.02140.0096-0.3134-0.31350.20290.20280.23730.3654-0.1931-0.24770.44460.2068-0.01240.34870.08990.24556.3896-109.7452-59.2596
231.1370.1430.60890.43870.18630.5193-0.0165-0.1416-0.10370.0031-0.09580.10820.0183-0.18930.06140.15030.07780.10080.4469-0.04870.54483.0949-115.2024-57.0476
240.3784-0.12570.17952.46391.11220.6521-0.09290.0976-0.3081-0.1544-0.0896-0.04960.39360.13890.20.43670.19890.22710.4994-0.09280.5892-0.4539-105.0611-56.491
250.06550.6151-0.15426.3665-2.11371.12370.04740.03040.27950.11080.20580.5849-0.0796-0.4196-0.2810.3017-0.03870.24020.3065-0.01860.6239-5.5857-111.5797-62.2603
261.36252.12.28883.25173.53163.84580.03780.03420.0624-0.1086-0.01820.0584-0.04040.0197-0.01160.71160.0176-0.14210.9380.10010.6926-4.9226-108.0926-46.06
275.3379-2.9535-1.17566.36-1.48831.2292-0.0206-0.191-0.18430.13840.03350.05260.2473-0.1444-0.0180.74980.16820.04350.8403-0.07930.8345-8.5095-99.5451-51.7988
280.68351.30320.36242.48510.69130.19340.0686-0.22310.25410.3316-0.01950.3469-0.1597-0.1505-0.05510.88930.2917-0.05330.9722-0.28120.9447-13.3615-103.4718-59.2634
291.02550.01130.12071.01730.14110.8913-0.21810.0101-0.35090.0862-0.0195-0.23690.37810.2160.01330.19210.03430.19440.2596-0.02210.209629.2613-15.553-10.113
300.2871-0.09350.05450.88860.37610.3726-0.14350.0428-0.29670.09910.2524-0.57280.33660.5203-0.12460.57850.08970.2780.5201-0.01030.79444.6691-82.6608-49.691
312.0411-1.0031-0.91591.24190.24721.3144-0.1161-0.45030.21910.08410.0051-0.093-0.04010.2838-0.02140.25730.09980.06220.2229-0.00670.27131.9591-65.7179-39.9058
321.39290.2753-0.1520.4514-0.59280.9926-0.1393-0.4225-0.59870.4250.08420.21730.29350.19580.01220.48040.0812-0.03630.32880.06320.3629-48.6861-66.8734-65.1224
331.3847-0.61380.07921.8624-0.02441.9053-0.1447-0.3073-0.15110.3820.1392-0.15540.2470.018-0.03890.26320.141-0.07540.2760.05190.1537-41.6369-49.8163-66.5466
341.03940.4005-0.67922.14041.20313.36180.12570.1317-0.01-0.01820.0676-0.10910.04830.0733-0.01180.23760.04520.02390.2374-0.03550.0712-44.143-53.6362-75.7666
350.76160.23140.35310.9818-0.03680.7489-0.0383-0.077-0.09050.06660.0195-0.01120.08140.02550.0830.18220.08010.14470.2867-0.05480.216-52.5288-48.8097-78.8595
365.8791.4935-1.6472.246-1.68062.69010.0325-0.13150.09570.27340.11060.4253-0.1551-0.5222-0.13140.2575-0.00640.00730.3599-0.00380.1343-69.117-57.2154-74.3318
370.9525-0.12490.5151.18190.37311.14840.07370.216-0.0376-0.11440.035-0.15080.45130.34580.07730.36810.01490.16370.2494-0.01250.2872-54.9824-64.1501-82.2432
380.36340.9988-0.30262.9575-0.8780.2903-0.07810.2835-0.2613-0.03590.19130.20510.5006-0.397-0.10060.3699-0.06730.11010.4775-0.02160.4109-70.9761-65.2424-84.8408
390.4896-0.0332-0.30850.35960.1130.9493-0.14560.108-0.3240.0687-0.25810.26030.3735-0.22620.13720.5106-0.01290.12720.3926-0.11240.2485-63.7508-71.4385-88.764
400.1390.0352-0.09350.6250.06310.4007-0.11830.1942-0.1738-0.25150.02530.01720.0774-0.0356-0.03150.2539-0.00430.04360.3979-0.170.0708-57.135-42.2878-84.7786
411.62950.2325-0.42641.7188-0.18612.0619-0.0971-0.1527-0.23190.12810.01440.05290.4081-0.07970.24960.30660.00890.16650.3439-0.01340.1182-60.6949-39.1142-41.6045
421.0566-0.061-0.1870.40970.03790.7158-0.0872-0.062-0.22550.30230.00380.18060.1224-0.20810.09670.24780.06550.12580.3408-0.10810.1033-62.7277-25.3025-48.7699
431.04680.4554-0.10121.11170.32621.1071-0.23690.182-0.1146-0.0494-0.00840.45850.2076-0.15150.10830.18910.04150.13060.3944-0.05930.2754-69.8492-23.4025-62.3194
442.39480.9354-0.61790.66370.5623.2110.1157-0.2221-0.78260.20980.0519-0.20260.79520.21180.00240.3183-0.0578-0.11050.35930.11220.3576-60.6533-1.3641-15.2369
451.58870.1841-0.06992.25290.21661.58830.0428-0.2983-0.20950.40240.0503-0.10240.24910.2553-0.09570.1759-0.0073-0.01140.20.01510.1883-51.5529.324-19.0306
460.72490.2981-0.29340.5164-0.280.5503-0.0236-0.1613-0.13230.071-0.06230.09420.2151-0.0891-0.06710.2749-0.20170.12360.25350.0280.264-66.502610.7641-28.7916
471.60630.1999-0.80370.51420.14471.39220.00860.27520.1224-0.0613-0.028-0.02710.0031-0.0662-0.3470.2189-0.07120.21360.2434-0.07610.3792-82.93637.7502-29.84
480.40650.54870.05130.9211-0.04020.1453-0.12140.1697-0.1419-0.23440.10480.06630.0430.0027-0.05910.1271-0.09250.08010.1774-0.07860.2698-69.746911.9243-36.4526
491.14910.2817-0.95981.1398-1.11551.5279-0.1214-0.45660.03920.31490.0177-0.02520.01990.01970.13840.8097-0.24930.00830.6614-0.12240.3039-47.620743.24860.9755
501.51180.5685-0.36020.3985-0.32561.15530.0398-0.22370.02950.2809-0.1990.20560.2366-0.17660.10.3994-0.1330.01790.303-0.05520.3604-53.513750.7295-10.2631
511.979-0.4247-0.29511.7680.24961.0283-0.0597-0.48280.06150.3902-0.10410.37010.2015-0.25130.06340.5851-0.15670.00940.4916-0.05730.3029-67.90843.5369-13.7426
521.6973-0.3022-0.1540.78590.28092.34150.0397-0.2788-0.10560.365-0.10310.50390.1743-0.6389-0.02120.5345-0.2406-0.05110.58150.05760.4174-73.522237.9002-17.6337
532.241.26240.57432.07650.30960.7669-0.02160.19690.119-0.2032-0.03910.2567-0.18330.24280.02180.24220.01080.07170.2633-0.09950.2923-52.00158.9527-23.6762
540.8841.1540.46953.76291.46182.3918-0.06590.1759-0.1971-0.23450.1668-0.15840.08030.0314-0.27340.3813-0.0362-0.26540.3133-0.12890.6789-43.178186.2192-12.7511
550.38710.0496-0.1931.2061-0.15990.25470.00460.00590.03280.1284-0.02690.0211-0.045-0.0033-0.07360.37890.1339-0.2420.2389-0.16190.4487-48.715885.031-18.386
562.64580.91890.08769.76931.02350.34470.0046-0.4587-0.07230.6817-0.03380.0782-0.2129-0.02340.01210.382-0.031-0.10330.4112-0.22210.6815-54.856286.875-8.5689
570.474-0.0361-0.5750.00490.03810.7114-0.12090.06610.03160.05620.01970.0602-0.0704-0.19690.0770.44850.0198-0.07840.33680.140.4667-55.075984.3401-23.0943
583.4954-1.7977-1.03682.59631.9531.69990.0202-0.0014-0.1907-0.02080.05860.24790.0241-0.1614-0.03010.4475-0.01870.10390.2493-0.15350.5169-58.341775.1814-17.4056
593.2902-3.03761.17974.03111.89087.73060.0074-0.2306-0.19980.3022-0.069-0.02640.1912-0.01480.03160.3513-0.0705-0.03790.5618-0.01770.6532-63.503581.7534-11.4897
600.0002-0.0018-0.00190.09320.09330.0927-0.03840.121-0.2414-0.1067-0.06230.09910.2071-0.06380.04210.6064-0.094-0.26760.869-0.08470.7418-64.864574.2258-24.8044
611.13542.29221.34535.50253.94843.3301-0.0128-0.063-0.39380.2068-0.0186-0.68270.37950.51590.02961.2934-0.0659-0.14141.24680.11981.2127-71.052573.3006-13.8314
620.8933-0.0271-0.28590.81850.16051.4624-0.1435-0.05240.07850.0083-0.0259-0.21240.01850.03360.09040.19930.02490.03530.08050.09710.4201-28.4793-13.8744-64.1591
630.388-0.1312-0.14891.6930.80580.82660.0406-0.12950.1806-0.04620.095-0.618-0.29080.4443-0.24370.3586-0.0882-0.08630.50080.19450.6673-13.679853.6338-23.9779
640.94990.4169-0.07361.87760.65141.71250.07850.0877-0.1448-0.07590.1795-0.20840.17540.4128-0.16660.34130.21670.0540.75380.07080.4617-24.860236.8525-34.1527
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 102 )A2 - 102
2X-RAY DIFFRACTION2chain 'A' and (resid 103 through 199 )A103 - 199
3X-RAY DIFFRACTION3chain 'A' and (resid 200 through 243 )A200 - 243
4X-RAY DIFFRACTION4chain 'A' and (resid 244 through 337 )A244 - 337
5X-RAY DIFFRACTION5chain 'A' and (resid 338 through 372 )A338 - 372
6X-RAY DIFFRACTION6chain 'A' and (resid 373 through 437 )A373 - 437
7X-RAY DIFFRACTION7chain 'B' and (resid 1 through 63 )B1 - 63
8X-RAY DIFFRACTION8chain 'B' and (resid 64 through 243 )B64 - 243
9X-RAY DIFFRACTION9chain 'B' and (resid 244 through 324 )B244 - 324
10X-RAY DIFFRACTION10chain 'B' and (resid 325 through 441 )B325 - 441
11X-RAY DIFFRACTION11chain 'C' and (resid 2 through 243 )C2 - 243
12X-RAY DIFFRACTION12chain 'C' and (resid 244 through 337 )C244 - 337
13X-RAY DIFFRACTION13chain 'C' and (resid 338 through 439 )C338 - 439
14X-RAY DIFFRACTION14chain 'D' and (resid 1 through 64 )D1 - 64
15X-RAY DIFFRACTION15chain 'D' and (resid 65 through 243 )D65 - 243
16X-RAY DIFFRACTION16chain 'D' and (resid 244 through 373 )D244 - 373
17X-RAY DIFFRACTION17chain 'D' and (resid 374 through 441 )D374 - 441
18X-RAY DIFFRACTION18chain 'F' and (resid 43 through 49 )F43 - 49
19X-RAY DIFFRACTION19chain 'F' and (resid 50 through 59 )F50 - 59
20X-RAY DIFFRACTION20chain 'F' and (resid 60 through 68 )F60 - 68
21X-RAY DIFFRACTION21chain 'F' and (resid 69 through 82 )F69 - 82
22X-RAY DIFFRACTION22chain 'F' and (resid 83 through 92 )F83 - 92
23X-RAY DIFFRACTION23chain 'F' and (resid 93 through 115 )F93 - 115
24X-RAY DIFFRACTION24chain 'F' and (resid 116 through 125 )F116 - 125
25X-RAY DIFFRACTION25chain 'F' and (resid 126 through 135 )F126 - 135
26X-RAY DIFFRACTION26chain 'F' and (resid 136 through 148 )F136 - 148
27X-RAY DIFFRACTION27chain 'F' and (resid 149 through 158 )F149 - 158
28X-RAY DIFFRACTION28chain 'F' and (resid 159 through 167 )F159 - 167
29X-RAY DIFFRACTION29chain 'E' and (resid 14 through 253 )E14 - 253
30X-RAY DIFFRACTION30chain 'E' and (resid 254 through 395 )E254 - 395
31X-RAY DIFFRACTION31chain 'E' and (resid 396 through 543 )E396 - 543
32X-RAY DIFFRACTION32chain 'G' and (resid 2 through 88 )G2 - 88
33X-RAY DIFFRACTION33chain 'G' and (resid 89 through 128 )G89 - 128
34X-RAY DIFFRACTION34chain 'G' and (resid 129 through 160 )G129 - 160
35X-RAY DIFFRACTION35chain 'G' and (resid 161 through 199 )G161 - 199
36X-RAY DIFFRACTION36chain 'G' and (resid 200 through 223 )G200 - 223
37X-RAY DIFFRACTION37chain 'G' and (resid 224 through 273 )G224 - 273
38X-RAY DIFFRACTION38chain 'G' and (resid 274 through 311 )G274 - 311
39X-RAY DIFFRACTION39chain 'G' and (resid 312 through 372 )G312 - 372
40X-RAY DIFFRACTION40chain 'G' and (resid 373 through 437 )G373 - 437
41X-RAY DIFFRACTION41chain 'H' and (resid 1 through 64 )H1 - 64
42X-RAY DIFFRACTION42chain 'H' and (resid 65 through 295 )H65 - 295
43X-RAY DIFFRACTION43chain 'H' and (resid 296 through 441 )H296 - 441
44X-RAY DIFFRACTION44chain 'I' and (resid 2 through 47 )I2 - 47
45X-RAY DIFFRACTION45chain 'I' and (resid 48 through 160 )I48 - 160
46X-RAY DIFFRACTION46chain 'I' and (resid 161 through 273 )I161 - 273
47X-RAY DIFFRACTION47chain 'I' and (resid 274 through 311 )I274 - 311
48X-RAY DIFFRACTION48chain 'I' and (resid 312 through 439 )I312 - 439
49X-RAY DIFFRACTION49chain 'J' and (resid 1 through 127 )J1 - 127
50X-RAY DIFFRACTION50chain 'J' and (resid 128 through 243 )J128 - 243
51X-RAY DIFFRACTION51chain 'J' and (resid 244 through 324 )J244 - 324
52X-RAY DIFFRACTION52chain 'J' and (resid 325 through 373 )J325 - 373
53X-RAY DIFFRACTION53chain 'J' and (resid 374 through 441 )J374 - 441
54X-RAY DIFFRACTION54chain 'L' and (resid 43 through 68 )L43 - 68
55X-RAY DIFFRACTION55chain 'L' and (resid 69 through 92 )L69 - 92
56X-RAY DIFFRACTION56chain 'L' and (resid 93 through 101 )L93 - 101
57X-RAY DIFFRACTION57chain 'L' and (resid 102 through 115 )L102 - 115
58X-RAY DIFFRACTION58chain 'L' and (resid 116 through 125 )L116 - 125
59X-RAY DIFFRACTION59chain 'L' and (resid 126 through 135 )L126 - 135
60X-RAY DIFFRACTION60chain 'L' and (resid 136 through 158 )L136 - 158
61X-RAY DIFFRACTION61chain 'L' and (resid 159 through 167 )L159 - 167
62X-RAY DIFFRACTION62chain 'K' and (resid 14 through 253 )K14 - 253
63X-RAY DIFFRACTION63chain 'K' and (resid 254 through 388 )K254 - 388
64X-RAY DIFFRACTION64chain 'K' and (resid 389 through 543 )K389 - 543

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